Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (13): 2268-2279.doi: 10.3864/j.issn.0578-1752.2019.13.007

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Microbial Transformation Process of Straw-Derived C in Two Typical Paddy Soils

QIU CunPu1,2,CHEN XiaoFen3,LIU Ming1,2,LI WeiTao1,WU Meng1,JIANG ChunYu1,FENG YouZhi1,2(),LI ZhongPei1,2()   

  1. 1 State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008
    2 University of Chinese Academy of Sciences, Beijing 100049
    3 Soil and Fertilizer & Resources and Environment Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200;
  • Received:2018-12-22 Accepted:2019-03-11 Online:2019-07-01 Published:2019-07-11
  • Contact: YouZhi FENG,ZhongPei LI E-mail:yzfeng@issas.ac.cn;zhpli@issas.ac.cn

RichHTML

11

PDF

627

Abstract:

【Objective】 The straw degradation rate, microbial community structure changes and functional microbial community composition involved in straw decomposition in soils were researched, and the research results could provide the theoretical foundation for revealing microbial mechanism of the soil organic matter transformation and accumulation. 【Method】 Two typical subtropical paddy soil in China, including Changshu Wushan soil and Yingtan Red paddy soil, were collected as the research materials. We anaerobically incubated the soils with/without 13C-enriched rice straw for 38 days. Gaseous samples were regularly collected to investigate mineralization rate of straw in dynamic changes. The soil samples were collected to analyze the dynamic changes of the microbial community composition related to straw decomposition by using 13C-PLFA-SIP technology. 【Result】 At the early stage before day 12 of the anaerobic culture, straw degraded slowly, and straw had positive priming effect on soil organic matter (SOM). At the stage of day 12-18, straw degraded rapidly and then the rate tended to be slow after day 18. At the end of incubation, straw mineralization rate was 24% and 33% in Red paddy soil and Wushan soil, respectively. The contribution of straw C to C efflux increased with incubation time, which was 53%-60% and 54%-57% to CO2 and CH4 efflux, respectively. The microbial biomass and activity were improved in the soil with straw, and the microbial activity in Wushan soil was higher than that in Red paddy soil. During straw degradation, 16:0 (general bacteria) was the main groups. i16:0, i15:0 (G + bacteria) and 18:1ω9c (fungi) were also important microbial groups involved in straw degradation. The relative abundance of straw-derived gram-positive (G +) bacteria and actinomycetes increased and gram-positive (G -) bacteria decreased with incubation time. The proportions of straw-derived PLFAs were 27%-32% and 18%-24% in Red paddy soil and Wushan soil PLFAs, respectively. The straw utilization efficiency was higher in fungi and general bacteria, while G - bacterial and actinomycetes PLFAs were preferentially linked to extant soil organic matter (SOM) mineralization. The microbial community composition was different between Wushan soil and Red paddy soil with rice straw. The straw-derived microbial community composition was similar in two soils, but the SOM-derived microorganisms were differences. 【Conclusion】 The mineralization of straw C lagged behind extant SOM during anaerobic straw degradation. The microbial activity and diversity in soil were important factors influencing the efficiency of straw mineralization. After adding straw in soil, it’s showed differences from the microbial community composition, which were mainly involved in the differences between SOM-derived microorganisms, and SOM was an important factor leading to these differences.

Key words: rice straw degradation, 13C-PLFA-SIP, paddy soil, microbial community

Table 1

Soil properties in the study"

水稻土
Paddy soil		 pH δ13C 有机质
Soil organic matter（g·kg-1）		 全氮
Total N（g·kg-1）		 全磷
Total P（g·kg-1）		 全钾
Total K（g·kg-1）		 C/N
鹰潭红壤性水稻土
Yingtan Red paddy soil		 5.1 -27‰ 23.74 1.66 0.62 17.10 8.29
常熟乌栅土
Changshu Wushan soil		 6.6 -29‰ 35.88 2.19 0.96 17.07 9.5

Fig. 1

Cumulative production of CO2 (a) and CH4 (b) in paddy soils with / without straw additions. The production in soils with straws was further partitioned as straw- and soil organic matter (SOM)-derived; rice straw mineralization (c) and its contribution to total CO2 and CH4 production (d) during the anaerobic incubation The abbreviations “YT” and “CS” in the legend indicates Yingtan Red paddy soil and Changshu Wushan soil, respectively. The same as below"

Fig. 2

Priming effects of CO2 (a), CH4 (b) and CO2 plus CH4 (c)"

Fig. 3

Changes in PLFAs composition of different paddy soils with and without straw additions during the anaerobic incubation The abbreviations “CK” and “RS” indicates soils without and with straw addition, respectively"

Fig. 4

Principal component analysis (PCA) on PLFAs compositions of soils incubated with and without rice straw The abbreviations “CK” and “RS” indicates soils without and with straw addition, respectively. The numbers following the letters are incubation time in days"

Fig. 5

Incorporations of C derived from straw in individual PLFA fractions in soils incubated with rice straws"

Fig. 6

Contribution of straw-derived C to PLFAs in soils incubated with rice straw"

Table 2

Relative abundance of straw-derived C incorporated PLFAs composition in soils incubated with rice straw"

土壤
Soil		 相对丰度
Relative abundance (%)		 一般细菌
General bacteria		 G+细菌
G+ bacteria		 G-细菌
G- bacteria		 真菌
Fungi		 放线菌
Actinomycete
YT 3d 59.98±2.31 15.77±0.56 2.76±0.1 21.43±0.81 0.07±0.02
9d 62.84±3.5 14.32±0.02 6.26±0.45 16.32±0.13 0.27±0.03
18d 76.57±3.11 13.05±0.36 0.32±0.02 10.05±0.16 0.01±0.00
38d 60.27±2.71 28.14±1.6 0.67±0.02 9.82±0.16 1.09±0.07
CS 3d 50.43±1.36 14.59±0.77 8.99±0.34 25.27±1.52 0.72±0.03
9d 46.56±1.96 27.22±1.37 13.64±0.29 10.88±0.72 1.69±0.19
18d 54.47±2.75 30.89±1.06 3.58±0.08 8.64±0.2 2.42±0.05
38d 53.68±1.55 29.81±0.09 4.53±0.07 6.77±0.16 5.21±0.61

Fig. 7

Principal component analysis on incorporation patterns of C in individual PLFA fractions The abbreviations “RS” and “SOM” indicates rice straw and soil organic matter derived, respectively. The numbers following the letters are incubation time in days"

[1] CHEN X F, LIU M, KUZYAKOV Y, LI W T, LIU J, JIANG C Y, WU M, LI Z P . Incorporation of rice straw carbon into dissolved organic matter and microbial biomass along a 100-year paddy soil chronosequence. Applied Soil Ecology, 2018,130:84-90.
doi: 10.1016/j.apsoil.2018.06.004
[2] SWIFT M H O, ANDERSON J . Decomposition In Terrestrial Ecosystems. Los Angeles: Univeristy of California Press, 1979.
[3] RUI J, PENG J, LU Y . Succession of bacterial populations during plant residue decomposition in rice field soil. Applied and Environmental Microbiology, 2009,75(14):4879-4886.
doi: 10.1128/AEM.00702-09
[4] PEI J, LI H, LI S, AN T, FARMER J, FU S, WANG J . Dynamics of maize carbon contribution to soil organic carbon in association with soil type and fertility level. PLoS One, 2015,10(3):e0120825.
doi: 10.1371/journal.pone.0120825
[5] TANG S R, CHENG W G, HU R G, GUIGUE J, KIMANI S M, TAWARAYA K, XU X K . Simulating the effects of soil temperature and moisture in the off-rice season on rice straw decomposition and subsequent CH4 production during the growth season in a paddy soil. Biology and Fertility of Soils, 2016,52(5):739-748.
doi: 10.1007/s00374-016-1114-8
[6] WANG X, SUN B, MAO J, SUI Y, CAO X . Structural convergence of maize and wheat straw during two-year decomposition under different climate conditions. Environmental Science and Technology, 2012,46(13):7159-7165.
doi: 10.1021/es300522x
[7] 王玉竹, 周萍, 王娟, 马蓓, 刘翊涵, 吴金水 . 亚热带几种典型稻田与旱作土壤中外源输入秸秆的分解与转化差异. 生态学报, 2017,37(19):6457-6465.
WANG Y Z, ZHOU P, WANG J, MA B, LIU Y H, WU J S . Decomposition and transformation of input straw in several typical paddy and upland soils in subtropical China. Acta Ecologica Sinica, 2017,37(19):6457-6465. (in Chinese)
[8] MATSUYAMA T, NAKAJIMA Y, MATSUYA K, IKENAGA M, ASAKAWA S, KIMURA M . Bacterial community in plant residues in a Japanese paddy field estimated by RFLP and DGGE analyses. Soil Biology and Biochemistry, 2007,39(2):463-472.
doi: 10.1016/j.soilbio.2006.08.016
[9] 喻曼, 曾光明, 陈耀宁, 郁红艳, 黄丹莲, 陈芙蓉 . PLFA法研究稻草固态发酵中的微生物群落结构变化. 环境科学, 2007,11:2603-2608.
YU M, ZENG G M, CHEN Y N, YU H Y, HUANG D L, CHEN F R . Microbial community changes during the degradation of sraw using phospholipid fatty acid analysis. Environmental Science,2007, 11:2603-2608. (in Chinese)
[10] LI P, LI Y C, ZHENG X Q, DING L N, MING F, PAN A H, LV W G, TANG X M . Rice straw decomposition affects diversity and dynamics of soil fungal community, but not bacteria. Journal of Soils and Sediments, 2018,18(1):248-258.
doi: 10.1007/s11368-017-1749-6
[11] BASTIAN F, BOUZIRI L, NICOLARDOT B, RANJARD L . Impact of wheat straw decomposition on successional patterns of soil microbial community structure. Soil Biology & Biochemistry, 2009,41(2):262-275.
[12] BLAGODATSKAYA E, KUZYAKOV Y . Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure: critical review. Biology and Fertility of Soils, 2008,45(2):115-131.
doi: 10.1007/s00374-008-0334-y
[13] BAI Z, LIANG C, BODE S, HUYGENS D, BOECKX P . Phospholipid C-13 stable isotopic probing during decomposition of wheat residues. Applied Soil Ecology, 2016,98:65-74.
doi: 10.1016/j.apsoil.2015.09.009
[14] 窦森, 张晋京, LICHTFOUSE E, 曹亚澄, . 用δ 13C方法研究玉米秸秆分解期间土壤有机质数量动态变化. 土壤学报, 2003,3:328-334.
DOU S, ZHANG J J, LICHTFOUSE E, CAO Y C . Study on dynamic change of soil organic matter during corn stalk decomposition by δ 13C method. Acta Pedologica Sinica, 2003,3:328-334. (in Chinese)
[15] PAN F X, LI Y Y, CHAPMAN S J, KHAN S, YAO H Y . Microbial utilization of rice straw and its derived biochar in a paddy soil. Science of the Total Environment, 2016,559:15-23.
doi: 10.1016/j.scitotenv.2016.03.122
[16] CROSSMAN Z M, ABRAHAM F, EVERSHED R P . Stable isotope pulse-chasing and compound specific stable carbon isotope analysis of phospholipid fatty acids to assess methane oxidizing bacterial populations in landfill cover soils. Environmental Science & Technology, 2004,38(5):1359-1367.
[17] AOYAMA M, ANGERS D A, N'DAYEGAMIYE A, BISSONNETTE N . Metabolism of C-13-labeled glucose in aggregates from soils with manure application. Soil Biology & Biochemistry, 2000,32(3):295-300.
[18] WILLIAMS M A, MYROLD D D, BOTTOMLEY P J . Carbon flow from C-13-labeled straw and root residues into the phospholipid fatty acids of a soil microbial community under field conditions. Soil Biology & Biochemistry, 2006,38(4):759-768.
[19] BUYER J S, TEASDALE J R, ROBERTS D P, ZASADA I A, MAUL J E . Factors affecting soil microbial community structure in tomato cropping systems. Soil Biology & Biochemistry, 2010,42(5):831-841.
[20] BLIGH E G, DYER W J . A rapid method of toal lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 1959,37(8):911-917.
doi: 10.1139/y59-099
[21] ZHANG H, DING W, YU H, HE X . Carbon uptake by a microbial community during 30-day treatment with C-13-glucose of a sandy loam soil fertilized for 20 years with NPK or compost as determined by a GC-C-IRMS analysis of phospholipid fatty acids. Soil Biology & Biochemistry, 2013,57:228-236.
[22] ZELLES L . Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: A review. Biology and Fertility of Soils, 1999,29(2):111-129.
doi: 10.1007/s003740050533
[23] YE R Z, DOANE T A, MORRIS J, HORWATH W R . The effect of rice straw on the priming of soil organic matter and methane production in peat soils. Soil Biology & Biochemistry, 2015,81:98-107.
[24] MURASE J, MATSUI Y, KATOH M, SUGIMOTO A, KIMURA M . Incorporation of 13C-labeled rice-straw-derived carbon into microbial communities in submerged rice field soil and percolating water . Soil Biology & Biochemistry, 2006,38(12):3483-3491.
[25] JIN S, CHEN H . Near-infrared analysis of the chemical composition of rice straw. Industrial Crops and Products, 2007,26(2):207-211.
doi: 10.1016/j.indcrop.2007.03.004
[26] KIMURA M, MIYAKI M, FUJINAKA K, MAIE N . Microbiota responsible for the decomposition of rice straw in a submerged paddy soil estimated from phospholipid fatty acid composition. Soil Science and Plant Nutrition, 2001,47(3):569-578.
doi: 10.1080/00380768.2001.10408420
[27] 吴景贵, 王明辉, 万忠梅, 姜亦梅, 吴江 . 玉米秸秆腐解过程中形成胡敏酸的组成和结构研究. 土壤学报, 2006,143(13):443-451.
WU J G, WANG M H, WANG Z M, JIANG Y M, WU J . Chemical composition and structure of humic acid from composted corn stalk residue. Acta Pedologica Sinica, 2006, 143(13):443-451. (in Chinese)
[28] ZHU Z K, GE T D, LUO Y, LIU S L, XU X L, TONG C L, SHIBISTOVA O, GUGGENBERGER G, WU J S . Microbial stoichiometric flexibility regulates rice straw mineralization and its priming effect in paddy soil. Soil Biology & Biochemistry, 2018,121:67-76.
[29] YE R Z, HORWATH W R . Influence of rice straw on priming of soil C for dissolved organic C and CH4 production. Plant and Soil, 2017,417(1/2):231-241.
doi: 10.1007/s11104-017-3254-5
[30] FONTAINE S, BARDOUX G, BENEST D, VERDIER B, MARIOTTI A, ABBADIE L . Mechanisms of the priming effect in a savannah soil amended with cellulose. Soil Science Society of America Journal, 2004,68(1):125-131.
doi: 10.2136/sssaj2004.1250
[31] AN T T, SCHAEFFER S, ZHUANG J, RADOSEVICH M, LI S Y, LI H, PEI J B, WANG J K . Dynamics and distribution of 13C-labeled straw carbon by microorganisms as affected by soil fertility levels in the black soil region of Northeast China. Biology and Fertility of Soils, 2015, 51:605-613.
[32] FONTAINE S, MARIOTTI A, ABBADIE L . The priming effect of organic matter: a question of microbial competition? Soil Biology & Biochemistry, 2003,35(6):837-843.
[33] 王旭东, 陈鲜妮, 王彩霞, 田霄鸿, 吴发启 . 农田不同肥力条件下玉米秸秆腐解效果. 农业工程学报, 2009,25(10):252-257.
WANG X D, CHEN X N, WANG C X, TIAN X H, WU Q F . Decomposition of corn stalk in cropland with different fertility. Transactions of the CSAE, 2009,25(10):252-257. (in Chinese)
[34] 孙星, 刘勤, 王德建, 张斌 . 长期秸秆还田对土壤肥力质量的影响. 土壤, 2007,39(5):782-786.
SUN X, LIU Q, WANG D J, ZHANG B . Effect of long-term straw application on soil fertility. Soil, 2007,39(5):782-786. (in Chinese)
[35] LI D D, CHEN L, XU J S H, MA L, Olk Dan C, ZHAO B Z, ZHANG J B, XIN X L . Chemical nature of soil organic carbon under different long-term fertilization regimes is coupled with changes in the bacterial community composition in a Calcaric Fluvisol. Biology and Fertility of Soils, 2018,54(8):999-1012.
doi: 10.1007/s00374-018-1319-0
[36] . MARSCHNER P, KANDELER E, MARSCHNER B . Structure and function of the soil microbial community in a long-term fertilizer experiment. Soil Biology & Biochemistry, 2003,35(3):453-461.
[37] MOORE-KUCERA J, DICK R P . Application of C-13-labeled litter and root materials for in situ decomposition studies using phospholipid fatty acids. Soil Biology & Biochemistry, 2008,40(10):2485-2493.
[38] ELFSTRAND S, LAGERLOF J, HEDLUND K, MARTENSSON A . Carbon routes from decomposing plant residues and living roots into soil food webs assessed with C-13 labelling. Soil Biology & Biochemistry, 2008,40(10):2530-2539.
[39] CONRAD R . Contribution of hydrogen to methane production and control of hydrogen concentrations in methanogenic soils and sediments. Fems Microbiology Ecology, 1999,28(3):193-202.
doi: 10.1111/fem.1999.28.issue-3
[40] NOLL M, MATTHIES D, FRENZEL P, DERAKSHANI M, LIESACK W . Succession of bacterial community structure and diversity in a paddy soil oxygen gradient. Environmental Microbiology, 2005,7(3):382-395.
doi: 10.1111/emi.2005.7.issue-3
[1] GAO JiaRui,FANG ShengZhi,ZHANG YuLing,AN Jing,YU Na,ZOU HongTao. Characteristics of Organic Nitrogen Mineralization in Paddy Soil with Different Reclamation Years in Black Soil of Northeast China [J]. Scientia Agricultura Sinica, 2022, 55(8): 1579-1588.
[2] GONG XiaoYa,SHI JiBo,FANG Ling,FANG YaPeng,WU FengZhi. Effects of Flooding on Soil Chemical Properties and Microbial Community Composition on Farmland of Continuous Cropped Pepper [J]. Scientia Agricultura Sinica, 2022, 55(12): 2472-2484.
[3] SHAO MeiQi,ZHAO WeiSong,SU ZhenHe,DONG LiHong,GUO QingGang,MA Ping. Effect of Bacillus subtilis NCD-2 on the Growth of Tomato and the Microbial Community Structure of Rhizosphere Soil Under Salt Stress [J]. Scientia Agricultura Sinica, 2021, 54(21): 4573-4584.
[4] REN HaiYing,ZHOU HuiMin,QI XingJiang,ZHENG XiLiang,YU ZhePing,ZHANG ShuWen,WANG ZhenShuo. Effects of Paclobutrazol on Soil and Endophytic Microbial Community Structure of Bayberry [J]. Scientia Agricultura Sinica, 2021, 54(17): 3752-3765.
[5] Kai LIU,Jia LIU,XiaoFen CHEN,WeiTao LI,ChunYu JIANG,Meng WU,JianBo FAN,ZhongPei LI,Ming LIU. Seasonal Variation and Differences of Microbial Biomass Phosphorus in Paddy Soils Under Long-Term Application of Phosphorus Fertilizer [J]. Scientia Agricultura Sinica, 2020, 53(7): 1411-1418.
[6] XU Meng,XU LiJun,CHENG ShuLan,FANG HuaJun,LU MingZhu,YU GuangXia,YANG Yan,GENG Jing,CAO ZiCheng,LI YuNa. Responses of Soil Organic Carbon Fractionation and Microbial Community to Nitrogen and Water Addition in Artificial Grassland [J]. Scientia Agricultura Sinica, 2020, 53(13): 2678-2690.
[7] LI DongChu,HUANG Jing,MA ChangBao,XUE YanDong,GAO JuSheng,WANG BoRen,ZHANG YangZhu,LIU KaiLou,HAN TianFu,ZHANG HuiMin. Spatio-Temporal Variations of Soil Organic Matter in Paddy Soil and Its Driving Factors in China [J]. Scientia Agricultura Sinica, 2020, 53(12): 2410-2422.
[8] ZHANG MengYang,XIA Hao,LÜ Bo,CONG Ming,SONG WenQun,JIANG CunCang. Short-Term Effect of Biochar Amendments on Total Bacteria and Ammonia Oxidizers Communities in Different Type Soils [J]. Scientia Agricultura Sinica, 2019, 52(7): 1260-1271.
[9] Lü ZhenZhen,LIU XiuMei,ZHONG JinFeng,LAN XianJin,HOU HongQian,JI JianHua,FENG ZhaoBin,LIU YiRen. Effects of Long-Term Fertilization on Mineralization of Soil Organic Carbon in Red Paddy Soil [J]. Scientia Agricultura Sinica, 2019, 52(15): 2636-2645.
[10] WeiFu PENG, WeiSheng LÜ, Shan HUANG, YongJun ZENG, XiaoHua PAN, QingHua SHI. Effects of Soil Fertility on Rice Yield and Nitrogen Use Efficiency in a Red Paddy Soil [J]. Scientia Agricultura Sinica, 2018, 51(18): 3614-3624.
[11] WANG QingFeng, JIANG Xin, MA MingChao, GUAN DaWei, ZHAO BaiSuo, WEI Dan, CAO FengMing, LI Li, LI Jun. Influence of Long-Term Nitrogen and Phosphorus Fertilization on Arbuscular Mycorrhizal Fungi Community in Mollisols of Northeast China [J]. Scientia Agricultura Sinica, 2018, 51(17): 3315-3324.
[12] CHEN XiaoFen, LIU Ming, JIANG ChunYu, WU Meng, LI ZhongPei. Organic Carbon Mineralization in Aggregate Fractions of Red Paddy Soil Under Different Fertilization Treatments [J]. Scientia Agricultura Sinica, 2018, 51(17): 3325-3334.
[13] WANG XiaoLi, GUO Zhen, DUAN JianJun, ZHOU ZhiGang, LIU YanLing, ZHANG YaRong. The Changes of Organic Carbon and Its Fractions in Yellow Paddy Soils Under Long-Term Fertilization [J]. Scientia Agricultura Sinica, 2017, 50(23): 4593-4601.
[14] CHENG YanHong, HUANG QianRu, WU Lin, HUANG ShangShu, ZHONG YiJun, SUN YongMing, ZHANG Kun, ZHANG XinLiang. Effects of Straw Mulching and Vetiver Grass Hedgerows on Soil Enzyme Activities and Soil Microbial Community Structure in Red Soil Sloping Land [J]. Scientia Agricultura Sinica, 2017, 50(23): 4602-4612.
[15] WANG YingYan, LU ShengE, LI YueFei, TU ShiHua, ZHANG XiaoPing, GU YunFu. Response of Anammox Bacteria Community Structure and Vertical Distribution to Different Long-Term Fertilizations in Calcareous Purple Paddy Soil [J]. Scientia Agricultura Sinica, 2017, 50(16): 3155-3163.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd