Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (12): 2468-2473.doi: 10.3864/j.issn.0578-1752.2012.12.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Analysis of Duck CD8α Gene Promoter and Its Transcriptional Activity

 XU  Qi, CHEN  Yang, HUANG  Zheng-Yang, ZHANG  Yang, CHEN  Chang-Yi, ZHAO  Rong-Xue, LI  Xiu, DUAN  Xiu-Jun, CHEN  Guo-Hong   

  1. 1.江苏省动物遗传繁育与分子设计重点实验室，江苏扬州  225009
    2.江西农业大学动物科学技术学院，江西南昌 330045
    3.国家水禽种质资源基因库，江苏泰州  225300
  • Received:2011-07-20 Online:2012-06-15 Published:2011-11-14

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Abstract: 【Objective】 This experiment was conducted to explore the function determination and expression regulation of CD8α gene in ducks by analysis of transcriptional activity of its promoter. 【Method】 The promoter missing mutants were constructed（-625/-1 bp, -1110/-1 bp, -1413/-1 bp, -2151/-1 bp）accroding to the sequence of CD8α gene promoter, and then subcloned into pGL3 basic vectors to construct luciferase reporter gene vectors, respectively. The recombinant vectors were transfected into DT40 cells with Lipofectamine 2000, and the transcriptional activities were detected. 【Result】Results of the study indicated that CD8α gene promoter had obviously promoter activity. The sequence from -1 110 to -625 bp of 5′ flanking region had the strongest promoter activities, including two positive (-625/-1 bp and -1 110/-625 bp) regulatory domains. 【Conclusion】 The luciferase reporter gene eukaryotic expression vector with CD8α gene promoter sequences was constructed successfully using deletion mutation and its major regulatory regions were found, which play an important role for analyzing the promoter activity and transcriptional regulation mechanism.

Key words: duck, CD8&alpha, gene, promoter, transcriptional activity

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