Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (10): 2022-2030.doi: 10.3864/j.issn.0578-1752.2012.10.015

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Cloning and Sequence Analysis of the Transferrin Receptor 2 Gene from Yanbian Yellow Cattle

 ZHANG  Li-Chun, JIN  Hai-Guo, LI  Zhao-Zhi, REN  Chun-Yu, CAO  Yang, ZHOU  Guo-Li, JIN  Xin   

  1. 1.吉林省农业科学院畜牧科学分院, 吉林公主岭 136100
    2.吉林大学白求恩第一医院，长春 130021
    3.吉林省延边州动物疫病预防控制中心，吉林延吉 133000
    4.聊城大学生命科学学院, 山东聊城  252059；5延边大学农学院，吉林延吉 133002
  • Received:2011-09-28 Online:2012-05-15 Published:2012-01-09

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Abstract: 【Objective】 The transferrin receptor 2 (TfR2) gene was cloned from Yanbian Yellow cattle, the sequence was analyzed by bioinformatics software and the tissue expression distribution was tested by semi-quantitative reverse transcription and polymerase chain reaction (SqRT-PCR).【Method】 The total RNA was extracted from Yanbian Yellow cattle liver. The TfR2 gene was cloned by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The TfR2 gene was analyzed by bioinformatic softwares and the TfR2 gene tissue distribution was tested by SqRT-PCR in the end. 【Result】 The complete CDs and 3′ UTR region of TfR2 gene was cloned (GenBank accession: GU553087). The TfR2 gene was 2901 bp in length and contained a 2412 -nucleotides-long open reading frame (ORF) which encoded a protein of 803 amino acid residues. The sequence analysis result showed that Yanbian Yellow cattle TfR2 gene has over 80% homology with mammals in nucleotides, the amino acid sequences of TfR2 gene from different mammals were conserved, especially some amino acid sites which affect its function in human were highly conserved. The protein functional domain analysis showed that TfR2 gene from different mammals were highly conserved. The expression profiles analysis showed that the TfR2 gene was highly expressed in liver, constitutively expressed in spleen, heart, kidney and intestine (duodenum).【conclusion】 The TfR2 gene from Yanbian Yellow cattle is highly homologous with other mammals, the functional domain and tissue distribution are highly conserved in different mammals.

Key words: Yanbian Yellow cattle, TfR2 gene, RACE

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