Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (4): 726-733.doi: 10.3864/j.issn.0578-1752.2012.04.013

• HORTICULTURE • Previous Articles     Next Articles

Analysis of Genetic Diversity and Construction of Fingerprint of Vriesea by ISSR

 GE  Ya-Ying, ZHANG  , FEI  , SHEN  Xiao-Lan, LIU  Jian-Xin, WANG  Wei-Yong, TIAN  Dan-Qing, ZHANG  , ZHI    

  1. 1.浙江省农业科学院花卉研究开发中心，杭州 311202
  • Received:2011-08-23 Online:2012-02-15 Published:2011-12-01

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Abstract: 【Objective】 The purpose of this experiment is to study the genetic diversity and phylogenetic relationship of Vriesea cultivars and establish their DNA fingerprints, which will provide some guidance for crossbreeding and cultivar identification of ornamental bromeliads in Vriesea. 【Method】 Inter-simple sequence repeat（ISSR）was conducted to assess the genetic diversity of 41 Vriesea cultivars and the DNA fingerprints of the cultivars were established.【Result】Among the 41 Vriesea cultivars, 12 ISSR primers produced 132 loci, of which 125 were polymorphisms, and the percentage of polymorphic loci was up to 94.70%. The average value of the effective number of alleles, Nei’s genetic diversity and Shannon’s information index was 1.5311, 0.3101 and 0.4668, respectively. The similarity coefficient between the 41 Vriesea cultivars ranged from 0.3561 to 0.9394. Based on the unweighted pari group method with arithmetic average (UPGMA) and ISSR molecular markers, the 41 Vriesea cultivars were divided into two groups at the similarity coefficient of 0.601. In addition, the DNA fingerprints for the Vriesea cultivars were established by 4 polymorphic ISSR primers.【Conclusion】There existed abundant genetic diversity among 41 Vriesea cultivars and their relationships were significantly correlated with leaf stripe. ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for Vriesea cultivars.

Key words: Vriesea, ISSR, genetic diversity, fingerprint

[1]朱根发, 李冬梅, 郭振飞. 大花蕙兰遗传多样性及亲缘关系的AFLP分析. 园艺学报, 2007, 34(2): 417-424.

Zhu G F, Li D M, Guo Z F. Genetic diversity and relationship of hybrid Cymbidium based on AFLP markers. Acta Horticulturae Sinica, 2007, 34(2): 417-424. (in Chinese)

[2]唐开学, 邱显钦, 张  颢, 李树发, 王其刚, 蹇洪英, 鄢  波, 黄兴奇. 云南蔷薇属部分种质资源的SSR遗传多样性研究. 园艺学报, 2008, 35(8):1227-1232.

Tang K X, Qiu X Q, Zhang H, Li S F, Wang Q G, Jian H Y, Yan B, Huang X Q. Study on genetic diversity of some Rosa germplsm in Yunnan bansed on SSR markers. Acta Horticulturae Sinica. 2008, 35(8):1227-1232. (in Chinese)

[3]徐宗大, 赵兰勇, 张  玲, 杨志莹. 玫瑰SRAP遗传多样性分析与品种指纹图谱构建. 中国农业科学, 2011, 44(8): 1662-1669.

Xu Z D, Zhao L Y, Zhang L, Yang Z Y. Analysis of genetic diversity and construction of fingerprint of Rosa rugosa by SRAP. Scientia Agricultura Sinica, 2011, 44(8): 1662-1669. (in Chinese)

[4]Chang Y K, Veilleux R E. Analysis of genetic diversity variability among Phalaenopsis species and hybrids using amplified fragment length polymorphism. Journal of American Society for Horticultural Science, 2009, 134(1):58-66.

[5]Ranjan P, Bhat K V, Misra R L, Singh S K, Ranjan J K. Genetic relationships of gladiolus cultivars inferred from fluorescence based AFLP markers. Scientia Horticulturae, 2010, 123:562-567.

[6]曾  丽, 赵粱军, 孙  佳, 赵子刚, 杨  帆. 万寿菊属品种资源遗传关系的ISSR分析. 中国农业科学, 2010, 43(1): 215-222.

Zeng L, Zhao L J, Sun J, Zhao Z G, Yang F. Analysis of genetic relatedness of genetic resources of Tagetes as revealed by ISSR. Scientia Agricultura Sinica, 2010, 43(1): 215-222. (in Chinese)

[7]张俊卫, 毛庆山, 包满珠. 梅遗传多样性的 SRAP 分析. 园艺学报, 2011, 38(1):117-124.

Zhang J W, Mao Q S, and Bao M Z. Analysis of genetic diversity among germplasm of Prunus mume Sieb. et Zucc. using SRAP markers. Acta Horticulturae Sinica, 2011, 38(1):117-124. (in Chinese)

[8]Barfuss M H J, Samuel R, Till W, Stuessy T F. Phylogenetic relationships in subfamily Tillandsioideae (Bromeliaceae) based on DNA sequence data from seven plastid regions. American Journal of Botany, 2005, 92(2):337-351.

[9]Barbará T, Martinelli G, Palma-Silva C, Fay M F, Mayo S, Lexer C. Genetic ralationships and variation in reproductive strategies in four closely related bromeliads adapted to neotropical ‘inselbergs’: Alcantarea glaziouana, A. regina, A. geniculata and A. imperialis (Bromeliaceae). Annals of Botany, 2009, 103:65-77.

[10]Duval M F, Buso G S C, Ferreira F R, Noyer J L, Coppens d’Eeckenbrugge G, Hamon P, and Ferreira M E. Relationships in Ananas and other related genera using chloroplast DNA restriction site variation. Genome, 2003, 46(6):990-1004.

[11]Palma-Silva C, Lexer C, Paggi G M, Barbará T, Bered F, Bodanese-Zanettini M H. Range-wide patterns of nuclear and chloroplast DNA diversity in Vriesea gigantea (Bromeliaceae), a neotropical forest species. Heredity, 2009, 103:503-512.

[12]李  萍, 石金磊, 胡永红, 陈  醇, 蒋昌华, 王  佳, 明  凤. 凤梨亚科光萼荷属与其近源属亲缘关系的ISSR分子鉴定. 种子, 2007, 26(11): 35-40.

Li P, Shi J L, Hu Y H, Chen C, Jiang C H, Wang J, Ming F. Moleculer identification of Aechmea and relative genera in Bromeliaceae by ISSR. Seed, 2007, 26(11): 35 - 40. (in Chinese)

[13]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 1980, 8:4321-4325.

[14]Yeh F C, Yang R, Boyle T. Microsoft Window-based Freeware for Population Genetic Analysis Popgene, version 1.31. Canada: University of Alberta, 1999.

[15]Rohlf F J. NTSYS pc: Numerical taxonomy and multivariate analysis system, Version 2.0. Exeter Software, New York. 2000.

[16]刘新龙, 马  丽, 陈学宽, 应雄美, 蔡  青, 刘家勇, 吴才文.云南甘蔗自育品种DNA指纹身份证构建. 作物学报, 2010, 36(2): 202-210.

Liu X L, Ma L, Chen X K, Ying X M, Cai Q, Liu J Y, Wu C W. Establishment of DNA fingerprint ID in Sugarcane cultivars in Yunnan. Acta Agronomica Sinica, 2010, 36(2):202-210. (in Chinese)

[17]张鲜艳, 张  飞, 陈发棣, 郭慧敏, 陈素梅. 12份不同地理居群野菊的遗传多样性分析. 南京农业大学学报, 2011, 34(3):48-54.

Zhang X Y, Zhang F, Chen F D, Guo H M, Chen S M. Analysis of genetic diversity among 12 geographical populations of Dendranthema indicum. Journal of Nanjing Agricultural University, 2011, 34(3):48-54. (in Chinese)

[18]缪恒彬, 陈发棣, 赵宏波. 85个大菊品种遗传关系的ISSR分析. 园艺学报, 2007, 34(5): 1243-1248.

Miao H B, Chen F D, Zhao H B. Genetic relationship of 85 chrysanthemum [Dendranthema×grandiflora  (Ramat.) Kitamura] cultivars revealed by ISSR analysis. Acta Horticulturae Sinica, 2007, 34(5): 1243-1248. (in Chinese)

[19]缪恒彬, 陈发棣, 赵宏波, 房伟民, 石丽敏. 应用 ISSR 对25 个小菊品种进行遗传多样性分析及指纹图谱构建. 中国农业科学, 2008, 41(11): 3735-3740.

Miao H B, Chen F D, Zhao H B, Fang W M, Shi L M. Genetic diversity and construction of fingerprinting of Chrysanthemum cultivars by ISSR markers. Scientia Agricultura Sinica, 2008, 41(11): 3735-3740. (in Chinese)

[20]Paggi G M, Palma-Silva C, Silveira L C T, Kaltchuk-Santos E, Bodanese-Zanettini M H, Bered F. Fertility of Vriesea gigantea Gaud. (Bromeliaceae) in southern Brazil. American Journal of Botany, 2007, 94(4):683-689.

[21]胡松华. 观赏凤梨. 北京: 中国林业出版社, 2003.

Hu S H. Ornamental Bromeliads. Beijing: China Forestry Publishing House, 2003. (in Chinese)

[22]邓科君, 张  勇, 鄢  丹, 彭金华, 张  硕, 任正隆. 鼠尾草属部分物种AFLP指纹图谱构建及遗传多样性分析. 植物遗传资源学报, 2010, 11(4): 483-490.

Deng K J, Zhang Y, Yan D, Peng J H, Zhang S, Ren Z L. Construction of AFLP fingerprint and genetic diversity analysis of the genus Slvia. Journal of Plant Genetic Resources, 2010, 11(4): 483-490. (in Chinese)

[23]匡  猛, 杨伟华, 许红霞, 王延琴, 周大云, 冯新爱. 中国棉花主栽品种DNA指纹图谱构建及SSR标记遗传多样性分析. 中国农业科学, 2011, 44(1):20-27.

Kuang M, Yang W H, Xu H X, Wang Y Q, Zhou D Y, Feng X A. Construction of DNA fingerprinting and analysis of genetic diversity with SSR markers for cotton major cultivars in China. Scientia Agricultura Sinica, 2011, 44(1):20-27. (in Chinese)

[24]段艳凤, 刘  杰, 卞春松, 段绍光, 徐建飞, 金黎平. 中国88个马铃薯审定品种SSR指纹图谱构建与遗传多样性分析. 作物学报, 2009, 35(8):1451-1457.

Duan Y F, Liu J, Bian C S, Duan S G, Xu J F, Jin L P. Construction  of fingerprinting and analysis of genetic diversity with SSR   markers for eighty-eight approved potato cultivars (Solanum tuberosum L.) in China. Acta Agronomica Sinica, 2009, 35(8): 1451-1457. (in Chinese)

[25]郑海燕, 粟建光, 戴志刚, 李  燕, 陈基权, 龚友才. 利用 ISSR 和RAPD 标记构建红麻种质资源分子身份证. 中国农业科学, 2010, 43(17): 3499-3510. 

Zheng H Y, Su J G, Dai Z G, Li Y, Chen J Q, Gong Y C. Establishment of molecular identity for kenaf germplasm using ISSR and RAPD markers. Scientia Agricultura Sinica, 2010, 43(17): 3499-3510. (in Chinese)

[26]童和林, 冯素萍, 贺军虎, 王静毅, 陈业渊, 孙光明, 于飞, 武耀 廷. 菠萝微卫星指纹图谱的构建. 果树学报, 2011, 28(2):240-245.

Tong H L, Feng S P, He J H, Wang J Y, Chen Y Y, Sun G M, Yu F, Wu Y T. Establishment of fingerprinting for pineapple (Ananas comosus) by SSR marker. Journal of Fruit Science, 2011, 28(2): 240-245. (in Chinese)
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