在白色杜洛克×二花脸资源家系中定位影响猪肉滴水损失的QTL

doi:10.3864/j.issn.0578-1752.2011.10.018

Abstract

Abstract: 【Objective】 A genome-wide scanning was performed to detect quantitative trait loci (QTL) for drip loss. 【Method】Using EZ-DripLoss and bag methods, drip loss of longissimus muscle (LM) and semimembranous muscle (SM) were measured at 24 and 48 h after sampling in 884 F2 animals from a White Duroc×Erhualian resource population. The correlations among 6 drip loss traits and between drip loss and other meat quality traits were analyzed by SAS version 9. All pigs in this experimental population were genotyped for 194 informative markers covering the entire porcine genome. QTL were identified by regression interval mapping using the online software of QTL Express. 【Result】 Correlation between the drip loss of two muscles or between drip loss obtained by two methods were high (r= 0.50-0.58, P＜0.01) , and the correlation between consecutive measurements was higher (r=0.59-0.72, P＜0.01). Drip loss was correlated with pH24h, Minolta L, subjective color score, marbling, moisture content and intramuscular fat content at moderate or low levels (r=0.09-0.35, P＜0.05). A total of 9 QTLs were identified, including 6 QTLs for drip loss of LM on SSC2, SSC10 and SSC12; the one on SSC10 reached the 5% genome-wide significant level. Three QTLs for drip loss of SM were detected on SSC2, SSC6 and SSC17. 【Conclusion】 Four novel QTL on SSC6, SSC10, SSC12 and SSC17 were identified. Several QTL for drip loss overlapped with previously mapped QTL for pH or intramuscular fat content. None of QTL affected drip loss of both LM and SM. Erhualian founders carried favorable alleles (decreasing drip loss) at most of the detected QTL.

Key words: pig, longissimus muscle, semimebraneous muscle, drip loss, QTL mapping

ZHOU Li-Hua, GUO Yuan-Mei, DUAN Yan-Yu, ZHANG Zhi-Yan, YANG Kai-Xuan, MA Jun-Wu. QTL Mapping for Drip Loss in a White Duroc × Erhualian F2 Resource Population[J].Scientia Agricultura Sinica, 2011, 44(10): 2131-2138.

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QTL Mapping for Drip Loss in a White Duroc × Erhualian F2 Resource Population

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