Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (23): 4911-4917.doi: 10.3864/j.issn.0578-1752.2011.23.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Polymorphism Analysis of csd in Five Populations of Chinese Honeybee

 LIU  Zhi-Yong, WANG  Zi-Long, WANG  Huan, ZENG  Zhi-Jiang   

  1. 1.江西农业大学蜜蜂研究所, 南昌 330045
    2.江西省职业病防治研究院实验动物中心，南昌 330006
  • Received:2010-12-27 Online:2011-12-01 Published:2011-02-23

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Abstract: 【Objective】 The objective of this study is to analyze the polymorphism of csd in different geographical populations of Chinese honeybee (Apis cerana cerana) (Hymenoptera: Apidae). 【Method】 Worker bees collected from Changbaishan (Jilin), Haikou (Hainan), Nanning (Guangxi), Shennongjia (Hubei) and Jing’an (Jiangxi) were used as materials, and genome DNA was extracted from each sample for PCR amplification of the csd region 3, PCR products were cloned and sequenced, polymorphism of csd in different geographical populations were analyzed by using the obtained sequences. 【Result】 The genomic region 3 of csd in Apis cerana cerana from 5 geographical populations were cloned and 32 csd haplotypes were obtained. The polymorphism of csd in Changbaishan (Jilin) and Jing’an (Jiangxi) populations were significantly higher than those in Nanning, Haikou and Shennongjia populations, while the polymorphism of csd between Changbaishan (Jilin) and Jing’an (Jiangxi) populations, and that between Nanning (Guangxi), Haikou (Hainan) and Shennongjia (Hubei) populations showed no significant difference. Meanwhile, population analysis based on csd haplotypes showed that the nucleotide divergence and genetic distance between Changbaishan (Jilin) and Jing’an (Jiangxi) populations were maximal, while those between Shennongjia (Hubei) and Nanning (Guangxi) populations were minimal. The phylogenetic analysis showed that all the haplotypes from these five geographical populations were mixed on the phylogenetic tree, not from 5 clade according to different geographical origins. 【Conclusion】 csd has a high level of polymorphism in all these populations, moreover, the polymorphism level showed some difference among populations.

Key words: Apis cerana cerana, csd, polymorphism, haplotype

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