Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (17): 3634-3640.doi: 10.3864/j.issn.0578-1752.2011.17.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effect of Exogenous Melatonin (MT) in Bovine Oocyte in vitro Maturation

GAO  Chao, TIAN  Xiu-Zhi, ZHANG  Lu, XU  Jing, WANG  Feng, ZHUO  Zhi-Yong, DAI  Yun-Ping, LIU  Guo-Shi   

  1. 中国农业大学动物科技学院
  • Received:2011-05-06 Revised:2011-06-27 Online:2011-09-01 Published:2011-07-29

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Abstract: 【Objective】 To optimize the bovine embryo in vitro production system，the effect of exogenous melatonin (MT) on improvement of bovine oocyte in vitro maturation was studied. 【Method】 Immature oocytes were cultured in vitro inmaturation medium supplemented with different concentrations of melatonin, then IVF statistical analysis of embryo cleavage rate, blastocyst rate and blastocyst cell number was conducted. Hormone levels were detected by radioimmunoassay in follicular fluid and the maturation medium and the the oocyte viability and reactive oxygen (ROS) levels were determined.【Result】MT existed in bovine follicular fluid and similar with serum. In 10-11 mol•L-1 treatment, the progesterone (P) levels (2.01±0.33) ng•mL-1 (P＜0.05) of maturation medium was significantly higher than that of controls (0.87±0.10) ng•mL-1 (P＜0.05). Compared with the control group (67.32±7.30)%, cleavage rate in 10-9 mol•L-1MT treatment (77.81±7.06)% was significantly increased and the blastocyst rate and cell number of blastocyst (29.61±9.55)%, (90.30±28.74) were significantly higher than that of controls (19.64±9.22)%, (75.07±25.98)  (P＜0.05). 10-9 mol•L-1 MT treatment improved the oocyte viability and decreased the ROS level (P＞0.05). 【Conclusion】 Melatonin exists in bovine follicular fluid and exogenous melatonin at a proper concentration can improve the in vitro maturation of bovine oocytes.

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