Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (16): 3368-3376.doi: 10.3864/j.issn.0578-1752.2011.16.009

• HORTICULTURE • Previous Articles     Next Articles

Construction and Analysis of Eggplant Thermo-Sensitive Parthenocarpy SSH cDNA Library

ZHANG  Ying, CHEN  Yu-Hui, ZHANG  Zhen-Xian, FANG  Zhi-Yuan, LIAN  Yong, LIU  Fu-Zhong   

  1. 1. 中国农业大学农学与生物技术学院
    2. 中国农业科学院蔬菜花卉研究所
  • Received:2011-01-17 Revised:2011-05-18 Online:2011-08-15 Published:2011-05-18

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Abstract: 【Objective】The aim of this experiment is to investigate the parthenocarpy-related genes in eggplant, and study the parthenocarpic molecular mechanism. 【Method】One forward and one reverse cDNA library were constructed by suppression subtractive hybridization (SSH). All the materials are from the same facultative parthenocapic line D-10, including the parhtenocarpic ovaries and fruits at low temperature, and the unparthenocarpic ovaries and fruits at optimal temperature. The positive clones of the libraries were sequenced randomly, analysed by BLAST， classified by GO and analysed by real-time quantitative PCR. 【Result】A total of 2 347 positive clones in the forward and reverse libraries were obtained. After sequenced and assembled, 1 248 high-quality unigenes were gotten. Blast analysis was made with no redundant database, 1 109 of these unigenes were homologous to known genes, and 139 could be new genes. In Gene Ontology database, 527 unigenes were assigned functional description, and 206, 445, 361 ESTs were, respectively, involved in cell component, molecular function and biological process. According to classification results, the difference between parthenocarpy and unparthenocarpy almost existed in intracellular region. Parthenocarpy may be regulated by some factors and kinases, and most of the differentially expressed genes were involved in basic biological processes.【Conclusion】The SSH cDNA libraries were successfully constructed. Three parthenocarpic related unigenes, including MADS-box gene, pistil extensin-like protein and fruit-ripening gene, were obtained and the possible mechanism was analyzed.

Key words: eggplant, parthenocarpy, SSH, EST

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