Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (3): 651-656 .doi: 10.3864/j.issn.0578-1752.sas-2010-06139

• RESEARCH NOTES • Previous Articles    

Study on the Genetic Diversity of Some Camellia japonica (Naidong) Cultivars Based on AFLP Markers

WANG Kui-ling,MU Shao-hua,LIU Qing-chao,TANG Qi-he,LIU Qing-hua
   

  1. (青岛农业大学园林园艺学院)
  • Received:2010-01-22 Revised:2010-10-08 Online:2011-02-01 Published:2011-02-01
  • Contact: LIU Qing-hua

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Abstract:

【Objective】The genetic diversity of Camellia Japonica (Naidong) cultivar accessions was analyzed at molecular level,and the helpful information for cultivar classification was obtained.【Method】The genetic diversity of 16 cultivars and 2 mutation types of Camellia Japonica (Naidong) with 5 cultivars of C. japonica from south of China, 2 cultivars of C. reticulata was analyzed by the fluorescent-AFLP marker techniques with 8 pairs of EcoRⅠ/Mse 1 primer combinations. 【Result】 In the genomes of the 30 tested samples,a total of 1 062 spectral bands amplified from the 8 pairs of primer combinations were selected from AFLP analysis and 987 bands appeared to be polymorphic (92.9%). The data were used to calculate the Nei’s genetic distance and to construct a dendrogram based on UPGMA cluster analysis. 【Conclusion】 It showed that the cultivars of Camellia japonica (Naidong) have a remote genetic relationship compared with the cultivars of Camellia from south of China,and cultivars of Camellia japonica (Naidong) can be divided into 6 groups,which indicated that Camellia japonica (Naidong) have rich genetic variability.

Key words: Camellia japonica (Naidong), AFLP marker, genetic diversity, genetic relationship

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