Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (12): 2446-2450 .doi: 10.3864/j.issn.0578-1752.at-2005-5547

• PLANT PROTECTION • Previous Articles     Next Articles

HCD in Tobacco Induced by HarpinXoo Showing Characteristics of PCD

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  • Received:2005-05-30 Revised:1900-01-01 Online:2005-12-10 Published:2005-12-10

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Abstract: The proteinaceous elicitor harpinXoo from pathogenic bacteria Xanthomonas oryzae pv. oryzae was found to elicit lesions reminiscent of the hypersensitive response (HR) in tobacco plants (Nicotiana tobacum L., Xanthi; NC). Tobacco leaves infiltrated with 40 μg·ml-1 harpinXoo exhibited a distinct hypersensitive cell death after 20 hours of infiltration, but micro-HR could be observed at the 8th hour of infiltration by Trypan blue staining. Chromatin condensation and nuclear DNA fragmentation, the features of programmed cell death (PCD), were found in the treated leaves using DAPI staining and agarose gel analysis respectively. These results indicated that tobacco leaves induced by harpinXoo had the properties of PCD. In addition, analysis of the mRNA accumulation of hin1 and hsr203J, two marker genes of HCD, indicated a correlation between genes expression and tobacco cell death induced by harpinXoo。

Key words: harpinXoo, HCD, chromatin condensation, DNA fragmentation, gene expression

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