草莓连作对土壤微生物及碳氮磷功能基因丰度的影响

doi:10.3864/j.issn.0578-1752.2025.17.010

Abstract

Abstract:

【Objective】This paper aimed to investigate the characteristic of soil microorganisms and soil function under long-term strawberry continuous cropping and to clarify the effects of long-term strawberry continuous cropping on soil bacterial and fungal community structure and carbon, nitrogen and phosphorus metabolism gene abundance, so as to provide the scientific basis for improving the soil microecological balance and soil function of continuous cropping in the future.【Method】The real-time PCR, Miseq sequencing and high-throughput chip technologies were applied to determine soil bacteria, fungi and function under strawberry cultivated for 1, 3 and 10 year.【Result】The strawberry continuous cropping reduced the soil pH, but increased the soil nutrient content, in which the soil organic matter content increased from 21.2 g·kg^-1to 32.4 g·kg^-1. The bacterial abundance in rhizosphere and bulk soil was increased and then decreased as the years of cultivation. The abundance of bulk soil fungi was similar to the trend of bacteria, but its abundance was significantly reduced in the rhizosphere, indicating that bacteria and fungi response differently to continuous cropping. Continuous cropping had no significant effect on bacterial diversity, but significantly reduced fungal diversity and significantly changed soil microbial composition. Based on UniFrac distance, it was found that the fungal community UniFrac distance (0.64-1.36) was much higher than the bacteria (0.028-0.111), indicating that the influence of continuous cropping on fungal community structure was higher than that of bacteria. Correlation analysis showed that bacterial community structure was significantly correlated with soil pH, while fungal community structure was significantly correlated with soil nutrient status (such as soil available P, alkali-hydrolysable, and soil organic matter). Long-term continuous cropping of strawberry changed the metabolic function gene abundance of soil carbon, nitrogen and phosphorus, which significantly reduced the soil carbon fixation gene accA, while the nitrogen fixation gene nifH and phosphorus metabolism related functions (phoD, phoX and pqqC genes) first increased and then decreased. The partial least squares path model (PLS-PM) analysis showed that the fungal community structure (abundance, diversity and composition) caused by long-term strawberry continuous cropping had a higher impact on soil carbon, nitrogen and phosphorus metabolism gene abundance than the bacterial community structure.【Conclusion】This study showed that soil function gene abundance changed caused by long-term strawberry continuous cropping was mainly caused by the changes of fungal community structure. Thus, the soil fungal community structure should be regulated to improve the health status of long-term continuous cropping soil.

Key words: strawberry, continuous cropping, community composition, bacteria and fungi, soil microbial functional genes

WANG QingFeng, CHU ChangBin, ZHAO Zheng, WU ShuHang, ZHOU DePing. Effects of Strawberry Continuous Cropping on Soil Microbial Community Composition and Carbon, Nitrogen and Phosphorus Metabolism Gene Abundance[J].Scientia Agricultura Sinica, 2025, 58(17): 3488-3502.

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References 49

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处理 Treatment	酸碱度 pH	电导率 EC (μs·cm^-1)	速效钾 Available K (mg·kg^-1)	有效磷 Available P (mg·kg^-1)	有机质 Organic matter (g·kg^-1)	碱解氮 Alkali-hydrolysable N (mg·kg^-1)
T1	7.47±0.02a	497.3±1.5c	826.7±11.6b	45.97±0.54c	21.2±0.02b	150.5±7.0b
T3	7.43±0.02b	520.0±1.7a	1301.7±2.9a	210.01±0.41a	32.4±0.00a	214.7±8.1a
T10	7.16±0.01c	514.0±1.0b	821.7±2.9b	79.2±0.24b	32.3±0.01a	208.8±16.5a

组别Groups	处理Treatment	ACE指数	Chao指数	Shannon指数	Sobs指数
细菌 Bacteria	RT1	1950±158a	1949±158a	7.06±0.10a	1946±159a
	RT3	2020±49a	2020±50a	7.09±0.01a	2016±49a
	RT10	2022±58a	2022±59a	7.00±0.01a	2015±55a
	T1	1939±187a	1938±186a	7.05±0.11a	1934±186a
	T3	1919±84a	1919±83a	7.06±0.06a	1916±86a
	T10	2120±19a	2119±18a	7.07±0.05a	2116±17a
真菌 Fungi	RT1	553±1b	553±1b	4.82±0.05ab	552±2b
	RT3	482±18c	481±18c	4.56±0.06b	481±18c
	RT10	306±10d	306±9d	3.73±0.14c	305±11d
	T1	615±6a	614±6a	4.93±0.03a	614±7a
	T3	509±14c	509±14c	3.97±0.15c	507±14c
	T10	337±17d	337±17d	3.70±0.16c	337±17d

Effects of Strawberry Continuous Cropping on Soil Microbial Community Composition and Carbon, Nitrogen and Phosphorus Metabolism Gene Abundance

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