Scientia Agricultura Sinica ›› 2018, Vol. 51 ›› Issue (17): 3315-3324.doi: 10.3864/j.issn.0578-1752.2018.17.007

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Influence of Long-Term Nitrogen and Phosphorus Fertilization on Arbuscular Mycorrhizal Fungi Community in Mollisols of Northeast China

WANG QingFeng1,2, JIANG Xin1,2, MA MingChao1,2, GUAN DaWei1,2, ZHAO BaiSuo2, WEI Dan3, CAO FengMing1,2, LI Li1,2, LI Jun1,2   

  1. 1 Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081; 2 ＆Safety Risk Assessment for Microbial Products (Beijing), Ministry of Agriculture, Beijing 100081; 3 Institute of Soil Fertility and Environmental Sources, Heilongjiang Academy of Agricultural Sciences, Harbin 150086Laboratory of Quality
  • Received:2017-12-14 Online:2018-09-01 Published:2018-09-01

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Abstract: 【Objective】Arbuscular mycorrhizal (AM) fungi, which form symbiotic relationships with the majority of land plants, can provide plants with critical nutrients, act as protectants against phytopathogens, and help plants withstand stresses. In order to clarify the effects of different doses of nitrogen (N) and phosphorus (P) fertilization on the community composition and reveal the main driving factors of AM fungi, a long-term fertilizer experiment (37-year) was set up in mollisols of northeast China. This study would provide evidence for further enhancing fertilization and using AM fungi to increase availability of soil nutrients for plants.【Method】Base on a long-term fertilization field experiment, Illumina MiSeq platform were used to analyze the effects of different N and P fertilization on AM fungal community. The soil samples were collected and analyzed from five fertilization regimes: No fertilizer (CK), normal N fertilizer (N1), normal N plus normal P fertilizers (N1P1), duple N fertilizer (N2), and duple N plus duple P fertilizers (N2P2). A correlation analysis was used to reveal the main important factors for determining the AM fungal community composition. 【Result】 Long-term N and P fertilization decreased soil pH and available K, however, which increased total N, KCl-extractable NO3- and NH4+, and organic matter. N-fertilization (N1 and N2) did not significantly change soil AMF diversity (P＞0.05), while N- plus P-fertilization (N1P1 and N2P2) decreased it compared with CK (P＜0.05). Glomeraceae was the most abundant family in soils, accounting for 45.5% of all AM fungi. In genus level, all fertilization decreased the relative abundance of Funneliformis and Septoglomus, whereas increased Paraglomus;N- plus P-fertilization increased the relative abundance of Glomus and Funneliformis, but decreased Gigaspora and Paraglomus. Nonmetric Multidimensional Scaling analyzed result showed that the community composition in no fertilization, N fertilization, and N- plus P-fertilization was significantly different with each other. And a redundancy analysis indicated that soil pH, concentration of available P were the main environment factors (P＜0.05) affecting the AM fungal community variation. 【Conclusion】 Our research demonstrated that long-term fertilization changed soil AM fungal community composition in mollisols. N-fertilization did not change AM fungal diversity while N- plus P-fertilization decreased it. It was concluded that soil pH and available P concentration were the main factors affecting AM fungal community variation.

Key words: long-term fertilization, mollisols, AMF, microbial community composition, Illumina Miseq

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