桃生长势相关基因PpSAUR73功能鉴定

doi:10.3864/j.issn.0578-1752.2023.20.012

Abstract

Abstract:

【Objective】 The object of this study was to isolate a peach potential-related gene PpSAUR73, to analyze its expression response to hormones, and to identify its role in regulating seedling growth in transgenic Arabidopsis, so as to provide the molecular basis for the regulation of tree potential. 【Method】 Using Zhongyou Pan 9 as the material for hormone treatment, the real-time fluorescence quantitative analysis was used to analyze the dynamic response of PpSAUR73 within 24 hours. PpSAUR73 was cloned from the peach variety Jiuyan. PpSAUR73 overexpression vector was constructed and transformed into Arabidopsis. Phenotypic observation of genetical modified Arabidopsis was carried out, and the germination rate statistics of both genetically modified and wild-type Arabidopsis sown simultaneously were performed too. The root length and hypocotyl of 7-day growing Arabidopsis with consistent germination were measured, and Arabidopsis with consistent germination was treated with different concentration hormone. Transcriptome sequencing was performed using 7-day-old seedlings, and the differentially expressed genes were analyzed by functional analysis, KEGG pathway enrichment analysis, and regulatory genes analysis, respectively. 【Result】 PpSAUR73 could respond quickly to hormone treatments. The overexpression of PpSAUR73 could affect the germination of Arabidopsis seeds. The hypocotyl and root length of seedlings were longer than those of wild type. In addition, the rosette of transgenic Arabidopsis was larger, and the overall growth potential was larger than wild type. The transgenic Arabidopsis showed decreased sensitivity to auxin. The transcriptome analysis of overexpressing PpSAUR73 showed that there were 128 differentially expressed genes in both control groups, including 84 up-regulated genes and 44 down-regulated genes, and 20 differentially expressed genes were described. The GO function significant enrichment analysis of the differentially expressed genes generated by overexpression of PpSAUR73 showed that the differentially expressed genes were the most abundant in cell components, located in cytoplasm, cell membrane, organelle and extracellular regions. KEGG pathway enrichment analysis on differentially expressed genes were conducted, and the results showed that the differentially expressed genes in pairwise comparisons CK vs SAUR73-1 and CK vs SAUR73-14 were mainly enriched in phenylalanine biosynthesis pathway, plant hormone signal transduction pathway, starch sucrose metabolic pathway and other metabolic pathways. In the phenylalanine biosynthesis pathway, PpSAUR73 could regulate the upregulation of peroxidase encoding genes AT1G05260, AT3G01190, AT3G32980 and AT5G15180. Peroxidases were associated with lignin synthesis, and lignin content was significantly correlated with plant growth, suggesting that overexpression of PpSAUR73 might be involved in regulating lignin synthesis in Arabidopsis and thus growth. In plant hormone signal transduction pathway, the expression of some auxin responsive genes of AtSAUR41, AtSAUR71, AtSAUR51, AtSAUR72 and AtSAUR1 in auxin signal transduction pathway was down-regulated, the expression of phosphatase protein AtPP2CA in abscisic acid signal transduction pathway was up-regulated, and the expression of abscisic acid signal pathway gene AtPYL5 was down-regulated. PpSAUR73 could regulate the growth of Arabidopsis and participate in multiple hormone signal transduction pathways. 【Conclusion】 This study found that PpSAUR73 could quickly respond to hormones and regulate the growth in transgenic Arabidopsis. The differentially expressed genes caused by overexpressed genes caused by overexpression of PpSAUR73 were mainly enriched in metabolic pathways, such as phenylalanine biosynthesis pathway, plant hormone signaling pathway, and starch sucrose metabolism pathway. PpSAUR73 also played an important role in IAA and ABA signal transduction pathways, it was speculated that it played an important role in the growth and development of peach trees.

Key words: peach, PpSAUR73, gene expression, hormone treatment, signal transduction

YANG Li, CAO HongBo, ZHANG XueYing, ZHAI HanHan, LI XinMiao, PENG JiaWei, TIAN Yi, CHEN HaiJiang. Functional Identification of Peach Gene PpSAUR73[J].Scientia Agricultura Sinica, 2023, 56(20): 4072-4086.

Figures/Tables 13

Table 1

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Table 2

Information description of some common differentially expressed genes in SAUR73"

基因ID Gene ID	描述 Description	KEGG通路 KEGG pathway
AT1G23720	未命名蛋白物质 Unnamed protein product
AT1G43790	气管元件分化相关6 Tracheary element differentiation-related 6
AT1G43800	植物硬脂酰酰基载体蛋白去饱和酶家族蛋白 Plant stearoyl-acyl-carrier-protein desaturase family protein	脂肪酸生物合成（ko00061），生物合成不饱和脂肪酸（ko01040），脂肪酸代谢（ko01212） Fatty acid biosynthesis (ko00061), Biosynthesis of unsaturated fatty acids (ko01040), Fatty acid metabolism (ko01212)
AT1G47395	假设蛋白质AT1G47395 Hypothetical protein AT1G47395
AT1G56600	半乳糖醇合成酶 Galactinol synthase 2	半乳糖代谢（ko00052） Galactose metabolism (ko00052)
AT1G60190	ARM重复超家族蛋白 ARM repeat superfamily protein	泛素介导的蛋白水解（ko04120） Ubiquitin mediated proteolysis (ko04120)
AT1G77120	乙醇脱氢酶1 Alcohol dehydrogenase 1	糖酵解/糖异生（ko00010），脂肪酸降解（ko00071），酪氨酸代谢（ko00350），亚麻酸代谢（ko00592） Glycolysis/Gluconeogenesis (ko00010), Fatty acid degradation (ko00071), Tyrosine metabolism (ko00350), alpha-Linolenic acid metabolism (ko00592)
AT3G12830	SAUR样生长素反应蛋白家族 SAUR-like auxin-responsive protein family	植物激素信号转导（ko04075） Plant hormone signal transduction (ko04075)
AT3G14440	9-顺式环氧类胡萝卜素双加氧酶 Nine-cis-epoxycarotenoid dioxygenase 3	类胡萝卜素生物合成（ko00906） Carotenoid biosynthesis (ko00906)
AT3G28550	富含脯氨酸的延伸蛋白家族蛋白 Proline-rich extensin-like family protein
AT3G43190	蔗糖合酶4 Sucrose synthase 4	淀粉和蔗糖代谢（ko00500） Starch and sucrose metabolism (ko00500)
AT4G33070	硫胺素焦磷酸依赖的丙酮酸脱羧酶家族蛋白 Thiamine pyrophosphate dependent pyruvate decarboxylase family protein	糖酵解/糖异生（ko00010） Glycolysis/Gluconeogenesis (ko00010)
AT5G04150	未知的，偏 Unknown, partial
AT5G35190	假设蛋白质AXX17_AT5G31360 Hypothetical protein AXX17_AT5G31360
ATCG00430	NADH脱氢酶亚基K NADH dehydrogenase subunit K	氧化磷酸化（ko00190） Oxidative phosphorylation (ko00190)
ATCG00470	ATP合成酶CF1 epsilon亚基 ATP synthase CF1 epsilon subunit	氧化磷酸化（ko00190），光合作用（ko00195） Oxidative phosphorylation (ko00190), Photosynthesis (ko00195)
ATCG00530	包膜蛋白 Envelope membrane protein	光合作用（ko00195） Photosynthesis (ko00195)
ATCG00670	ATP依赖性Clp蛋白酶蛋白水解亚基 ATP-dependent Clp protease proteolytic subunit
ATCG00810	核糖体蛋白L22 Ribosomal protein L22	核糖体（ko03010） Ribosome (ko03010)
ATCG01020	核糖体蛋白L32 Ribosomal protein L32	核糖体（ko03010） Ribosome (ko03010)

Table 2

Fig. 7

Fig. 8

Fig. 9

Table 3

Fig. 10

References 45

[1]	STORTENBEKER N, BEMER M. The SAUR gene family: The plant’s toolbox for adaptation of growth and development. Journal of Experimental Botany, 2019, 70(1): 17-27. doi: 10.1093/jxb/ery332
[2]	SPARTZ A K, LOR V S, REN H, OLSZEWSKI N E, MILLER N D, WU G S, SPALDING E P, GRAY W M. Constitutive expression of Arabidopsis SMALL AUXIN UP RNA19 (SAUR19) in tomato confers auxin-independent hypocotyl elongation. Plant Physiology, 2017, 173(2): 1453-1462. doi: 10.1104/pp.16.01514
[3]	BEMER M, VAN MOURIK H, MUIÑO J M, FERRÁNDIZ C, KAUFMANN K, ANGENENT G C. FRUITFULL controls SAUR10 expression and regulates Arabidopsis growth and architecture. Journal of Experimental Botany, 2017, 68(13): 3391-3403. doi: 10.1093/jxb/erx184
[4]	LI X H, LIU G Y, GENG Y H, WU M, PEI W F, ZHAI H H, ZANG X S, LI X L, ZHANG J F, YU S X, YU J W. A genome-wide analysis of the small auxin-up RNA (SAUR) gene family in cotton. BMC Genomics, 2017, 18(1): 815. doi: 10.1186/s12864-017-4224-2 pmid: 29061116
[5]	WU J, LIU S Y, HE Y J, GUAN X Y, ZHU X F, CHENG L, WANG J, LU G. Genome-wide analysis of SAUR gene family in Solanaceae species. Gene, 2012, 509(1): 38-50. doi: 10.1016/j.gene.2012.08.002 pmid: 22903030
[6]	CHAE K, ISAACS C G, REEVES P H, MALONEY G S, MUDAY G K, NAGPAL P, REED J W. Arabidopsis SMALL AUXIN UP RNA63 promotes hypocotyl and stamen filament elongation. The Plant Journal, 2012, 71(4): 684-697. doi: 10.1111/tpj.2012.71.issue-4
[7]	SPARTZ A K, REN H, PARK M Y, GRANDT K N, LEE S H, MURPHY A S, SUSSMAN M R, OVERVOORDE P J, GRAY W M. SAUR inhibition of PP2C-D phosphatases activates plasma membrane H⁺-ATPases to promote cell expansion in Arabidopsis. The Plant Cell, 2014, 26(5): 2129-2142. doi: 10.1105/tpc.114.126037
[8]	KONG Y Y, ZHU Y B, GAO C, SHE W J, LIN W Q, CHEN Y, HAN N, BIAN H W, ZHU M Y, WANG J H. Tissue-specific expression of SMALL AUXIN UP RNA41 differentially regulates cell expansion and root meristem patterning in Arabidopsis. Plant and Cell Physiology, 2013, 54(4): 609-621. doi: 10.1093/pcp/pct028
[9]	PARK J E, KIM Y S, YOON H K, PARK C M. Functional characterization of a small auxin-up RNA gene in apical hook development in Arabidopsis. Plant Science, 2007, 172(1): 150-157. doi: 10.1016/j.plantsci.2006.08.005
[10]	KANT S, BI Y M, ZHU T, ROTHSTEIN S J. SAUR39, a small auxin-up RNA gene, acts as a negative regulator of auxin synthesis and transport in rice. Plant Physiology, 2009, 151(2): 691-701. doi: 10.1104/pp.109.143875 pmid: 19700562
[11]	KODAIRA K S, QIN F, TRAN L S P, MARUYAMA K, KIDOKORO S, FUJITA Y, SHINOZAKI K, YAMAGUCHI-SHINOZAKI K. Arabidopsis Cys2/His2 zinc-finger proteins AZF₁ and AZF₂ negatively regulate abscisic acid-repressive and auxin-inducible genes under abiotic stress conditions. Plant Physiology, 2011, 157(2): 742-756. doi: 10.1104/pp.111.182683
[12]	FAVERO D S, LE K N, NEFF M M. Brassinosteroid signaling converges with SUPPRESSOR OF PHYTOCHROME B4-#3 to influence the expression of SMALL AUXIN UP RNA genes and hypocotyl growth. The Plant Journal, 2017, 89(6): 1133-1145. doi: 10.1111/tpj.13451 pmid: 27984677
[13]	HU W F, YAN H W, LUO S S, PAN F, WANG Y, XIANG Y. Genome-wide analysis of poplar SAUR gene family and expression profiles under cold, polyethylene glycol and indole-3-acetic acid treatments. Plant Physiology and Biochemistry, 2018, 128: 50-65. doi: S0981-9428(18)30179-7 pmid: 29758473
[14]	OH E, ZHU J Y, BAI M Y, ARENHART R A, SUN Y, WANG Z Y. Cell elongation is regulated through a central circuit of interacting transcription factors in the Arabidopsis hypocotyl. eLife, 2014, 3: e03031. doi: 10.7554/eLife.03031
[15]	VAN MOURIK H, VAN DIJK A D J, STORTENBEKER N, ANGENENT G C, BEMER M. Divergent regulation of Arabidopsis SAUR genes: a focus on the SAUR10-clade. BMC Plant Biology, 2017, 17(1): 1-14. doi: 10.1186/s12870-016-0951-9
[16]	翟宇杰. 不同品种桃树生长势的差异及相关基因的表达与调控[D]. 保定: 河北农业大学, 2020.
	ZHAI Y J. Difference of growth potential of different peach cultivars and expression and regulation of related genes[D]. Baoding: Hebei Agricultural University, 2020. (in Chinese)
[17]	周晓雅. 多效唑抑制桃新梢生长的效应及相关分子机理研究[D]. 保定: 河北农业大学, 2020.
	ZHOU X Y. Study on the effect of paclobutrazol on inhibiting the growth of peach shoots and its related molecular mechanism[D]. Baoding: Hebei Agricultural University, 2020. (in Chinese)
[18]	翟含含, 翟宇杰, 田义, 张叶, 杨丽, 温陟良, 陈海江. 桃SAUR家族基因分析及PpSAUR5功能鉴定. 园艺学报, 2023, 50(1): 1-14. doi: 10.16420/j.issn.0513-353x.2021-0932
	ZHAI H H, ZHAI Y J, TIAN Y, ZHANG Y, YANG L, WEN Z L, CHEN H J. Genome-wide identification of peach SAUR gene family and characterization of PpSAUR5 gene. Acta Horticulturae Sinica, 2023, 50(1): 1-14. (in Chinese)
[19]	王文然, 樊秀彩, 张文颖, 刘崇怀, 房经贵, 王晨. 果树赤霉素代谢与信号途径研究进展. 生物技术通报, 2017, 33(11): 1-7. doi: 10.13560/j.cnki.biotech.bull.1985.2017-0650
	WANG W R, FAN X C, ZHANG W Y, LIU C H, FANG J G, WANG C. Study progress on gibberellin metabolism and signaling transduction pathway in fruits trees. Biotech Bulletin, 2017, 33(11): 1-7. (in Chinese)
[20]	徐献斌, 耿晓月, 李慧, 孙丽娟, 郑焕, 陶建敏. 基于转录组分析ABA促进葡萄花青苷积累相关基因. 中国农业科学, 2022, 55(1): 134-151. doi: 10.3864/j.issn.0578-1752.2022.01.012.
	XU X B, GENG X Y, LI H, SUN L J, ZHENG H, TAO J M. Transcriptome analysis of genes involved in ABA-induced anthocyanin accumulation in grape. Scientia Agricultura Sinica, 2022, 55(1): 134-151. doi: 10.3864/j.issn.0578-1752.2022.01.012. (in Chinese)
[21]	WEI J H, SONG Y R. Recent advances in study of lignin biosynthesis and manipulation. Journal of Integrative Plant Biology, 2001, 43(8): 771-779.
[22]	ZENG Y, ZHAO T H, KERMODE A R. A conifer ABI₃-interacting protein plays important roles during key transitions of the plant life cycle. Plant Physiology, 2013, 161(1): 179-195. doi: 10.1104/pp.112.206946 pmid: 23144188
[23]	SWARUP R, PERRY P, HAGENBEEK D, VAN DER STRAETEN D, BEEMSTER G T S, SANDBERG G, BHALERAO R, LJUNG K, BENNETT M J. Ethylene upregulates auxin biosynthesis in Arabidopsis seedlings to enhance inhibition of root cell elongation. The Plant Cell, 2007, 19(7): 2186-2196. doi: 10.1105/tpc.107.052100
[24]	SPARTZ A K, LEE S H, WENGER J P, GONZALEZ N, ITOH H, INZÉ D, PEER W A, MURPHY A S, OVERVOORDE P J, GRAY W M. The saur19 subfamily of small auxin up RNA genes promote cell expansion. The Plant Journal, 2012, 70(6): 978-990. doi: 10.1111/j.1365-313X.2012.04946.x pmid: 22348445
[25]	LI Z G, CHEN H W, LI Q T, TAO J J, BIAN X H, MA B, ZHANG W K, CHEN S Y, ZHANG J S. Retraction Note: Three SAUR proteins SAUR76, SAUR77 and SAUR78 promote plant growth in Arabidopsis. Scientific Reports, 2022, 12: 1994. doi: 10.1038/s41598-022-06178-8
[26]	吴慧娟, 刘艳, 王锡锋. 小麦矮缩病毒引起的植株矮化与赤霉素代谢的相关性分析. 中国农业科学, 2017, 50(17): 3337-3343. doi: 10.3864/j.issn.0578-1752.2017.17.008.
	WU H J, LIU Y, WANG X F. Correlational analyses between dwarfing of plant height induced by wheat dwarf virus (WDV) infection and gibberellin metabolism. Scientia Agricultura Sinica, 2017, 50(17): 3337-3343. doi: 10.3864/j.issn.0578-1752.2017.17.008. (in Chinese)
[27]	LOCKARD R G, SCHNEIDER G W. Stock and scion growth relationships and the dwarfing mechanism in apple//Horticultural Reviews. Wiley Online Books, 1981: 315-375.
[28]	张志华, 刘新彩, 刘彦红, 高仪, 王红霞. 核桃幼树内源激素与生长势的关系. 林业科学, 2006, 42(9): 131-133.
	ZHANG Z H, LIU X C, LIU Y H, GAO Y, WANG H X. Study on relationship between hormone and growth vigor of young Juglans regia. Scientia Silvae Sinicae, 2006, 42(9): 131-133. (in Chinese)
[29]	闫艳华. 不同外源激素处理对曼陀罗种子萌发及幼苗生长的影响. 北方园艺, 2020(23): 115-122.
	YAN Y H. Effects of different exogenous hormones on seed germination and seedling growth of Datura stramonium. Northern Horticulture, 2020(23): 115-122. (in Chinese)
[30]	STAMM P, KUMAR P P. Auxin and gibberellin responsive Arabidopsis SMALL AUXIN UP RNA36 regulates hypocotyl elongation in the light. Plant Cell Reports, 2013, 32(6): 759-769. doi: 10.1007/s00299-013-1406-5
[31]	HARMER S L, BROOKS C J. Growth-mediated plant movements: hidden in plain sight. Current Opinion in Plant Biology, 2018, 41: 89-94. doi: S1369-5266(17)30094-8 pmid: 29107827
[32]	SHIN J H, MILA I, LIU M C, RODRIGUES M A, VERNOUX T, PIRRELLO J, BOUZAYEN M. The RIN-regulated Small Auxin-Up RNA SAUR69 is involved in the unripe-to-ripe phase transition of tomato fruit via enhancement of the sensitivity to ethylene. The New Phytologist, 2019, 222(2): 820-836. doi: 10.1111/nph.2019.222.issue-2
[33]	WANG J J, SUN N, ZHANG F F, YU R B, CHEN H D, DENG X W, WEI N. SAUR17 and SAUR50 differentially regulate PP2C-D1 during apical hook development and Cotyledon opening in Arabidopsis. The Plant Cell, 2020, 32(12): 3792-3811. doi: 10.1105/tpc.20.00283
[34]	HOU K, WU W, GAN S S. SAUR36, a small auxin up RNA gene, is involved in the promotion of leaf senescence in Arabidopsis. Plant Physiology, 2013, 161(2): 1002-1009. doi: 10.1104/pp.112.212787
[35]	余洪, 易倩, 张曼曼, 朱世平, 王福生, 赵晓春. CclSAUR49基因的表达特征及对类柠檬苦素生物合成的影响. 果树学报, 2021, 38(8): 1240-1251.
	YU H, YI Q, ZHANG M M, ZHU S P, WANG F S, ZHAO X C. Characteristics of CclSAUR49 expression and its effect on limonoids bio-synthesis in citrus. Journal of Fruit Science, 2021, 38(8): 1240-1251. (in Chinese)
[36]	REN H, GRAY W. SAUR proteins as effectors of hormonal and environmental signals in plant growth. Molecular Plant, 2015, 8(8): 1153-1164. doi: 10.1016/j.molp.2015.05.003 pmid: 25983207
[37]	KANT S, PENG M S, ROTHSTEIN S J. Genetic regulation by NLA and microRNA827 for maintaining nitrate-dependent phosphate homeostasis in Arabidopsis. PLoS Genetics, 2011, 7(3): e1002021. doi: 10.1371/journal.pgen.1002021
[38]	李伟. 桤木苗期不同无性系PAL和木质素及生长关系的研究[D]. 北京: 中国林业科学研究院, 2001.
	LI W. Study on the relationship between PAL and lignin and growth of different clones of Alnus cremastogyne at seedling stage[D]. Beijing: Chinese Academy of Forestry, 2001. (in Chinese)
[39]	佘恒志, 聂姣, 李英双, 刘星贝, 胡丹, 马珊, 次仁卓嘎, 汪灿, 吴东倩, 阮仁武, 易泽林. 不同抗倒伏能力甜荞品种茎秆木质素及其单体合成特征. 中国农业科学, 2017, 50(7): 1202-1209. doi: 10.3864/j.issn.0578-1752.2017.07.003.
	SHE H Z, NIE J, LI Y S, LIU X B, HU D, MA S, CIRENZHUOGA, WANG C, WU D Q, RUAN R W, YI Z L. Lignin and lignin monomer synthetic characteristics of culm in common buckwheat with different lodging resistance capabilities. Scientia Agricultura Sinica, 2017, 50(7): 1202-1209. doi: 10.3864/j.issn.0578-1752.2017.07.003. (in Chinese)
[40]	郭光艳, 柏峰, 刘伟, 秘彩莉. 转录因子对木质素生物合成调控的研究进展. 中国农业科学, 2015, 48(7): 1277-1287. doi: 10.3864/ j.issn.0578-1752.2015.07.03.
	GUO G Y, BAI F, LIU W, BI C L. Advances in research of the regulation of transcription factors of lignin biosynthesis. Scientia Agricultura Sinica, 2015, 48(7): 1277-1287. doi: 10.3864/j.issn.0578- 1752.2015.07.03. (in Chinese)
[41]	胡丹, 刘星贝, 汪灿, 杨浩, 李鹤鑫, 阮仁武, 袁晓辉, 易泽林. 不同抗倒性甜荞茎秆木质素合成关键酶基因的表达分析. 中国农业科学, 2015, 48(9): 1864-1872. doi: 10.3864/j.issn.0578-1752.2015. 09.20.
	HU D, LIU X B, WANG C, YANG H, LI H X, RUAN R W, YUAN X H, YI Z L. Expression analysis of key enzyme genes in lignin synthesis of culm among different lodging resistances of common buckwheat (Fagopyrum esculentum moench). Scientia Agricultura Sinica, 2015, 48(9): 1864-1872. doi: 10.3864/j.issn.0578-1752.2015. 09.20. (in Chinese)
[42]	吕婷雯, 沈汝波, 杨洪强, 范伟国, 张瑞雪, 王利, 徐颖, 曹辉, 宁留芳, 周春然. 施加有机肥下木质素对平邑甜茶根系活力及根际土壤微生态的影响. 山东农业大学学报(自然科学版), 2018, 49(4): 561-565.
	LÜ T W, SHEN R B, YANG H Q, FAN W G, ZHANG R X, WANG L, XU Y, CAO H, NING L F, ZHOU C R. Effects of lignin on root activity of Malus hupehensis rehd. and microecology in rhizosphere soil under organic fertilizer. Journal of Shandong Agricultural University (Natural Science Edition), 2018, 49(4): 561-565. (in Chinese)
[43]	孙瑞敏, 翟梦华, 李虎, 李孟华, 王凤, 张桦. 木质素合成与梭梭生长发育的关系研究. 现代农业科技, 2019(5): 126-127.
	SUN R M, ZHAI M H, LI H, LI M H, WANG F, ZHANG H. Study on the relationship between lignin synthesis and Haloxylon ammodendron growth and development. XianDai NongYe KeJi, 2019(5): 126-127. (in Chinese)
[44]	KATHARE P K, DHARMASIRI S, DHARMASIRI N. SAUR53 regulates organ elongation and apical hook development in Arabidopsis. Plant Signaling & Behavior, 2018, 13(10): e1514896.
[45]	WONG J H, SPARTZ A K, PARK M Y, DU M M, GRAY W M. Mutation of a conserved motif of PP2C.D phosphatases confers SAUR immunity and constitutive activity. Plant Physiology, 2019, 181(1): 353-366. doi: 10.1104/pp.19.00496 pmid: 31311832

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

基因 Gene	引物 Primer sequences (5'-3')
PpActin	F: GCCCAAGTGCTTCGTATGCT
PpActin	R: ATCACCGGCTGCAATCCA
PpSAUR73	F: GGCACCCAACTGAGAAGTGA
PpSAUR73	R: ACGAGGTTCCAAAAGGGCAT

基因ID Gene ID	Log₂FC （CK/SAUR73-1）	Log₂FC （CK/SAUR73-14）	描述 Description
AT1G05260	1.20742752491825	1.00323218173246	过氧化物酶超家族蛋白 Peroxidase superfamily protein
AT1G26390	-2.70470526776539	-3.79852645503335	FAD结合黄连素家族蛋白 FAD-binding Berberine family protein
AT1G66270	1.56761594119799	1.4138309067234	糖基水解酶超家族蛋白 Glycosyl hydrolase superfamily protein
AT1G66280	1.32994741757618	1.28961957063889	糖基水解酶超家族蛋白 Glycosyl hydrolase superfamily protein
AT1G66800	1.24780969170763	1.32419227545878	NAD(P)结合罗斯曼折叠超家族蛋白NAD(P)-binding Rossmann-fold superfamily protein
AT2G32860	1.23824493211364	1.37258943978524	β-葡萄糖苷酶33 Beta glucosidase 33
AT3G01190	1.34813864857128	1.07544938464363	过氧化物酶超家族蛋白 Peroxidase superfamily protein
AT3G32980	1.38165803707019	1.11752278178902	过氧化物酶超家族蛋白 Peroxidase superfamily protein
AT3G49110	1.28803391956951	1.78265014560853	过氧化物酶CA peroxidase CA
AT5G15180	1.34289458873555	1.22134563236366	过氧化物酶超家族蛋白 Peroxidase superfamily protein
AT5G28510	1.77489118882188	1.59816016112596	β-葡萄糖苷酶24 Beta glucosidase 24

Functional Identification of Peach Gene PpSAUR73

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 13

References 45

Related Articles 15

Metrics

Comments

Recommended 0

[1]	WANG JiaNuo, CHEN GuiPing, LI Pan, WANG LiPing, NAN YunYou, HE Wei, FAN ZhiLong, HU FaLong, CHAI Qiang, YIN Wen, ZHAO LiaoHao. Photo-Physiological Mechanism at Grain Filling Stage of No-Tillage with Plastic Re-Mulching to Increase Maize Yield in Oasis Irrigation Areas [J]. Scientia Agricultura Sinica, 2026, 59(6): 1189-1202.
[2]	CUI ShiYou, CHEN PengJun, MIAO YuanQing, HAN JiJun, SHEN JunMing. Development and Field Evaluation of Glyphosate-Resistant Wheat Germplasm Generated Through EMS Mutagenesis [J]. Scientia Agricultura Sinica, 2026, 59(4): 723-733.
[3]	LIAO TingLu, SHI YaFei, XIAO DongHao, SHE YangMengFei, GUO FuCheng, YANG JiuJu, TANG HaiJiang, LUO ChengKe. The Effect of Exogenous Nitroprusside on Sugar Metabolism in Rice Seedlings Under Alkaline Stress [J]. Scientia Agricultura Sinica, 2026, 59(2): 265-277.
[4]	SUN Ping, ZHU WenCan, LIN XianRui, WU JiaQi, CAO YiWen, CHEN ChenFei, WANG Yi, ZHU JianXi, JIA HuiJuan, QIAN MinJie, SHEN JianSheng. Effects of Rainy and Low Light Conditions on Coloration and Flavonoid Accumulation in Peach Peel Based on Metabolomic and Transcriptomic Analyses [J]. Scientia Agricultura Sinica, 2025, 58(6): 1173-1194.
[5]	ZHANG TianYu, LI Bai, ZANG JinPing, CAO HongZhe, DONG JinGao, XING JiHong, ZHANG Kang. Genome-Wide Identification and Expression Analysis of HMG Family Genes in Botrytis cinerea [J]. Scientia Agricultura Sinica, 2025, 58(4): 704-718.
[6]	ZHANG LinLin, GONG Rui, CUI YanLing, ZHONG XiongHui, LI Ye, LI RanHong, QIAN ZongWei. Effect Analysis of SmWRKY30 in Eggplant Resistance to Ralstonia solanacearum by Virus Induced Gene Silencing (VIGS) [J]. Scientia Agricultura Sinica, 2025, 58(3): 548-563.
[7]	ZHANG XiangKun, LI JiaYing, QIAO RuMeng, HE JingLei, WANG Li, SHI XiaoXin, DU GuoQiang. Effects of GFabV Under Different Zn Levels on Photosynthetic Efficiency and Photosynthesis-Related Gene Expression of ‘Shine Muscat’ Grapevine [J]. Scientia Agricultura Sinica, 2025, 58(24): 5190-5200.
[8]	DING Ning, QI EnFang, JIA XiaoXia, HUANG Wei, MA LiRong, LI JianWu, YAN RuNan. Screening and Identification of miRNAs in Potato Seedlings in Response to High Temperature Stress [J]. Scientia Agricultura Sinica, 2025, 58(22): 4589-4602.
[9]	GUO TianFa, WU JinLong, QIU QianQian, MA XinChao, WANG LiRong, WU CuiYun. Relationship Between the Formation of Non-Red Color in the Fruit Skin of Xinjiang Local Peach Varieties and the Variation of PpMYB10.1 Promoter [J]. Scientia Agricultura Sinica, 2025, 58(2): 326-338.
[10]	ZHANG Jie, HU ChenXi, QI JianBo, ZHANG YongTai, CHEN YiBo, ZHANG YongJi. Effects of Exogenous Zeatin on Photosynthetic Parameter, Antioxidant System and Expression of Genes Related to Zeatin Synthesis in Pepper Under Low-Temperature Combined with Low-Light Stress [J]. Scientia Agricultura Sinica, 2025, 58(19): 3959-3969.
[11]	GUO Lei, ZHANG BinBin, SHEN ZhiJun, YAN Juan, XU JianLan, CAI ZhiXiang, YU MingLiang, WANG FaLin, SONG HongFeng. The Release Characteristics of Medium and Trace Elements and Their Effects on Soil Available Nutrients after the Continuous Return of Green Manure in Peach Orchards [J]. Scientia Agricultura Sinica, 2025, 58(12): 2411-2426.
[12]	GUO Lei, HUANG ChenYan, SONG HongFeng, SHEN ZhiJun, ZHANG BinBin, MA RuiJuan, SUN Meng, HE Xin, YU MingLiang. Screening, Compounding and Safety Evaluation of Herbicides Suitable for Peach Nursery [J]. Scientia Agricultura Sinica, 2024, 57(9): 1734-1747.
[13]	CHEN BingXian, ZHANG Qi, DAI ZhangYan, ZHOU Xu, LIU Jun. Physiological and Molecular Effects of Salicylic Acid on Rice Seed Germination at Low Temperature [J]. Scientia Agricultura Sinica, 2024, 57(7): 1220-1236.
[14]	WANG ChengZe, ZHANG Yan, FU Wei, JIA JingZhe, DONG JinGao, SHEN Shen, HAO ZhiMin. Bioinformatics and Expression Pattern Analysis of Maize ACO Gene Family [J]. Scientia Agricultura Sinica, 2024, 57(7): 1308-1318.
[15]	SU XiaoYu, TAN ZhengWei, LI ChunMing, LI Lei, LU DanDan, YU YongLiang, DONG Wei, AN SuFang, YANG Qing, SUN Yao, XU LanJie, YANG HongQi, LIANG HuiZhen. Analysis of Genome-Wide Methylation Differences and Associated Gene Expression of Sesame Varieties Under High Temperature Stress [J]. Scientia Agricultura Sinica, 2024, 57(24): 4825-4838.