乙烯促进牡丹‘洛阳红’切花花瓣脱落与内源生长素的关联性分析

doi:10.3864/j.issn.0578-1752.2021.23.014

Abstract

Abstract:

【Objective】The aim of this study was to explore the physiological mechanisms of petal wilting and abscission during the senescence of cut tree peony flowers, so as to provide the theoretical basis for better development of storage and preservation technology of cut tree peony flowers.【Method】The cut flowers were pretreated with deionized water (CK), 20 µL·L^-1 ethylene antagonist Correxian (AVB), 20 µL·L^-1 ethylene releasing agent ethephon (CEPA), and 20 µL·L^-1 Correxian for 1 h, respectively, followed by 20 µL·L^-1 ethephon 1 h (AVB+CEPA). The flowers were then placed in individual vases filled with distilled water. The morphology of the petals absciss zone cells were observed by using a microscope. The petal anti-falling ability was determined by using the tensile test during the vase cutting process. The physiological effects of different pretreatments were observed respectively using the physiological and biochemical methods to determine the ethylene and auxin metabolism, the absciss zone hydrolysis-related enzyme activities, and the relative expressions of PsETR1, PsCTR1, PsEIN3, PsERF1, PsYUCCA10, PsPIN1, PsPME1, PsPG1 and PsBG1 genes. 【Result】 The results showed that the basal petal of the cut tree peony Luoyanghong had a clear separation layer structure. AVB significantly delayed the occurrence of ethylene climacteric in cut flowers, and decreased the ethylene release peak by 34.9% (P<0.05), while the petal anti-falling ability was improved. The activities of polygalaturonase (PG), β-glucosidase (BG) and the expressions of PsPG1 and PsBG1 genes at the base of the petals absciss zone were reduced, which delayed the senescence process of cut tree peony flowers, thus significantly extended its vase life. Nevertheless, the exogenous CEPA significantly accelerated the release rate of endogenous ethylene in the petals, increased the activities of IAA oxidase (IAAO), PG and BG, accelerated the senescence of the absciss zone cells in the petals of cut flowers, and reduced the petal anti-falling ability, thus promoted its wilting and falling. Meanwhile, the vase life and the optimal viewing period of AVB+CEPA composite pretreatment were not much different from control, so CEPA could partially offset the physiological effects of AVB. 【Conclusion】 For the ethylene sensitive cut tree peony Luoyanghong, endogenous auxin was involved in the process of ethylene promoting the abscission of petal absciss zone cells. The control of ethylene was the basic way to improve the quality of Luoyanghong cut flowers.

Key words: tree peony, cut flowers, absciss zone, ethylene, auxin, abscission

YE Di,SHI Jiang,GAO ShuangCheng,WANG ZhanYing,SHI GuoAn. Correlation Analysis of Auxin Involved in the Process of Petal Abscission of Tree Peony Luoyanghong Cut Flowers by Ethylene Promoting[J].Scientia Agricultura Sinica, 2021, 54(23): 5097-5109.

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基因 Gene	正向引物序列（5′-3′） Foward primer sequence	反向引物序列（5′-3′） Reverse primer sequence	预期大小 Expected size (bp)
PsD-GAPDH	TGTTCACTCCATCACTGCTAC	ACATCCACAGTAGGAACACGA	189
PsETR1	CGTGGGTCGTCAGAGTGTGT	CCTTGCACCTGCCGTTCTTG	153
PsCTR1	CTCAACCCAGCCGTACAGACAT	CGGGCAGTAAACAACAGAAAAT	182
PsEIN3	TTTGTGAGCTTTGGGGGTGT	GCCAACTCTGCAACACGAAA	190
PsERF1	AGTCGGAAAGGAGCTAGGGT	TTCAGACACCGACGAACACC	147
PsYUCCA10	CAACATACGTGCCAAGAG	GGTAGCCACCACCAGAAA	206
PsPIN1	GGCCGTTGTCCCTCTCTATG	AAAGGGGTACGGCGAAGATG	125
PsPME1	ATGCTTGGACGGTCTGAT	GCTGGAGGTATGGATTTC	193
PsPG1	GATGCGTCTTCTCTTCCT	GGTTTCGTCACATTCTAC	190
PsBG1	ATTTCTACTCCCCGCCAT	GGGGCTTTGTTGTTCACT	195

预处理 Pretreatment	瓶插寿命 Vase life (d)	最佳观赏期 Best viewing period (d)	最大花径 Maximum diameter (cm)
CK	5.9±0.3a	4.5±0.5a	11.15±2.2b
AVB	6.0±0.0a	4.5±0. 5a	12.19±1.6ab
CEPA	4.8±0.4b	4.1±0.6b	12.84±1.6a
AVB+CEPA	5.8±0.4ab	4.3±0.6ab	12.84±1.3a

Correlation Analysis of Auxin Involved in the Process of Petal Abscission of Tree Peony Luoyanghong Cut Flowers by Ethylene Promoting

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