Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (5): 919-932.doi: 10.3864/j.issn.0578-1752.2016.05.012

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• HORTICULTURE • Previous Articles     Next Articles

Differential Protein Analysis of Tomato Leaves in Tomato-Potato Onion Intercropping System

LIU Shu-qin1,2, ZHOU Xin-gang1, WU Feng-zhi1, LIU Shou-wei1   

  1. 1Horticulture College, Northeast Agricultural University, Harbin 150030;
    2    School of Life Science, Baicheng Normal University, Baicheng 137000, Jilin
  • Received:2015-04-27 Online:2016-03-01 Published:2016-03-01

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Abstract: 【Objective】The objective of this study was to better understand the soil sickness mechanism of the tomato intercropped with four different allelopathic potential potato onions which has high yield and less plant diseases, obtain the differential expressed proteins of tomato leaves in tomato-potato onion intercropping system, and improve the foundation and extend of tomato cultivation mode.【Method】 A comparative proteome analysis was conducted to study the impact of tomatoes under normal monoculture and intercropping with four varieties of potato onion with different allelopathic potential (high allelopathic potential cultivars: ‘Suihua’, ‘Wuchanghongqishe’, and low allelopathic potential cultivars: ‘Ninganhongcheng’, ‘Qitaihe’). Total proteins were extracted from these tomato leaves, and subjected to two-dimensional electrophoresis, followed by MALDI-TOF-MS mass spectrometry. Types and functions of proteins differently expressed in the leaves were analyzed.【Result】The results showed that 39 proteins differently expressed in the leaves of intercropped tomato. These proteins were divided into main 7 categories, including proteins related to photosynthesis, metabolism, energy metabolism, plant resistance, protein synthesis, nucleic acid synthesis and unknown protein. The proteins showing a higher abundance were primarily involved in 11 photosynthesis proteins, 10 metabolic proteins and 8 plant resistance proteins discovered. The photosynthesis of intercropped tomato enhanced and resistance also increased. The expression levels of heat shock-related protein, thioredoxin peroxidase, polyphenoloxidase, sedoheptulose-1,7- bisphosphatase, S-adenosylmethionine synthase, ATP-binding protein increased in the leaves of intercropped tomato. Interestingly, the expression levels of these proteins from the tomato intercropped with potato onions with high allelopathic potentials were significantly higher than that intercropped with potato onions with low allelopathic potentials.【Conclusion】Our results showed that potato onion with different allelopathic potentials intercropped have different influences on tomato at the protein expression. The tomato intercropped with potato onions with high allelopathic potentials were stronger than tomato intercropped with potato onions with low allelopathic potentials, and is an effective way for promoting tomatoes healthy growth and better plant resistance．

Key words: tomato, potato onion, intercropping, differential protein

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