Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (4): 668-677.doi: 10.3864/j.issn.0578-1752.2013.04.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis on the Genetic Diversity in Chinese Barley Landrace and Genomic Wide Association of α-amylase Activity Genes

 JIANG  Xiao-Dong, GUO  Gang-Gang, ZHANG  Jing   

  1. 1.中国农业科学院作物科学研究所，北京 100081
    2.山西农业大学农学院，山西太谷 030801
  • Received:2012-12-03 Online:2013-02-15 Published:2013-01-23

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Abstract: 【Objective】The objective of this study is to understand genetic diversity in Chinese barley landraces and screen valuable molecular marker for α-amylase activity.【Method】 A total 257 Chinese barley landraces were genotyped with 41 SSR markers distributing on seven chromosomes of barley. Nei’s genetic distance and neighbour-joining were adoped in cluster analysis.【Result】A total of 709 alleles were identified at 41 SSR loci in 257 Chinese barley landraces. The number of alleles per locus ranged from 5 to 44, with an average of 17. The polymorphism information content value for SSRs ranged from 0.23 (Bmag 0385) to 0.94(Bmac0032) with a mean of 0.6385. Cluster analysis detected that nine clusters were identified based on Nei’s genetic distance in 257 accessions. Association analysis showed that five SSR markers significantly associated with α–amylase activity. 【Conclusion】 Chinese barley landraces have rich genetic diversity and could be clustered into nine different groups based on morphological characteristics, geographic origin and genetic relationships. Among the five SSR markers associated with α–amylase activity, Bmag0385 on chromosome-7H carrying allele A215 possessed the highest α-amylase activity, and Bmac0273 on 7H carrying with allele A141 had relatively high positive effect on α-amylase activity. All of them could be used in marker-assisted selection for malting barley breeding.

Key words: Chinese barley , genetic diversity , allele effect , α-amylase activity , association mapping

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