Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (18): 3784-3792.doi: 10.3864/j.issn.0578-1752.2013.18.006

• PLANT PROTECTION • Previous Articles     Next Articles

Genetic Diversity of Two Hypervariable Genes from Prophage Regions of ‘Candidatus Liberibacter asiaticus’ in China

 TAN  Jin-12, WANG  Xue-Feng-2, SU  Hua-Nan-12, LI  Zhong-An-2, ZHOU  Chang-Yong-2   

  1. 1.College of Plant Protection, Southwest University, Chongqing 400716
    2.Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712
  • Received:2013-02-26 Online:2013-09-15 Published:2013-06-04

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Abstract: 【Objective】 Hypervariable genomic loci of prophage region were used to study population differentiation and genetic diversity of ‘Candidatus Liberibacter asiaticus’, the putative casual agent of citrus Huanglongbing (HLB). 【Method】 Two primer sets (Lap-TJ-F/Lap-TJ-R1 and Lap-TJ-F/Lap-TJ-R2), targeted hypervariable genomic regions of two prophage types (SC1 and SC2), respectively, were used for PCR detection and sequence analysis of 224 ‘Ca. L. asiaticus’ strains from different citrus producing areas in China. 【Result】 PCR amplicon polymorphisms and four amplion types (SC1-1, SC1-2, SC2-1 and SC2-2) were observed, SC1-1 and SC2-1 were the predominant types in Southwestern region and Guangdong-Guangxi region, respectively, and no dominant types were found in Fujian-Jiangxi-Zhejiang region. The sequence differences in SC1-1/SC1-2 were mainly caused by different combinations of two kinds of tandem repeats with 132 bp satellite sequence and 32 bp mini-satellite sequence, respectively. The diversity in SC2-1/SC2-2 likely derived from prophage gene rearrangement. 【Conclusion】Significant diversity in the prophage regions was investigated from ‘Ca. L. asiaticus’ strains collected from different geographical origins. Further studies on these genomic regions would be helpful for deciphering the genetic diversity of ‘Ca. L. asiaticus’ populations in China.

Key words: citrus Huanglongbing (HLB) , prophage , tandem repeat , genetic diversity

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