Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (12): 2543-2549.doi: 10.3864/j.issn.0578-1752.2013.12.015

• ANIMAL SCIENCE·VETERINARY SCIENCERE • Previous Articles     Next Articles

Effects of HMGCS1 Gene on Skeletal Muscle Growth and Development and Regulated by miR-18a/b of Pig

 SUN  Wei, TANG  Zhong-Lin, TAN  Lin, LEI  Chu-Chao, LI  Kui   

  1. 1.College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi
    2.Institute of Animal Science, Chinese Academy of Agricultural Sciences/State Key Laboratory of Farm Animal Genetic Resources and Utilization, Beijing 100193
  • Received:2012-12-06 Online:2013-06-15 Published:2013-04-02

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Abstract: 【Objective】The purpose of the present research was to explore whether HMGCS1 impacts the skeletal muscle growth and development of pig and validate its target gene.【Method】In this study，the expression of HMGCS1 in different tissues and the expression change during Longissimus dorsi muscle development (28 stages) was investigated by real-time qPCR.【Result】The result showed that the HMGCS1 gene was strongly expressed in lung of adult pig, and the expression level of HMGCS1 gene in embryonic period 33 d and 65 d were higher than those in other periods in Tongcheng pigs, and lower expression at postnatal 20 d, 30 d, 40 d and 60 d. The luciferase activity was lower in miR-18a/b minics than in the control.【Conclusion】The results of study indicated that HMGCS1 regulates skeletal muscle growth and development by miR-18a/b, and is a potential candidate target gene of meat production traits.

Key words: pig , HMGCS1 gene , miR-18a/b , skeletal muscle , growth and development

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