FoxO1对牛骨骼肌细胞增殖、凋亡和分化的调控

doi:10.3864/j.issn.0578-1752.2024.06.013

Abstract

Abstract:

【Objective】 Skeletal muscle is an important component of animal body, and its growth and development directly affect the meat yield of livestock and poultry. As an important transcription regulator, fork head box protein O1 (FoxO1) is closely related to the growth and development of skeletal muscle. Exploring the effects of overexpression of FoxO1 gene on the proliferation, apoptosis and differentiation of bovine skeletal muscle cells could provide the theoretical basis for genetic improvement of beef. 【Method】 The tissue expression profile of FoxO1 was constructed by real-time fluorescence quantitative PCR (qPCR). To test the cell differentiation performance of bovine skeletal muscle cells which were isolated by enzyme digestion, the myotubes formation was observed and the expressions of differentiation marker genes were detected by qPCR. The subcellular localization of bovine skeletal muscle cells was carried out by immunofluorescence technique. In order to improve the expression of FoxO1 in bovine skeletal muscle cells, bovine FoxO1 overexpression adenovirus were designed and packaged. The effect of FoxO1 overexpression on the relative proliferation rate of cells was detected by EdU staining. Flow cytometry was used to detect the effect of FoxO1 overexpression on cell cycle distribution. The effects of FoxO1 overexpression on the expression levels of genes related to proliferation, apoptosis and differentiation of bovine skeletal muscle cells were investigated by qPCR.【Result】 The results of tissue expression spectrum showed that FoxO1 was expressed in many tissues, with the highest expression in the back fat of adult cattle and the lowest expression in the longissimus dorsi muscle tissue, while the expression of FoxO1 in the longissimus dorsi muscle tissue of calves was significantly higher than that of adult cattle (P<0.01). The results of subcellular localization showed that FoxO1 was expressed in the nucleus and cytoplasm of bovine skeletal muscle cells, and the fluorescence intensity in the nucleus was higher than that in the cytoplasm. The FoxO1 overexpression vector was successfully constructed, and the packaging and propagation of FoxO1 recombinant adenovirus were completed. After infecting bovine skeletal muscle cells, the FoxO1 expression level was significantly improved (P<0.01). EdU detection showed that overexpression of FoxO1 significantly reduced the cell proliferation rate (P<0.01), and flow cytometry showed that overexpression of FoxO1 significantly increased the number of cells in G1 phase and decreased the number of cells in S phase and G2 phase, inhibited the transformation of cells in G1/S phase and reduced the formation of cells in G2 phase. Further detection by qPCR found that the proliferation-related genes of PCNA, CDK1, CDK2, CCNA2, CCNB1, CCND1 and CCNE2 were significantly down-regulated (P<0.01), the apoptosis-related genes BAD and BAX were significantly up-regulated, and the apoptosis-inhibiting gene BCL2 was significantly down-regulated (P<0.05). After FoxO1 was overexpressed, the myotube formation of bovine skeletal muscle cells decreased, and the expression levels of MYOD, MYOG, MYF5, MYF6 and MYHC related to skeletal muscle differentiation were significantly decreased by qPCR detection (P<0.05). 【Conclusion】 FoxO1 was expressed in different tissues of cattle, and it was a ubiquitous transcription regulator. There were differences in expression at different stages of the growth and development of longissimus dorsi muscle, which played a role in stage regulation. FoxO1 played a transcriptional regulatory role in both the nucleus and cytoplasm, especially in the nucleus. Overexpression of FoxO1 might inhibit the proliferation and differentiation of bovine skeletal muscle cells by inhibiting the expression of genes related to cell proliferation and muscle cell differentiation, and might promote the apoptosis of bovine skeletal muscle cells by up-regulating the expression of apoptosis-promoting genes and down-regulating the expression of apoptosis- inhibiting genes.

Key words: FoxO1, bovine skeletal muscle cells, proliferation, differentiation

JIANG Chao, ZHANG JiuPan, SONG YaPing, SONG XiaoYu, WU Hao, WEI DaWei. Study on the Role of FoxO1 in Regulating the Proliferation, Apoptosis and Differentiation of Bovine Skeletal Muscle Cells[J].Scientia Agricultura Sinica, 2024, 57(6): 1191-1203.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2024.06.013

https://www.chinaagrisci.com/EN/Y2024/V57/I6/1191

Figures/Tables 12

Table 1

Table 2

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

Fig. 9

Fig. 10

References 31

[1]	张天留, 葛菲, 朱波, 高会江, 李俊雅, 高雪. 肉牛种业科技创新发展现状与趋势分析. 中国畜禽种业, 2022, 18(10): 5-16.
	ZHANG T L, GE F, ZHU B, GAO H J, LI J Y, GAO X. Present situation and trend analysis of scientific and technological innovation in beef cattle seed industry. The Chinese Livestock and Poultry Breeding, 2022, 18(10): 5-16. (in Chinese)
[2]	付玉, 张博, 凌遥, 张浩. 骨骼肌生长发育过程及调控研究现状. 中国畜牧兽医, 2021, 48(10): 3565-3574. doi: 10.16431/j.cnki.1671-7236.2021.10.007
	FU Y, ZHANG B, LING Y, ZHANG H. Reviews on process and regulation of skeletal muscle growth and development. China Animal Husbandry & Veterinary Medicine, 2021, 48(10): 3565-3574. (in Chinese)
[3]	AHMAD S S, CHUN H J, AHMAD K, SHAIKH S, LIM J H, ALI S, HAN S S, HUR S J, SOHN J H, LEE E J, CHOI I. The roles of growth factors and hormones in the regulation of muscle satellite cells for cultured meat production. Journal of Animal Science and Technology, 2023, 65(1): 16-31. doi: 10.5187/jast.2022.e114 pmid: 37093925
[4]	MIERZEJEWSKI B, GRABOWSKA I, JACKOWSKI D, IRHASHAVA A, MICHALSKA Z, STREMIŃSKA W, JAŃCZYK-ILACH K, CIEMERYCH M A, BRZOSKA E. Mouse CD146+ muscle interstitial progenitor cells differ from satellite cells and present myogenic potential. Stem Cell Research & Therapy, 2020, 11(1): 341.
[5]	MOHAMMADABADI M, BORDBAR F, JENSEN J, DU M, GUO W. Key genes regulating skeletal muscle development and growth in farm animals. Animals, 2021, 11(3): 835. doi: 10.3390/ani11030835
[6]	ESTEVES DE LIMA J, RELAIX F. Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation. Cell Regeneration, 2021, 10(1): 31. doi: 10.1186/s13619-021-00093-5
[7]	ZAMMIT P S. Function of the myogenic regulatory factors Myf5, MyoD, Myogenin and MRF₄ in skeletal muscle, satellite cells and regenerative myogenesis. Seminars in Cell & Developmental Biology, 2017, 72: 19-32.
[8]	SHIRAKAWA T, TOYONO T, INOUE A, MATSUBARA T, KAWAMOTO T, KOKABU S. Factors regulating or regulated by myogenic regulatory factors in skeletal muscle stem cells. Cells, 2022, 11(9): 1493. doi: 10.3390/cells11091493
[9]	侯任达, 张润, 侯欣华, 王立贤, 张龙超. 畜禽肌纤维发育规律及相关基因研究进展. 畜牧兽医学报, 2022, 53(10): 3279-3286.
	HOU R D, ZHANG R, HOU X H, WANG L X, ZHANG L C. Research progress on the pattern of muscle fiber development and related genes in livestock and poultry. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3279-3286. (in Chinese)
[10]	束婧婷, 姬改革, 单艳菊, 章明, 肖芹, 屠云洁, 盛中伟, 张笛, 邹剑敏. 基于表达谱芯片挖掘鸡骨骼肌不同类型肌纤维的差异表达基因. 中国农业科学, 2017, 50(14): 2826-2836. doi:10.3864/j.issn.0578-1752.2017.14.018.
	SHU J T, JI G G, SHAN Y J, ZHANG M, XIAO Q, TU Y J, SHENG Z W, ZHANG D, ZOU J M. Analysis of differential expression genes between different myofiber types in chicken skeletal muscle based on gene expression microarray. Scientia Agricultura Sinica, 2017, 50(14): 2826-2836. doi:10.3864/j.issn.0578-1752.2017.14.018. (in Chinese)
[11]	李燕, 陈明明, 张俊星, 张林林, 李新, 郭宏, 丁向彬, 刘新峰. 牛LncRNA-133a对骨骼肌卫星细胞增殖分化的影响. 中国农业科学, 2019, 52(1): 143-153. doi: 10.3864/j.issn.0578-1752.2019.01.013.
	LI Y, CHEN M M, ZHANG J X, ZHANG L L, LI X, GUO H, DING X B, LIU X F. Effects of bovine LncRNA-133a on the proliferation and differentiation of skeletal muscle satellite cells. Scientia Agricultura Sinica, 2019, 52(1): 143-153. doi: 10.3864/j.issn.0578-1752.2019.01.013. (in Chinese)
[12]	CALNAN D R, BRUNET A. The FoxO code. Oncogene, 2008, 27(16): 2276-2288. doi: 10.1038/onc.2008.21 pmid: 18391970
[13]	GUO S C, MANGAL R, DANDU C T, GENG X K, DING Y C. Role of forkhead box protein O1 (FoxO1) in stroke: a literature review. Aging and Disease, 2022, 13(2): 521-533. doi: 10.14336/AD.2021.0826
[14]	YAMASHITA A, HATAZAWA Y, HIROSE Y, ONO Y, KAMEI Y. FOXO 1 delays skeletal muscle regeneration and suppresses myoblast proliferation. Bioscience, Biotechnology, and Biochemistry, 2016, 80(8): 1531-1535. doi: 10.1080/09168451.2016.1164585
[15]	ZHANG H, CHI M Y, CHEN L L, SUN X P, WAN L L, YANG Q J, GUO C. Daidzein alleviates cisplatin-induced muscle atrophy by regulating Glut4/AMPK/FoxO pathway. Phytotherapy Research, 2021, 35(8): 4363-4376. doi: 10.1002/ptr.7132 pmid: 33876509
[16]	WANG B, ZHANG A Q, WANG H D, KLEIN J D, TAN L, WANG Z M, DU J, NAQVI N, LIU B C, WANG X H. miR-26a limits muscle wasting and cardiac fibrosis through exosome-mediated microRNA transfer in chronic kidney disease. Theranostics, 2019, 9(7): 1864-1877. doi: 10.7150/thno.29579 pmid: 31037144
[17]	RU W X, LIU K P, YANG J M, LIU J Y, QI X L, HUANG B Z, CHEN H. miR-183/96/ 182 cluster regulates the development of bovine myoblasts through targeting FoxO1. Animals, 2022, 12(20): 2799. doi: 10.3390/ani12202799
[18]	ZHANG X J, JIANG L S, LIU H M. Forkhead box protein O1: functional diversity and post-translational modification, a new therapeutic target? Drug Design, Development and Therapy, 2021, 15: 1851-1860. doi: 10.2147/DDDT.S305016 pmid: 33976536
[19]	LIU Y, WANG Y, SHAN T, GUO J, XU C, LIU J. The tissue-specific and developmental expressionpatterns of the forkhead transcription factor FoxO1gene in pigs. Journal of Animal and Feed Sciences, 2008, 17(2): 182-190. doi: 10.22358/jafs/66598/2008
[20]	PANG W J, YU T Y, BAI L, YANG Y J, YANG G S. Tissue expression of porcine FoxO1 and its negative regulation during primary preadipocyte differentiation. Molecular Biology Reports, 2009, 36(1): 165-176. doi: 10.1007/s11033-007-9163-6
[21]	YOSHIMOCHI K, DAITOKU H, FUKAMIZU A. PCAF represses transactivation function of FOXO1 in an acetyltransferase-independent manner. Journal of Receptor and Signal Transduction Research, 2010, 30(1): 43-49. doi: 10.3109/10799890903517947 pmid: 20041807
[22]	WU Y J, FANG Y H, CHI H C, CHANG L C, CHUNG S Y, HUANG W C, WANG X W, LEE K W, CHEN S L. Insulin and LiCl synergistically rescue myogenic differentiation of FoxO1 over- expressed myoblasts. PLoS ONE, 2014, 9(2): e88450. doi: 10.1371/journal.pone.0088450
[23]	SCHACHTER T N, SHEN T S, LIU Y W, SCHNEIDER M F. Kinetics of nuclear-cytoplasmic translocation of Foxo1 and Foxo3A in adult skeletal muscle fibers. American Journal of Physiology Cell Physiology, 2012, 303(9): C977-C990. doi: 10.1152/ajpcell.00027.2012
[24]	CHEN J Y, LU Y, TIAN M Y, HUANG Q R. Molecular mechanisms of FOXO1 in adipocyte differentiation. Journal of Molecular Endocrinology, 2019, 62(3): R239-R253. doi: 10.1530/JME-18-0178
[25]	MONSALVE M, OLMOS Y. The complex biology of FOXO. Current Drug Targets, 2011, 12(9): 1322-1350. doi: 10.2174/138945011796150307 pmid: 21443460
[26]	LIU F, QU R F, YANG L M, SHI G, HAO S H, HU C M. Circular RNA controls tumor occurrence and development via cell cycle regulation. OncoTargets and Therapy, 2022, 15: 993-1009. doi: 10.2147/OTT.S371629 pmid: 36134387
[27]	HUANG H J, TINDALL D J. CDK2 and FOXO1: a fork in the road for cell fate decisions. Cell Cycle, 2007, 6(8): 902-906. pmid: 17457058
[28]	GEBREMEDHN S, SALILEW-WONDIM D, HOELKER M, RINGS F, NEUHOFF C, THOLEN E, SCHELLANDER K, TESFAYE D. microRNA-183-96-182 cluster regulates bovine granulosa cell proliferation and cell cycle transition by coordinately targeting FOXO1. Biology of Reproduction, 2016, 94(6): 127, 1-11.
[29]	CIFARELLI V, LEE S, KIM D H, ZHANG T, KAMAGATE A, SLUSHER S, BERTERA S, LUPPI P, TRUCCO M, DONG H H. FOXO 1 mediates the autocrine effect of endothelin-1 on endothelial cell survival. Molecular Endocrinology, 2012, 26(7): 1213-1224. doi: 10.1210/me.2011-1276
[30]	FERRI P, BARBIERI E, BURATTINI S, GUESCINI M, D'EMILIO A, BIAGIOTTI L, DEL GRANDE P, DE LUCA A, STOCCHI V, FALCIERI E. Expression and subcellular localization of myogenic regulatory factors during the differentiation of skeletal muscle C2C12 myoblasts. Journal of Cellular Biochemistry, 2009, 108(6): 1302-1317. doi: 10.1002/jcb.22360 pmid: 19830700
[31]	来裕婷, 朱菲菲, 王轶敏, 郭宏, 张林林, 李新, 郭益文, 丁向彬. PSMB5蛋白对牛骨骼肌卫星细胞增殖与成肌分化的影响. 中国农业科学, 2020, 53(20): 4287-4296. doi: 10.3864/j.issn.0578-1752.2020.20.016.
	LAI Y T, ZHU F F, WANG Y M, GUO H, ZHANG L L, LI X, GUO Y W, DING X B. Effects of PSMB5 on the proliferation and myogenic differentiation of skeletal muscle satellite cells. Scientia Agricultura Sinica, 2020, 53(20): 4287-4296. doi: 10.3864/j.issn.0578-1752.2020.20.016. (in Chinese)

Related Articles 15

[1]	YUAN Miao, ZHOU Juan, DANG ShiZhuo, TANG XueShen, ZHANG YaHong. Functional Analysis of VvARF18 Gene in Red Globe Grape [J]. Scientia Agricultura Sinica, 2024, 57(7): 1363-1376.
[2]	LI KaiLi, WEI YunXiao, CHONG ZhiLi, MENG ZhiGang, WANG Yuan, LIANG ChengZhen, CHEN QuanJia, ZHANG Rui. Red and Blue Light Promotes Cotton Callus Induction and Proliferation [J]. Scientia Agricultura Sinica, 2024, 57(4): 638-649.
[3]	TIAN QingLan, ZHOU JunNiu, WU YanYan, LIU JieYun, HUANG WeiHua, ZHANG YingJun, XIE WenLian, WEI GuangTan, MOU HaiFei. Observation of Flower Bud Differentiation Process and Fitting of Flower Growth Model of Passion Fruit [J]. Scientia Agricultura Sinica, 2024, 57(4): 765-778.
[4]	ZHANG Peng, WANG MingXiu, JING KeMin, LI YuQian, TIAN Yuan, ZHONG JinCheng, CAI Xin. Cloning of PLZF Gene and Its Effects on the Proliferation of Undifferentiated Spermatogonia in Cattleyak [J]. Scientia Agricultura Sinica, 2024, 57(2): 390-402.
[5]	WANG JinPeng, LUORENG ZhuoMa, LI YanXia, FENG Fen, WANG ZhengXing, PAN ChuanYing, LAN XianYong, WANG XingPing. The Function of lncRNA RRAS2-AS1 in LPS Induced Bovine Mammary Epithelial Cells Inflammation [J]. Scientia Agricultura Sinica, 2024, 57(14): 2874-2888.
[6]	ZHU BingLin, YU JiaLi, CHEN JiaYue, TIAN Yuan, WAN Yuan, LIU ChenYang, WANG XiaoYu, WANG MiaoLi, CHENG Gong. Cloning, Expression Characterization, and Functional Analysis of the Snail1 in Qinchuan Cattle and Its Impact on Proliferation of Bovine Adipocytes [J]. Scientia Agricultura Sinica, 2024, 57(13): 2674-2686.
[7]	SHU XiaoWei, WANG ShuShen, FU Tong, WANG ZiHan, DING ZhouYu, YANG Ying, ZHAO ShiRu, ZHOU Juan, HUANG JianYe, YAO YouLi, WANG YuLong, DONG GuiChun. Response Difference and Its Cause Reasons for Simplified Panicle Fertilization in Different Rice Varieties After Wheat Straw Return [J]. Scientia Agricultura Sinica, 2024, 57(10): 1961-1978.
[8]	GUO YunPeng, TAN HaoYun, GUO Hong, FU MengYun, LI Xin, HU DeBao, ZHANG LinLin, DING XiangBin, GUO YiWen. LNC721 Targeted Regulation MMP9 Affects Bovineskeletal Muscle Satellite Cell Proliferation and Differentiation [J]. Scientia Agricultura Sinica, 2023, 56(24): 4944-4955.
[9]	WANG Peng, LIU ZiYi, LIU YuFang, CHU MingXing. miR-535 Targets the GAB2 Gene to Promote Goat Granulosa Cell Proliferation Through Activation of the PI3K/AKT Signaling Pathway [J]. Scientia Agricultura Sinica, 2023, 56(23): 4757-4771.
[10]	LIU PeiPei, DING ShiJie, SONG WenJuan, TANG ChangBo, LI HuiXia, TANG Hong. NAC Affects Proliferation and Differentiation of Adipose-Derived Mesenchymal Stem Cells by Regulating Reactive Oxygen Species [J]. Scientia Agricultura Sinica, 2023, 56(21): 4330-4343.
[11]	WEI Yao, ZHANG RuiMen, AN Qiang, WANG LeYi, ZHANG YongWang, ZOU ChaoXia, ZHANG ErKang, MO BiYun, SHI DeShun, YANG SuFang, DENG YanFei, WEI YingMing. CircCEP85L Regulates the Proliferation and Myogenic Differentiation of Bovine MuSCs [J]. Scientia Agricultura Sinica, 2023, 56(18): 3670-3681.
[12]	YANG XinRan,MA XinHao,DU JiaWei,ZAN LinSen. Expression Pattern of m⁶A Methylase-Related Genes in Bovine Skeletal Muscle Myogenesis [J]. Scientia Agricultura Sinica, 2023, 56(1): 165-178.
[13]	WANG JiaMin,SHI JiaChen,MA FangFang,CAI Yong,QIAO ZiLin. Effects of Soy Isoflavones on the Proliferation and Apoptosis of Yak Ovarian Granulosa Cells [J]. Scientia Agricultura Sinica, 2022, 55(8): 1667-1675.
[14]	SHU JingTing,SHAN YanJu,JI GaiGe,ZHANG Ming,TU YunJie,LIU YiFan,JU XiaoJun,SHENG ZhongWei,TANG YanFei,LI Hua,ZOU JianMin. Relationship Between Expression Levels of Guangxi Partridge Chicken m6A Methyltransferase Genes, Myofiber Types and Myogenic Differentiation [J]. Scientia Agricultura Sinica, 2022, 55(3): 589-601.
[15]	CHEN Yu,ZHU HaoZhe,CHEN YiChun,LIU Zheng,DING Xi,GUO Yun,DING ShiJie,ZHOU GuangHong. Differentiation of Porcine Muscle Stem Cells in Three-Dimensional Hydrogels [J]. Scientia Agricultura Sinica, 2022, 55(22): 4500-4512.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

基因名称 Gene name	引物序列 Primer sequence (5′→3′)	产物长度 Products length (bp)	基因登录号 Genbank ID
GAPDH	F：CCAACGTGTCTGTTGTGGAT	80	NM_001034034
GAPDH	R：CTGCTTCACCACCTTCTTGA	80	NM_001034034
FoxO1	F：GCAGATTTACGAGTGGATGG	86	XM_025000053.1
FoxO1	R：TTGAATTCTTCCAGCCCG	86	XM_025000053.1
PCNA	F：GAACCTCACCAGCATGTCCA	97	NM_001034494
PCNA	R：TACTAGTGCCAACGTGTCCG	97	NM_001034494
CDK1	F：GAAGGGGTTCCTAGTACTGC	176	NM_174016
CDK1	R：ATGAACTGACCAGGAGGG	176	NM_174016
CDK2	F：GCCAGGAGTTACTTCTATGC	102	NM_001014934
CDK2	R：CTCCGTCCATCTTCATCC	102	NM_001014934
CCNA2	F：ACACAGTCACAGGACAAAGC	107	NM_001075123
CCNA2	R：TCTGAGGTAGGTCTGGTGAA	107	NM_001075123
CCNB1	F：TGGAGAGGTTGATGTTGAGC	95	NM_001045872
CCNB1	R：TCTGAGAAGGAGGAAAGTGC	95	NM_001045872
CCND1	F：TCCTCTCCTATCACCGCCTGAC	175	NM_001046273
CCND1	R：ACCTCCTCCTCCTCCTCTTCCT	175	NM_001046273
CCNE2	F：GCTTATGTCACTGATGGTGCTTG	112	NM_001015665
CCNE2	R：TTAGCCAGGAGATGACCGTTAC	112	NM_001015665
MYOD	F：GGCCGCTGTTTACTGTGGG	162	NM_001040478
MYOD	R：CAGCCGCTGGTTTGGGTT	162	NM_001040478
MYOG	F：CTATGACGGGGAGAACTACCTG	249	NM_001111325
MYOG	R：CATTCACCTTCTTGAGTCTGCG	249	NM_001111325
MYHC	F：GCCCACTTCTCCCTCATTCACT	201	XM_010815980
MYHC	R：ACCCTTCTTCTTGCCACCTTTC	201	XM_010815980
MYF5	F：CTCTGATGGCATGCCTGAATGT	180	NM_174116
MYF5	R：GGCAATCCAGGTTGCTCTGA	180	NM_174116
MYF6	F：TGGACCCCTTCAGCTACAGAC	126	NM_181811
MYF6	R：CTTCCTTGGCAGTTATCACGAG	126	NM_181811
BAX	F：GAGATGAATTGGACAGTAACA	118	NM_173894
BAX	R：TTGAAGTTGCCGTCAGAA	118	NM_173894
BAD	F：CTCAGCAAGCACTGGCTAACA	270	NM_001035459
BAD	R：GTGAAACTCGTCGCTCATCCT	270	NM_001035459
BCL2	F：TTAGCCAGTGCTTGCTGAGAC	86	NM_001166486
BCL2	R：TTAGCCAGTGCTTGCTGAGAC	86	NM_001166486

基因名称 Gene name	引物方向 Primer direction	引物序列 Primer sequence (5′→3′)	产物长度 Products length (bp)	基因登录号 Genbank ID
FoxO1	F-Kpn I-FoxO1	CGGGGTACCGCCACCATGGCCGAAGCGCCCCAGGTG	1998	XM_025000053.1
FoxO1	R-Hind IlI-FoxO1	CCCAAGCTTTCAGCCTGACACCCAGCTGTGTG	1998	XM_025000053.1

Study on the Role of FoxO1 in Regulating the Proliferation, Apoptosis and Differentiation of Bovine Skeletal Muscle Cells

RichHTML

PDF