Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (23): 4772-4780.doi: 10.3864/j.issn.0578-1752.2011.23.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effect of Exogenous L-Phentermine and UV-C on the Accumulation of Rutin Compounds and the Expression of Rutin Biosynthesis Genes in Fagopyrum tartaricum

 SUN  Chao-Xia, HOU  Si-Yu, YANG  Wu-De   

  1. 1.山西农业大学旱作农业工程研究所，山西太谷 030801
  • Received:2011-06-03 Online:2011-12-01 Published:2011-08-31

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Abstract: 【Objective】 The objective of this study is to investigate the content of rutin and the expression of the related genes in response to L-phentermine (Phe) and UV-C treatment, and to provide molecular information for high rutin content buckwheat breeding. 【Method】 Rutin biosynthesis-related genes were cloned from the leaves of F. tataricum by 3′RACE. Rutin content in leaves under Phe treatment (1, 2 and 4 mg?L-1, 1 to 5 d) was compared with that of untreated buckwheat by high performance liquid chromatography (HPLC). The gene expression level was analyzed by qRT-PCR. 【Result】 Three partial CDS of rutin biosynthesis-related genes were cloned. They were chalcone synthase (FtCHS), flavonol synthase-like (FtFLS-like) and flavone 3-hydroxylase (FtF3H). Seedlings were cultured in solution containing Phe (4 mg×L-1) for 1 d, rutin content was two times more than the control (12.55 mg×gFW-1), reached 26.15 mg×gFW-1. The higher rutin content (17.36 mg×gFW-1) was found in leaves at 3 h under UV-C treatment. Differential expression patterns of FtCHS, FtFLS-like and FtF3H were observed in different Phe concentration treatments in leaves of F. tartaricum. Seedlings were cultured in solution containing Phe (1 mg×L-1) for 4 d, FtCHS expression  level was 4.84 fold higher than the control. The higher expression levels of FtF3H and FtFLS-like were observed after 4 and 2 mg×L-1 Phe treatment for 5 d, they were 1.06 and 3.90 fold higher than the control, respectively. UV-C radiation for 1-3 h, the expression      of FtCHS, FtFLS-like and FtF3H was higher than control. In particular, the expression level of FtFLS-like at 1 h under UV-C radiation was 127.71 fold higher than the control. 【Conclusion】 Rutin content could be enhanced to a great extent by Phe treatment and UV-C radiation, and gene expression patterns of rutin biosysthesis-related genes were regulated by UV-C radiation. It can be used to analyze the pathway of rutin biosynthesis. Further study should be focused on the influential factors of rutin metabolic process.

Key words: Fagopyrum tartaricum, rutin, L-phenylalanine, UV-C, gene expression

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