Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (17): 3683-3693.doi: 10.3864/j.issn.0578-1752.2011.17.022

• RESEARCH NOTES • Previous Articles     Next Articles

23S-5S rDNA Sequence Cloning and Analysis of Candidatus Liberibacter africanus and americanus of Citrus Huanglongbing

LIAO  Hui-Hong, LI  Yang-Rui, YANG  Li-Tao, XU  Ning   

  1. 1.广西农业科学院园艺研究所
    2.广西作物遗传改良生物技术重点开放实验室
    3.广西大学亚热带农业生物资源保护与利用国家重点实验室
  • Received:2010-10-25 Revised:2011-01-19 Online:2011-09-01 Published:2011-02-28
  • Contact: Hui-Hong LIAO E-mail:liyr@gxaas.net

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Abstract: 【Objective】 The objective of this study is to clone 23S-5S rDNA sequences of Candidatus Liberibacter africanus and americanus and compare to the corresponding region sequences of Candidatus Liberibacter asiaticus to clarify the ribosomal RNA operons relationships of HLB three species.【Method】The HLB DNA samples from africanus and americanus were amplified by the primers designed according to the 23S-5S rRNA gene conserved sequences of asiaticus. The PCR products were cloned and sequenced. The newly obtained sequences were further identified and analyzed.【Result】Sequences of 3 057 bp in length were obtained from africanus including 23S rRNA gene, cell wall hydrolase pseudogene and 5S rRNA gene. Sequences of 3 033 bp in length were obtained from americanus including 23S rRNA gene, glpK gene and 5S rRNA gene. The gene order is 16S rRNA, tRNAIle, tRNAAla, 23S rRNA, cell wall hydrolase pseudogene, 5S rRNA and tRNAMet for asiaticus. 16S rRNA, tRNAIle, tRNAAla, 23S rRNA, cell wall hydrolase pseudogene, 5S rRNA for africanus. 16S rRNA, tRNAIle, tRNAAla, 23S rRNA, glpK gene, 5s rRNA for americanus. 【Conclusion】The operon arrangement was special for three HLB species. The cell wall hydrolase gene coding in the reverse strand was found between 23S rRNA and 5S rRNA gene in three HLB species. It is a pseudogene for asiaticus and africanus while it is glpK gene for americanus.

Key words: Citrus, HLB, Candidatus Liberibacter africauns, Candidatus Liberibacter americanus, 23S-5S rDNA

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