Abstract:

【Objective】 In order to simplify the Biolog analysis, 9 kinds of characteristic carbon sources selected from the carbon resources in Biolog ECO microplate containing 10 carbon resources and added in Biolog MT microplate to analyse soil microbial cummunity where bacterial wilt of tomato occurred. 【Method】 The optimal concentration of characteristic carbon resources added in Biolog MT microplates was studied with the soil of potted tomato and three tests of verifying the carbon resource concentrations were carried out by using the soils of potted pepper without soil-borne diseases occurrence treated with different fertilization rates and the soils of field tomato and potted pepper with varied incidences of bacterial wilt. 【Result】 The result of carbon resource concentration experiment showed that the OD values of different concentrations of characteristic carbon resources were obviously different. Compared with the OD in ECO microplate, the optimal concentrations in MT microplate were determined. The results of verifying tests indicated that the soil microbial community functional diversity (Shannon index) with different incidences of crop were differentiated by characteristic carbon resources. 【Conclusion】 Therefore, the characteristic carbon resources could be used to differentiate the health soil and disease soil, and the soils with varied incidences of soil-borne diseases. The Biolog analysis can be simplified by using 9 kinds of characteristic carbon resources.

Key words: Biolog, characteristic carbon source, simplifying, soil-borne disease