Abstract:

【Objective】 The aim of this study is to clone a wheat germin-like protein gene induced by Bgt (Blumeria graminis f. sp. tritici), analyse the expression pattern in susceptible and resistant plants and elucidate the molecular mechanism of the gene in resistance to powdery mildew. 【Method】 Gene chip hybridization, in silico cloning and RT-PCR methods were used to clone a wheat germin-like gene induced by Bgt, named as TaGLP5 (GenBank accession number FJ594470). A set of nulli-tetrasomic lines of Chinese Spring were employed to locate the gene. Its expression pattern at transcription level was investigated by quantitative PCR in resistant and susceptible progenies. 【Result】 A wheat germin-like protein, TaGLP5 with a complete ORF was cloned. Phylogenetic assay indicated that this gene differed from the known germin-like proteins from gramineous family. It was located on chromosome 5A. Quantitative PCR showed that the gene was induced by Bgt and higher expression in resistant plants than in susceptible ones before 24 h of inoculation. These will provide an important reference for further study on the gene. 【Conclusion】 A new wheat germin-like protein was obtained in this study. The expression patterns results revealed that TaGLP5 was up-regulated in both resistant and susceptible plants. However, the expression in quantity and time was different. It was speculated that TaGLP5 was involved in wheat defense response to Bgt.

Key words: Blumeria graminis f. sp. tritici(Bgt)')">Blumeria graminis f. sp. tritici(Bgt), germin-like protein(GLP), cloning, expression pattern, chromosome location