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Table of Content

    10 September 2009, Volume 42 Issue 9
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Spatial and Temporal Profiling of DNA Methylation Induced by Drought Stress in Rice#br#
    PAN Ya-jiao,FU Bin-ying,WANG Di,ZHU Ling-hua,LI Zhi-kang
    Scientia Agricultura Sinica. 2009, 42(9):  3009-3018 .  doi:10.3864/j.issn.0578-1752.2009.09.001
    Abstract ( 1391 )   PDF (485KB) ( 1487 )   Save
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    【Objective】 This experiment aimed at study of the relationship between DNA methylation and drought responsiveness in rice by characterizing DNA mathylation in leaves and roots at seedling stages and tillering stages under drought stress for DK106 (drought tolerance introgression line) and IR64 (recurrent parent and drought sensitive line). 【Method】 Methylation sensitive amplified polymorphism (MSAP) and high performance liquid chromatography (HPLC) were used to analyze the DNA methylation level in this study; The differentially methylated DNA fragments induced by drought were isolated from the polyacrylamide gel, cloned and sequenced, then the sequences were blasted at the NCBI website. 【Result】 Results showed that about 20% cytosine of CCGG sequences in rice genome was methylated, and the average level of methylation was increased under drought stress treatment, especially in roots; But the alterations of the DNA methylation state and level induced by drought stress were different between DK106 and IR64, and were also specific at different growth stages and in different tissues. The BLAST results of the differentially methylated DNA sequences showed that the methylation frequency was similar in coding region and non-coding region on the genome. 【Conclusion】 It was revealed that the pattern of DNA methylation under drought was temporal-spatial specific and variety specific, and the change of DNA methylation was related to drought responsiveness.

    Male Sterility and Thermo-Photosensitivity Characteristics of BNS in Wheat#br#
    LI Luo-jiang,RU Zhen-gang,GAO Qing-rong,JIANG Hui,GUO Feng-zhi,WU Shi-wen,SUN Zhe
    Scientia Agricultura Sinica. 2009, 42(9):  3019-3027 .  doi:10.3864/j.issn.0578-1752.2009.09.002
    Abstract ( 1260 )   PDF (390KB) ( 1006 )   Save
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    【Objective】 In order to evaluate the heterosis utilization of BNS in wheat, the fertile performance, thermo-photosensitive characteristics and the fertile conversion were studied with BNS and Bainongaikang 58 in present work.【Method】 The method of time sowing was conducted in autumn as well as the sowing in spring. The temperature and light length were also recorded to study the fertility, dynamics of average daily temperature and light length, and the thermo-photosensitive effect.【Result】 The result showed that the spikes of BNS were light, shag and expanding. The anthers were slim and thin. A little present pollen was lack of activity, and the sterile type of spherical abortion was identified by iodine-treatment. The male-sterile lines exhibited high and stable sterility. The national and international seed setting rate of artificial saturation pollination ranged from 79.64% to 87.22% and 89.89% to 102.10%, respectively, indicating that the pistil was normal. The thermo-sensitive stage of BNS with high sterility (97.57%-100%) was from the differentiating period of floret primordium to pistil and stamen primordium, when the sowing date was earlier than October 17, and the temperature ranged from 7.4℃ to 11.4℃ in this period. The transformation of fertility was detected, with a range of self-seeds rate from 7.71% to 70.15% (National) and 9.41% to 102.50% (International), when the temperature was higher than 11.4℃ in the following sowing dates. Correlation analysis was performed to detect the relationship between sterile degree and temperature. The correlation coefficients (r) ranged from -0.578 to -0.866 (r0.05/0.01=0.532/0.661), which indicated that correlation was significant or extremely significant between sterile degree and temperature. Similar to referenced material Bainongaikang 58, BNS sowed in spring was fertile with the temperature up to 15.9℃ during the differentiating stage of floret primordium to pistil and stamen primordium. During the transforming stage of fertility, the sites of seed setting were mainly on basal and middle spikelets but top ones. Compared with caulis spikes, the male sterility of whereabout spikes was lower. There were differences between transformation of fertility and light length with different sowing dates and stages before heading, but no obvious regularity was found. 【Conclusion】 Above all, BNS was a kind of male-sterile line with thermo-sensitive characteristics, and its sterility was induced by low temperature with the assistant of light. With this promising male-sterile line, the production of hybrid seed and seed of male-sterile line in local areas could be conducted easily with autumn sowing and spring sowing.

    Reducing the Maize Amylopectin Content Through Anti-Sense Manipulation#br#
    GUAN Shu-yan,WANG Pei-wu,LIU Guang-na,LIU Hui-jing,ZHAO Li-na
    Scientia Agricultura Sinica. 2009, 42(9):  3028-3035 .  doi:10.3864/j.issn.0578-1752.2009.09.003
    Abstract ( 1204 )   PDF (548KB) ( 1189 )   Save
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    【Objective】 In order to control the biosynthesis process of maize starch and to develop transgenic maize with high amylase starch content. 【Method】 RT-PCR was employed to clone the maize starch branching enzyme gene sbe2a and high efficient anti-sense expression vector was constructed based on plant expression plasmid pWGLL. Then the vector was introduced into maize inbred line Tie7922 by pollen tube pathway. 【Result】 Four transgenic plants were obtained. PCR amplification and Southern blotting hybridization proved that the sbe2a had integrated into maize genome. RT-PCR and enzyme activity results showed that both mRNA level of sbe2a and activity of starch branching enzyme decreased significantly in the transgenic plants than that in the wild type plants and the maximum decrease level of enzyme activity was 79.4%. However the total starch content had no significant difference between the transgenic plants and the wild type plants, while the content of amylose starch increased by 84.3% compared to the wild type plants. 【Conclusion】 Anti-sense RNA is an efficient gene silencing method and can be used effectively in the production of high-amylose maize by silencing endogenesis gene sbe2a.

    Genetic Analysis of Major QTLs and Epistasis Interaction for Yield and Fiber Quality in Upland Cotton#br#

    LIN Zhong-xu,FENG Chang-hui,GUO Xiao-ping,ZHANG Xian-long

    Scientia Agricultura Sinica. 2009, 42(9):  3036-3047 .  doi:10.3864/j.issn.0578-1752.2009.09.004
    Abstract ( 1230 )   PDF (564KB) ( 954 )   Save
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    【Objective】 The aim of this investigation was to better understand the genetic base of yield and fiber quality traits in upland cotton. Major quantitative trait loci (QTL) associated with yield and fiber quality and epistasis interaction in controlling these traits were mapped using a genetic linkage map which comprised 471 loci, spanned 3 070.2 cM and covered 65.88% of the whole cotton genome. 【Method】 An F2:3 population from DH962 × Jimian5 was used to evaluate yield and fiber quality. Major QTLs were identified by composite interval mapping method on WinQTLCartV2.5. Two-way digenic interactions analysis was conducted using EPISTACY. 【Result】 Nine major QTLs were detected for six yield traits and five for fiber quality traits. No QTLs were detected for fiber uniformity ratio and fiber strength under the significant threshold LOD scores. A total of 75 significant digenic interactions were detected, most of them were non-QTL × non-QTL interaction; most of the interaction types were additive × dominance interaction and dominance × additive interaction. Many digenic interactions were detected for lint percentage and fiber length, and only few for boll number and lint index. One common major QTL for SCW, LW and SI was detected on LG1. Common digenic interactions were also found for some correlated traits: one for SCW, LW and MV; four for LP and FL. 【Conclusion】 Except for major QTLs, epistasis played an important role in the genetic basis of yield and fiber traits in upland cotton. The low effect of major QTLs and the digenic interactions will make it more difficult and more complex for maker-assisted selection in cotton breeding. Correlated traits were controlled by same QTLs/digenic interactions, which will be helpful to select these traits simultaneously.

    Heterosis and Genetic Analysis of Oil Content in Peanut Using Mixed Model of Major Gene and Polygene#br#
    CHEN Si-long,LI Yu-rong,CHENG Zeng-shu,LIAO Bo-shou,LEI Yong,LIU Ji-sheng
    Scientia Agricultura Sinica. 2009, 42(9):  3048-3057 .  doi:10.3864/j.issn.0578-1752.2009.09.005
    Abstract ( 1260 )   PDF (349KB) ( 1577 )   Save
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    【Objective】 Oil content is an important quantitative trait of peanut. High oil content has became a major target of genetic improvement on peanut cultivar. Heterosis and genetic analysis of oil content will provide a very important guidance for breeding of high oil peanut cultivar. 【Method】 The method of joint segregation analysis of multiple generations with P1, P2, F1 and F2 of major gene plus polygene mixed inheritance model was used to analyze the inheritance of oil content in peanut cultivars. Four basic populations (P1, P2, F1 and F2) from four peanut crosses respectively were made for analysis of the genetic model. The female parent in each cross was progeny of distant hybridization with a high oil gene pool of peanut wild species. The male parents were peanut cultivars with low oil content. 【Result】 The results indicated that the heterosis of oil content was showed in F1 with significant different degree among four crosses, and the mid-parents heterosis were from 1.4% to 9.4% respectively. The differences of oil content genetic characteristcs among four crosses were obvious. The frequency distributions of oil content in F2 populations derived from two crosses SW9721-3×Te21 and SW9721-12×Puhua22 showed the characteristics of mixture normal distribution, which indicated that inheritance of oil content followed the major gene plus polygene model. Results showed that genetic model D-0 was the most fitted genetic model for the trait. In other words, oil content was controlled by one major gene with additive-dominant effects plus polygenes with additive-dominant-epistasis effects. The major gene heritabilities in F2 were 47.51% and 45.00%, respectively, and polygene heritabilities were 22.75% and 18.72%, respectively. The frequency distributions of oil content in F2 populations derived from SW9721-23×95-3 and SW9721-38×Luhua11 showed the characteristics of normal distribution, which indicated that inheritance of oil content followed the polygene model. Results showed that genetic model C-0 was the most fitted genetic model for the trait. In other words, oil content was controlled by polygenes with additive-dominant-epistasis effects. The polygene heritabilities in F2 were 66.51% and 66.09%, respectively. 【Conclusion】 The heterosis and transgressive segregation of oil content commonly existed in hybrid progenies of peanut. The genetic effects of genes for oil content traits were significantly different in magintude, some with major gene character. Oil content in peanut was controlled by additive gene effects. And therefore, in high oil peanut breeding for cultivars improvement, some strains with high oil content may be selected through single directional selection.

    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY
    Differences of Root Traits in Conventional Indica Rice Cultivars with Different Types of Sink-Potential #br#
    DONG Gui-chun,DONG Yan-ping,ZHANG Biao,ZHOU Juan,LI Jin-qian,YU Xiao-feng,TIAN Hao,WANG Yu-long
    Scientia Agricultura Sinica. 2009, 42(9):  3058-3066 .  doi:10.3864/j.issn.0578-1752.2009.09.006
    Abstract ( 1329 )   PDF (340KB) ( 771 )   Save
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    【Objective】 The goal of the research is to analyze the characteristics of root traits in conventional indica rice cultivars with large sink potential (SP). 【Method】 88 and 122 conventional indica rice cultivars were solution-cultured in 2001 and 2002, respectively. Dry matter weight (including root system, culm and sheath, leaves, panicle), root traits, root physiological activity, yield and its components were measured. The tested rice cultivars were classified into 6 types (i.e. A, B, C, D, E and F, A was the lowest, and F was the highest) based on their SP level by the MinSSw method, to study their differences of root traits, root physiological activity. 【Result】 Difference of SP of the cultivars used in this study was very large, the averages SP of A, B, C, D, E, and F were 426.37, 642.53, 770.96, 903.73, 1 064.32, 1 213.90 g?m-2 in 2001, and 359.36, 574.11, 764.98, 962.43, 1 200.11, 1 455.59 g?m-2 in 2002, respectively. The number of adventitious roots per unit area, total length of adventitious roots per unit area and root dry weight per unit area in large SP type variety was higher than that in small SP type variety, no significant differences of traits per adventitious and per unit root dry weight between different SP type cultivars were observed. Polynomial regression analysis showed that SP level was significantly influenced the length of adventitious roots per unit area, root dry weight per unit area, ratio of shoot to root at heading stages, root activity per unit area, and the determinant coefficient ranged from 0.423-0.578. Large SP could be reached by increasing the length of adventitious roots per unit area, dry weight per unit area, ratio of shoot to root at heading stage, root activity per unit area. 【Conclusion】 Root traits per unit area in large SP type variety are higher than that in small SP type variety, larg SP could be reached by increasing the length of adventitious roots per unit area, root dry weight per unit area, ratio of shoot to root at heading stages, root activity per unit area.

    Effects of Precise and Quantitative Cultivation on Plant Type and Yield of Rice in High Altitude and Cold Ecological Area#br#
    LI Jing-hong,LI Gang-hua,ZHANG Ying-gui,LUO Qi-rong,YANG Cong-dang,WANG Shao-hua,LIU Zheng-hui,WANG Qiang-sheng,DING Yan-feng
    Scientia Agricultura Sinica. 2009, 42(9):  3067-3077 .  doi:10.3864/j.issn.0578-1752.2009.09.007
    Abstract ( 1046 )   PDF (281KB) ( 1144 )   Save
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    【Objective】 Correlation between plant type indexes and yield components of rice as well as precise and quantitative cultivation on its mechanism of increase production were studied in special ecology area under high-altitude and cold. 【Method】 By using japonica rice landraces Dabaigu and new varieties Lijing 10 as test materials to analyze the correlation between plant type indexes and yield components at heading stage and research on growth and development as well as ecological and physiological characteristics of rice. The target of main attack on increasing production was studied to compare the precise and quantitative cultivation and conventional cultivation and the effects of precise and quantitative cultivation on plant type and yield of rice in high altitude and cold ecological area were analyzed. 【Result】 The results showed that there were differences in characteristics of plant type and grain yield components such as effective panicles, total number of spikelets among different populations. The notable characteristics of high-yield group were that the length of top three leaves were longer. The angles of top three leaves were smaller, the specific leaf weight was high at heading stage and closely related to the traits of leaves, stems and panicles. The length of internode on stem distributed reasonably, bottom internode was shorter while top internode was longer. A significantly positive correlation was observed between first branch numbers, second branch numbers and grain yield. 【Conclusion】 Enlarged sink (increased total number of spikelets) and the promoted sources (increased the leaf area index after heading stage and the specific leaf weight) were the main reasons of increased rice yield. It is concluded that synchronizing nitrogen and water application in precise and quantitative cultivation and organ formation in high-yield rice, increasing the leaf area index and simultaneously promoting total number of spikelets, rice yield could significantly increase rice yield in high-altitude eco-areas.

    Comparison of Nitrogen Metabolism and Activities of the Related Enzymes During Grain Filling Stage Among Wheat Cultivars Differing in Protein Content #br#
    LI Jian-min,WANG Zhen-lin,YIN Yan-ping,GAO Rong-qi,LI Sheng-fu,YAN Su-hui,YU An-ling
    Scientia Agricultura Sinica. 2009, 42(9):  3078-3086 .  doi:10.3864/j.issn.0578-1752.2009.09.008
    Abstract ( 1292 )   PDF (278KB) ( 962 )   Save
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    【Objective】 The present study was carried out to characterize the nitrogen metabolism and activities of the related enzymes during grain filling stage among wheat cultivars differing in protein content. This information will be helpful to elucidate the effects of nitrogen form in source and sink and activities of the related enzymes on the protein content in grains of wheat and will be valuable to wheat cultivation for high quality. 【Method】 Two cultivars from each of the three types of cultivars, the high-, middle- and low-protein content cultivars, were grown in the field of the experimental station of Shandong Agricultural University in Tai’an. The contents of nitrate nitrogen, ammonia nitrogen and activities of enzymes relating to nitrogen metabolism were tested. 【Result】 The protein content of wheat grains showed the change pattern of “high-low-high” during grain filling stage and the protein accumulation in the mature grains was in the order of the high- >middle- > low-protein content cultivars. The content of nitrate nitrogen in grain decreased gradually as grain developed, and at maturity, it was in the order of the high- > middle- > low-protein content cultivars. The change of ammonia nitrogen content in flag leaves and grains was similar to that of nitrate nitrogen content in grains. But the ammonia nitrogen content at maturity varied with cultivars. The activities of nitrate reductase (NR) decreased as grains developed, especially, for low-protein content cultivars, the NR activity of which was lower than that of high- and middle-protein content cultivars. The activities of glutamine synthetase (GS) in grains and flag leaves decreased with grains developing in both high- and middle-content cultivars. As for low-protein content cultivars, the change of GS activities in grains was different from that in flag leaves, the later remained the higher value as compared with that of both high- and middle- protein content cultivars.【Conclusion】 It was found that the content of nitrate nitrogen in grains has a significantly positive correlation with activities of NR in flag leaves or GS in grains, also the content of ammonia nitrogen in grains still has a significantly positive correlation with activities of NR in flag leaves or GS in grains and flag leaves. The high- protein content cultivars have stronger ability of supplying nitrate nitrogen for sink from source than middle- and low-protein content cultivars. But low protein content cultivars can make more efficient use of nitrate nitrogen. The high-protein content cultivars with high activities of NR and GS have a stronger capacity of nitrogen assimilation than that of middle- and low-protein content cultivars. The sufficient supply of substrate for protein synthesis is helpful for protein accumulation in wheat grains.

    Spatial Variation Analysis of Wheat Grain Quality Based on Model and GIS#br#
    HUANG Fen,ZHU Yan,JIANG Dong,JING Qi,CAO Wei-xing
    Scientia Agricultura Sinica. 2009, 42(9):  3087-3095 .  doi:10.3864/j.issn.0578-1752.2009.09.009
    Abstract ( 1136 )   PDF (449KB) ( 865 )   Save
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    【Objective】 The aim of this study is to explore the method of simulating the grain quality index and analyzing the spatial variation characteristics based on GIS and wheat quality index prediction model. 【Method】 Firstly, literature data with five varieties at five eco-sites were used for the evaluation of grain quality model at site scale. Secondly, based on the weather data sets of 2000-2003 at 40 eco-sites in Jiangsu province and experimental data from five eco-sites and six wheat cultivars, three main wheat grain quality indices at regional scale were calculated with two methods such as ‘calculate first, interpolate later’ (CI) and ‘interpolate first, calculate later’ (IC). Finally, the spatial variation characteristics of three wheat grain quality indices in Jiangsu province were analyzed, and three spatial distribution raster maps for grain protein content, wet gluten content and sedimentation were made based on the geo-statistics and GIS. 【Result】 The IC was suggested to be preferable for up scaling the grain quality model, with the RMSE less than 20% between simulated and observed values for three quality indices. The spatial autocorrelation of grain protein content, wet gluten content and sedimentation under research region was significant within the 7.16 km variation range, and the anisotropic structure varies more evidently in longitudinal and latitudinal directions. The spatial raster maps could show the distribution and the variation trend of regional grain quality effectively. 【Conclusion】 The result indicated that the IC based simulation on regional spatial variation of wheat grain quality is feasible. This study might provide reference for analysis of ecological variation on crop quality.

    Effects of Nitrogen Top-Dressing at Jointing Stage on Pasting Properties of Spring and Autumn Sown Waxy Maize Flour #br#
    LU Da-lei,JING Li-quan,WANG De-cheng,HAN qing,GUO Huan-fen,ZHAO Jiu-ran,LU Wei-ping
    Scientia Agricultura Sinica. 2009, 42(9):  3096-3103 .  doi:10.3864/j.issn.0578-1752.2009.09.010
    Abstract ( 1146 )   PDF (327KB) ( 754 )   Save
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    【Objective】 The aim of the study is to understand the effects of nitrogen top-dressing amount at jointing stage on flour pasting properties of spring and autumn sown waxy maize varieties. 【Method】 Research was carried out at the Experiment Farm of Yangzhou University in 2008. Three varieties (Suyunuo 5, Kennian 1 and Suyunuo 1, the controls of Chinese waxy maize in regional test) were used in the study. The effects of nitrogen top-dressing amount (0, 150 and 300 kg?hm-2) at jointing stage on flour pasting properties of spring and autumn sown waxy maize were clarified by Rapid Visco Analyzer (RVA). 【Result】 Waxy maize flour pasting properties were significantly affected by the single factor and interactions of nitrogen top-dressing amount at jointing stage, sown dates and varieties. In general, the flour present higher peak viscosity, breakdown, trough viscosity and final viscosity, shorter peak time and lower pasting temperature when nitrogen top-dressing amount was 150 kg?hm-2 and in autumn-sown treatment, and those RVA characteristics were better for Suyunuo 5. Significantly positive correlations were observed among peak viscosity, trough viscosity, breakdown and final viscosity, and those four parameters were negatively correlated to peak time and pasting temperature. In addition, peak time was observed positively correlated to pasting temperature, and setback was positively correlated to final viscosity. 【Conclusion】Considering the pasting properties in different treatments, the autumn sown waxy maize varieties and nitrogen top-dressing amount at jointing stage was suitable (150 kg?hm-2 in this experiment) and could got the flour with higher peak viscosity and breakdown, shorter peak time and lower pasting temperature.

    PLANT PROTECTION
    Cloning and Chromosome Mapping of a Germin-Like Protein Gene in Wheat and Its Expression in Response to Infection with Wheat Powdery Mildew#br#
    WANG Jun-mei,SUN Yan-fei,LIU Hong-yan,KANG Zhen-sheng,XU Hong-ming
    Scientia Agricultura Sinica. 2009, 42(9):  3104-3111 .  doi:10.3864/j.issn.0578-1752.2009.09.011
    Abstract ( 1016 )   PDF (394KB) ( 1149 )   Save
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    【Objective】 The aim of this study is to clone a wheat germin-like protein gene induced by Bgt (Blumeria graminis f. sp. tritici), analyse the expression pattern in susceptible and resistant plants and elucidate the molecular mechanism of the gene in resistance to powdery mildew. 【Method】 Gene chip hybridization, in silico cloning and RT-PCR methods were used to clone a wheat germin-like gene induced by Bgt, named as TaGLP5 (GenBank accession number FJ594470). A set of nulli-tetrasomic lines of Chinese Spring were employed to locate the gene. Its expression pattern at transcription level was investigated by quantitative PCR in resistant and susceptible progenies. 【Result】 A wheat germin-like protein, TaGLP5 with a complete ORF was cloned. Phylogenetic assay indicated that this gene differed from the known germin-like proteins from gramineous family. It was located on chromosome 5A. Quantitative PCR showed that the gene was induced by Bgt and higher expression in resistant plants than in susceptible ones before 24 h of inoculation. These will provide an important reference for further study on the gene. 【Conclusion】 A new wheat germin-like protein was obtained in this study. The expression patterns results revealed that TaGLP5 was up-regulated in both resistant and susceptible plants. However, the expression in quantity and time was different. It was speculated that TaGLP5 was involved in wheat defense response to Bgt.

    Development of Genomic SSR Markers for Phytophthora sojae#br#
    XU Jing-jing,WANG Xiao-ming,DUAN Can-xing,WU Xiao-fei,ZHU Zhen-dong
    Scientia Agricultura Sinica. 2009, 42(9):  3112-3122 .  doi:10.3864/j.issn.0578-1752.2009.09.01