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Table of Content

    10 September 2009, Volume 42 Issue 9
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Spatial and Temporal Profiling of DNA Methylation Induced by Drought Stress in Rice#br#
    PAN Ya-jiao,FU Bin-ying,WANG Di,ZHU Ling-hua,LI Zhi-kang
    Scientia Agricultura Sinica. 2009, 42(9):  3009-3018 .  doi:10.3864/j.issn.0578-1752.2009.09.001
    Abstract ( 1153 )   PDF (485KB) ( 1265 )   Save
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    【Objective】 This experiment aimed at study of the relationship between DNA methylation and drought responsiveness in rice by characterizing DNA mathylation in leaves and roots at seedling stages and tillering stages under drought stress for DK106 (drought tolerance introgression line) and IR64 (recurrent parent and drought sensitive line). 【Method】 Methylation sensitive amplified polymorphism (MSAP) and high performance liquid chromatography (HPLC) were used to analyze the DNA methylation level in this study; The differentially methylated DNA fragments induced by drought were isolated from the polyacrylamide gel, cloned and sequenced, then the sequences were blasted at the NCBI website. 【Result】 Results showed that about 20% cytosine of CCGG sequences in rice genome was methylated, and the average level of methylation was increased under drought stress treatment, especially in roots; But the alterations of the DNA methylation state and level induced by drought stress were different between DK106 and IR64, and were also specific at different growth stages and in different tissues. The BLAST results of the differentially methylated DNA sequences showed that the methylation frequency was similar in coding region and non-coding region on the genome. 【Conclusion】 It was revealed that the pattern of DNA methylation under drought was temporal-spatial specific and variety specific, and the change of DNA methylation was related to drought responsiveness.

    Male Sterility and Thermo-Photosensitivity Characteristics of BNS in Wheat#br#
    LI Luo-jiang,RU Zhen-gang,GAO Qing-rong,JIANG Hui,GUO Feng-zhi,WU Shi-wen,SUN Zhe
    Scientia Agricultura Sinica. 2009, 42(9):  3019-3027 .  doi:10.3864/j.issn.0578-1752.2009.09.002
    Abstract ( 1076 )   PDF (390KB) ( 920 )   Save
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    【Objective】 In order to evaluate the heterosis utilization of BNS in wheat, the fertile performance, thermo-photosensitive characteristics and the fertile conversion were studied with BNS and Bainongaikang 58 in present work.【Method】 The method of time sowing was conducted in autumn as well as the sowing in spring. The temperature and light length were also recorded to study the fertility, dynamics of average daily temperature and light length, and the thermo-photosensitive effect.【Result】 The result showed that the spikes of BNS were light, shag and expanding. The anthers were slim and thin. A little present pollen was lack of activity, and the sterile type of spherical abortion was identified by iodine-treatment. The male-sterile lines exhibited high and stable sterility. The national and international seed setting rate of artificial saturation pollination ranged from 79.64% to 87.22% and 89.89% to 102.10%, respectively, indicating that the pistil was normal. The thermo-sensitive stage of BNS with high sterility (97.57%-100%) was from the differentiating period of floret primordium to pistil and stamen primordium, when the sowing date was earlier than October 17, and the temperature ranged from 7.4℃ to 11.4℃ in this period. The transformation of fertility was detected, with a range of self-seeds rate from 7.71% to 70.15% (National) and 9.41% to 102.50% (International), when the temperature was higher than 11.4℃ in the following sowing dates. Correlation analysis was performed to detect the relationship between sterile degree and temperature. The correlation coefficients (r) ranged from -0.578 to -0.866 (r0.05/0.01=0.532/0.661), which indicated that correlation was significant or extremely significant between sterile degree and temperature. Similar to referenced material Bainongaikang 58, BNS sowed in spring was fertile with the temperature up to 15.9℃ during the differentiating stage of floret primordium to pistil and stamen primordium. During the transforming stage of fertility, the sites of seed setting were mainly on basal and middle spikelets but top ones. Compared with caulis spikes, the male sterility of whereabout spikes was lower. There were differences between transformation of fertility and light length with different sowing dates and stages before heading, but no obvious regularity was found. 【Conclusion】 Above all, BNS was a kind of male-sterile line with thermo-sensitive characteristics, and its sterility was induced by low temperature with the assistant of light. With this promising male-sterile line, the production of hybrid seed and seed of male-sterile line in local areas could be conducted easily with autumn sowing and spring sowing.

    Reducing the Maize Amylopectin Content Through Anti-Sense Manipulation#br#
    GUAN Shu-yan,WANG Pei-wu,LIU Guang-na,LIU Hui-jing,ZHAO Li-na
    Scientia Agricultura Sinica. 2009, 42(9):  3028-3035 .  doi:10.3864/j.issn.0578-1752.2009.09.003
    Abstract ( 949 )   PDF (548KB) ( 977 )   Save
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    【Objective】 In order to control the biosynthesis process of maize starch and to develop transgenic maize with high amylase starch content. 【Method】 RT-PCR was employed to clone the maize starch branching enzyme gene sbe2a and high efficient anti-sense expression vector was constructed based on plant expression plasmid pWGLL. Then the vector was introduced into maize inbred line Tie7922 by pollen tube pathway. 【Result】 Four transgenic plants were obtained. PCR amplification and Southern blotting hybridization proved that the sbe2a had integrated into maize genome. RT-PCR and enzyme activity results showed that both mRNA level of sbe2a and activity of starch branching enzyme decreased significantly in the transgenic plants than that in the wild type plants and the maximum decrease level of enzyme activity was 79.4%. However the total starch content had no significant difference between the transgenic plants and the wild type plants, while the content of amylose starch increased by 84.3% compared to the wild type plants. 【Conclusion】 Anti-sense RNA is an efficient gene silencing method and can be used effectively in the production of high-amylose maize by silencing endogenesis gene sbe2a.

    Genetic Analysis of Major QTLs and Epistasis Interaction for Yield and Fiber Quality in Upland Cotton#br#

    LIN Zhong-xu,FENG Chang-hui,GUO Xiao-ping,ZHANG Xian-long

    Scientia Agricultura Sinica. 2009, 42(9):  3036-3047 .  doi:10.3864/j.issn.0578-1752.2009.09.004
    Abstract ( 1107 )   PDF (564KB) ( 884 )   Save
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    【Objective】 The aim of this investigation was to better understand the genetic base of yield and fiber quality traits in upland cotton. Major quantitative trait loci (QTL) associated with yield and fiber quality and epistasis interaction in controlling these traits were mapped using a genetic linkage map which comprised 471 loci, spanned 3 070.2 cM and covered 65.88% of the whole cotton genome. 【Method】 An F2:3 population from DH962 × Jimian5 was used to evaluate yield and fiber quality. Major QTLs were identified by composite interval mapping method on WinQTLCartV2.5. Two-way digenic interactions analysis was conducted using EPISTACY. 【Result】 Nine major QTLs were detected for six yield traits and five for fiber quality traits. No QTLs were detected for fiber uniformity ratio and fiber strength under the significant threshold LOD scores. A total of 75 significant digenic interactions were detected, most of them were non-QTL × non-QTL interaction; most of the interaction types were additive × dominance interaction and dominance × additive interaction. Many digenic interactions were detected for lint percentage and fiber length, and only few for boll number and lint index. One common major QTL for SCW, LW and SI was detected on LG1. Common digenic interactions were also found for some correlated traits: one for SCW, LW and MV; four for LP and FL. 【Conclusion】 Except for major QTLs, epistasis played an important role in the genetic basis of yield and fiber traits in upland cotton. The low effect of major QTLs and the digenic interactions will make it more difficult and more complex for maker-assisted selection in cotton breeding. Correlated traits were controlled by same QTLs/digenic interactions, which will be helpful to select these traits simultaneously.

    Heterosis and Genetic Analysis of Oil Content in Peanut Using Mixed Model of Major Gene and Polygene#br#
    CHEN Si-long,LI Yu-rong,CHENG Zeng-shu,LIAO Bo-shou,LEI Yong,LIU Ji-sheng
    Scientia Agricultura Sinica. 2009, 42(9):  3048-3057 .  doi:10.3864/j.issn.0578-1752.2009.09.005
    Abstract ( 1075 )   PDF (349KB) ( 1144 )   Save
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    【Objective】 Oil content is an important quantitative trait of peanut. High oil content has became a major target of genetic improvement on peanut cultivar. Heterosis and genetic analysis of oil content will provide a very important guidance for breeding of high oil peanut cultivar. 【Method】 The method of joint segregation analysis of multiple generations with P1, P2, F1 and F2 of major gene plus polygene mixed inheritance model was used to analyze the inheritance of oil content in peanut cultivars. Four basic populations (P1, P2, F1 and F2) from four peanut crosses respectively were made for analysis of the genetic model. The female parent in each cross was progeny of distant hybridization with a high oil gene pool of peanut wild species. The male parents were peanut cultivars with low oil content. 【Result】 The results indicated that the heterosis of oil content was showed in F1 with significant different degree among four crosses, and the mid-parents heterosis were from 1.4% to 9.4% respectively. The differences of oil content genetic characteristcs among four crosses were obvious. The frequency distributions of oil content in F2 populations derived from two crosses SW9721-3×Te21 and SW9721-12×Puhua22 showed the characteristics of mixture normal distribution, which indicated that inheritance of oil content followed the major gene plus polygene model. Results showed that genetic model D-0 was the most fitted genetic model for the trait. In other words, oil content was controlled by one major gene with additive-dominant effects plus polygenes with additive-dominant-epistasis effects. The major gene heritabilities in F2 were 47.51% and 45.00%, respectively, and polygene heritabilities were 22.75% and 18.72%, respectively. The frequency distributions of oil content in F2 populations derived from SW9721-23×95-3 and SW9721-38×Luhua11 showed the characteristics of normal distribution, which indicated that inheritance of oil content followed the polygene model. Results showed that genetic model C-0 was the most fitted genetic model for the trait. In other words, oil content was controlled by polygenes with additive-dominant-epistasis effects. The polygene heritabilities in F2 were 66.51% and 66.09%, respectively. 【Conclusion】 The heterosis and transgressive segregation of oil content commonly existed in hybrid progenies of peanut. The genetic effects of genes for oil content traits were significantly different in magintude, some with major gene character. Oil content in peanut was controlled by additive gene effects. And therefore, in high oil peanut breeding for cultivars improvement, some strains with high oil content may be selected through single directional selection.

    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY
    Differences of Root Traits in Conventional Indica Rice Cultivars with Different Types of Sink-Potential #br#
    DONG Gui-chun,DONG Yan-ping,ZHANG Biao,ZHOU Juan,LI Jin-qian,YU Xiao-feng,TIAN Hao,WANG Yu-long
    Scientia Agricultura Sinica. 2009, 42(9):  3058-3066 .  doi:10.3864/j.issn.0578-1752.2009.09.006
    Abstract ( 1170 )   PDF (340KB) ( 738 )   Save
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    【Objective】 The goal of the research is to analyze the characteristics of root traits in conventional indica rice cultivars with large sink potential (SP). 【Method】 88 and 122 conventional indica rice cultivars were solution-cultured in 2001 and 2002, respectively. Dry matter weight (including root system, culm and sheath, leaves, panicle), root traits, root physiological activity, yield and its components were measured. The tested rice cultivars were classified into 6 types (i.e. A, B, C, D, E and F, A was the lowest, and F was the highest) based on their SP level by the MinSSw method, to study their differences of root traits, root physiological activity. 【Result】 Difference of SP of the cultivars used in this study was very large, the averages SP of A, B, C, D, E, and F were 426.37, 642.53, 770.96, 903.73, 1 064.32, 1 213.90 g?m-2 in 2001, and 359.36, 574.11, 764.98, 962.43, 1 200.11, 1 455.59 g?m-2 in 2002, respectively. The number of adventitious roots per unit area, total length of adventitious roots per unit area and root dry weight per unit area in large SP type variety was higher than that in small SP type variety, no significant differences of traits per adventitious and per unit root dry weight between different SP type cultivars were observed. Polynomial regression analysis showed that SP level was significantly influenced the length of adventitious roots per unit area, root dry weight per unit area, ratio of shoot to root at heading stages, root activity per unit area, and the determinant coefficient ranged from 0.423-0.578. Large SP could be reached by increasing the length of adventitious roots per unit area, dry weight per unit area, ratio of shoot to root at heading stage, root activity per unit area. 【Conclusion】 Root traits per unit area in large SP type variety are higher than that in small SP type variety, larg SP could be reached by increasing the length of adventitious roots per unit area, root dry weight per unit area, ratio of shoot to root at heading stages, root activity per unit area.

    Effects of Precise and Quantitative Cultivation on Plant Type and Yield of Rice in High Altitude and Cold Ecological Area#br#
    LI Jing-hong,LI Gang-hua,ZHANG Ying-gui,LUO Qi-rong,YANG Cong-dang,WANG Shao-hua,LIU Zheng-hui,WANG Qiang-sheng,DING Yan-feng
    Scientia Agricultura Sinica. 2009, 42(9):  3067-3077 .  doi:10.3864/j.issn.0578-1752.2009.09.007
    Abstract ( 934 )   PDF (281KB) ( 988 )   Save
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    【Objective】 Correlation between plant type indexes and yield components of rice as well as precise and quantitative cultivation on its mechanism of increase production were studied in special ecology area under high-altitude and cold. 【Method】 By using japonica rice landraces Dabaigu and new varieties Lijing 10 as test materials to analyze the correlation between plant type indexes and yield components at heading stage and research on growth and development as well as ecological and physiological characteristics of rice. The target of main attack on increasing production was studied to compare the precise and quantitative cultivation and conventional cultivation and the effects of precise and quantitative cultivation on plant type and yield of rice in high altitude and cold ecological area were analyzed. 【Result】 The results showed that there were differences in characteristics of plant type and grain yield components such as effective panicles, total number of spikelets among different populations. The notable characteristics of high-yield group were that the length of top three leaves were longer. The angles of top three leaves were smaller, the specific leaf weight was high at heading stage and closely related to the traits of leaves, stems and panicles. The length of internode on stem distributed reasonably, bottom internode was shorter while top internode was longer. A significantly positive correlation was observed between first branch numbers, second branch numbers and grain yield. 【Conclusion】 Enlarged sink (increased total number of spikelets) and the promoted sources (increased the leaf area index after heading stage and the specific leaf weight) were the main reasons of increased rice yield. It is concluded that synchronizing nitrogen and water application in precise and quantitative cultivation and organ formation in high-yield rice, increasing the leaf area index and simultaneously promoting total number of spikelets, rice yield could significantly increase rice yield in high-altitude eco-areas.

    Comparison of Nitrogen Metabolism and Activities of the Related Enzymes During Grain Filling Stage Among Wheat Cultivars Differing in Protein Content #br#
    LI Jian-min,WANG Zhen-lin,YIN Yan-ping,GAO Rong-qi,LI Sheng-fu,YAN Su-hui,YU An-ling
    Scientia Agricultura Sinica. 2009, 42(9):  3078-3086 .  doi:10.3864/j.issn.0578-1752.2009.09.008
    Abstract ( 1123 )   PDF (278KB) ( 791 )   Save
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    【Objective】 The present study was carried out to characterize the nitrogen metabolism and activities of the related enzymes during grain filling stage among wheat cultivars differing in protein content. This information will be helpful to elucidate the effects of nitrogen form in source and sink and activities of the related enzymes on the protein content in grains of wheat and will be valuable to wheat cultivation for high quality. 【Method】 Two cultivars from each of the three types of cultivars, the high-, middle- and low-protein content cultivars, were grown in the field of the experimental station of Shandong Agricultural University in Tai’an. The contents of nitrate nitrogen, ammonia nitrogen and activities of enzymes relating to nitrogen metabolism were tested. 【Result】 The protein content of wheat grains showed the change pattern of “high-low-high” during grain filling stage and the protein accumulation in the mature grains was in the order of the high- >middle- > low-protein content cultivars. The content of nitrate nitrogen in grain decreased gradually as grain developed, and at maturity, it was in the order of the high- > middle- > low-protein content cultivars. The change of ammonia nitrogen content in flag leaves and grains was similar to that of nitrate nitrogen content in grains. But the ammonia nitrogen content at maturity varied with cultivars. The activities of nitrate reductase (NR) decreased as grains developed, especially, for low-protein content cultivars, the NR activity of which was lower than that of high- and middle-protein content cultivars. The activities of glutamine synthetase (GS) in grains and flag leaves decreased with grains developing in both high- and middle-content cultivars. As for low-protein content cultivars, the change of GS activities in grains was different from that in flag leaves, the later remained the higher value as compared with that of both high- and middle- protein content cultivars.【Conclusion】 It was found that the content of nitrate nitrogen in grains has a significantly positive correlation with activities of NR in flag leaves or GS in grains, also the content of ammonia nitrogen in grains still has a significantly positive correlation with activities of NR in flag leaves or GS in grains and flag leaves. The high- protein content cultivars have stronger ability of supplying nitrate nitrogen for sink from source than middle- and low-protein content cultivars. But low protein content cultivars can make more efficient use of nitrate nitrogen. The high-protein content cultivars with high activities of NR and GS have a stronger capacity of nitrogen assimilation than that of middle- and low-protein content cultivars. The sufficient supply of substrate for protein synthesis is helpful for protein accumulation in wheat grains.

    Spatial Variation Analysis of Wheat Grain Quality Based on Model and GIS#br#
    HUANG Fen,ZHU Yan,JIANG Dong,JING Qi,CAO Wei-xing
    Scientia Agricultura Sinica. 2009, 42(9):  3087-3095 .  doi:10.3864/j.issn.0578-1752.2009.09.009
    Abstract ( 981 )   PDF (449KB) ( 833 )   Save
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    【Objective】 The aim of this study is to explore the method of simulating the grain quality index and analyzing the spatial variation characteristics based on GIS and wheat quality index prediction model. 【Method】 Firstly, literature data with five varieties at five eco-sites were used for the evaluation of grain quality model at site scale. Secondly, based on the weather data sets of 2000-2003 at 40 eco-sites in Jiangsu province and experimental data from five eco-sites and six wheat cultivars, three main wheat grain quality indices at regional scale were calculated with two methods such as ‘calculate first, interpolate later’ (CI) and ‘interpolate first, calculate later’ (IC). Finally, the spatial variation characteristics of three wheat grain quality indices in Jiangsu province were analyzed, and three spatial distribution raster maps for grain protein content, wet gluten content and sedimentation were made based on the geo-statistics and GIS. 【Result】 The IC was suggested to be preferable for up scaling the grain quality model, with the RMSE less than 20% between simulated and observed values for three quality indices. The spatial autocorrelation of grain protein content, wet gluten content and sedimentation under research region was significant within the 7.16 km variation range, and the anisotropic structure varies more evidently in longitudinal and latitudinal directions. The spatial raster maps could show the distribution and the variation trend of regional grain quality effectively. 【Conclusion】 The result indicated that the IC based simulation on regional spatial variation of wheat grain quality is feasible. This study might provide reference for analysis of ecological variation on crop quality.

    Effects of Nitrogen Top-Dressing at Jointing Stage on Pasting Properties of Spring and Autumn Sown Waxy Maize Flour #br#
    LU Da-lei,JING Li-quan,WANG De-cheng,HAN qing,GUO Huan-fen,ZHAO Jiu-ran,LU Wei-ping
    Scientia Agricultura Sinica. 2009, 42(9):  3096-3103 .  doi:10.3864/j.issn.0578-1752.2009.09.010
    Abstract ( 978 )   PDF (327KB) ( 732 )   Save
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    【Objective】 The aim of the study is to understand the effects of nitrogen top-dressing amount at jointing stage on flour pasting properties of spring and autumn sown waxy maize varieties. 【Method】 Research was carried out at the Experiment Farm of Yangzhou University in 2008. Three varieties (Suyunuo 5, Kennian 1 and Suyunuo 1, the controls of Chinese waxy maize in regional test) were used in the study. The effects of nitrogen top-dressing amount (0, 150 and 300 kg?hm-2) at jointing stage on flour pasting properties of spring and autumn sown waxy maize were clarified by Rapid Visco Analyzer (RVA). 【Result】 Waxy maize flour pasting properties were significantly affected by the single factor and interactions of nitrogen top-dressing amount at jointing stage, sown dates and varieties. In general, the flour present higher peak viscosity, breakdown, trough viscosity and final viscosity, shorter peak time and lower pasting temperature when nitrogen top-dressing amount was 150 kg?hm-2 and in autumn-sown treatment, and those RVA characteristics were better for Suyunuo 5. Significantly positive correlations were observed among peak viscosity, trough viscosity, breakdown and final viscosity, and those four parameters were negatively correlated to peak time and pasting temperature. In addition, peak time was observed positively correlated to pasting temperature, and setback was positively correlated to final viscosity. 【Conclusion】Considering the pasting properties in different treatments, the autumn sown waxy maize varieties and nitrogen top-dressing amount at jointing stage was suitable (150 kg?hm-2 in this experiment) and could got the flour with higher peak viscosity and breakdown, shorter peak time and lower pasting temperature.

    PLANT PROTECTION
    Cloning and Chromosome Mapping of a Germin-Like Protein Gene in Wheat and Its Expression in Response to Infection with Wheat Powdery Mildew#br#
    WANG Jun-mei,SUN Yan-fei,LIU Hong-yan,KANG Zhen-sheng,XU Hong-ming
    Scientia Agricultura Sinica. 2009, 42(9):  3104-3111 .  doi:10.3864/j.issn.0578-1752.2009.09.011
    Abstract ( 894 )   PDF (394KB) ( 1119 )   Save
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    【Objective】 The aim of this study is to clone a wheat germin-like protein gene induced by Bgt (Blumeria graminis f. sp. tritici), analyse the expression pattern in susceptible and resistant plants and elucidate the molecular mechanism of the gene in resistance to powdery mildew. 【Method】 Gene chip hybridization, in silico cloning and RT-PCR methods were used to clone a wheat germin-like gene induced by Bgt, named as TaGLP5 (GenBank accession number FJ594470). A set of nulli-tetrasomic lines of Chinese Spring were employed to locate the gene. Its expression pattern at transcription level was investigated by quantitative PCR in resistant and susceptible progenies. 【Result】 A wheat germin-like protein, TaGLP5 with a complete ORF was cloned. Phylogenetic assay indicated that this gene differed from the known germin-like proteins from gramineous family. It was located on chromosome 5A. Quantitative PCR showed that the gene was induced by Bgt and higher expression in resistant plants than in susceptible ones before 24 h of inoculation. These will provide an important reference for further study on the gene. 【Conclusion】 A new wheat germin-like protein was obtained in this study. The expression patterns results revealed that TaGLP5 was up-regulated in both resistant and susceptible plants. However, the expression in quantity and time was different. It was speculated that TaGLP5 was involved in wheat defense response to Bgt.

    Development of Genomic SSR Markers for Phytophthora sojae#br#
    XU Jing-jing,WANG Xiao-ming,DUAN Can-xing,WU Xiao-fei,ZHU Zhen-dong
    Scientia Agricultura Sinica. 2009, 42(9):  3112-3122 .  doi:10.3864/j.issn.0578-1752.2009.09.012
    Abstract ( 927 )   PDF (427KB) ( 842 )   Save
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    【Objective】 This study was aimed at developing genomic SSR markers for Phytophthora sojae and offering ideal molecular markers for studying it and relative species in depth on molecular level. 【Method】 Using Fast PCR software to screening SSRs in complete genome sequence of P. sojae, suitable sequence regions harboring SSR were selected for design of markers using Primer5.0 software, and synthesized primers were used to amplify genomic DNA of selected isolates of P. sojae and other Phytophthora species. 【Result】 Of the designed 260 primer pairs, 213 (81.9%) could amplify characteristic SSR fragments against 10 different isolates of P. sojae, and 114 (53.5%) primer pairs amplified polymorphic fragments among the 10 isolates. The results for transferability test showed that 14.6%-28.6% primer pairs could effectively amplify in 8 selected Phytophthora species, respectively. Clustering Phytophthora spp and P. sojae based on 10 SSR markers data, these markers could clearly differentiate selected Phytophthora species. 【Conclusion】 The genomic SSR markers of P. sojae are highly polymorphic, and should be the ideal tools for studies of genetic variation, genetic diversity, and development of genetic linkage maps of the pathogen. Some SSR primers have transferability in other Phytophthora species, and could be used in phylogenetic analysis, species identification and differentiation and SSR marker development of Phytophthora. Especially, these genomic SSR markers have been located in P. sojae genome, thus facilitating lagging and cloning the functional genes of P. sojae, and carrying comparative genomics study of Phytophthora.

    Gene Construction, Expression and Biological Activity Analyses of Disulfide Stabilized Fv Fragment Against Xac#br#
    WANG Zhong-kang,LI Pan-zhi,YUAN Qing,YU Hong,LI Meng,YIN You-ping
    Scientia Agricultura Sinica. 2009, 42(9):  3123-3130 .  doi:10.3864/j.issn.0578-1752.2009.09.013
    Abstract ( 949 )   PDF (552KB) ( 837 )   Save
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    【Objective】To construct the gene of mouse anti-Xac disulfide stabilized Fv fragments (dsFv), express and refold to the form of dsFv with biological activity.【Method】The genes of VH and VL raised against Xac were mutated by the method of PCR-based mutagenesis, and then cloned into expression plasmid. The recombinant plasmids were transformed into E. coli BL21 (DE3) strain and these two genes were expressed. The inclusion body proteins of VH and VL were harvested and dissolved, and then diluted into refolding solution pro rata to form dsFv antibody which was further purified by HisTrap HP column. The products of expression and renaturation were analyzed by SDS-PAGE and western-blot. The affinity of dsFv to Xac-LPS was determined by BIAcore. The specificity and stability were detected by ELISA.【Result】 Sequence analysis proved that cysteines were introduced into VH44 and VL100, and the genes expressed in E.coli BL21 (DE3). Most of the protein existed in the form of inclusion body, the expression products were refolded successfully. SDS-PAGE showed that the molecular masses were 23 kD for VH and VL, and 46 kD for dsFv. BIAcore analysis showed that dsFv retained high affinity to Xac-LPS with an affinity constant (KD) of 3.40×10-10M. ELISA indicated that dsFv had a high specificity to Xac. Compared with scFv’s, the thermal stability of dsFv elevated nearly 20℃.【Conclusion】dsFv was expressed and refolded successfully, which maintained high affinity, specificity and stability. This research established a solid basis for rapid diagnosis of Xac.

    A Preliminary Investigation of a Mutant Melon Plant edr2 on Resistance to Gummy Stem Blight#br#
    REN Hai-ying,FANG Li,RU Shui-jiang,WANG Han-rong
    Scientia Agricultura Sinica. 2009, 42(9):  3131-3138 .  doi:10.3864/j.issn.0578-1752.2009.09.014
    Abstract ( 1062 )   PDF (487KB) ( 851 )   Save
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    【Objective】 Gummy stem blight is a prevalence destructive disease of melon. To identify the genes involved in the disease resistance to Didymella bryoniae is the crucial step for breeding resistant melon. 【Methods】 Agrobacterium mediated T-DNA insertion method was used to generate transgenic mellon plants from tissue of cotyledon. The levels of disease resistance of the melon plants were measured and detemined by inoculation of Didymella bryoniae in vitro and in vivo. 【Results】 A melon plant with enhanced resistance to gummy stem blight, named edr2, was identified from 2000 transgenic plants obtained. The mutant has one copy of T-DNA inserted in its genome, and the co-segregation was found between the resistance to gummy stem blight and resistance to kanamycin gene nptII. The germination rate of the spores and growth of germ tubes and hypha were slower in edr2 mutant plant than in wild type plant when Didymella bryoniae was inoculated. Programmed cell death was observed on the leaves of edr2 mutant plant leaves, but not on the ones of wild type melon plant. 【Conclusion】 The edr2 mutant melon plant obtained from T-DNA insertion demonstrated a higher level of disease resistance. The results provide useful information for breeding new melon varieties for disease resistance, and understanding of the functions of the genes involving interaction of melon- Didymella bryoniae.

    Establishment of ISSR Reaction System of Fusarium and Its Analysis of Genetic Diversity #br#
    LI Rui-qian,HE Rui,ZHANG Yue-bing,XU Yu-mei,WANG Jian-ming
    Scientia Agricultura Sinica. 2009, 42(9):  3139-3146 .  doi:10.3864/j.issn.0578-1752.2009.09.015
    Abstract ( 1083 )   PDF (487KB) ( 1116 )   Save
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    【Objective】 To establish the ISSR reaction system of Fusarium and analyze the genetic diversity of its isolates. 【Method】 Utilization of ISSR-PCR enlargement reaction technique, the PCR ingredients was optimized and the ISSR premier was chosen. Analysis of the genetic diversity of Fusarium isolates through the established cluster map. 【Result】 The optimum conditions for ISSR-PCR were 2.0 mmol?L-1 Mg2+, 0.5 U Taq DNA polymerase, 0.2 mmol?L-1 dNTPs, 0.4 μmol?L-1 primers, 30 ng templates DNA in 20 μl reaction system and UBC885 as premier, the annealing temperature was 52℃. ISSR technique was used to analyze the diversity of 27 Fusarium isolates with 11 primers, and the results showed that 79 fragments were amplified; polymorphic loci were 65 which accounted for 82.3% in the total amplified fragments. The genetic diversity was analyzed according to the amplified results and a molecular dendrogram was constructed and the genetic similarities among Fusarium isolates were analyzed. The similarity coefficient of 27 Fusarium isolates was at 0.672-0.950 and at the level of 0.670 they were divided into 3 groups and 2 subgroups. 【Conclusion】 In this paper, the author has established the befitting ISSR-PCR reaction system for Fusarium and found there are manifest interspecific and intraspecific genetic diversity of Fusarium isolates. This technique can be used to analyze the interspecific and intraspecific genetic diversity of Fusarium in the future study.

    Distribution and Developmental Process of Heterodera glycines in Soybean Root#br#
    WANG Zhen-hua,SHI Li-bo,WU Hai-yan,LIU Jing,LI Xiu-xia
    Scientia Agricultura Sinica. 2009, 42(9):  3147-3153 .  doi:10.3864/j.issn.0578-1752.2009.09.016
    Abstract ( 1084 )   PDF (279KB) ( 828 )   Save
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    【Objective】 Dynamic distribution and developmental process of race 4 of Heterodera glycines (soybean cyst nematode, SCN) in the soybean root were monitored under field conditions in Shandong province during 2007 and 2008 in order to determine the developmental process and distribution of nematodes in soybean root, and provide information for effectively controlling the disease. 【Method】 The root systems were collected with mesh bag method and nematodes in root were stained by the method of fuchsin acid–NaCIO. The nematodes (including different stage juveniles) were counted under stereo microscope. 【Result】 The results of two years indicated that Np (nematodes per cm root) showed the similar sigmoid curve during 37 days of seedling age. The dynamic of development of the second-stage juveniles (J2), the third-stage juveniles (J3) and the fourth-stage juveniles (J4) were similar during the period of the experiment. The highest value of nematode density appeared at 4-5 weeks after soybean seedling emergence. All stage juveniles were distributed at different soil layers but mainly at 5-15 cm soil layer and J2 was the dominant population in each soil layer. 【Conclusion】 Under the average soil temperature around 20-22.2℃ in Shandong province, the first generation of SCN was finished at 22-35 days after soybean seedling emergence and the mature female was found at 22 days. From the results, the chemicals were suggested to be applied on the soil depths of 5-15 cm and lasted for 1 month after soybean seedling emergence and therefore to obtain the effective control of the nematodes..

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Spectral Characteristics and Estimation of Organic Matter Contents of Different Soil Types#br#
    ZHANG Juan-juan,TIAN Yong-chao,ZHU Yan,YAO Xia,CAO Wei-xing
    Scientia Agricultura Sinica. 2009, 42(9):  3154-3163 .  doi:10.3864/j.issn.0578-1752.2009.09.017
    Abstract ( 1033 )   PDF (585KB) ( 1252 )   Save
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    【Objective】 The objectives of the present study were to determine the key spectral parameters and models for estimating SOM content. 【Method】 The dried sample of five different soil types in China were analyzed for SOM content and hyperspectral reflectance within 350-2 500 nm, quantitative models of SOM using spectral index and BP neural network were established, respectively. 【Result】The results showed that correlation between spectral indices which composed of first derivative and SOM content were obviously stronger than those composed of original reflectance, especially derivative with Norris smoothing filter. The correlation sequence of SOM to different index types was DI>RI>ND which composed of spectral reflectance or the first derivative spectra. DI composed of first derivative of 554 nm and 1 398 nm gave a better prediction performance, with equation as y=184.2×exp[-1297×DI(D554, D1398)], coefficient of determination was 0.90. Testing of the monitoring models with independent data from different soil types indicated that R2, RMSE and RPD of validation were 0.84, 3.64 and 2.98, respectively. In addition, the scores computed by PLS were applied as input of BP neural network developed with over 99.56% of cumulative proportion of correlation matrix. R2 of calibration model was 0.98, and R2, RMSE, RPD of validation were 0.96, 2.24 and 4.83, respectively. Compared with BP neural network model, DI(D554, D1398) had a little lower prediction precision, but it could meet need of estimating of SOM content. 【Conclusion】 It is concluded that both of methods based on DI(D554, D1398) and BP neural network can estimate SOM content accurately.

    Effects of Different Fertilization Proportions on Organic Carbon Content of Black Soil and Carbon Pool Management Index#br#
    ZHAO Hong,Lü Yi-zhong,YANG Xi,ZHOU Lian-ren,JIN Xiang-sheng
    Scientia Agricultura Sinica. 2009, 42(9):  3164-3169 .  doi:10.3864/j.issn.0578-1752.2009.09.018
    Abstract ( 909 )   PDF (294KB) ( 945 )   Save
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    【Objective】 Effect of reducing chemical nitrogen fertilizer and increasing organic manure on soil organic carbon content and carbon pool management index (CPMI) were examined, and the best design of organic manure and chemical fertilization proportion was researched. 【Method】 Take typical black soil from Shuangcheng in Heilongjiang as an object, the five treatments: only chemical fertilizer, application of 5/6(N) +1/6 organic manure, application of 4/6(N)+2/6 organic manure, application of 3/6(N) +3/6 organic manure, application of 2/6(N) +4/6 organic manure were designed, choosing classical method for measuring organic carbon and active organic carbon content, and carbon pool management indexes were measured. 【Result】 The organic carbon content and active organic carbon content in the treatment of application 2/6(N) +4/6 organic manure and the treatment of application of 3/6(N) +3/6 organic manure was higher than the treatment of application of only chemical fertilizer by 15.7%, 6.4% and 8.2%, 10.9% respectively ; application of 3/6(N) +3/6 organic manure and application 5/6(N) +1/6 organic manure had the highest CPMI value, and the average value was both both higher than application of 2/6(N) +4/6 organic manure, application of only chemical fertilizer by 13.0%; the average grain yield of application only chemical fertilizer, application 5/6(N) +1/6 organic manure, application of 3/6(N) +3/6 organic manure was higher than the other treatment by 9.1%. 【Conclusion】 The different proportions of organic manure and chemical fertilizer has a significant influence on organic carbon, active organic carbon, CPMI and grain yield, application of 3/6(N) +3/6 organic manure can increase organic carbon, active organic carbon, CPMI and grain yield significantly, so it is the best proportion of organic manure and chemical fertilizer in Shuangcheng.

    Analysis of the Nitrogen Transfer, Nitrogen Uptake and Utilization in the Two Relay-Planting Systems #br#
    YONG Tai-wen,YANG Wen-yu,REN Wan-jun,FAN Gao-qiong,XIANG Da-bing
    Scientia Agricultura Sinica. 2009, 42(9):  3170-3178 .  doi:10.3864/j.issn.0578-1752.2009.09.019
    Abstract ( 925 )   PDF (250KB) ( 1080 )   Save
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    【Objective】 The aim of this paper was to study the mechanisms of interspecific nitrogen facilitation and transfer in the relay-planting systems of wheat/maize/soybean and wheat/maize/sweet potato. 【Method】 The methods of root barrier and 15N-isotope dilution were used to investigate the nitrogen transfer, nitrogen uptake and utilization in the two relay-planting systems.【Result】 Comparing the no barrier with solid barrier, the results showed that the 15N total uptake and crop recovery rate of wheat were highly remarkable and 15N% abundance and N% content of soil was lower obviously than that of solid barrier. In the wheat/maize/soybean system with no barrier, the 15N total uptake, 15N grain uptake, 15N crop recovery rate, soil 15N% abundance and N% content of maize increased by 17.62%, 24.52%, 17.63%, 13.9% and 10.1%, respectively. But in the wheat/maize/sweetpotato system with no barrier, the value of above index reduced by 50.19%, 42.58%, 33.42%, 29.6% and 5.2%, respectively. For soybean, the 15N total uptake, 15N grain uptake, and 15N crop recovery rate reduced, but the soil N% content increased by 6.06%. For sweetpotato, the 15N total uptake and crop recovery rate increased, but soil 15N% abundance and N% content reduced by 0.9% and 4.95%. 【Conclusion】 There existed nitrogen interspecific competition and facilitation and nitrogen transfer in the two relay-planting systems. In the wheat/maize/soybean system, the nitrogen uptake from fertilizer, soil fertility remaining and sustainable crop production were better than the system of wheat/maize/sweetpotato.

    Field Experiments on Evaporation and Evapotranspiration of Winter Wheat Under Sprinkler Irrigation#br#
    YU Li-peng,HUANG Guan-hua,LIU Hai-jun,WANG Xiang-ping,WANG Ming-qiang
    Scientia Agricultura Sinica. 2009, 42(9):  3179-3186 .  doi:10.3864/j.issn.0578-1752.2009.09.020
    Abstract ( 881 )   PDF (459KB) ( 1012 )   Save
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    【Objective】 The objective of the experiment was to evaluate the evapotranspiration (ET) of winter wheat under sprinkler irrigation in Beijing area, which is useful to the field sprinkler irrigation management practices of winter wheat. 【Method】Field experiments were conducted in 2005-2008 growing seasons in an experimental station with different irrigation depths. 【Result】 Results indicated that variation of soil water content occurred within 0-40 cm soil layer. The seasonal ET of winter wheat generally increased with the increasing of irrigation amount, while the seasonal usage of soil water had a negative relationship with irrigation amount. Soil evaporation was about 25% of winter wheat ET during the period from reviving to maturity. Soil evaporation increased while the proportion of soil evaporation to ET decreased as the increase of irrigation amount. 【Conclusion】 Sprinkler irrigation scheduling with a relatively large irrigation quota and low irrigation frequency can reduce soil evaporation and promote irrigation water use efficiency.

    Advance in Research of Supplemental Irrigation of Collected Rain Water for Eco- Agriculture in Semi-Arid Loess Plateau of China#br#
    ZHAO Xi-ning,WU Pu-te,FENG Hao,WANG You-ke
    Scientia Agricultura Sinica. 2009, 42(9):  3187-3194 .  doi:10.3864/j.issn.0578-1752.2009.09.021
    Abstract ( 1129 )   PDF (250KB) ( 873 )   Save
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    The semi-arid Loess Plateau is a central zone of Chinese rainfed farming. Drought and soil erosion are the two major obstacles to economic development in the semi-arid Loess Plateau. The conservation agriculture and runoff agriculture has been developed for several decades here. However, its regulative role in rain water was very limited, and the problems of agriculture production are still going on, such as severe seasonal drought and water shortage, low agriculture productivity, fragile ecological environment, low yield-invest ratio. Supplemental irrigation of collected rain water is an important form of rainfall regulation and utilization on the basis of sophisticated technologies and theories of conservation agriculture and runoff agriculture, which not only come to fulfillment rainfall collecting in spatial location like the conservation agriculture and runoff agriculture measure, but also come to true rainfall collecting in time distribution by principle of superposition. Agricultural productivity will be highly increased by using rain water collecting for supplemental irrigation. It not only brings about the integration of ecological reconstruction with economic development, but also provides a new approach to the sustainable development of agriculture and removing two biggest stumbling blocks, drought and soil erosion. It is suggested that this technology will become a strategic measure and comprehensive development model in semi-arid Loess Plateau of China.

    HORTICULTURE
    Obtaining and Utilization of DH Lines in Pakchoi (Brassica rapa L. ssp. chinensis L.)#br#
    FENG Hui,YANG Shuo,WANG Chao-nan,FU Ying
    Scientia Agricultura Sinica. 2009, 42(9):  3195-3202 .  doi:10.3864/j.issn.0578-1752.2009.09.022
    Abstract ( 868 )   PDF (418KB) ( 823 )   Save
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    【Objective】 The study is to develop diversified DH lines in pakchoi for the practice of heterosis breeding. 【Method】 Isolated microspore culture was employed in 15 hybrid varieties. An efficient culture system was established through the optimization of culture conditions. 【Result】 Totally 373 regenerated plants were obtained, in which 251 were identified as double haploid. Furthermore, seven excellent DH lines were screened out. Two improved F1 combinations were made based on such DH lines. 【Conclusion】 A technical system was successfully established for heterosis breeding of pakchoi with DH lines as parents created by isolated microspore culture.

    Response of Yield and Quality of Hot Pepper in Greenhouse to Irrigation Control at Different Stages in Arid Northwest China #br#
    CHEN Ping,DU Tai-sheng,WANG Feng,DONG Ping-guo
    Scientia Agricultura Sinica. 2009, 42(9):  3203-3208 .  doi:10.3864/j.issn.0578-1752.2009.09.023
    Abstract ( 851 )   PDF (297KB) ( 807 )   Save
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    【Objective】 The objective of this study is to bring forward a suitable water-saving efficient irrigation pattern for improving fruit quality based on the response of quality of greenhouse crops to water stress. 【Method】 An experiment was conducted to evaluate the response of yield, fruit quality and water use efficiency to different water stress treatments at seedling, flowering-fruit setting and fruit maturing stages of hot pepper (Capsicum annum L. cv. Lonjiao 1, a local hybrid variety) in greenhouse. 【Result】 The results showed that fruit maturing stage was the most sensitive stage to the yield thus severe water stress should not be applied at this stages. The soluble protein and dry mass of the fruit were not sensitive, but vitamin C, total soluble solid content and hardness of the fruit were sensitive to water stress at flowering-fruit setting and fruit maturing stages. The mathematical expression that better fits the relation between the yield, fruit quality and evapotranspiration is logarithm function. 【Conclusion】 It suggested that soil water content should be controlled between 45% and 60% of field water capacity (θf) in depth of 60 cm at flowering-fruit setting stage and 80% to 90% θf at seedling and fruit maturing stages is a suitable water-saving efficient irrigation pattern for improving fruit quality, which can improve fruit vitamin C, total soluble solid content and hardness with higher yield but less water.

    Effect of Vineyard Cover Crop on Main Monomeric Phenols of Grape Berry and Wine in Vitis viniferal L. cv. Cabernet Sauvignon#br#
    XI Zhu-mei,ZHANG Zhen-wen,CHENG Yu-feng,CHENG Zhe,LI Hua
    Scientia Agricultura Sinica. 2009, 42(9):  3209-3215 .  doi:10.3864/j.issn.0578-1752.2009.09.024
    Abstract ( 990 )   PDF (309KB) ( 847 )   Save
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    【Objective】 This study was conducted to determine the effect of cover crop inter-row in vineyard on main mono-phenol content of grape berry and wine. 【Method】 Three such cover crops, two perennial legumes (white clover and alfalfa) and a perennial gramineous grass (tall fescue) were sown in vineyard. The main phenolic compounds of mature grape berry and wines vinified under the same conditions were extracted with ethyl acetate and trichloromethane and analyzed by high-performance liquid chromatography (HPLC) and compared with that in soil tillage. 【Result】 A total of 10 phenolic compounds were identified and quantified in the different grape berry and wines, including nonflavonoids (hydroxybenzoic and hydroxycinnamic acids) and flavonoids (flavanols and flavonols). The concentration of flavonoid compounds (409.43 to 538.63 mg?kg-1 and 56.16 to 81.30 mg?L-1) was higher than nonflavonoids (76.91 to 98.85 mg?kg-1 and 30.65 to 41.22 mg?L-1) for Cabernet Sauvignon grape and wine in different treatments. In the flavonoid phenolics, catechin was the most abundant in different grapes and wines, accounting for 74.94% to 79.70% and 48.60% to 50.62% of total nonanthocyanin phenolics quantified, respectively. Compared to soil tillage, the sward treatments showed a higher content of main mono-phenol and total nonanthocyanin phenolics in grapes and wines. There were significant differences between two cover crop treatments (tall fescue, white clover) and soil tillage in the content of benzoic acid, salicylic acid, caffeic acid, catechin and total phenolics in grape berry (P<0.05 or P<0.01). The wine from tall fescue cover crop had the highest gallic acid, caffeic acid and catechin. Cover crop system increased the total nonanthocyanin phenolics of grapes and wines in order of the four treatments: tall fescue, white clover, alfalfa and soil tillage (control). 【Conclusion】 Cover crop in vineyard increased total phenols of grape berry and wine, thus improved the quality of wine evidently.

    Changes of Aroma Components of Different Developmental Fruits of High-Bush Blueberry #br#
    ZHANG Chun-yu,LI Ya-dong,ZHANG Zhi-dong,LIU Hai-guang,WU Lin,WANG Jing-ying
    Scientia Agricultura Sinica. 2009, 42(9):  3216-3223 .  doi:10.3864/j.issn.0578-1752.2009.09.025
    Abstract ( 1215 )   PDF (456KB) ( 997 )   Save
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    【Objective】 Changes of aroma components of high-bush blueberry fruits at different development stages were studied. The results could provide the basic information for further discussing synthesis mechanism of high-bush blueberry aroma components, and provide the theoretical evidence for regulation of high-bush blueberry quality. 【Method】 The aroma components of Duke and Bluecrop cultivars at three different developmental stages including green stage, pink stage, and blue stage in high-bush blueberry were analyzed by static headspace and gas chromatography-mass spectrometry (GC-MS). 【Result】 The results showed that 48 kinds of volatile components were detected at three different developmental stages of high-bush blueberry fruit and the main volatile compounds were alcohols, esters, and terpenes, in which the formation of esters were started at pink stage. For Duke, the rapid formation stage of esters was blue stage. For Bluecrop, the rapid formation stage of alcohols and esters was blue stage and pink stage, respectively. The content of terpenes increased significantly during fruit maturation, in which the terpenes content of Duke was obvious higher than that of Bluecrop at every developmental stage. The character impact components (CICs) were formed at pink stage of both Duck and Bluecrop. The CICs of Duke were ethyl butanoate, damascenone, ethyl 2-methylbutyrate, β-linalool, D-limonene, and 2-butanone. The CICs of Bluecrop were ethyl 2-methylbutyrate, (E)-3-hexenyl acetate, β-linalool, ethyl butanoate, hexyl acetate, (E)-2-hexenal, and (Z)-3-hexen-1-ol, and D-limonene. The esters and terpenes were the most main CICs of high-bush blueberry during fruit maturation. 【Conclusion】 The aroma components of high-bush blueberry fruit were formed mostly at pink and blue stages, so it is important to regulate these two stages in order to improve the quality of high-bush blueberry.

    Identification of Pathogens Causing Apple Fruit Bitter Rot in Shaanxi and He’nan Provinces#br#
    ZHANG Rong,WANG Su-fang,CUI Jing-qiu,SUN Guang-yu
    Scientia Agricultura Sinica. 2009, 42(9):  3224-3229 .  doi:10.3864/j.issn.0578-1752.2009.09.026
    Abstract ( 1004 )   PDF (491KB) ( 1045 )   Save
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    【Objective】 Determining the possible pathogens of apple fruit bitter rot in Shaanxi and He’nan provinces of China. 【Method】 The pathogens were isolated and their pathogenicities were determined. Identification was carried out by using traditional morphological methods and sequencing of rDNA-ITS with universal primers and species-specific primer CaInt2. 【Result】 One hundred and ninety two samples of diseased apple fruits were collected from eleven locations in Shaanxi and He’nan provinces of China. Two fungal species, Colletotrichum gloeosporioides (Penz.) Sacc. and C. acutatum J.H. Simmonds were found as possible pathogens of apple fruit bitter rot in these regions. The two fungal pathogens can be distinguished on the basis of conidial morphology, colony color, growth rate and specific amplification by primers CaInt2/ITS4. 【Conclusion】 It has been confirmed that the apple fruit bitter rot in China is caused by two fungal species, Colletotrichum gloeosporioides and C. acutatum. Both species were distributed in the districts sampled, but C. gloeosporioides was more often isolated.

    Induction of Phenolic Compounds and Lignin in Cultured Tobacco Cells Treated with Riboflavin#br#
    LIU Fei,LIU Hui,LIANG Yuan-cun,LIU Ai-xin,WEI Fang-fang,LIN Jin
    Scientia Agricultura Sinica. 2009, 42(9):  3230-3235 .  doi:10.3864/j.issn.0578-1752.2009.09.027
    Abstract ( 1156 )   PDF (369KB) ( 774 )   Save
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    【Objective】 To investigate the effects of riboflavin on phenylpropanoid metabolism in tobacco cells. The activity of phenylalanine ammonia-lyase (PAL) and peroxidase (POD), accumulation of phenolic compounds and lignin were determined. 【Method】 Tobacco suspension cells treated with 1 mmol?L-1 riboflavin were used to investigate the effect of the phenylpropanoid metabolism by biochemical, cytochemical and HPLC (high performance liquid chromatography) analysis. 【Result】 The activity of PAL and POD increased and reached the highest level at 12 h and 24 h after treatment with riboflavin, respectively. The observation of bright blue fluorescence indicated that more phenolic compounds accumulated in the cells under UV light, extracts from tobacco cells and their culture filtrate showed strong accumulation of scopoletin within 48 h after treatment, and reached a peak at 12 h after treatment. Lignin deposition strongly increased by 1.5 and 1.8 times respectively at 24 and 48 h after elicitation when compared with the control. 【Conclusion】 These results suggest that the increase in related enzymes, phenolic compounds and lignin was induced by riboflavin in tobacco suspension cells.

    Self-Compatibility of 22 Rosa rugosa Thunb. Resources in China#br#
    YU Xiao-yan,ZHAO Lan-yong,FENG Zhen,QI Hai-ying,XU Zong-da,ZHU Xiu-qin
    Scientia Agricultura Sinica. 2009, 42(9):  3236-3242 .  doi:10.3864/j.issn.0578-1752.2009.09.028
    Abstract ( 1219 )   PDF (331KB) ( 825 )   Save
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    【Objective】 The aim of this experiment is to reveal the self-compatibility of Rosa rugosa Thunb. It is meaningful to improve the theory and techniques of breeding ornamental plant in Rosa especially when Rosa rugosa Thunb. used as maternal parent. 【Method】Analysis of the rate of fertile fruits and average seed number combined with fluorescent microscope observation of pollen tubes was employed in this study. 【Result】 No fruit was produced and the pollen tubes were arrested at the upper one third of the styles when all the plant materials were self-pollinated on anthesis and 4 days before anthesis. The self-compatibility of Rosa rugosa Thunb. could be improved when they were pollinated before anthesis, and the effect of self-pollination on 2 days before anthesis was the best. For the rate of fertile fruit and average seed number of Rosa rugosa Thunb., significant and positive relationship was found between self-pollination in bud period and open pollination. 【Conclusion】 Rosa rugosa Thunb. is completely gametophytic self-incompatible. The self-compatibility of Rosa rugosa Thunb. can be improved by pollination in bud period, but the effect varies with the change of pollination periods and cultivars.

    STORAGE·FRESH-KEEPING·PROCESSING
    Construction of Traceability System for Quality Safety of Cereal and Oil Products#br#
    ZHENG Huo-guo,LIU Shi-hong,MENG Hong,HU Hai-yan,SU Xiao-lu
    Scientia Agricultura Sinica. 2009, 42(9):  3243-3249 .  doi:10.3864/j.issn.0578-1752.2009.09.029
    Abstract ( 979 )   PDF (404KB) ( 1331 )   Save
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    【Objective】 To trace the quality safety information of cereal and oil products from "farm to table". 【Method】The cereal and oil products quality safety traceability system, which is multi-level and multi-role one, was designed and implemented with information encode, multi-platform tracing and hardware R&D technology, according to the study on cereal and oil products. 【Result】 The function of the system includes basic information management, standards management, production management, FMECA module and tracing management. The system not only provides the management in origin, processing, circulation and consumption of cereal and oil products for enterprises, but also provides tracing service for customers and supervisors by telephone, internet, SMS, touch machine and mobile traceability terminal. 【Conclusion】 This study has provided an effective way for cereal and oil products quality safety management, meanwhile , set an example for other agricultural products.

    Effect of Nano-Packaging Material on Quality of Flammulina velutipes#br#
    YANG Yan-ting,YANG Qin,YANG Fang-mei,XIN Zhi-hong,ZHAO Li-yan,YU Zhi-fang,HU Qiu-hui
    Scientia Agricultura Sinica. 2009, 42(9):  3250-3258 .  doi:10.3864/j.issn.0578-1752.2009.09.030
    Abstract ( 1191 )   PDF (611KB) ( 1347 )   Save
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    【Objective】 A nano-packaging material was prepared by blending polyethylene (PE) with nano Ag, TiO2, attapulgite and it’s effects on the preservation quality of Flammulina velutipes were investigated. 【Method】 The morphological and physical properties of nanocomposite were tested via scanning electron microscopy and barrier properties test. During the 15-day storage at 4℃, the sensory quality, weight loss, decay rates, browning degree, relative conductivity, soluble solids content, soluble protein and free amino acid parameters of Flammulina velutipes treated with nano-packaging material and regular PE packaging were evaluated. 【Result】 The results indicated that homogeneous distribution nanoparticles contributed to better physical properties of nano-packaging materials. The signi?cant differences were exhibited between the packages with and without nanoparticles on the sensory quality, physiological indexes and main nutritional quality of Flammulina velutipes. Weight loss, rot index, relative conductivity and browning degree of Flammulina velutipes in nano-packaging material were decreased by 43.14%, 97.13%, 29.77% and 12.68% compared to regular packaging respectively. Additionally, the soluble solids content, soluble protein and free amino acid content in the Flammulina velutipes under nano-packaging material were also well presented. 【Conclusion】 Nano-packaging material greatly improved the preservation quality of Flammulina velutipes compared with regular packaging.

    Effects of Irradiation on Biochemical Properties and Antigenicity of Crab Allergen#br#
    ZHANG Ming-qi,GAO Mei-xu,ZHI Yu-xiang,PAN Jia-rong,LI Shu-rong,Lü Shu-xia
    Scientia Agricultura Sinica. 2009, 42(9):  3259-3264 .  doi:10.3864/j.issn.0578-1752.2009.09.031
    Abstract ( 964 )   PDF (381KB) ( 990 )   Save
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    【Objective】 The study was conducted to evaluate the effect of γ-irradiation on the biochemical and immunological properties of an crab allergen. 【Method】 Crab protein solutions containing allergen were irradiated at 3, 5, 7 and 9 kGy. The changes of concentration, turbidity and hydrophobicity of the irradiated protein solution were determined by UV and fluorescence spectrophotometer. SDS-PAGE electrophoresis was used to analyze the change of molecular weight. Changes in antigenicity of the irradiated protein were observed by competitive indirect enzyme-linked immunosorbent assay (ELISA) and Western blotting. 【Result】 The turbidity and hydrophobicity of the crab allergen solution increased and its concentration decreased with the increase of irradiation dose. Patterns detected by the SDS-PAGE and the immunoblot showed that the band of allergy protein disappeared. Inhibition abilities of allergy protein against polyclonal IgG decreased with the increasing irradiation dose. 【Conclusion】 The conformation and epitopes on crab allergen were structurally altered by irradiation. So γ-irradiation may provide a potential method for cowering the antigenicity of crab allergen.

    ANIMAL SCIENCE·RESOURCE INSECT
    Expression Profiling of Intestinal N-Acetylglutamate Synthase in Pre-Weaned Tibetan Suckling Piglets #br#
    SONG Xiao-yan,GENG Mei-mei,CHU Wu-ying,LI Tie-jun,WANG Wen-ce,GU Wan-ting,SHI Chang-you,HUANG Rui-lin,YIN Yu-long,WU Guo-yao
    Scientia Agricultura Sinica. 2009, 42(9):  3265-3271 .  doi:10.3864/j.issn.0578-1752.2009.09.032
    Abstract ( 977 )   PDF (676KB) ( 667 )   Save
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    【Objective】 This study was conducted to investigate the mechanism for the reduction of endogenous arginine synthesis by profiling the developmental expression pattern of intestinal N-acetylglutamate synthase (NAGS) in pre-weaned Tibetan piglets. 【Method】 The gene of NAGS was cloned from Tibetan piglets, and the rabbit antiserum against the purified NAGS was prepared. The gene expression level and protein content of NAGS were analyzed using real time PCR and western blotting methods in jejunum and ileum of Tibetan piglets at 1, 7, 21, 28 and 35 days of age, respectively. 【Result】 With the age increasing from day 1 to 35, both the mRNA expression level (P<0.05) and protein content (P<0.01) of NAGS were gradually decreased. 【Conclusion】 The expression levels of NAGS mRNA and protein were decreased during sucking period as the Tibetan piglet grew up. The decreased expression level of NAGS may be one of the important mechanisms for the reduction of endogenous arginine synthesis in pre-weaned suckling piglets.

    Correlation Analysis Between FATP1 Gene Polymorphism and Carcass Traits in Chicken#br#
    ZHANG Long,ZHU Fang-Jun,WANG Yan,LAN Xi,Lü Rong-Ping,LIU Yi-Ping,ZHU Qing
    Scientia Agricultura Sinica. 2009, 42(9):  3272-3278 .  doi:10.3864/j.issn.0578-1752.2009.09.033
    Abstract ( 955 )   PDF (403KB) ( 719 )   Save
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    【Objective】 The polymorphisms of FATP1 and their correlation to carcass traits in chicken were investigated for purpose of providing molecular maker information to facilitate the breeding efficiency. 【Method】 PCR-SSCP was applied to analyze the polymorphisms of partial exon loci in FATP1 gene in 381 Chinese chicken from Dahen breeding company. Genotype frequencies, allelic frequencies, correlation analysis between the polymorphisms and carcass traits were estimated. 【Result】 The result showed that 3 genotypes and 9 haplotypes combinations were found at the two loci. The genotypes of P3 loci had a significant effect on live weight, carcass weight, semi-eviscerated weight, eviscerated weight, breast weight and leg muscle weight. Different genotypes had a remarkable influence on leg muscle weight and abdominal fat percent in the P4 location. In addition, the frequencies of nine haplotypes combinations were 0.538, 0.052, 0.265, 0.029, 0.045, 0.037, 0.024, 0.005 and 0.005, and different haplotypes combinations affect the breast muscle weight, breast muscle percent and abdominal fat percent. 【Conclusion】 The correlation was detected between different genotypes and haplotypes combinations and partial carcass traits and abdominal fat percent at these two loci.

    Polymorphisms of κ–Casein Gene Exon4 and 5 and Its Association with Milk Performance Traits in Chinese Holstein Cattle#br#
    JU Zhi-hua,WANG Hong-mei,LI Qiu-ling,HUANG Jin-ming,LI Jian-bin,AN Li-guo,YANG Gui-wen,ZHONG Ji-feng,WANG Chang-fa
    Scientia Agricultura Sinica. 2009, 42(9):  3279-3287 .  doi:10.3864/j.issn.0578-1752.2009.09.034
    Abstract ( 951 )   PDF (718KB) ( 866 )   Save
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    【Objective】 The polymorphisms of κ–casein gene exon 4 and 5 and their correlation to milk performance traits in Chinese Holstein cattle were investigated for the purpose of providing molecular maker information to facilitate the breeding efficiency of high milk protein and milk fat. 【Method】 Sequencing, PCR-RFLP and CRS-PCR were applied to analyze the polymorphisms of six loci in κ–casein gene exon 4 and 5 in 544 Chinese Holstein cattle. 【Result】 Five SNPs in the exon five (A12907G, G12950A, C12989T, A13028G, T12980C) and one SNP in the exon four were found and A12907G, G12950A, C12989T, A13028G were closely linked. At locus A10996T, the cows with genotype TT and AT showed higher fat rate than those with genotypes AA (P<0.05). At locus T12980C, the cows with genotype TC and CC was very significantly (P<0.01) and significantly (P<0.05) higher than TT genotype; with genotype TC and CC showed higher protein rate than those with genotypes TT (P<0.05). At the remaining four loci, the cows with genotype DE showed higher fat rate than those with genotypes DD (P<0.01). Seven haplotype and 16 haplotype combinations were found in 398 Chinese Holstein cattle,the cows with haplotype combinations H6H6 had the highest milk fat percentage and secondly was haplotype combinations H1H4. The cows with haplotype combinations H1H4 had the highest milk protein percentage and with haplotype combinations H5H6 had the lowest milk fat percentage and milk protein percentage. 【Conclusion】 Considering the distribution frequencies of haplotype combinations, κ-casein gene H1H4 is a favorable haplotype combinations to the milk fat percentage and milk protein percentage and can be chosen as the molecular markers of choosing high milk fat percentage and milk protein percentage.

    Relationship Between the mRNA Expression Level of TGF-β Receptors in Hu Sheep's Tissues and the Number of Ovulation#br#
    LI Er-lin,LI Qi-fa,LI Mei-li,XU Ye-fen,HU Dong-li,ZHU Hong-sheng,LI Xin-fu,XIE Xin-hua, CHEN Ling,XIE Zhuang
    Scientia Agricultura Sinica. 2009, 42(9):  3288-3295 .  doi:10.3864/j.issn.0578-1752.2009.09.035
    Abstract ( 910 )   PDF (581KB) ( 716 )   Save
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    【Objective】 The impact of TGF-β receptors (TGF-βRⅠ,TGF-βRⅡ) on the number of ovulation was studied by analyzing the tissue expression pattern and the relationship between the mRNA expression level of TGF-β receptors and the number of ovulation. 【Method】 Sixteen multiparous ewes were selected to investigate, eight which bred multi-lamb were used as the high-yield group, the other eight which bred single lamb were used as control group. Cloprostenol sodium were injected to make the synchronization of estrus treatment, then slaughtered within 48-60 h after empathema, two side ovaries were collected after slaughtering, the number of corpus rubrum on the ovaries was counted to deternine the number of ovulation; the tissues such as hypothalamus, hypophysis were collected from one ewe in the high-yielding group, to make a tissue expression analysis. The relationship between the mRNA expression level of TGF-β receptors and the number of ovulation, which were detected by real-time fluorescent quantitative PCR, were analyzed.【Result】 TGF-βRⅠ’s relative expression level in the reproductive organ was only significantly higher than in the lung and muscle (P<0.01). TGF-βRⅡ’s relative expression level in reproductive organ was significantly higher than that of other tissues (P<0.01), it is a high-expressed gene in ovary. TGF-βRⅠand TGF-βRⅡ’s mRNA expression level in the ovaries of the high-yield group were significantly higher (P<0.01) and obviously higher (P=0.011) than the control group, respectively. TGF-βRⅠand TGF-βRⅡ’s mRNA expression level have a positive correlation with the number of ovulation, the correlation coefficient were 0.562 (P>0.05) and 0.711 (P<0.05), respectively. 【Conclusion】 TGF-β receptors showed the high level in ovary, TGF-βRⅠand TGF-βRⅡ’s mRNA expression level in the ovaries of the high-yielding group were extremely or obviously higher than the control group, furthermore, it had a positive correlation or a significant positive correlation with the number of ovulation, so it illusted that TGF-β receptors have a close relationship with high-yielding Hu sheep.

    VETERINARY SCIENCE
    Effect of Co- infection with REV and ALV-J on T Lymphocytes Bioactivities and Histopathology in Broiler Chickens#br#
    LI Hong-mei,CHENG Zi-qiang,LIU Jian-zhu,LIU Fa-xiao,GUO Hui-jun,CUI Zhi-zhong
    Scientia Agricultura Sinica. 2009, 42(9):  3296-3304 .  doi:10.3864/j.issn.0578-1752.2009.09.036
    Abstract ( 930 )   PDF (631KB) ( 825 )   Save
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    【Objective】 To study the effect of single- and co-infection with REV and ALV-J on T lymphocytes bioactivities and histopathology in broiler chickens. 【Method】 Blood and spleen T lymphocytes bioactivities including lymphoproliferation responses and cytotoxicitic responses and pathological histopathology of spleen were detected in broiler chickens singl- or co-infected with REV and ALV-J at different days post inoculation, the viruses’ expression in infected broiler’s spleen were detected with immunofluorescence assay (IFA). 【Result】 The results indicate that, blood and spleen T lymphocytes proliferate responses and cytotoxicity in broilers infected with REV or/and ALV-J were inhibited in the whole observed period compared with controls, in the co-infected chickens they were inhibited more severely than in the single-infected. The histopathology of spleen in infected chickens at 17 and 37 days post inoculation indicated that cell interium increased, the numbers of lymphocytes decreased and the regrowth were destroyed or decreased, especially more significiant at 17dpi than at 37 dpi. The different numbers of virus were detected in spleen lymphocytes in REV-infected and/or ALV-J-infected chickens, respectively. In co-infected chickens’ spleen, both REV and ALV-J were detected and the total numbers of viruses were more than in chickens single-infected with REV or ALV-J. 【Conclusion】 It can be concluded that co-effect of REV and ALV-J can cause more immunosuppression on T lymphocytes bioactivities in broiler chickens than single-effect of ALV-J or REV, which contribute to the sever histopathology and the product of tumor cells. This will be helpful for understanding of the effect of co- or dulpti-infection with many viruses and control them in poultry.

    In vitro Differentiation of EGFP Gene Transfected Porcine Fetal Neural Stem Cells#br#
    ZHENG Yue-mao,ZHAO Xue,HE Xiao-ying,QUAN Fu-sheng,LIU Jun,ZHANG Yong
    Scientia Agricultura Sinica. 2009, 42(9):  3305-3313 .  doi:10.3864/j.issn.0578-1752.2009.09.037
    Abstract ( 889 )   PDF (595KB) ( 747 )   Save
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    【Objective】 Despite the increasing importance of the pig as a large animal model, little is known about the porcine neural stem cells (NSCs). 【Method】 To evaluate the markers expressed by NSCs, expression of EGFP by the cells and in vitro differentiation of the cells, brains were dissected from 30-day fetuses, enzymatically dissociated, and grown in the presence of epidermal growth factor, basic fibroblast growth factor etc. Porcine NSCs could be grown as suspended spheres. Plasmid containing EGFP gene was transfected into NSCs by lipofection and selected by G418. The positive NSCs were abtained. NSCs were induced to differentiate into cell types representing each embryonic germ layer, including cells of adipogenic, osteogenic, myogenic, endothelial, neuronal and chondrogenic lineages. 【Result】 Expanded NSCs and differentiated cells were banked or harvested for analysis using reverse transcription–polymerase chain reaction (RT-PCR). Cultured porcine NSCs widely expressed Nestin, NogoA, DCX, CyclinD2, CD133, Hes1, Oct4, CD-90, Nanog and Sox2. NSCs were differentiated into astrocyte (GFAP+), oligodendrocyte (GalC+), neuron (NF+、NSE+ and MAP2+), adipocyte (LPL+ and PPARγ-D+), osteoblast (Osteonectin+ and Osteocalcin+), myocyte (myf-5+, myf-6+ and myoD+), endothelium (CD31+, CD34+, CD144+ and eNOS+) and chondrogenic cells (COL2A1+). 【Conclusion】This study shows that porcine NSCs are broadly multipotent, thus, may be useful in porcine cell transplantation studies potentially leading to the application of this strategy in the setting of nervous system disease and injury.

    Effects of Fructus Arctii and Arctiin on cAMP and cAMP Phosphodiesterase Activity in Mouse Skeletal Muscle and on Growth Performance#br#
    GU Jin-ni,CHEN Wu,JIANG Dai-xun,ZHANG Bing,LIU Jie,YU Tong-quan,LU Ping,MU Xiang
    Scientia Agricultura Sinica. 2009, 42(9):  3314-3319 .  doi:10.3864/j.issn.0578-1752.2009.09.038
    Abstract ( 1121 )   PDF (299KB) ( 862 )   Save
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    【Objective】 To understand the effects and mechanism of Fructus Arctii and arctiin on promoting animal growth, cAMP PDE activity and cAMP content in skeletal muscle, plasma cAMP content, and growth performance of mouse were observed. 【Method】 ICR mice were intragastricly administered respectively with 1.00, 0.50 and 0.25 g/mouse of Fructus Arctii, 1.50, 0.75 and 0.38 mg/mouse of arctiin, and 0.75, 0.38 and 0.19 mg/mouse of theophylline which was used as a positive control. Body weight and food intake were recorded. Cyclic AMP PDE activity and cAMP content in skeletal muscle and plasma cAMP content were assayed by HPLC and ELISA. 【Result】 Food intake was significantly reduced by Fructus Arctii with 1.00 and 0.5 g and arctiin with three doses; body weight gain was noticeably increased by Fructus Arctii, arctiin and theophylline in all doses except 0.38 mg of arctiin; transform efficiency of food was extremely higher in all treated group than in control group (P<0.05, P<0.01 or P<0.001). Cyclic AMP PDE activity was significantly inhibited by all doses of Fructus Arctii, arctiin and theophylline; cAMP content in skeletal muscle was markedly increased in all groups except 1.50 mg of arctiin; plasma cAMP content was significantly higher than the control group except 0.75 mg of theophylline (P<0.05, P<0.01 or P<0.001). Moreover, cAMP content in skeletal muscle was correlated with cAMP PDE activity with r =-0.402 (P<0.001); plasma cAMP concentration was correlated with cAMP concentration with r =0.553 (P<0.001), and was correlated with cAMP PDE activity with r =-0.436 (P>0.05). 【Conclusion】 These results suggested that mouse growth performance was promoted by Fructus Arctii and arctiin through inhibiting skeletal muscle cAMP PDE activity and increasing skeletal muscle and plasma cAMP level.

    Preliminary Study on Serum Pharmacochemistry of Andrographis Paniculata in Chickens#br#
    LIU Kai-yong,GAO Hai,HUANG Xian-hui,HE Li-min,CHEN Jian-xin,CHEN Zhang-liu,ZENG Zhen-ling
    Scientia Agricultura Sinica. 2009, 42(9):  3320-3327 .  doi:10.3864/j.issn.0578-1752.2009.09.039
    Abstract ( 867 )   PDF (468KB) ( 935 )   Save
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    【Objective】 To study the serum pharmacochemistry of Andrographis paniculata in chickens firstly. 【Method】Based on the established HPLC-MS fingerprints of Andrographis paniculata ultra- fine powder,analysis and comparison were carried out among the HPLC-MS profiles of chicken serum samples obtained after oral administration and control serum, and then the compounds absorbed into blood, their original crude drugs and their metabolites were identified. 【Result】 Nine components were found in chicken serum after oral administration at 60 min, the four compounds of them ,the main ingredients, were originated from Andrographis paniculata and their higher level of serum consentrations maintained from 0-480 min; other compounds should be metabolites. 【Conclusion】 The compounds are absorbed into blood and their metabolites would be the effective constituents and their serum pharmacochemistry should be subject to complete investigation so as to lay a foundation for the further study of active ingredients of Andrographis paniculata.

    Preparation and Evaluation of O/W Pharmaceutical Microemulsions#br#
    LIU Gen-xin,ZHANG Ji-yu,WU Pei-xing,LI Jian-yong,LIU Ying,ZHOU Xu-zheng,WEI Xiao-juan,NIU Jian-rong,LI Bing
    Scientia Agricultura Sinica. 2009, 42(9):  3328-3333 .  doi:10.3864/j.issn.0578-1752.2009.09.040
    Abstract ( 1039 )   PDF (525KB) ( 1930 )   Save
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    【Objective】 The basic formation rules and evaluation of the pharmaceutical microemulsions were studied in order to provide some experimental information to studying and application of pharmaceutical microemulsions. 【Method】 The titrimetric method for drawing pseudo-ternary phase diagrams was used to inspect the formation influence caused by different surfactants and CO-surfactant on the O/W microemulsions. 【Result】 In the same oil phase situation, the formation of microemulsions would be influenced by the structure and nature of surfactant and CO-surfactant, the quality ratio (Km) of surfactant and CO-surfactan as well as the temperature. 【Conclusion】 Appropriate oil phase and suitable matches of the surfactant and CO-surfactant can reduce the amount of surfactant in the microemulsion system to 7.5%. Moreover, in general, the region size of the microemulsions has a positive relationship with the total amount of surfactant and CO-surfactant, and has a negtive relationship with the oil used. In order to use drawing pseudo-ternary phase diagrams to determine the most excellent formation condition of pharmaceutical microemulsions, the stability of microemulsions needs to be unified.

    RESEARCH NOTES
    Transformation of the Gene pf40 in Maize#br#
    LI Bo,YU Jing-juan,ZHAO Qian,ZHU Deng-yun,AO Guang-ming
    Scientia Agricultura Sinica. 2009, 42(9):  3334-3338 .  doi:10.3864/j.issn.0578-1752.2009.09.041
    Abstract ( 1038 )   PDF (303KB) ( 868 )   Save
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    【Objective】 Isolated from the cDNA library of millet immature seed, the gene pf40 (or its homolog ) is ubiquitously distributed in cereal’s crop genome. Preliminary studies indicate that the gene pf40 plays a role in promoting tillering (branching). This study aimed to clarify whether the function of gene pf40 (or its homolog) is related to the tillering in maize. 【Method】 Two plant expression vectors with sense and knock-out (RNAi) gene pf40 promoted by 35S promoter were introduced into the embryogenic callus of maize hybrids(Z3, Z31, Q31) through particle bombardment. Regenerated plants were verified to be transgenic by PCR amplification, Southern blot and tRNA dot blot. The observation about the phenotype of the tillers on the base of the transgenic plants stems could determine the effect of the gene pf40 on the tillers. 【Result】 After the hygromycin B selection and PCR screening, 53 transgenic plants were obtained. According to the results of PCR test, Southern blot and tRNA dot blot on T1 and T2 generation, the foreign gene pf40 had been integrated into the maize genome, also it could express and pass generation to generation. After investigated into the phenotypes of both the tiller nodes on the base of stems, unfortunately, no distinct difference was observed between the transgenic plants and the untransgenic plants. 【Conclusion】 The phenotype of the maize without tillers will not be changed by merely altering the expression of the gene pf40 or its homolog. And the exact functions of the gene pf40 or its homolog in maize need further to be investigated.

    Cloning, Characterization, and Expression Patterns Under Various Pi Levels of TaZAT6, a Zinc-Finger Protein Gene in Wheat (Triticum aestivum L.)#br#
    LI Xiao-juan,SUN Zhao-hua,KE Xin,LU Wen-jing,GUO Cheng-jin,GU Jun-tao,XIAO Kai
    Scientia Agricultura Sinica. 2009, 42(9):  3339-3345 .  doi:10.3864/j.issn.0578-1752.2009.09.042
    Abstract ( 1068 )   PDF (533KB) ( 629 )   Save
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    【Objective】 Based on the cloning of wheat zinc finger protein gene TaZAT6, the molecular characterization and the expression patterns under various Pi levels of this gene were studied. 【Method】 After sequencing of clones in a subtractive suppression cDNA library constructed from cv. Shixin828, in which the differential expressed genes in various deficient-Pi time points were enriched, a new EST of zinc finger protein type transcription factor was identified. The gene, TaZAT6, corresponding to this EST, was cloned using RT-PCR approach from cv. Shixin828 and Ji7369. Similarly, the expression patterns of TaZAT6 under various Pi conditions were analyzed with RT-PCR method. 【Result】 With an open reading frame of 717 bp, TaZAT6 encoded 238 amino acids, in which containing one conserved nuclear location signal (NLS), two C2H2 zinc-finger domains, and one conserved DLN box. Phylogenetic analysis suggested that TaZAT6 derived from same ancestor with other two zinc-finger protein gene ZAT22 and ZAT23 in wheat. The expression of TaZAT6 was shown to be Pi-deprivation inducible, and reduced to the level of pre-initiation of low-Pi treatment when resupply of normal phosphorus (2 mmol?L-1 Pi). Compared to those of Ji7369, a cultivar of low-P use efficiency, TaZAT6 in Shixin828 had much stronger capability of responding to the deficient-Pi treatment. TaPT2, one high-affinity phosphate transporters, displayed similar expression patterns with TaZAT6 when treated with low Pi, suggesting that its expression was partly regulated by TaZAT6 at the transcription level. 【Conclusion】 The stronger responding capability of TaZAT6 to low-Pi and its further transcriptional regulation of corresponding downstream genes, is possibly related to the high-P use efficiency of Shixin828 under deficient-Pi condition.

    Genetic Diversity of Peanut Landraces in Hebei Province Revealed by SSR Markers#br#
    CUI Shun-li,LIU Li-feng,CHEN Huan-ying,GENG Li-ge,MENG Cheng-sheng,YANG Yu
    Scientia Agricultura Sinica. 2009, 42(9):  3346-3353 .  doi:10.3864/j.issn.0578-1752.2009.09.043
    Abstract ( 1085 )   PDF (421KB) ( 1048 )   Save
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    【Objective】 In order to assess the genetic diversity of peanut landraces from Hebei province and provide a theoretical basis for peanut breeding. 【Method】 Genetic diversity of 75 accessions of peanut landraces with different botanical types, collected from Hebei province, was evaluated by 20 SSR molecular markers. 【Result】 Sixty-five alleles were detected. The average number of alleles per locus was 3.25 with a range from 2 to 6. Total Shannon information index of per locus varied widely from 0.1680 (7G02) -1.3617 (PM15) with an average value of 0.5448; Total Nei’s genetic diversity index of Nei per locus varied widely from 0.3385 (7G02) -0.9013 (PM384) with an average value of 0.6458; The accessions of A. hypogaea showed higher genetic diversity than those of A. vulgaris and A. fastigiata. Cluster analysis with UPGMA showed that the materials tested could be divided into 2 groups: the first group was A. vulgaris and A. fastigiata group, the second was A. hypogaea group and the genetic relationship among these landraces was not in accord with their geographical origins. 【Conclusion】 The peanut landraces from Hebei province showed higher genetic diversity revealed by SSR markers.
    Tillage Effects on Soil Respiration and Contributions of Its Components in Winter Wheat Field#br#
    ZHANG Yu,ZHANG Hai-lin,CHEN Ji-kang,CHEN Fu
    Scientia Agricultura Sinica. 2009, 42(9):  3354-3360 .  doi:10.3864/j.issn.0578-1752.2009.09.044
    Abstract ( 980 )   PDF (297KB) ( 1017 )   Save
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    【Objective】 Field experiment was carried out to estimate tillage effects on soil CO2 emission, relative contribution of root and soil respiration and carbon balance, and carbon respiration intensity during winter wheat growth season. 【Method】Conventional tillage (CT), rotary tillage (RT), and no tillage (NT) were used during 2006-2008, soil CO2 emission was measured by using static closed chambers and root contribution of root respiration was detected by root exclusion method. NEP was calculated for estimating carbon sequestration intensity. 【Result】 Soil CO2 emission was affected significantly by different tillage systems and it showed a significant seasonal fluctuating characteristic which was dropping until reached the lowest in hibernation and hoisting during the following period. The order of mean soil CO2 emission rate was CT>RT>NT. The mean ratio of root respiration contributed to total respiration was 26.18%, 29.96% and 36.44% under CT, RT and NT, respectively. The contribution ratio of rhizospheric respiration to total soil CO2 emission was fluctuated from 15% to 85% during the season and the ratio was highest at jointing stage of winter wheat under each tillage system. 【Conclusion】 Therefore, soils were sinks of atmospheric CO2 under each tillage system, but the order of the intensity of carbon sink was NT>RT>CT.

    Visualization-Based Growth Simulation System for Super-Maize#br#
    CHEN Guo-qing,ZHANG Ji-wang,DONG Shu-ting
    Scientia Agricultura Sinica. 2009, 42(9):  3361-3367 .  doi:10.3864/j.issn.0578-1752.2009.09.045
    Abstract ( 886 )   PDF (414KB) ( 1241 )   Save
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    【Objective】 This study aimed to show the whole growth process of super-maize and quantitate the development parameter. 【Method】 By applying the system analysis principle and mathematical modeling technique to the development of super-maize, the fundamental relationships and quantitative algorithms between the growth and development and morphogenesis of super-maize was analyzed. The virtual model for super-maize was developed with OpenGL on the basis of morphogenesis parameter, with use of Microsoft Visual C++ 6.0 to develop the system. 【Result】 The quantitative output and visualization expression of the growth and development parameter for super-maize were realized by using GDD as driving parameter. The prediction function of the system can be integrated easily with visualization expression on the basis of the data exchange of the morphogenesis model and growth model. 【Conclusion】 The flexibility and suitability of the system can be applied to super-maize, and this system will provide a technology support for the study of super-maize.

    Colchicine Chemical Induction During Somatic Embryogenesis from Leaf Segments of Phalaenopsis Test Tube Plantlets #br#
    CUI Guang-rong,ZHANG Zi-xue,ZHANG Cong-yu,HU Neng-bing,SUI Yi-hu,LI Jie-qin
    Scientia Agricultura Sinica. 2009, 42(9):  3368-3373 .  doi:10.3864/j.issn.0578-1752.2009.09.046
    Abstract ( 897 )   PDF (302KB) ( 898 )   Save
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    【Objective】 The leaf segments of in vitro Phalaenopsis were treated with colchicine for ployploid induction.【Method】 The effects of different concentrations and acting time of colchicines on the protocorm-like body (PLB) formation and shoot regeneration from the segments of in vitro Phalaenopsis leaves were studied. The morphological and histological characters of lower epidermal cell of leaves of the polyploid and diploid plantlets were compared. Meanwhile, the chromosomes of root tip cell of polyploid plantlets were identified. 【Conclusion】 The results showed that colchicines greatly affected PLBs formation and shoot regeneration from leaf segments of Phalaenopsis in vitro, and the rate of PLB formed and the number of shoots were obviously decreased with the increasing of the concentration of colchicines. However, the rate of polyploid plantlets regenerated was higher in the treatments of higher concentrations and longer acting time of colchicines. Morphologically, the polyploid plants were short and stout with rough and thicker leaves, but the root number of the plants was fewer, and shorter and thicker than the diploid. The structure of lower epidermal tissue, cell and stomata were obviously different between the diploid and polyploid. The nucleus of the polyploid cell was bigger and the number of the chromosome was doubled.

    Correlations Among Shearing Force, Morphological Characteristics, Chemical Compositions, and in situ Degradibility of Alfalfa Stem and Rye Grass Stem#br#
    LIU Li,YANG Zai-bin,YANG Wei-ren,JIANG Shu-zhen,ZHANG Gui-guo,YAO Bao-qiang
    Scientia Agricultura Sinica. 2009, 42(9):  3374-3380 .  doi:10.3864/j.issn.0578-1752.2009.09.047
    Abstract ( 1160 )   PDF (260KB) ( 676 )   Save
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    【Objective】 The objectives of this study were to investigate the relationships of shearing force with morphological characteristics, chemical compositions, and in situ degradability in 48 h of alfalfa (Medicago sativa L.) stem and Dongmu-70 rye grass (Secale cereale L.) stem. 【Method】 The diameter, length,weight and linear density of stem were measured after sample collection. Shearing force was measured. Meanwhile, the content of moisture were measured, neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin, cellulose and hemicellulose were measured. Three Xiaowei sheep were used to determine in situ degradability in 48 h of DM and NDF of alfalfa stem and rye grass stem. 【Result】 The results showed that linear density, shearing force and lignin content of alfalfa stem increased as diameter increased. There were positive relationships of shearing force with diameter, linear density, and lignin content of alfalfa stem (P<0.01). A negative correlation was found between shearing force and moisture content (P<0.01). Negative correlations also were found between shearing force and DM degradability, NDF digestibility of alfalfa stem (P<0.05). Linear density, stem thickness, ADF, lignin and moisture content increased when rye grass stem increased. There were positive relationships of shearing force with diameter, linear density, stem thickness, ADF, lignin and moisture content of rye grass stem (P<0.05). In situ degradability of rye grass stem decreased with increasing shearing force, but the linear correlations were not significant (P>0.05). 【Conclusion】 The results showed that the variance in stem shearing force of Alfalfa and rye grass are due to a combination of factors, including changes in chemical composition, morphological characteristics. DM and NDF degradability of alfalfa stem had negative relationships with shearing force. Shearing force could be used as an indicator of forage stem nutritive value. There were no negative relationships between shearing force and DM, NDF degradability of rye grass.