Abstract:

【Objective】 Changes of protein expression were investigated in mammary gland from healthy dairy cows and clinical mastitic cows in order to detect the disease-related proteins and understand molecular mechanism of clinical mastitis at the protein level. 【Method】 The project presents the protein changes in mammary gland using two-dimensional gel electrophoresis (2-DE), after stained with colloidal coomassie blue, differential expression proteins were detected by PDQuest 7.4 software and subjected to ion trap mass spectrometer equipped with a Surveyor HPLC system, differential spots of protein were identified. 【Result】 Fifteen protein spots were differentially expressed, and twelve proteins were identified after SEQUEST research and corresponding to ten proteins. Overall, these differential proteins were involved in molecular function contained binding, transport and catalytic activity; and participated in biological process contained cellular process, metabolic process, biological regulation and so on. 【Conclusion】 There was a significant difference at protein level in mammary gland from healthy dairy cows and clinical mastitic cows, differential proteins were related to infection of mastitis. It suggests that these proteins may be useful to explore the mammary gland involved in the pathophysiology, and to reveal the molecular mechanisms of clinical mastitis.

Key words: dairy cows, mastitis, mammary gland, proteome