Scientia Agricultura Sinica ›› 2008, Vol. 41 ›› Issue (6): 1845-1850 .doi: 10.3864/j.issn.0578-1752.2008.06.036

• RESEARCH NOTES • Previous Articles     Next Articles

Cloning and Sequence Analysis of α-Gliadin Genes from Spelt Wheat

  

  1. 四川农业大学农学院
  • Received:2005-10-19 Revised:2006-03-26 Online:2008-06-10 Published:2008-06-10

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Abstract: Specific PCR primer were designed based on the known α-gliadin gene sequences, and the coding regions of two gliadin genes Spelt-Gli-1 and Spelt-Gli-2 were isolated from spelt wheat (Triticum spelta L.), among which Spelt-Gli-1 was a pseudogene due to the stop codons in its coding region. The full coding region of Spelt-Gli-2 was 849bp, and could be translated into a protein of 263 amino acids. The two cloned gliadin genes had the typical structure of α-gliadin genes. The deduced amino acid sequences comparison suggested that Spelt-Gli-2 had a high degree of identity with the known a-gliadin genes.

Key words: spelt wheat, α-gliadin, gene cloning, sequences analysis

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