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Review and Prospect of Huanglongbing Research Project

ZHAO Xue-yuan, JIANG Yuan-hui   

  1. Citrus Research Institute, Chinese Academy of Agricultural Sciences/Citrus Research Institute, South West University, Chongqing 400712
  • Online:2007-12-31 Published:2007-12-31

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Abstract: The survey of the occurrence of huanglongbing (HLB) indicated that in the mainland of China, HLB is not only prevalent in Guangdong, Fujian and Guangxi ,but also in southern Zhejiang, southern Jiangxi, some areas of Southern Hunan, a part of Guizhou, Yunnan and some areas of Sichuan neighbouring Yunnan. In the survey, mottling yellow leaf is commonly used as main cristerion for HLB diagnosis in the field. The tetracycline sensitivity of the causal agent of HLB was experimentally confirmed in 1974-1975, and mycoplasma-like organism etiology of HLB was preliminarily declared. Immersing budwood in tetracycline was recommended for producing HLB free budlings. The transmission of HLB by citrus psyllid, Diaphorina citri Kuw. and the exclusion of HLB pathogen by shoot-tip grafting have been experimentally confirmed. In the graft transmission tests, as the piece of stem bark is used as inoculum, the transmission rate is much lower than that as the bud or piece of stem is used. As budwoods are collected from the same diseased tree, and grafting taken in each month of one year, HLB incidence of the propeny grafted in May-July is apparently lower than that grafted in other periods. Scientific research cooperation, scientific popularization and international exchange in HLB research have been organized and/or joined. Further examination on HLB prevailing area in China, and further study on the rule of HLB transmission by citrus psyllid, the distribution of HLB bacterium in the host and the incubation period are prospected. The study on the isolation and culture of HLB bacterium and breeding of resistant or tolerant citrus by gene engineering is also prospected.

Key words: Citrus , Huanglongbing , Mycoplasma-like organism , Citrus psyllid (Diaphorina citri Kuwayama)

[1]陈其儤. 潮柑黄龙病研究报告. 新农季刊, 1943, 3(3-4): 142-175.
[2]林孔湘. 柑桔黄梢（黄龍）病研究Ⅱ: 关于病原的探讨. 植病学报, 1956, 2(1): 13-42.
[3]Matsumoto T, Wang M C, Su H J. Studies on Likubin. Proceedings of the Second Conference of IOCV, 1961: 121-125.
[4]Capoor S P. Decline of citrus trees in India. Bulletin National Institute of Science Indian, 1963, 24: 48-64.
[5]陈延熙, 梅汝鸿. 柑桔黄龙病毒研究初报. 植物保护学报, 1965, 4(4): 361-366.
[6]林孔湘. 对柑桔黄梢(黄龍病)病究竟要不要检疫？(初稿)与陈延熙教授商榷. 内部资料, 1963.
[7]Doi Y, Teranaka M, Yora K, Asuyama H. Mycoplasma or PLT group-like microorganisms found in the phloem elements of plants infected with mulberry dwarf,potato witches’broom. Annual Phytopathology Society, 1967, 33: 259-266.
[8]Ishiie T, Doi Y, Yora K, Asuyama H. Suppressive effects of antibiotics of tetracycline group on symptom development of mulberry dwarf disease. Annual Phytopathology Society, 1967, 33: 267-275.
[9]Lafleche D, Bove J M. Mycoplasmes dans les agrumes atteints de “greening”, de “stubborn”ou de maladie du similaires. Fruits, 1970, 25: 455-465.
[10]Chen M H, Miyakawa T, Matsui C. Mycoplasmalike bodies associated with likubin-diseased Ponkan citrus. Phytopathology, 1971, 61(5): 598.
[11]Tanaka S, Doi Y. Mycoplasma-like organism found in citrus plants infected with likubin and leaf mottling. In: International Citrus Congress, Murecia-Valencia. 1973: 352-353.
[12]Martinez A L, Nora D M, Armedilla A L. Suppression of symptoms of citrus greening disease in the Philippines by treatment with tetracycline antibiotics. Plant Disease Reporter, 1970, 54(12): 1007-1009.
[13]Schwarz R E, van Vuuren S P. Decrease in fruit greening of sweet orange by trunk injection of tetracyclines. Plant Disease Reporter, 1971, 55(8): 747-749.
[14]Nienhaus F, Sikora R A. Mycoplasmas, spiroplasmas, and rickettsia-like organisms as plant pathogen. Annual Review Phytopathology, 1979, 17: 37-58.
[15]McClean A P D, Oberholzer P C J. Citrus psylla, a vector of the greening disease of sweet orange. South Africa Journal of Agricutureal Science, 1965, 8: 297-298.
[16]Capoor S P, Rao D G, Viswanath S M. Diaphorina citri Kuway, a vector of the greening disease of citrus in India. Indian Journal of Agricultural Science, 1967, 37: 572-576.
[17]Martinez A L, Wallace J M. Citrus leaf mottle-yellows disease in the Philippines and transmission of the causal virus by a psyllid, Diaphorina citri. Plant Disease Reporter, 1967, 51: 692-695.
[18]Garnier M, Danel N, Bove J M. Aetiology of citrus greening disease. Annual of Microbiology (Institute Pasteur), 1984, 135A: 169-179.
[19]Jagoueix S, Bove J M, Garnier M. PCR detection of the two ‘Candidatus’ liberobactor species associated with greening disease of citrus. Molecular Cellular Probes, 1996, 10: 43-50.
[20]Texeira D C, Ayres A J, Kitajima E W, Tanaka F A O, Danet J L, Jagoueix-Eveillard S, Saillard C, Bove J M. First report of a Huanglongbing-like disease of citrus in Sao Paulo State, Brazil, and association of a new liberibacter species, “Candidatus Liberibacter americanus”, with the disease. Plant Disease, 2005, 89: 107.
[21]曾  勉, 赵学源. 对柑桔黄龙病防治的初步意见. 中国农业科学, 1963, (7): 17-20.
[22]广西柑橘黄龙病研究小组. 广西柑桔黄龙病初步调查. 中国农业科学院柑桔研究所科学研究年报, 1963: 179-204.
[23]广西柑桔黄龙病研究小组. 柑桔木虱和柑桔黄龙病.柑桔科技通讯, 1977, (3-4): 23-24.
[24]赵学源, 蒋元晖, 李世菱, 邱柱石, 苏维芳. 柑桔木虱 ( Diaphorina citri Kuwayama) 与柑桔黄龙病流行关系的初步研究. 植物病理学报, 1979, (2): 121-126.
[25]四川柑桔黄龙病调查小组. 西昌地区和渡口市柑桔黄龙病发生情况调查简报. 柑桔科技通讯, 1977, (3-4): 21-22.
[26]Zhao X Y. Citrus yellow shoot disease (Huanglongbing) in China—A Review. Proceedings of the International Society of Citriculture, 1981, 1: 466-469.
[27]云南柑桔黄龙病调查组. 云南柑桔黄化问题考察报告. 中国柑桔, 1981, (4): 14-16.
[28]蒋元晖, 邱柱石. 一个值得重视的问题——略谈近年柑桔黄龙病区的增加. 中国柑桔, 1982, (4): 17-18.
[29]Zhao X Y, Jiang Y H. The present status of citrus virus and virus-like diseases in China. Fruit Varieties Journal, 1985, (3):30-35.
[30]Zhao X Y. Occurrence of citrus yellow shoot (greening) disease in Guangxi and southern Sichuan—A brief review. In: FAO-UNDP Reginal Workshop on citrus greening, huanglongbing disease, Reviews and Abstracts, Fuzhou-China, 1987.
[31]Zhao X Y, Jiang Y H, He X H, Zhou C Y, Chen J Z, Ouyang L, Li S L, Su W F, Huang T Y, Huang Z Q. Occurrence and distribution of citrus virus and virus-like diseases in the mainland of China. In: Proceedings of the International Citrus Symposium, Guangzhou, China, 1990: 547-552.
[32]Zhao X Y. Citrus production and the occurrence of virus diseases in the mainland of China. In: Proc. 13th Conf. IOCV., IOCV., Riverside, 1996: 8-11.
[33]高与檉, 黄荣玉, 龚钧智. 柑桔黄龙病的接种和预防试验. 植物保护学报, 1963, 2(3): 347-349.
[34]赵学源, 蒋元晖, 朱伟生. 几种柑桔类实生苗嫁接接种柑桔黄龙病毒的反应初报. 见: 中国农业科学院柑桔研究所专题研究报告. 1965, 桔研64-8.
[35]广西柑桔黄龙病研究小组. 广西柑桔黄龙病防治研究小结(1963-1971). 见: 广西柑桔黄龙病研究小组资料, 1972.
[36]赵学源, 蒋元晖, 邱柱石, 李柏梅. 柑桔黄龙病病株感染衰退病毒情况的鉴定. 中国柑桔, 1979, (3): 33-34.
[37]广西柑桔黄龙病研究小组. 利用四环素、土霉素治疗柑桔黄龙病的效果(初报). 柑桔科技通讯, 1975, (1): 31-34.
[38]广西柑桔黄龙病研究小组. 柑桔黄龙病病原及防治的初步研究. 中国农业科学, 1978, (3): 84-86.
[39]柯  冲, 林先沾, 陈  辉, 陈元忠, 章连钧. 柑桔黄龙病与类立克次体及线状病毒的研究初报. 科学通报, 1979, (10): 463-466.
[40]陈作义, 沈菊英, 陶世珍, 龚祖埙, 陈乃荣, 戴月明. 柑桔黄龙病病原体的研究Ⅲ. 广东柑橘黄龙病病树中的类菌质体病原. 生物化学与生物物理学报, 1979, 11(2): 191-192.
[41]陈作义, 沈菊英, 陶世珍, 龚祖埙, 赵学源, 蒋元晖, 邱柱石. 广西柑桔黄龙病株中类菌原体病原的发现. 生物化学和生物物理学报, 1979, (4): 389-390.
[42]陈作义, 沈菊英, 龚祖埙, 陈乃荣, 戴月明. 柑桔黄龙病病原体及其对抗生素反应的研究. 生物化学与生物物理学报, 1980, 12(2): 143-146.
[43]赵学源, 邱柱石, 苏维芳, 蒋元晖. 柑桔黄龙病对青霉素和四环素的反应. 中国柑桔, 1981, (4): 17.
[44]华南农学院植保系植病教研组. 柑橘木虱传递柑橘黄龙病试验初报. 广东农业科学, 1977(6): 50-51, 53.
[45]赵学源, 蒋元晖, 邱柱石, 苏维芳, 李超扬. 柑桔黄龙病嫁接方法的初步研究. 植物病理学报, 1982, (2): 53-56.
[46]赵学源, 邱柱石, 苏维芳, 蒋元晖. 四环素浸泡芽条消除柑桔黄龙病的持续效果. 植物保护学报, 1982, (1): 67-68.
[47]蒋元晖, 赵学源, 苏维芳, 黄通意, 黄志巧. 通过茎尖嫁接脱除柑桔黄龙病病原体. 植物保护学报, 1987, (3): 184, 162.
[48]陈  駉, 宋瑞琳, 柯  冲. 在试管中茎尖嫁接脱除柑桔黄龙病病原的研究. 福建省农科院学报, 1987, 2(1): 67-71.
[49]龙钦贤, 庄娴兰, 邱柱石, 苏维芳, 赵学源. 加压注射四环素族抗生素治疗甜橙黄龙病树的效果. 中国柑桔, 1981, (1): 29-33.
[50]Zhao X Y. Huanglongbing in China. Proceedings of the Huanglongbing-greening International Workshop, Ribeirao Preto, SP. Brazil, 2006: 3.
[51]Zhao X Y. Huanglongbing management in China. In: Proceedings of the Huanglongbing-greening International Workshop, Ribeirao Preto, SP. Brazil, 2006: 41.
[52]赵学源, 蒋元晖. 广西柑桔黄龙病研究小组工作回顾. 广西柑桔, 1996, (1): 9-13; (2): 26-30.
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