Abstract: The purpose of this experiment was to study the effect of hypoxia on the Ca2+ concentration in broiler’s Cardiac Muscle Cell (CMC) . The concentration of Ca2+ in the CMC was observed by laser scanning confocal microscope (LSCM). The results showed that hypoxia could significantly increase intracellular Ca2+ of CMC (CMC: normal oxgen:99.3±13.1, hypoxia:129.4±24.3,p<0.01.). The Ca2+ antagonist (nifedipine, Nif; verapamil, Ver) can significantly restrain the Ca2+ influx across the cell membrane of CMC treated by hypoxia (CMC: hypoxia + Ver:100.9±28.2, hypoxia＋Nif:107.6±27.7, p<0.01). Hypoxia is the important cause of ascites syndrome（AS）, myocardial hypertrophy is the typical pathological change .The results suggest that the transmembrane movement of Ca2+ in the CMC maybe play an important role in the development of AS .

Key words: Broiler Chicke, Cardiac Muscle Cell, Ca2+, Hypoxia