Abstract:

【Objective】 Intercelluar washing fluid（IWF-260）of leaf rust - infected wheat leaves ( Lovrin 10 ) was used to stimulate the suspension cell of Lovrin 10 in order to study the H2O2 burst and its productive mechanism. 【Method】 The changes of H2O2 were detected by chemiluminescence method and fluorescent probe DCFHDA staining. The activity of NADPH oxidase was studied by HE staining. Pharmacological experiments were carried out to study the relation between Ca2+ and H2O2。【Result】IWF-260 could cause the outbreak of H2O2 in wheat suspension cultured cells. It played an important role in the programmed death of wheat suspension cells induced by IWF-260. NADPH oxidase might be involved in the production of H2O2. The influx of extracellular Ca2 + could regulate the production of H2O2. 【Conclusion】 IWF can induce H2O2 burst of wheat suspension cells and this process is involved with the activation of NADPH oxidase and the influxing of intercelluar Ca2+.

Key words: H2O2')">H2O2, intercelluar washing fluid (IWF), plasma membrane NADPH oxidase, Ca2+