Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (19): 4040-4049.doi: 10.3864/j.issn.0578-1752.2012.19.016

• HORTICULTURE • Previous Articles     Next Articles

Ultracytochemical Localization and Effects of Calcium on Isoenzyme in the Leaves of Cucumis melo L. Under the Stress of Powdery Mildew

 ZHONG  Li, LI  Guan   

  1. 1.新疆大学生命科学与技术学院，乌鲁木齐 830046
  • Received:2012-05-14 Online:2012-10-01 Published:2012-08-01

PDF

1014

Cited

2

Abstract: 【Objective】The aim of this experiment is to study the uhracytochemical localization of calcium in mesophyll cells of Cucumis melo L. before and after inoculating Podosphaera xanthii and the effects of exogenous calcium on three isoenzymes of defense enzymes which will be useful for further elucidating the process of calcium signal transduction and resistance mechanism to powdery mildew of melon. 【Method】With the method of electron microscope and cytochemistry technology, a hydroponic experiment was carried out to explore the effects of different calcium treatments on isoenzymes of peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) in leaves of the melon seedlings under powdery mildew stress．【Result】The calcium in mesophyll cells of resistant variety ‘MR-1’ and in susceptible variety ‘JS’ gathered in cytoplasm and there were a remarkable increase of calcium level in cytoplasm and reduced greatly in vacuolate and intercellular space when inoculating P. xanthii for 2 d. The calcium localization in leaf cells of ‘MR-1’ trended to the status as before inoculating when the seedlings were treated for 6 d. However, larger calcium deposited and localized together in the cytoplasm of ‘JS’ and the structure of mesophyll cells were destroyed until to death and there were no recover of calcium from cytoplasm into vacuolate and intercellular space. Compared with the control, the application 6 mmol•L-1 CaCl2 in nutrient solution significantly alleviated the stress on the melon leaves system that the activities of SOD, POD and CAT isoenzymes were significantly improved, while application of 75 mmol•L-1 LaCl3 in nutrient solution significantly inhibited the three isoenzymes activities.【Conclusion】Calcium ditribution in leaf cells of C. melo L. changed greatly due to its resistance ability at the stress of powdery mildew. A lot of Ca2+was released from Ca2+ pool (vacuoles) into cytoplasm, then was pumped return vacuoles in mesophyll cells of resistance variety ‘MR-1’, but in mesophyll cells of susceptible variety ‘JS’, there only occurred the first stage. Exogenous calcium probably increased calcium transport to the plant, improved the signal transmission to the body, and enhanced the scavenging of reactive oxygen species in plant cell under stress．Thereby, exogenous calcium enhanced the ability of powdery mildew resistance of melon plants.

Key words: Cucumis melo L., stress of powdery mildew, cellular cytochemistry localization, Ca2+, isoenzyme

[1]汤  谧, 别之龙, 张保才, 吴明珠, 伊鸿平, 冯炯鑫. 西瓜、甜瓜果实品质及调控研究进展. 长江蔬菜, 2009, 2b: 10-14.

Tang M, Bie Z L, Zhang B C, Wu M Z, Yi H P, Feng J X. Progress of fruit quality and control in watermelon and melon. Journal of Changjiang Vegetables, 2009, 2b:10-14. ( in Chinese)

[2]赵光伟, 徐永阳, 徐志红, 邢  燕. 甜瓜抗白粉病基因SRAP分子标记筛选. 西北植物学报, 2010, 30(6): 1105-1110.

Zhao G W, Xu Y Y, Xu Z H, Xing Y. A novel SRAP marker of the resistant gene for powdery mildew in melon. Acta Botanica Boreali-Occidentalis Sinica, 2010, 30(6): 1105-1110. (in Chinese)

[3]Cheng H, He Q W, Huo Y M, Hou L X．Molecular cloning, characterization and expression analysis of CmAPX. Plant Molecular Biology Reporter, 2009, 36: 1531-1537.

[4]孙大业, 郭艳林, 马力耕, 崔素娟. 细胞信号转导(3版). 北京: 科学出版社, 2001.

Sun D Y, Guo Y L, Ma L G, Cui S J. Cellular Signal Transduction (3rd ed.). Beijing: Science Press, 2001. (in Chinese)

[5]Knight H, Trewavas A J, Knight M R. Cold calcium signaling in Arabidopsis involves two cellular pools and a change in calcium signature after acclimation. The Plant Cell, 1996, 8: 489-503.

[6]Knight H, Trewavas A J, Knight M R. Calcium signaling in Arabidopsis thaliana responding to drought and salinity. The Plant Journal, 1997, 12(5): 1067-1078.

[7]Evans N H, Mcainsh M R, Hetherington A M, Knight M R. ROS perception in Arabidopsis thaliana: the ozone-induced calcium response. The Plant Journal, 2005, 41(4): 615-626.

[8]Clapham D E． Calcium signaling．Cell, 1995, 80: 259-279．

[9]胡晓辉, 郭世荣, 李  璟, 王素平, 贾永霞. 低氧胁迫对黄瓜幼苗根系无氧呼吸酶和抗氧化酶活性的影响. 武汉植物学研究, 2005, 23(4): 337-341.

Hu X H, Guo S R, Li J, Wang S P, Jia Y X. Effects of hypoxia stress on anaerobicrespiratory enzyme and antioxidant enzyme activities in roots of cucumber seedlings. Journal of Wuhan Botanical Research, 2005, 23(4): 337-341. (in Chinese)

[10]王长义, 郭世荣, 刘超杰. 钙对根际低氧胁迫下黄瓜幼苗根系保护酶同工酶表达的影响. 西北植物学报, 2009, 29(9): 1874-1880.

Wang C Y, Guo S R, Liu C J. Effects of calcium on expression of defense enzymes isoenzymes in roots of cucumber seedlings under root-zone hypoxic stress. Acta Botanica Boreali-Occidentalis Sinica, 2009, 29(9): 1874-1880. (in Chinese)

[11]王  迪, 田丽美, 李德泽, 李志学, 胡溪熙, 韩  墨. 甜瓜白粉病苗期接种方法和接种浓度的研究. 北方园艺, 2010(11): 185-186.

Wang D, Tian L M, Li D Z, Li Z X, Hu X X, Han M. Inoculation method and inoculum concentration of melon powdery mildew at seedling stage. Northern Horticulture, 2010(11): 185-186. (in Chinese)

[12]Mahajan S, Pandet G K, Tuteja N. Calcium-and salt-stress signaling in plants：shedding light on SOS pathway. Archives of Biochemistry and Biophysics, 2008, 471:146-158.

[13]王  红, 简令成, 张举仁. 低温胁迫下水稻幼叶细胞内 Ca2+水平的变化. 植物学报, 1994, 36(4): 587-591.

Wang H, Jian L C, Zhang J R. Changes of the Ca2+ level of in cells of rice seedlings under low temperature stress. Acta Botanica Sinica, 1994, 36(4): 587-591. ( in Chinese)

[14]杨海艳, 罗忠泽, 王昆林, 郭忠艳, 邱  璐, 范树国. 激光加电场处理水稻幼苗POD及CAT同工酶酶谱分析. 安徽农业科学, 2009, 37(11): 4892-4893, 4914.

Yang H Y, Luo Z Z, Wang K L, Guo Z Y, Qiu L, Fan S G. Study on lsoenzymes patterns of POD and CAT in rice seedlings treated by laser light and electric field. Journal of Anhui Agriculture Science, 2009, 37(11): 4892-4893, 4914. (in Chinese)

[15]罗广华, 王爱国. 植物SOD的凝胶电泳及活性的显示. 植物生理学通讯, 1983(6): 44-45.

Luo G H, Wang A G. Electrophoresis pattern of gel and active display for SOD of plant. Plant physiology communications, 1983(6): 44-45. (in Chinese)

[16]Knight M R, Campbell A K. Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium. Nature, 1991, 352: 524-530.

[17]Bush D S. Calcium regulation in plant cells and its role in signaling. Annual Review of Plant Biology, 1995, 46: 95-122.

[18]John J G, Gary J L. Role of reactive oxygen intermediates and cognate redox signaling in disease resistance. Plant Physiology, 2000, 124: 21-30.

[19]Apel K, Hirt H. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 2004, 55: 373-399.

[20]Wang W M, Wen Y Q, Berkey R, Xiao S Y. Specific targeting of the Arabidopsis resistance protein RPW8.2 to the interfacial membrane encasing the fungal haustorium renders broad-spectrum resistance to powdery Mildew. The Plant Cell, 2009, 21(9): 2898-2913.

[21]简令成, 王  红. Ca2+在植物细胞对逆境反应和适应中的调节作用. 植物学通报, 2008, 25(3): 255-267.

Jian L C, Wang H. Ca2+ Signaling in plant cell response and adaptation to lowtemperature, drought and salt stresses. Chinese Bulletin of Botany, 2008, 25(3): 255-267. (in Chinese)

[22]刘宏涛, 李 冰, 周人纲. 钙-钙调素信号系统与环境刺激. 植物学通报, 2001, 18(5): 554-559.

Liu H T, Li B, Zhou R G. Calcium -calmodulin signal transduction pathway and environment stimulation. Chinese Bulletin of Botany, 2001, 18(5): 554-559. (in Chinese)

[23]赵  昕, 裴真明, 何奕昆. 胞外Ca2+信号—动植物中的第一信使. 遗传, 2007, 29(3): 269-275.

Zhao X, Pei Z M, He Y K. Extracellular Ca2+ signaling-first messenger in animals and plants. Hereditas, 2007, 29(3): 269-275. (in Chinese)

[24]Morard P, Silvestre J. Plant injury due to oxygen deficiency in the root environment of soilless culture: a review. Plant and Soil, 1996, 184: 243- 254.

[25]Mori I C, Schroeder J I．Reactive oxygen species activation of plant Ca2+channels．A signaling mechanism in polar growth, hormone transduction, stress signaling, and hypothetically mechanotransduction. Plant Physiology, 2004, 135: 702-708.
[1] HUANG Cui-Xiang, XIA Yan-Fei, WANG Rong, ZHANG Wen-Hui, HAN Tian-Tian, SHEN Xiang. Effects of Phenanthrene and Pyrene on Ca2+, K+ and H+ Liquidity in Root Hair Cells of Malus hupehensis [J]. Scientia Agricultura Sinica, 2013, 46(20): 4321-4327.
[2] WANG Ming-Xia, ZHOU Zhi-Feng, YUAN Ling, HUANG Jian-Guo. Effects of Ca2+ Signal Inhibitors on Oxalate Effluxes by Ectomycorrhizal Fungi Under Aluminum Stress [J]. Scientia Agricultura Sinica, 2012, 45(5): 902-908.
[3] XIAO Yun-Qi, HUANG Yan-Bing, WU Jing, NI Ying-Dong, ZHAO Ru-Qian. Effect and Mechanism of Kisspeptin-10 on Progesterone Secretion in Granulosa Cells of Chicken F1 Follicles Cultured in Serum-Free Medium [J]. Scientia Agricultura Sinica, 2012, 45(2): 391-398.
[4] SONG Hai-Bin, CUI Xi-Bo, MA Hong-Yan, ZHU Zi-Cheng, LUAN Fei-Shi. Construction of DNA Fingerprint Database Based on SSR       Marker for Varieties(Lines) of Cucumis melo L. [J]. Scientia Agricultura Sinica, 2012, 45(13): 2676-2689.
[5] WANG Yun-Fei, BI Yang, REN Ya-Lin, WANG Yi, FAN Cun-Fei, LI Da-Qiang, YANG Zhi-Min. Control of Postharvest Diseases and Potentiation of Reactive Oxygen Species Metabolism in Muskmelon (Cucumis melo L.) Fruits Treated by Sodium Silicate [J]. Scientia Agricultura Sinica, 2012, 45(11): 2242-2248.
[6] . Flower Induction and Utilization of Zea luxurians in Maize Breeding

[J]. Scientia Agricultura Sinica, 2011, 44(6): 1109-1116 .
[7] ZHENG Ming-Xue, GU Shao-Peng, WANG Li-Xia, HAN Ke-Guang, LI Bao-Jun. Effect on E. tenella Invasion into Host Cells in Vitro by Inhibiting Calcium Signal Transduction [J]. Scientia Agricultura Sinica, 2011, 44(15): 3272-3278.
[8] REN Li-mei,CHEN Yan,WANG Dong-mei
. Induction of H2O2 Burst of Wheat Suspension Cells by IWF and Preliminary Study on Its Productive Mechanism#br# [J]. Scientia Agricultura Sinica, 2010, 43(8): 1577-1584 .
[9] YANG Feng-jun,LI Tian-lai,ZANG Zhong-jing,LU Shao-wei
. Effects of Timing of Exogenous Calcium Application on the Alleviation of Salt Stress in the Tomato Seedlings#br# [J]. Scientia Agricultura Sinica, 2010, 43(6): 1181-1188 .
[10] SUN Qing-peng,YU Yong-kun,WAN Shan-xia,ZHAO Fu-kuan,HAO Yu-lan
. Extracellular and Intracellular Calcium both Involved in the Jasmonic Acid Induced Calcium Mobilization in Arabidopsis thaliana
 [J]. Scientia Agricultura Sinica, 2010, 43(5): 942-948 .
[11] CHEN Nian-lai,HU Min,QIAO Chang- ping,NAI Xiao-ying,WANG Rui
. Effects of BTH, SA, and SiO2 Treatment on Disease Resistance and Leaf HRGP and Lignin Contents of Melon Seedlings
 [J]. Scientia Agricultura Sinica, 2010, 43(3): 535-541 .
[12] ZHANG Zhen-hua,LIU Qiang,SONG Hai-xing,RONG Xiang-min,Abdelbagi M. Ismail
. The Salinity Tolerance of Rice (Oryza sativa L.) Genotypes as Affected by Nutrients (K+, Ca2+ and Mg2+) at Seedling Stage
 [J]. Scientia Agricultura Sinica, 2010, 43(15): 3088-3097 .
[13] WANG Yan,WU Zhen-yu,DU Yan-li,SHI Guang-lu,CHEN Mei,WANG Hai-xiang,WANG You-nian
. Acaricidal Activity of an Extract of Pharbitis purpurea seeds Against Tetranychus cinnabarinus
 [J]. Scientia Agricultura Sinica, 2009, 42(8): 2793-2800 .
[14] HAO Jing-hong,LI Tian-lai,DU Zhe,KAN Su-e. Study on the Response of Endogenous Hormone to Different Night Temperature and Its Relationship with Fruit Development in Melon#br# [J]. Scientia Agricultura Sinica, 2009, 42(7): 2442-2448 .
[15] . Calcium Distribution in Anther of Photo-sensitive Genic Male-Sterile Rice and Its Relationship with Pollen Fertility
 [J]. Scientia Agricultura Sinica, 2009, 42(3): 809-815 .
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd