Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (20): 4321-4327.doi: 10.3864/j.issn.0578-1752.2013.20.015

• HORTICULTURE • Previous Articles     Next Articles

Effects of Phenanthrene and Pyrene on Ca2+, K+ and H+ Liquidity in Root Hair Cells of Malus hupehensis

 HUANG  Cui-Xiang, XIA  Yan-Fei, WANG  Rong, ZHANG  Wen-Hui, HAN  Tian-Tian, SHEN  Xiang   

  1. College of Horticultural Science and Engineering, Shandong Agricultural University，Taian 271018，Shandong
  • Received:2013-01-22 Online:2013-10-15 Published:2013-08-08

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Abstract: 【Objective】 The objective of this study is to prove PAHs to the plant growth mechanism, and the difference in substances poisoning effect of different PAHs. 【Method】 The Non-invasive Micro-test Technique was used to measure in Malus hupehensi sweet tea seedling root treated by using the polycyclic aromatic hydrocarbon phenanthrene and pyrene treated Ca2 +, K +and H +velocity variation. 【Result】 The Ca2 +, K + and H + liquidity dramatically reversed by contrasting from (-63.53±9.30） pmol•cm-2•s-1, （-60.56±14.56） pmol•cm-2•s-1, （+44.38±5.19 ）pmo•cm-2•s-1, changing to (+127.18±39.95) pmol•cm-2•s-1, (109.97±25.68) pmol•cm-2•s-1, and (-10.35±1.57 ) pmol•cm-2•s-1) treated with phenanthrene; and treated with pyrene, the Ca2+ , K +and H + liquidity was reversed by changing to （+220.29±60.42） pmo•cm-2•s-1,（140.21±27.87） pmol•cm-2•s-1, and（-14.42±3.16） pmol•cm-2•s-1. 【Conclusion】 PAHs can destroy the cell membrane permeability, reduce the ion channel activity and disturb the Ca2+ and K + ion absorption process of M. hupehensis, thus resulting in the lack of relevant elements, and pyrene can cause poisoning effect which is significantly higher than phenanthrene, and PAHs toxic to plants has become one of the mechanism. The conclusion was that the influence of polycyclic aromatic hydrocarbon on plant growth can be traced back to ion absorption and ion channel change for further study the influence of PAHs on plants and provide a theoretical basis of the influence.

Key words: polycyclic aromatic hydrocarbons(PAHs) , phenanthrene , pyrene , Malus hupehensis , Ca2+ , K+ , H+

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