Abstract: The effect of different color plastic films on the shoot regeneration and its mechanism from the leaf discs of Fragaria×ananassa Duch. cv. Toyonoka were studied in an experiment using an optimized MS medium (1.5 mg·L-1 TDZ and 0.4 mg·L-1 IBA). Color films had significant effects on shoot regeneration of strawberry. The highest percentage of shoot regeneration (> 95%) and maximum number of shoots per explant (>25) were observed under red and green films. Different spectrum ranges of the color films were 300 to 700 nm. Light intensity under red and green films was weaker than fluorescent light and yellow, blue films. Red and green films induced a higher level of chlorophyll and lower chlorophyll a/b in tissue culture of strawberry. Antioxidant enzyme (SOD, CAT and POD) activities were the highest under red films, followed by green and the lowest was under yellow ones. Antioxidant activities had positive correlation with the shoot regeneration while malondialdehyde (MDA) was negative. After 15 days in culture, GA3 content was higher under fluorescent light, red and green films, respectively. Zeatin (ZT) and gibberellic acid (GA3) contents were the highest under red and green films after 45 days, however, indole-3-acetic acid (IAA) accumulation in the tissues was higher under yellow and blue films.

Key words: Fragaria×ananassa Duch, Color films, Shoot regeneration, Antioxidants, Endogenous hormone