外源亚精胺对高温胁迫下生姜叶片内源激素及叶绿体超微结构的影响

doi:10.3864/j.issn.0578-1752.2015.01.12

Abstract

Abstract: 【Objective】Ginger is a thermophilic vegetable crop, but not tolerate high temperature. It is easily damaged under high temperature. To study the effect of exogenous spermidine on endogenous hormones and chloroplasts, the relationship among exogenous spermidine, endogenous hormones, and the chloroplast ultrastructure of ginger under heat stress was investigated.【Method】Laiwu Big Ginger was sandy cultured in a climate chamber with 12 h/12 h photoperiod under 28℃/18℃ (normal) and 38℃/28℃ (heat stress) conditions. Ginger root was treated with 0.5 mmol·L^-1spermidine. Relative water content, chlorophyll concentration, malondialdehyde content, electrolyte leakage, chlorophyll fluorescence parameters, reactive oxygen content and endogenous hormones metabolism of ginger leaves were investigated on 0, 5, 10, 15 and 20 d after treatment, moreover, the ultrastructures of chloroplasts and thylakoid were observed on 20 d after treatment. 【Result】Under heat stress, chloroplasts and thylakoid of ginger leaves were seriously damaged, and chlorophyll concentration significantly decreased with continuing stress. Chlorophyll fluorescence parameters including F_v/F_m, Ф_PS_II, F_v′/F_m′, q_P and P decreased, and NPQ, β/α-1 and D increased, which mainly showed that its photochemical activity of PSII was decreased, so the and H₂O₂ accumulated extensively resulted by excess energy, and then the MDA content and electrolyte leakage increased. Under heat stress, superoxide dismutase activity, abscisic acid and proline contents were significantly accumulated and then decreased in different treatment times. Ascorbate peroxidase activity, cytokinin and kinetin content kept a decreasing trend with continuing stress. However, relative water content, chlorophyll concentration, malondialdehyde content and electrolyte leakage of ginger leaves were recovered by exogenous application of spermidine. Besides, it also maintained the integrity of chloroplasts and thylakoid, regulated chlorophyll fluorescence parameters to normale, increased antioxidant enzymes activity and endogenous hormones concentration, and reduced the reactive oxygen content. 【Conclusion】Heat stress can damage ginger leaves, leading to function of chloroplasts and PSII disorder, and reactive oxygen and endogenous hormones abnormity. Exogenous application of spermidine can effectively reduce the damages of ginger caused by heat stress and improve its tolerance to heat stress, involving maintain endogenous hormones regular metabolism and chloroplasts normal physiological function.

Key words: ginger, heat stress, spermidine, chloroplasts, endogenous hormones

LI Xiu, GONG Biao, XU Kun. Effect of Exogenous Spermidine on Levels of Endogenous Hormones and Chloroplast Ultrastructure of Ginger Leaves Under Heat Stress[J].Scientia Agricultura Sinica, 2015, 48(1): 120-129.

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Effect of Exogenous Spermidine on Levels of Endogenous Hormones and Chloroplast Ultrastructure of Ginger Leaves Under Heat Stress

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