Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (23): 4929-4939.doi: 10.3864/j.issn.0578-1752.2020.23.017

• HORTICULTURE • Previous Articles     Next Articles

Regulation Mechanism of Carvone on Seed Potato Sprouting

GE Xia1(),XU Rui2,3,LI Mei1,TIAN JiaChun1,LI ShouQiang1,CHENG JianXin1,TIAN ShiLong1()   

  1. 1Institute of Agricultural Products Storage and Processing, Gansu Academy of Agricultural Sciences, Lanzhou 730070
    2Animal Husbandry, Pasture and Green Agriculture Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070
    3Institute of Agricultural Quality Standards and Testing Technology, Gansu Academy of Agricultural Sciences, Lanzhou 730070
  • Received:2020-04-08 Accepted:2020-09-04 Online:2020-12-01 Published:2020-12-09
  • Contact: ShiLong TIAN E-mail:12660940@qq.com;723619635@qq.com

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Abstract:

【Objective】 Carvone is a common monoterpene in plant essential oils. In this study, the partial regulation mechanism of carvone on seed potato sprouting was explored from the aspects of hormone regulation and membrane lipid peroxidation. 【Method】The cultivar Qingshu 9 was used as experimental material. The method of treatment was using 0.3 mL carvone per kilogram of tuber for 18 weeks, and then stopping chemical for 6 weeks before planting. At the same time, the treatment without using carvone was as control group. Sprout length, weight loss, abscisic acid (ABA), indoleacetic acid (IAA), gibberellic acid (GA3), malondialdehyde (MDA) and proline (PRO) contents, superoxide dismutase (SOD), catalase (CAT), polyphenol oxidase (PPO) and peroxidase (POD) enzyme activities were determined under different storage periods (at 0, 6, 18, 20 and 24 week). Meanwhile, the bud meristem of seed potato was sectioned and analyzed by microimaging regularly. 【Result】 Carvone treatment could regulate potato sprouting and the sprout length, and reduce the weight loss of seed potatoes. After 24 weeks of storage, the sprout length of the treated seed potatoes was 4.77 mm, significantly lower than that of the control group (51.02 mm), and the weight loss was reduced by 49.76%. Carvone treatment inhibited the growth of apical buds by damaging apical meristem and vascular tissue, which did not affect the emergence of axillary buds. During storage, carvone treatment significantly increased the ABA content, inhibited the IAA content, delayed the peak of GA3 content and the appearance of (GA3+IAA) /ABA >1 inflection point. For the carvone reated potatoes, MDA content in potato was 15.04%-25.90% lower than control group; PRO content was 24.59%-54.45% higher than control group; the activities of SOD, CAT and PPO were 18.86%-35.56%, 14.34%-31.97% and 26.00%-36.41% higher than control group, respectively; POD activity of treated potatoes was 1.87-3.07 times that of control group. 【Conclusion】 Carvone treatment adjusted the ABA, IAA and GA3 contents of seed potatoes by promoting/inhibiting 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) in the mevalonate pathway, and regulated the inhibition of the apical bud. In addition, it increased antioxidant enzymes activities, reduced the degree of membrane lipid peroxidation and alleviated seed potato aging.

Key words: carvone, regulation of potato sprouting, endogenous hormone, membrane lipid peroxidation, tissue slice

Fig. 1

The schematic of self-made storage box (65% refers to the percentage of the volume of mini tuber potatoes to the volume of storage box)"

Fig. 2

Effects of carvone treatment on sprout length (a) and weight loss (b) of seed potato during storage * indicate significant differences (P<0.05). The same as below"

Fig. 3

Effects of carvone treatment on seed potato apical meristem and sprouting a: Untreated meristem; b: Bud meristem treated with carvone 2 days after treatment; c: Bud meristem treated with carvone 7 days after treatment; d: Axillary bud began grow nearby necrotic apical meristem 3 days after carvone withdrawal; e: Axillary bud growth continues around the necrotic apical meristem 7 days after carvone withdrawal; f: Seed potatoes with carvone treatment stored for 20 weeks; g: Control seed potatoes stored for 24 weeks; h: Seed potatoes with carvone treatment stored for 24 weeks. Scale bar is 100 μm (a, b, c), 200 μm (d, e)"

Fig. 4

Effect of carvone treatment on the content of endogenous IAA (a), ABA (b) and GA3 (c), and ABA/(IAA+GA3) in seed potato during storage"

Fig. 5

Effects of carvone treatment on the content of MDA (a) and PRO (b) in seed potato during storage"

Fig. 6

Effects of carvone treatment on the activity of SOD (a), CAT (b), POD(c) and PPO(d) in seed potato during storage"

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