Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (23): 4929-4939.doi: 10.3864/j.issn.0578-1752.2020.23.017

• HORTICULTURE • Previous Articles     Next Articles

Regulation Mechanism of Carvone on Seed Potato Sprouting

GE Xia1(),XU Rui2,3,LI Mei1,TIAN JiaChun1,LI ShouQiang1,CHENG JianXin1,TIAN ShiLong1()   

  1. 1Institute of Agricultural Products Storage and Processing, Gansu Academy of Agricultural Sciences, Lanzhou 730070
    2Animal Husbandry, Pasture and Green Agriculture Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070
    3Institute of Agricultural Quality Standards and Testing Technology, Gansu Academy of Agricultural Sciences, Lanzhou 730070
  • Received:2020-04-08 Accepted:2020-09-04 Online:2020-12-01 Published:2020-12-09
  • Contact: ShiLong TIAN E-mail:12660940@qq.com;723619635@qq.com

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Abstract:

【Objective】 Carvone is a common monoterpene in plant essential oils. In this study, the partial regulation mechanism of carvone on seed potato sprouting was explored from the aspects of hormone regulation and membrane lipid peroxidation. 【Method】The cultivar Qingshu 9 was used as experimental material. The method of treatment was using 0.3 mL carvone per kilogram of tuber for 18 weeks, and then stopping chemical for 6 weeks before planting. At the same time, the treatment without using carvone was as control group. Sprout length, weight loss, abscisic acid (ABA), indoleacetic acid (IAA), gibberellic acid (GA3), malondialdehyde (MDA) and proline (PRO) contents, superoxide dismutase (SOD), catalase (CAT), polyphenol oxidase (PPO) and peroxidase (POD) enzyme activities were determined under different storage periods (at 0, 6, 18, 20 and 24 week). Meanwhile, the bud meristem of seed potato was sectioned and analyzed by microimaging regularly. 【Result】 Carvone treatment could regulate potato sprouting and the sprout length, and reduce the weight loss of seed potatoes. After 24 weeks of storage, the sprout length of the treated seed potatoes was 4.77 mm, significantly lower than that of the control group (51.02 mm), and the weight loss was reduced by 49.76%. Carvone treatment inhibited the growth of apical buds by damaging apical meristem and vascular tissue, which did not affect the emergence of axillary buds. During storage, carvone treatment significantly increased the ABA content, inhibited the IAA content, delayed the peak of GA3 content and the appearance of (GA3+IAA) /ABA >1 inflection point. For the carvone reated potatoes, MDA content in potato was 15.04%-25.90% lower than control group; PRO content was 24.59%-54.45% higher than control group; the activities of SOD, CAT and PPO were 18.86%-35.56%, 14.34%-31.97% and 26.00%-36.41% higher than control group, respectively; POD activity of treated potatoes was 1.87-3.07 times that of control group. 【Conclusion】 Carvone treatment adjusted the ABA, IAA and GA3 contents of seed potatoes by promoting/inhibiting 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) in the mevalonate pathway, and regulated the inhibition of the apical bud. In addition, it increased antioxidant enzymes activities, reduced the degree of membrane lipid peroxidation and alleviated seed potato aging.

Key words: carvone, regulation of potato sprouting, endogenous hormone, membrane lipid peroxidation, tissue slice

Fig. 1

The schematic of self-made storage box (65% refers to the percentage of the volume of mini tuber potatoes to the volume of storage box)"

Fig. 2

Effects of carvone treatment on sprout length (a) and weight loss (b) of seed potato during storage * indicate significant differences (P<0.05). The same as below"

Fig. 3

Effects of carvone treatment on seed potato apical meristem and sprouting a: Untreated meristem; b: Bud meristem treated with carvone 2 days after treatment; c: Bud meristem treated with carvone 7 days after treatment; d: Axillary bud began grow nearby necrotic apical meristem 3 days after carvone withdrawal; e: Axillary bud growth continues around the necrotic apical meristem 7 days after carvone withdrawal; f: Seed potatoes with carvone treatment stored for 20 weeks; g: Control seed potatoes stored for 24 weeks; h: Seed potatoes with carvone treatment stored for 24 weeks. Scale bar is 100 μm (a, b, c), 200 μm (d, e)"

Fig. 4

Effect of carvone treatment on the content of endogenous IAA (a), ABA (b) and GA3 (c), and ABA/(IAA+GA3) in seed potato during storage"

Fig. 5

Effects of carvone treatment on the content of MDA (a) and PRO (b) in seed potato during storage"

Fig. 6

Effects of carvone treatment on the activity of SOD (a), CAT (b), POD(c) and PPO(d) in seed potato during storage"

[1] SARAIVA J A, RODRIGUES I M . Inhibition of potato tuber sprouting by pressure treatments. International Journal of Food Science and Technology, 2011,46(1):61-66.
doi: 10.1111/j.1365-2621.2010.02455.x
[2] CAMPBELL M, SEGEAR E, BEERS L, KNAUBER D, SUTTLE J C . Dormancy in potato tuber meristems: Chemically induced cessation in dormancy matches the natural process based on transcript profiles. Functional and Integrative Genomics, 2008,8(4):317-328.
doi: 10.1007/s10142-008-0079-6 pmid: 18317824
[3] SONNEWALD U . Control of potato tuber sprouting. Trends in Plant Science, 2001,6(8):333-335.
doi: 10.1016/s1360-1385(01)02020-9 pmid: 11495763
[4] TEPER-BAMNOLKER P, DUDAI N, FISCHER R, BELAUSOV E, ZEMACH H, SHOSEYOV O, ESHEL D . Mint essential oil can induce or inhibit potato sprouting by differential alteration of apical meristem. Planta, 2010,232(1):179-186.
doi: 10.1007/s00425-010-1154-5
[5] SORCE C, LORENZI R, PARISI B, RANALLI P . Physiological mechanisms involved in potato (Solanum tuberosum) tuber dormancy and the control of sprouting by chemical suppressants. Acta Horticulturae, 2005,684(1):177-185.
[6] 黄涛, 叶旭, 黄雪丽, 李昕昀, 梅猛, 余丽萍, 邹雪, 王西瑶 . 薄荷醇和茉莉精油对马铃薯抑芽效果研究. 四川农业大学学报, 2018,36(5):618-625.
HUANG T, YE X, HUANG X L, LI X Y, MEI M, YU L P, ZOU X, WANG X Y . Study of menthol and jasmine oil on potato sprout inhibition. Journal of Sichuan Agricultural University, 2018,36(5):618-625. (in Chinese)
[7] 普红梅, 杨万林, 刘凌云, 杨琼芬, 白建明, 李燕山, 姚春光 . 薄荷精油对‘云薯304’原原种抑芽效果研究. 作物研究, 2018,32(3):194-197.
PU H M, YANG W L, LIU L Y, YANG Q F, BAI J M, LI Y S, YAO C G . Study on the effect of mint essential oil on the germination of ‘Yunshu 304’ breeder's seed. Crop Research, 2018,32(3):194-197. (in Chinese).
[8] SHUKLA S, PANDEY S S, CHANDRA M, PANDEY A, BHARTI N, BARNAWAL D, CHANOTIYA S C, TANDON S, DAROKAR M P, KALRA A . Application of essential oils as a natural and alternate method for inhibiting and inducing the sprouting of potato tubers. Food Chemistry, 2019,284:171-179.
doi: 10.1016/j.foodchem.2019.01.079 pmid: 30744843
[9] BAYDAR H, KARADOGAN T . The effects of volatile oils on in vitro potato sprout growth. Potato research, 2003,46(1/2):1-8.
doi: 10.1007/BF02736098
[10] SHARMA A . Essential oil as organic and alternative methods for potato. International Journal of Engineering and Mathematical Sciences, 2012,1:34-39.
[11] ŞANLI A KARADOĞAN T . Carvone containing essential oils as sprout suppressants in potato (Solanum tuberosum L.) tubers at different storage temperatures. Potato Research, 2019,62:345-360.
doi: 10.1007/s11540-019-9415-6
[12] HARTMANS K J, DIEPENHORST P, BAKKER W, GORRIS L G . The use of carvone in agriculture: Sprout suppression of potatoes and antifungal activity against potato tuber and other plant diseases. Industrial Crops and Products, 1995,4(1):3-13.
doi: 10.1016/0926-6690(95)00005-W
[13] OOSTERHAVEN K, HARTMANS K J, SCHEFFER J J C . Inhibition of potato sprout growth by carvone enantiomers and their bioconversion in sprouts. Potato Research, 1995,38:219-230.
doi: 10.1007/BF02357935
[14] 葛霞, 田世龙, 田甲春, 李梅, 李守强, 程建新 . 香芹酮处理对马铃薯微型薯发芽调控及田间种植的影响. 中国马铃薯, 2019,33(3):175-183.
GE X, TIAN S L, TIAN J C, LI M, LI S Q, CHENG J X . Effects of carvone on minituber sprout regulation and field planting. Chinese Potato Journal, 2019,33(3):175-183. (in Chinese)
[15] OOSTERHAVEN K, HARTMANS K J, HUIZING H J . Inhibition of potato (Solanum tuberosum) sprout growth by the monoterpene carvone: reduction of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity without effect on its mRNA level. Journal of Plant Physiology, 1993,141:463-469.
doi: 10.1016/S0176-1617(11)80195-1
[16] 钟蕾, 邓俊才, 王良俊, 郑顺林, 豆攀, 王兴龙, 袁继超 . 生长调节剂处理对马铃薯贮藏期萌发及氧化酶活性的影响. 草业学报, 2017,26(7):147-157.
ZHONG L, DENG J C, WANG L J, ZHENG S L, DOU P, WANG X L, YUAN J C . Effect of plant growth regulators on germination and oxidase activity during storage of potato tubers. Acta Prataculturae Sinica, 2017,26(7):147-157. (in Chinese)
[17] KAMENETSKY R . Life cycle, flower initiation, and propagation of the desert geophytes Allium rothii. International Journal of Plant Sciences, 1994,155:597-605.
doi: 10.1086/297198
[18] 高俊凤 . 植物生理学实验指导. 北京: 高等教育出版社, 2006.
GAO J F. Experimental Guidance of Plant Physiology. Beijing: High Education Press, 2006. (in Chinese)
[19] 曹健康, 姜微波, 赵玉梅 . 果蔬采后生理生化实验指导. 北京: 中国轻工业出版社, 2007.
CAO J K, JIANG W B, ZHAO Y M. Experiment Guidance of Postharvest Physiology and Biochemistry of Fruits and Vegetables. Beijing: China Light Industry Press, 2007. (in Chinese)
[20] 李合生 . 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000.
LI H S. Principle and Technology of Plant Physiological Biochemical Experiment. Beijing: Higher Education Press, 2000. (in Chinese)
[21] AFIFY A, EL-BELTAGI H, ALY A, EL-ANSARY A A . Antioxidant enzyme activities and lipid peroxidation as biomarkercompounds for potato tuber stored by gamma radiation. Asian Pacific Journal of Tropical Biomedicine, 2012,12:1548-1555.
[22] 何献君, 吕光璞, 陈雅惠 . 过氧化物酶酶促动力学实验的设计. 实验技术与管理, 2009,26(6):33-35.
HE X J, LÜ G P, CHEN Y H . Design for dynamics experiment of peroxidase. Experimental Technology and Management, 2009,26(6):33-35. (in Chinese)
[23] GOLDSTEIN J L, BROWN M S . Regulation of the mevalonate pathway. Nature, 1990,343:425-430.
doi: 10.1038/343425a0 pmid: 1967820
[24] BACH T J, BORONAT A, CAMPOS N, FERRER A, VOLLACK K . Mevalonate biosynthesis in plants. Critical Reviews in Biochemistry and Molecular Biology, 1999,34(2):107-122.
doi: 10.1080/10409239991209237 pmid: 10333388
[25] VIOLA R, PELLOUX J, VAN DER PLOEG A V, GILLESPIE T, MARQUIS N, ROBERTS A G, HANCOCK R D . Symplastic connection is required for bud outgrowth following dormancy in potato (Solanum tuberosum L.) tubers. Plant, Cell and Environment, 2007,30(8):973-983.
doi: 10.1111/j.1365-3040.2007.01692.x pmid: 17617825
[26] SCHOPFER P, PLACHY C . Control of seed germination by abscisic acid: iii. Effect on embryo growth potential (minimum turgor pressure) and growth coefficient (cell wall extensibility) in Brassica napus L. Plant Physiology, 1985,77(3):676-686.
doi: 10.1104/pp.77.3.676 pmid: 16664118
[27] CLELAND R E . Auxin and Cell Elongation//DAVIES P J. Plant Hormones: Physiology, Biochemistry and Molecular Biology. 2nd ed. Netherland: Kluwer Academic Publisher, 1995: 214-227.
[28] WILTSHIRE J J J, COBB A H . A review of the physiology of potato tuber dormancy. Annals of Applied Biology, 1996,129(3):553-569.
doi: 10.1111/aab.1996.129.issue-3
[29] SUTTLE J C . Physiological regulation of potato tuber dormancy. American Journal of Potato Research, 2004,81(4):253-262.
doi: 10.1007/BF02871767
[30] JEEVAN KUMAR S P, PRASAD S R, BANERJEE R, THAMMINENI C . Seed birth to death: Dual functions of reactive oxygen species in seed physiology. Annals of Botany, 2015,116(4):663-668.
doi: 10.1093/aob/mcv098 pmid: 26271119
[31] GOMES M P, GARCIA Q S . Reactive oxygen species and seed germination. Biologia, 2013,68(3):351-357.
[32] LIU Y G, YE N H, LIU R, CHEN M, ZHANG J . H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination. Journal of Experimental Botany, 2010,61(11):2979-2990.
doi: 10.1093/jxb/erq125 pmid: 20460363
[33] ISHIBASHI Y, KASA S, SAKAMOTO M, AOKI N, KAI K, YUASA T, HANADA A, YAMAGUCHI S, IWAYAINOUE M . A role for reactive oxygen species produced by NADPH oxidases in the embryo and aleurone cells in barley seed germination. PLoS ONE, 2015,10(11):1-17.
[34] HELTOFT P, WOLD A B, MOLTEBERG E L . Effect of ventilation strategy on storage quality indicators of processing potatoes with different maturity levels at harvest. Postharvest Biology and Technology, 2016,117:21-29.
doi: 10.1016/j.postharvbio.2016.02.002
[35] 叶旭 . 3种植物精油对马铃薯贮藏保鲜效应的研究[D]. 成都: 四川农业大学, 2015.
YE X . Research on potatoes storage effect of three plant essential oils[D]. Chengdu: Sichuan Agricultural University, 2015. (in Chinese).
[36] COLEMAN W K, KING R R . Changes in endogenous abscisic acid, soluble sugars and proline levels during tuber dormancy in Solanum tuberosum L. American Potato Journal, 1984,61(8):437.
doi: 10.1007/BF02852813
[37] 王鹏, 连勇, 金黎平 . 马铃薯块茎休眠及萌发过程中几种酶活性变化. 华北农学报, 2003,18(1):33-36.
doi: 10.3321/j.issn:1000-7091.2003.01.010
WANG P, LIAN Y, JIN L P . The research on the regulation of enzymes during dormancy and dormancy releasing. Acta Agriculturae Boreali-Sinica, 2003,18(1):33-36. (in Chinese)
doi: 10.3321/j.issn:1000-7091.2003.01.010
[38] 杨晓玲, 张建文, 刘永军, 郭守华, 刘贵荣, 程秀娟 . 马铃薯块茎发芽过程中酚类物质含量及其相关酶活性的变化. 植物生理学通讯, 2002,38(4):347-348.
YANG X L, ZHANG J W, LIU Y J, GUO S H, LIU G R, CHENG X J . Changes in phenols content and activities of relative enzymes during germination of potato tuber. Plant Physiology Communications, 2002,38(4):347-348. (in Chinese)
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