Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (03): 571-575 .doi: 10.3864/j.issn.0578-1752.at-2004-2443

• HORTICULTURE • Previous Articles     Next Articles

Cloning of cDNA Fragment of L-Galactono-1,4-Lactone Dehydrogenase and Its Expression in Different Organs of Rosa roxburghii Tratt

  

  1. 浙江大学园艺系
  • Received:2004-06-03 Revised:2004-09-27 Online:2005-03-10 Published:2005-03-10

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Abstract: 855 bp cDNA encoding L-galactono-1,4-lactone dehydrogenase (GalLDH) fragment was cloned from fruit of Rosa. roxburghii Tratt by the method of RT-PCR on the basis of the homologous genes of Arabidopsis thaliana, cauliflower, sweet potato, strawberry, etc. in GenBank. Sequensing analysis shows that 79%-92% identity in nucleotide sequence and 75%-87% identity in amino acid sequence to that of strawberry and Arabidopsis thaliana, etc. was observed. Northern blot showed that the expression of GalLDH was significantly different in different organs. The transcription level of GalLDH in fruit was significantly higher than that in leaf, stem and root, respectively. Furthermore, this expression mode was highly correlated with AsA levels.

Key words: Rosa roxburghii Tratt, L-galactono-1, 4-lactone dehydrogenase (GalLDH), Ascorbic acid (AsA), Cloning, Gene expression

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