Scientia Agricultura Sinica ›› 2004, Vol. 37 ›› Issue (09): 1369- .doi: 10.3864/j.issn.0578-1752.040919

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Homogenous Identification of a Group of Polymorphic High Molecular Weight Protein in Sow Milk

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  1. 南京农业大学农业部动物生理生化重点开放实验室
  • Received:2003-11-20 Revised:2004-06-16 Online:2004-09-20 Published:2004-09-20

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Abstract: High molecular weight proteins (HMWP) in sow milk showed four variants of A, B, C, and D by SDS-PAGE. HMWP-B was purified by a combination of ammonium precipitation, SephadexG-200 gel filtration chromatography and High Q column anion-exchange chromatography. The rabbit anti- HMWP-B Polyclonal Antibody was then raised. Immunoblotting of HMWP in sow milk showed immunological cross reaction among these variants and indicated HMWP in sow milk are homologous. The result of this study provides a proof for the hypothesis of polymorphism of HMWP .

Key words: Sow milk, High molecular weight protein (HMWP), Polymorphism, Homology

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