Scientia Agricultura Sinica ›› 2023, Vol. 56 ›› Issue (15): 3020-3031.doi: 10.3864/j.issn.0578-1752.2023.15.015

• ANIMAL SCIENCE·VETERINARY SCIENCE • Previous Articles     Next Articles

Screening of Anti-Apoptotic Protein GRP94 Interaction Proteins in Porcine Hepatic Stellate Cells by Immunoprecipitation Combined with LC-MS/MS

WANG XiaoHong(), XING MingJie, GU XianHong, HAO Yue()   

  1. Institute of Animal Science, Chinese Academy of Agricultural Sciences/State Key Laboratory of Animal Nutrition and Feeding, Beijing 100193
  • Received:2022-05-10 Accepted:2022-07-14 Online:2023-08-01 Published:2023-08-05

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Abstract:

【Background】 Extensive stress reactions often occur in pigs due to poor breeding environment, thick subcutaneous fat and lack of sweat glands in pigs. Endoplasmic reticulum (ER) stress (ERS) may be the earliest stress response, which is closely related to apoptosis and metabolism. Liver is the main metabolic organ of the body. In the process of pig breeding, the artificial operations, such as weaning, feed mildew, and inhalation of harmful gases and temperature changes, will cause ERS in pig liver, which will not only cause liver damage, but also cause liver fat metabolism disorder and extensive inflammatory reaction, affecting animal production performance and reproductive performance. The regulation of ERS in production is helpful to reduce the recessive loss in the pig breeding process. 【Objective】 Immunoprecipitation combined with mass spectrometry was used to screen the cellular proteins interacting with glucose-regulated protein 94 (GRP94) in porcine hepatic stellate cells (HSC) under ERS condition, which could lay a foundation for further study on the protective mechanism of GRP94 on biological function of HSC. 【Method】 Protein complexes bound to GRP94 bait protein were eluted, collected, and verified by SDS-PAGE gel electrophoresis. The successfully verified sample eluent was detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the interaction proteins of GRP94 under normal condition and ERS condition were identified. The detected proteins were analyzed by GO, KEGG and interaction network. Vimentin, one of the interacting proteins, was verified by co-immunoprecipitation. 【Result】 In porcine hepatic stellate cells, 146 proteins were interacting with GRP94 under normal condition, 76 proteins under ERS condition, and 44 proteins under both conditions. The results showed that 76 proteins interacting with GRP94 under ERS were mainly involved in the negative regulation of apoptosis process, peptide cross-linking, ubiquitin-dependent ERAD (endoplasmic reticulum associated degradation) pathway and hydrogen peroxide catabolic process, among them, the specific proteins interacting with GRP94 in the negative regulation of apoptosis process were albumin, catalase, filament A, heat shock protein family A member 5, keratin 18, and prohibin 2, indicating that GRP94 might play an anti-apoptotic role with these proteins. Besides, the vimentin protein that made up the intermediate filament was involved in multiple GO enrichment terms, which might have an important interaction with GRP94, which was further confirmed by co-immunoprecipitation test, further demonstrating that there was indeed an interactive relationship between this two. Further analysis showed that some GRP94 interacting proteins (Such as peroxiredoxin, death inducer obliterator 1, catalase, glandular kallikrein, pyruvate kinase and so on) specifically expressed under ERS were closely related to anti-apoptosis. 【Conclusion】 Under ERS, GRP94 interacting proteins were mainly involved in anti-apoptosis, folding of unfolded proteins and maintenance of intracellular homeostasis-related signal pathways. This conclusion laid a foundation for further study on the mechanism of GRP94 involved in liver ERS regulation.

Key words: endoplasmic reticulum stress, liver cells, GRP94, protein interactions, pig

Fig. 1

WB result of precipitation of GRP94 WB verified the success of immunoprecipitation of GRP94-associated proteins under normal condition and ERS condition; Input: cell lysates; input+TM: cell lysates treated with TM; IP: Immunoprecipitation samples prepared after extraction of cellular proteins and incubation of GRP94 antibodies; IP+TM: Immunoprecipitation samples prepared after extraction of cellular proteins treated with TM and incubation of GRP94 antibodies; IgG: IP with control mouse IgG; IgG+TM: IP treated with TM with control mouse IgG"

Fig. 2

Venn plot of GRP94 interacting proteins IP corresponded to the blue circle, indicating that 146 proteins interacted with GRP94 under normal condition, and TM_IP corresponded to the red circle, indicating that 76 proteins interacted with GRP94 under ERS. The overlapping area of the two circles was dark red, indicating that 44 proteins interacted with GRP94 under both conditions"

Fig. 3

GO analysis of GRP94 interacting proteins A-C: GO functional enrichment analysis of GRP94 interacting proteins (Biological process, Cellular component and Molecular function). The ordinate was the GO item, and the abscissa represented the enrichment of protein in the corresponding function item. The larger the value of -log(P value) was, the more relevant the protein was to the function"

Fig. 4

KEGG analysis of GRP94 interacting proteins Each dot in the figure represented a KEGG pathway. The size of the dot represented the number of proteins enriched into this pathway, and the color of the dot represented P value. The smaller P value was, the more reliable the protein enrichment significance in this pathway was. The vertical axis represented the pathway name, and the horizontal axis represented -lg(P value)"

Fig. 5

Interaction analysis of GRP94 binding proteins In this experiment, protein ID was compared with protein in STRING database, and the Confidence score was greater than 0.4 to obtain protein interaction information. Functional interaction network model was integrated using STRING tool, a node in the graph presented a protein, the line between two nodes meant the existence of interaction in this two proteins, and the thickness of the line reflected the strength of the interaction"

Fig. 6

Co-immunoprecipitation analysis of GRP94 and vimentin Co-immunoprecipitation verified the interaction of vimentin and GRP94; input: cell lysates; IP: Immunoprecipitation samples prepared after extraction of cellular proteins and incubation of GRP94 antibodies; IgG: IP with control mouse IgG"

[1]
甘靖宇, 孙维婧, 卢嘉茵, 王子旭, 陈耀星, 董玉兰. 限位栏对孕猪应激水平及神经内分泌HPA轴的影响. 中国兽医杂志, 2021, 57(7): 1-6.
GAN J Y, SUN W J, LU J Y, WANG Z X, CHEN Y X, DONG Y L. Effect of restraint stress on stress level of pregnant sows and neuroendocrine HPA axis. Chinese Journal of Veterinary Medicine, 2021, 57(7): 1-6. (in Chinese)
[2]
YONG Y H, LI J Y, GONG D L, YU T Y, WU L Y, HU C Y, LIU X X, YU Z C, MA X B, GOONERATNE R, EL-ATY A M A, CHEN J J, JU X H. ERK1/2 mitogen-activated protein kinase mediates downregulation of intestinal tight junction proteins in heat stress-induced IBD model in pig. Journal of Thermal Biology, 2021, 101: 103103.

doi: 10.1016/j.jtherbio.2021.103103
[3]
LIAO J Z, HU Z Y, LI Q W, LI H J, CHEN W J, HUO H H, HAN Q Y, ZHANG H, GUO J Y, HU L M, PAN J Q, LI Y, TANG Z X.Endoplasmic Reticulum stress contributes to copper-induced pyroptosis via regulating the IRE1α-XBP1 pathway in pig jejunal epithelial cells. Journal of Agricultural and Food Chemistry, 2022, (4): 1293-1303.
[4]
KAPOOR A, MATTHEWS S G. Prenatal stress modifies behavior and hypothalamic-pituitary-adrenal function in female Guinea pig offspring: Effects of timing of prenatal stress and stage of reproductive cycle. Endocrinology, 2008, 149(12): 6406-6415.

doi: 10.1210/en.2008-0347 pmid: 18755800
[5]
WU J, KAUFMAN R J. From acute ER stress to physiological roles of the Unfolded Protein Response. Cell Death & Differentiation, 2006, 13(3): 374-384.
[6]
CORAZZARI M, GAGLIARDI M, FIMIA G M, PIACENTINI M. Endoplasmic Reticulum stress, unfolded protein response, and cancer cell fate. Frontiers in Oncology, 2017, 7: 78.

doi: 10.3389/fonc.2017.00078
[7]
ZHU G Y, LEE A S. Role of the unfolded protein response, GRP78 and GRP94 in organ homeostasis. Journal of Cellular Physiology, 2015, 230(7): 1413-1420.

doi: 10.1002/jcp.24923 pmid: 25546813
[8]
CUI Y, HAO Y, LI J, GAO Y, GU X. Proteomic changes of the porcine skeletal muscle in response to chronic heat stress. Journal of the Science of Food and Agriculture, 2018, 98(9): 3315-3323.

doi: 10.1002/jsfa.8835 pmid: 29239490
[9]
HE Y, FAN X X, LIU N, SONG Q Q, KOU J, SHI Y H, LUO X, DAI Z L, YANG Y, WU Z L, WU G Y. L-glutamine represses the unfolded protein response in the small intestine of weanling piglets. The Journal of Nutrition, 2019, 149(11): 1904-1910.

doi: 10.1093/jn/nxz155
[10]
YOGALAKSHMI B, SATHIYA PRIYA C, ANURADHA C V. Grape seed proanthocyanidins and metformin combination attenuate hepatic endoplasmic reticulum stress in rats subjected to nutrition excess. Archives of Physiology and Biochemistry, 2019, 125(2): 174-183.

doi: 10.1080/13813455.2018.1444058 pmid: 29482356
[11]
LONG M, CHEN X L, WANG N, WANG M Y, PAN J W, TONG J J, LI P, YANG S H, HE J B. Proanthocyanidins protect epithelial cells from Zearalenone-induced apoptosis via inhibition of endoplasmic Reticulum stress-induced apoptosis pathways in mouse small intestines. Molecules (Basel, Switzerland), 2018, 23(7): 1508.

doi: 10.3390/molecules23071508
[12]
XU X G, ZHANG H L, ZHANG Q, HUANG Y, DONG J, LIANG Y B, LIU H J, TONG D W. Porcine epidemic diarrhea virus N protein prolongs S-phase cell cycle, induces endoplasmic reticulum stress, and up-regulates interleukin-8 expression. Veterinary Microbiology, 2013, 164(3/4): 212-221.

doi: 10.1016/j.vetmic.2013.01.034
[13]
文伟, 陈凤鸣, 黄兴国, 李颖慧. 肠上皮细胞中内质网应激与动物肠道炎症机制的研究进展. 动物营养学报, 2020, 32(2): 530-539.

doi: 10.3969/j.issn.1006-267x.2020.02.007
WEN W, CHEN F M, HUANG X G, LI Y H. Research progress of endoplasmic Reticulum stress and intestinal inflammation mechanism in intestinal epithelial cells. Chinese Journal of Animal Nutrition, 2020, 32(2): 530-539. (in Chinese)
[14]
PU J N, TIAN G, LI B, CHEN D W, HE J, ZHENG P, MAO X B, YU J, HUANG Z Q, YU B. Trace mineral overload induced hepatic oxidative damage and apoptosis in pigs with long-term high-level dietary mineral exposure. Journal of Agricultural and Food Chemistry, 2016, 64(8): 1841-1849.

doi: 10.1021/acs.jafc.5b05613 pmid: 26829127
[15]
LI S J, DING S T, MERSMANN H J, CHU C H, HSU C D, CHEN C Y. A nutritional nonalcoholic steatohepatitis minipig model. The Journal of Nutritional Biochemistry, 2016, 28: 51-60.

doi: 10.1016/j.jnutbio.2015.09.029
[16]
WANG X H, XIN H R, XING M J, GU X H, HAO Y. Acute endoplasmic Reticulum stress induces inflammation reaction, complement system activation, and lipid metabolism disorder of piglet livers: A proteomic approach. Frontiers in Physiology, 2022, 13: 857853.

doi: 10.3389/fphys.2022.857853
[17]
WANG X, WANG S X, LIU Y T, DING W C, ZHENG K, XIANG Y F, LIU K S, WANG D M, ZENG Y Y, XIA M, YANG D P, WANG Y F. The Hsp90 inhibitor SNX-2112 induces apoptosis of human hepatocellular carcinoma cells: The role of ER stress. Biochemical and Biophysical Research Communications, 2014, 446(1): 160-166.

doi: 10.1016/j.bbrc.2014.02.081 pmid: 24582562
[18]
LU T, WANG Y, XU K, ZHOU Z J, GONG J, ZHANG Y G, GONG H, DAI Q, YANG J, XIONG B, SONG Z, YANG G. Co-downregulation of GRP78 and GRP94 induces apoptosis and inhibits migration in prostate cancer cells. Open Life Sciences, 2019, 14: 384-391.

doi: 10.1515/biol-2019-0043
[19]
MORITO D, NAGATA K. ER stress proteins in autoimmune and inflammatory diseases. Frontiers in Immunology, 2012, 3: 48.

doi: 10.3389/fimmu.2012.00048 pmid: 22566930
[20]
GARDNER B M, PINCUS D, GOTTHARDT K, GALLAGHER C M, WALTER P. Endoplasmic reticulum stress sensing in the unfolded protein response. Cold Spring Harbor Perspectives in Biology, 2013, 5(3): a013169.
[21]
BARTON E R, PARK S, JAMES J K, MAKAREWICH C A, PHILIPPOU A, ELETTO D, LEI H, BRISSON B, OSTROVSKY O, LI Z, ARGON Y. Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production. FASEB Journal, 2012, 26(9): 3691-3702.

doi: 10.1096/fj.11-203026 pmid: 22649033
[22]
WENDRICH J R, BOEREN S, MÖLLER B K, WEIJERS D, DE RYBEL B, In vivo identification of plant protein complexes using IP-MS/MS. Methods in Molecular Biology (Clifton, N J), 2017, 1497: 147-158.
[23]
王加峰, 刘浩, 王慧, 陈志强. 水稻NBS-LRR类抗稻瘟病蛋白Pik-h的互作蛋白筛选. 中国农业科学, 2016, 49(3): 482-490.

doi: 10.3864/j.issn.0578-1752.2016.03.007
WANG J F, LIU H, WANG H, CHEN Z Q. Screening of putative proteins that are interacted with NBS-LRR protein Pik-h by the yeast two-hybrid system. Scientia Agricultura Sinica, 2016, 49(3): 482-490. (in Chinese)
[24]
窦万福, 祁静静, 胡安华, 陈善春, 彭爱红, 许兰珍, 雷天刚, 姚利晓, 何永睿, 李强. GST pull-down联合LC-MS/MS筛选柑橘抗溃疡病转录因子CsBZIP40的互作蛋白. 中国农业科学, 2019, 52(13): 2243-2255.

doi: 10.3864/j.issn.0578-1752.2019.13.005
DOU W F, QI J J, HU A H, CHEN S C, PENG A H, XU L Z, LEI T G, YAO L X, HE Y R, LI Q. Screening of interacting proteins of anti-canker transcription factor CsBZIP40 in Citrus by GST pull-down combined with LC-MS/MS. Scientia Agricultura Sinica, 2019, 52(13): 2243-2255. (in Chinese)
[25]
原贵波, 莫双榕, 钱莹, 臧栋楠, 杨帆, 蒋红亮, 武媛, 丁海东. 应用GST pull-down技术筛选番茄SIVQ6互作蛋白. 中国农业科学, 2020, 53(15): 3146-3157.

doi: 10.3864/j.issn.0578-1752.2020.15.014
YUAN G B, MO S R, QIAN Y, ZANG D N, YANG F, JIANG H L, WU Y, DING H D. Screening of interacting protein of tomato SIVQ6 by GST pull-down. Scientia Agricultura Sinica, 2020, 53(15): 3146-3157. (in Chinese)

doi: 10.3864/j.issn.0578-1752.2020.15.014
[26]
辛海瑞, 张闯, 郝月, 顾宪红. 猪肝星状细胞内质网应激模型的建立及对泛素化的影响. 中国畜牧兽医, 2020, 47(2): 399-406.
XIN H R, ZHANG C, HAO Y, GU X H. Establishment of endoplasmic Reticulum stress model of porcine hepatic stellate cell and its effect on ubiquitination. China Animal Husbandry ＆ Veterinary Medicine, 2020, 47(2): 399-406. (in Chinese)
[27]
辛海瑞. 应激条件下GRP94调节猪肝脏细胞损伤的机理[D]. 北京: 中国农业科学院, 2019.
XIN H R. Regulatory mechanism of GRP94 on liver cell damage of pig under stress condition[D]. Beijing: Chinese Academy of Agricultural Sciences, 2019. (in Chinese)
[28]
边晨晨. 内质网应激对草鱼脂肪细胞脂质蓄积的影响及机制研究[D]. 杨凌: 西北农林科技大学, 2020.
BIAN C C. The effect of endoplasmicreticulum stress on lipogenesis andthe molecular mechanism in grasscarp(Ctenopharyngodon idellus)[D]. Yangling: Northwest A & F University, 2020. (in Chinese)
[29]
DENG S X, LIU S P, JIN P, FENG S J, TIAN M, WEI P J, ZHU H D, TAN J Y, ZHAO F, GONG Y. Albumin reduces oxidative stress and neuronal apoptosis via the ERK/Nrf2/HO-1 pathway after intracerebral hemorrhage in rats. Oxidative Medicine and Cellular Longevity, 2021, 2021: 8891373.
[30]
GONÇALVES G L, COSTA-PESSOA J M, THIEME K, LINS B B, OLIVEIRA-SOUZA M. Intracellular albumin overload elicits endoplasmic reticulum stress and PKC-delta/p38 MAPK pathway activation to induce podocyte apoptosis. Scientific Reports, 2018, 8(1): 18012.

doi: 10.1038/s41598-018-36933-9 pmid: 30573754
[31]
SHIN S K, CHO H W, SONG S E, BAE J H, IM S S, HWANG I, HA H, SONG D K. Ablation of catalase promotes non-alcoholic fatty liver via oxidative stress and mitochondrial dysfunction in diet-induced obese mice. Pflügers Archiv - European Journal of Physiology, 2019, 471(6): 829-843.

doi: 10.1007/s00424-018-02250-3
[32]
HWANG I, UDDIN M J, PAK E S, KANG H, JIN E J, JO S, KANG D M, LEE H, HA H. The impaired redox balance in peroxisomes of catalase knockout mice accelerates nonalcoholic fatty liver disease through endoplasmic reticulum stress. Free Radical Biology and Medicine, 2020, 148: 22-32.

doi: S0891-5849(19)30660-4 pmid: 31877356
[33]
POPOWICZ G M, SCHLEICHER M, NOEGEL A A, HOLAK T A. Filamins: Promiscuous organizers of the cytoskeleton. Trends in Biochemical Sciences, 2006, 31(7): 411-419.

pmid: 16781869
[34]
VAN VLIET A R, GIORDANO F, GERLO S, SEGURA I, VAN EYGEN S, MOLENBERGHS G, ROCHA S, HOUCINE A, DERUA R, VERFAILLIE T, VANGINDERTAEL J, DE KEERSMAECKER H, WAELKENS E, TAVERNIER J, HOFKENS J, ANNAERT W, CARMELIET P, SAMALI A, AGOSTINIS P. The ER stress sensor PERK coordinates ER-plasma membrane contact site formation through interaction with filamin-A and F-actin remodeling. Molecular Cell, 2017, 65(5): 885-899.e6.

doi: S1097-2765(17)30046-1 pmid: 28238652
[35]
MOON M C, YAU L, WRIGHT B, ZAHRADKA P. Injury-induced expression of cytokeratins 8 and 18 by vascular smooth muscle cells requires concurrent activation of cytoskeletal and growth factor receptors. Canadian Journal of Physiology and Pharmacology, 2008, 86(5): 223-231.

doi: 10.1139/Y08-019 pmid: 18432282
[36]
MANNERY Y O, MCCLAIN C J, VOS M B. Keratin 18, apoptosis, and liver disease in children. Current Pediatric Reviews, 2011, 7(4): 310-315.

pmid: 25346653
[37]
WEI Y J, CHIANG W C, SUMPTER R Jr, MISHRA P, LEVINE B. Prohibitin 2 is an inner mitochondrial membrane mitophagy receptor. Cell, 2017, 168(1/2): 224-238.e10.

doi: 10.1016/j.cell.2016.11.042
[38]
YAN C J, GONG L L, CHEN L, XU M, ABOU-HAMDAN H, TANG M L, DÉSAUBRY L, SONG Z Y. PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5- PINK1 axis. Autophagy, 2020, 16(3): 419-434.

doi: 10.1080/15548627.2019.1628520
[39]
SATELLI A, LI S L. Vimentin in cancer and its potential as a molecular target for cancer therapy. Cellular and Molecular Life Sciences, 2011, 68(18): 3033-3046.

doi: 10.1007/s00018-011-0735-1 pmid: 21637948
[40]
ZHANG X, WANG X G, LIU T X, MO M, AO L, LIU J Y, CAO J, CUI Z H. ZnSO4 rescued vimentin from collapse in DBP-exposed Sertoli cells by attenuating ER stress and apoptosis. Toxicology in Vitro, 2018, 48: 195-204.

doi: 10.1016/j.tiv.2018.01.020
[41]
FEDERTI E, MATTÉ A, GHIGO A, ANDOLFO I, JAMES C, SICILIANO A, LEBOEUF C, JANIN A, MANNA F, CHOI S Y, IOLASCON A, BENEDUCE E, MELISI D, KIM D W, LEVI S, DE FRANCESCHI L. Peroxiredoxin-2 plays a pivotal role as multimodal cytoprotector in the early phase of pulmonary hypertension. Free Radical Biology and Medicine, 2017, 112: 376-386.

doi: S0891-5849(17)30720-7 pmid: 28801243
[42]
HOSSAIN G S, LYNN E G, MACLEAN K N, ZHOU J, DICKHOUT J G, LHOTÁK S, TRIGATTI B, CAPONE J, RHO J, TANG D M, MCCULLOCH C A, AL-BONDOKJI I, MALLOY M J, PULLINGER C R, KANE J P, LI Y H, SHIFFMAN D, AUSTIN R C. Deficiency of TDAG51 protects against atherosclerosis by modulating apoptosis, cholesterol efflux, and peroxiredoxin-1 expression. Journal of the American Heart Association, 2013, 2(3): e000134.

doi: 10.1161/JAHA.113.000134
[43]
DAMAS J, BOURDON V. The release of glandular kallikrein from submaxillary glands of rats exposed to heat. Archives Internationales De Physiologie, De Biochimie et De Biophysique, 1994, 102(3): 183-188.

doi: 10.3109/13813459409007535
[44]
HORIUCHI Y, NAKATSU D, KANO F, MURATA M. Pyruvate kinase M1 interacts with A-Raf and inhibits endoplasmic reticulum stress-induced apoptosis by activating MEK1/ERK pathway in mouse insulinoma cells. Cellular Signalling, 2017, 38: 212-222.

doi: S0898-6568(17)30196-1 pmid: 28743549
[45]
GARCÍA-DOMINGO D, RAMÍREZ D, GONZÁLEZ DE BUITRAGO G, MARTÍNEZ-A C. Death inducer-obliterator 1 triggers apoptosis after nuclear translocation and caspase upregulation. Molecular and Cellular Biology, 2003, 23(9): 3216-3225.

doi: 10.1128/MCB.23.9.3216-3225.2003
[46]
CAO H H, WANG L L, GENG C K, YANG M, MAO W W, YANG L L, MA Y, HE M, ZHOU Y Y, LIU L Q, HU X J, YU J X, SHEN X F, GU X Z, YIN L F, SHEN Z L. In leukemia, knock-down of the death inducer-obliterator gene would inhibit the proliferation of endothelial cells by inhibiting the expression of CDK6 and CCND1. PeerJ, 2022, 10: e12832.

doi: 10.7717/peerj.12832
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