14-3-3蛋程中的互作靶蛋白鉴定及其对外源激素的响应

doi:10.3864/j.issn.0578-1752.2021.12.004

Abstract

Abstract:

【Objective】Grain yield and quality of rice mainly depend on grain filling. The family of 14-3-3 proteins is the important regulator of signaling in plant, and plays an important role in plant growth and development. This study was performed to assess the gene expression pattern and to identify client proteins, which would result in important information toward understanding the functional mechanism of 14-3-3 protein during rice grain filling. 【Method】 The expression pattern of each 14-3-3 genes at different grain filling stages were examined by the Real-time RT-PCR (qRT-PCR), and the family members GF14b and GF14e were used for subsequent functional analysis. The function motif of GF14b and GF14e protein were analyzed by using the KEGG database. The amplified open reading frames of GF14b and GF14e were cloned into the PGEX-6P-1 expression vector for production of the N-terminal-tagged fusion proteins GST-GF14b and GST-GF14e. The client proteins of GF14b and GF14e were captured with the affinity chromatography approach and identified by the LC-MS/MS. The protein interaction between client proteins and GF14b and GF14e were validated by the GST Pull-down in vitro. Phosphorylation sites and functions of all client proteins were analyzed using the KinasePhos online website and MapMan software (Version 3.60), respectively. The exogenous hormones (25×10 ^-6mol·L^-1 ABA,10×10^-6mol·L^-1 IAA,100×10^-6mol·L^-1 GA,50×10^-6mol·L^-1 ZR and 2×10^-4mol·L^-1 BR ) were sprayed on rice grains at 15 days after flowering to investigate the effects of exogenous hormones treatment on the expression of GF14b, GF14e and their interacting genes. 【Result】 Except for the GF14h, the remaining seven 14-3-3 family genes were expressed in rice grains, and the expression of GF14b and GF14e was high and highly variable during grain filling. Two same protein motifs and three differential motifs between GF14b and GF14e were identified with the protein sequence analysis. In total, 59 GF14b- and 72 GF14e-client proteins in rice grains were captured by affinity chromatography on a glutathione-agarose affinity column. Two randomly selected proteins from these client proteins, namely SUS3 and PSA, were further validated by GST Pull-down in vitro, and the results showed that the SUS3 interacted with GF14b and GF14e, whereas PSA specially interacted with GF14e, which confirmed the accuracy of the affinity chromatography assay. Among these client proteins, fourth-three were commonly affinity-identified by the GF14b and GF14e and were predicted to be implicated in the sucrose conversion, starch synthesis, glycolysis and TCA cycle. Meanwhile, it was found GF14b specifically bounded to the nucleotide metabolism and transporters, whilst GF14e tended to interact with the proteins involving C1 metabolism. In addition, most of the identified client proteins possess the phosphorylation sites of Ser or Thr. The present results also showed that the expression of GF14b and GF14e were up-regulated and most of the starch synthesis-related interacting genes (SUS2, AGPS, AGPL, PPDK2, SBE) were down-regulated after exogenous hormones treatment. 【Conclusion】 The magnitude of change in gene expression was greater in GF14b and GF14e than the other 14-3-3 gene family members during grain filling. The GF14b and GF14e could respond the change of hormones concentration and act as a regulator to negatively regulate the expression of starch synthesis related genes through protein-protein interaction, in turn, play an important role in starch synthesis during the grain filling stage.

Key words: rice, grain filling, 14-3-3 protein, protein-protein interactions, hormone

ZHANG ZhiXing,MIN XiuMei,SONG Guo,CHEN Hua,XU HaiLong,LIN WenXiong. Identification of 14-3-3 Client Proteins in Rice Grains and Their Response to Exogenous Hormones During the Grain Filling Stage[J].Scientia Agricultura Sinica, 2021, 54(12): 2523-2537.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2021.12.004

https://www.chinaagrisci.com/EN/Y2021/V54/I12/2523

Figures/Tables 9

Table 1

Fig. 1

Table 2

Fig. 2

Table 3

14-3-3 species GF14b and GF14e client proteins in developing rice grains"

登入号 Accession number^a	蛋白名称 Protein name	细胞定位 Localization^b	预测的磷酸化位点 Predicted phosphorylated site^c		互作结合 Interaction with^d
登入号 Accession number^a	蛋白名称 Protein name	细胞定位 Localization^b	Serine (S)	Threonine (T)	GF14b	GF14e
01 Sugar conversion and starch synthesis
LOC_Os01g44220	ADP-glucose pyrophosphorylase large subunit (AGPL)	Chloroplast	0	1	id	id
LOC_Os03g28330	Sucrose synthase 1 (SUS1)	Cytoplasm	5	4	id	id
LOC_Os06g09450	Sucrose synthase 2 (SUS2)	Chloroplast	3	4	id	id
LOC_Os07g42490	Sucrose synthase 3 (SUS3)	Chloroplast	5	5	id	id
LOC_Os05g33570	Pyruvate, phosphate dikinase 1 (PPDK1)	Chloroplast	5	4	id	id
LOC_Os03g31750	Pyruvate, phosphate dikinase 2 (PPDK2)	Cytoplasm	7	3	id	id
LOC_Os03g55090	Alpha-1,4 glucan phosphorylase (GP)	Cytoplasm	12	3	id	id
LOC_Os06g51084	1,4-alpha-glucan-branching enzyme (GBE)	Chloroplast	12	3	id	id
LOC_Os02g32660	Starch branching enzyme (SBE)	Chloroplast.	9	2	id	id
LOC_Os08g25734	Glucose-1-phosphate adenylyltransferase small subunit (AGPS)	Chloroplast	7	1	id	id
02 Photosynthesis
LOC_Os01g31690	Oxygen-evolving enhancer protein 1 (OEE1)	Chloroplast	3	0	id	id
LOC_Os07g04840	PsbP (PSBP)	Chloroplast	2	0	id
LOC_Os12g19470	Ribulose bisphosphate carboxylase small chain (RBCS)	Chloroplast	4	1	id
LOC_Os10g21268	Ribulose bisphosphate carboxylase large chain (RBCL)	Cytoplasm	1	1	id	id
LOC_Os12g10580	Ribulose bisphosphate carboxylase large chain (RBCL)	Chloroplast	4	2		id
LOC_Os06g04270	Putative transketolase (TK)	Chloroplast	7	4		id
03 Glycolysis
LOC_Os01g67860	Fructose-bisphosphate aldolase (FBA)	Cytoplasm	4	1	id	id
LOC_Os08g02120	Fructokinase-2 (FRK2)	Cytoplasm	0	1	id	id
LOC_Os05g33380	Fructose-bisphosphate aldolase cytoplasmic isozyme (FBA)	Cytoplasm	3	2	id	id
LOC_Os03g03720	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	Mitochondrion	0	0		id
LOC_Os08g03290	Glyceraldehyde-3-phosphate dehydrogenase 1 (GAPDH1)	Mitochondrion	2	2	id	id
LOC_Os04g40950	Glyceraldehyde-3-phosphate dehydrogenase 2 (GAPDH2)	Mitochondrion	0	2	id	id
LOC_Os02g38920	Glyceraldehyde-3-phosphate dehydrogenase 3 (GAPDH3)	Cytoplasm	0	2	id	id
LOC_Os03g50480	Phosphoglucomutase (PGM)	Chloroplast	8	3	id	id
LOC_Os06g45710	Phosphoglycerate kinase (PGK)	Cytoplasm	3	3	id	id
LOC_Os02g07260	Phosphoglycerate kinase (PGK)	Mitochondrion	3	4	id	id
LOC_Os05g41640	Phosphoglycerate kinase (PGK)	Chloroplast	3	1	id	id
LOC_Os09g38030	UDP-glucose pyrophosphorylase (UGP)	Cytoplasm	3	6	id	id
LOC_Os02g02560	UTP--glucose-1-phosphate uridylyltransferase (UGPU)	Cytoplasm	5	3		id
04 Amino acid metabolism
LOC_Os01g55540	Aspartate aminotransferase (ASAT)	Mitochondrion	2	2	id	id
LOC_Os02g14110	Aspartate aminotransferase (ASAT)	Chloroplast	1	1		id
LOC_Os10g25130	Alanine aminotransferase (ALAT)	Cytoplasm	2	2	id	id
LOC_Os12g42876	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 (MLG1)	Chloroplast	6	1	id	id
登入号 Accession number^a	蛋白名称 Protein name	细胞定位 Localization^b	预测的磷酸化位点 Predicted phosphorylated site^c		互作结合 Interaction with^d
登入号 Accession number^a	蛋白名称 Protein name	细胞定位 Localization^b	Serine (S)	Threonine (T)	GF14b	GF14e
LOC_Os04g55720	D-3-phosphoglycerate dehydrogenase (PGDH)	Chloroplast	5	2		id
LOC_Os08g09250	Lactoylglutathione lyase (GLX-I)	Cytoplasm	0	1		id
05 nucleotide metabolism
LOC_Os10g41410	Nucleoside diphosphate kinase (NDK)	Chloroplast			id
06 TCA
LOC_Os10g33800	Malate dehydrogenase (MDH)	Cytoplasm	3	1	id	id
LOC_Os08g09200	Aconitate hydratase protein (AHP)	Chloroplast	5	0		id
07 Fermentation
LOC_Os08g43190	Sorbitol dehydrogenase (SDH)	Cytoplasm	4	1	id	id
08 Protein synthesis
LOC_Os02g32030	Elongation factor (EF)	Nucleus	10	2	id	id
LOC_Os01g53900	Elongation factor (EF)	Cytoplasm	4	5		id
LOC_Os04g02820	Elongation factor (EF)	Nucleus	10	2		id
LOC_Os03g08010	Elongation factor 1-alpha (EFA1)	Cytoplasm	2	2	id	id
LOC_Os06g37440	Elongation factor 1-gamma 3 (EFG3)	Chloroplast			id
LOC_Os11g21990	Expressed protein (EP)	Nucleus	2	3		id
LOC_Os06g48750	DEAD-box ATP-dependent RNA helicase (DEAD)	Nucleus			id
LOC_Os01g13430	Importin-alpha re-exporter (IAE)	Cytoplasm	30	8		id
LOC_Os09g07510	HEAT repeat family protein (HR)	Cytoplasm	9	2		id
LOC_Os08g39140	Heat shock protein 81-1 (HSP81-1)	Cytoplasm	5	3	id	id
LOC_Os09g30412	Heat shock protein 81-2 (HSP81-2)	Cytoplasm	2	2	id	id
LOC_Os08g06100	O-methyltransferase 1 (ROMT-9)	Cytoplasm	0	2	id	id
LOC_Os07g42950	40S ribosomal protein S6 (RPS6)	Cytoplasm	6	5		id
LOC_Os02g01280	T-complex protein (TCP)	Chloroplast	6	4		id
LOC_Os06g02380	T-complex protein (TCP)	Chloroplast	9	3		id
LOC_Os03g64210	T-complex protein (TCP)	Chloroplast	0	0	id	id
09 Transporters
LOC_Os02g10800	Mitochondrial carrier protein (MCP)	Chloroplast	2	2	id
LOC_Os01g25065	Putative ATPase beta subunit (ATPG)	Mitochondrion	3	2	id
LOC_Os11g47970	AAA-type ATPase family protein (AAA)	Cytoplasm	3	4	id
10 Disease and defense
LOC_Os11g47760	DnaK family protein (DNAK)	Cytoplasm	6	7	id	id
LOC_Os12g14070	DnaK family protein (DNAK)	Chloroplast	7	8	id	id
LOC_Os03g16860	DnaK family protein (DNAK)	Cytoplasm	6	5		id
LOC_Os02g02410	DnaK family protein (DNAK)	Mitochondrion	12	8	id	id
LOC_Os08g09770	DnaK family protein (DNAK)	Mitochondrion	6	7	id
LOC_Os11g09280	Protein disulfide isomerase-like 1-1 (PDIL1-1)	Endoplasmic reticulum	3	3	id
登入号 Accession number^a	蛋白名称 Protein name	细胞定位 Localization^b	预测的磷酸化位点 Predicted phosphorylated site^c		互作结合 Interaction with^d
登入号 Accession number^a	蛋白名称 Protein name	细胞定位 Localization^b	Serine (S)	Threonine (T)	GF14b	GF14e
11 Signal transduction
LOC_Os02g36974	14-3-3-like protein GF14-e (GF14e)	Cytoplasm	5	2	id	id
LOC_Os03g50290	14-3-3-like protein GF14-f (GF14f)	Cytoplasm	2	0	id	id
LOC_Os04g38870	14-3-3-like protein GF14-b (GF14b)	Cytoplasm	6	2	id	id
LOC_Os08g33370	14-3-3-like protein GF14-c (GF14c)	Cytoplasm	2	1	id	id
LOC_Os08g37490	14-3-3-like protein GF14-a (GF14a)	Cytoplasm	2	0	id	id
LOC_Os11g34450	14-3-3-like protein GF14-d (GF14d)	Cytoplasm	5	1		id
12 Cell growth and division
LOC_Os02g12650	Puromycin-sensitive aminopeptidase (PSA)	Cytoplasm	6	2		id
LOC_Os08g30810	Puromycin-sensitive aminopeptidase (PSA)	Cytoplasm	5	4		id
LOC_Os02g02890	Peptidyl-prolyl cis-trans isomerase (CYP2)	Mitochondrion	3	4	id
LOC_Os01g59790	ADP-ribosylation factor 1 (ARF1)	Mitochondrion	0	0	id
LOC_Os03g51600	Tubulin alpha-1 chain (TUBA1)	Mitochondrion	6	1	id
LOC_Os05g34170	Tubulin beta-6 chain (TUBB6)	Cytoplasm	6	2	id	id
LOC_Os06g46000	Tubulin beta-3 chain (TUBB3)	Cytoplasm	5	3		id
LOC_Os11g14220	Tubulin alpha-2 chain (TUBA2)	Cytoplasm	7	1		id
LOC_Os12g44350	Actin-1 (ACTIN1)	Cytoplasm	2	3		id
LOC_Os03g50885	Actin-1 (ACTIN1)	Cytoplasm	4	2	id
LOC_Os11g06390	Actin-7 (ACTIN7)	Cytoplasm	7	2		id
LOC_Os10g36650	Actin-2 (ACTIN2)	Cytoplasm	7	2		id
LOC_Os01g73310	Putative actin (ACTIN)	Cytoplasm	5	2		id
LOC_Os05g01600	Actin-97(ACTIN97)	Cytoplasm	5	2		id
13 C1-metabolism
LOC_Os09g27420	Formate--tetrahydrofolate ligase (FTL)	Chloroplast	10	5		id
LOC_Os06g29180	Formate dehydrogenase 1 (FDH1)	Mitochondrion	0	3		id
14 Unknown classification
LOC_Os03g49190	Oleosin 18 kDa (OLE18)	Cytoplasm	1	0	id
LOC_Os06g49650	Harpin-induced protein 1 domain containing (HIP1)	Nucleus	3	1	id

Table 3

Fig. 3

Fig. 4

Fig. 5

Fig. 6

References 42

[1]	THOMAS D, GUTHRIDGE M, WOODCOCK J, LOPEZ A. 14-3-3 protein signaling in development and growth factor responses. Current Topics in Developmental Biology, 2005,67:285-303.
[2]	COBLITZ B, SHIKANO S, WU M, GABELLI S B, COCKRELL L M, SPIEKER M, HANYU Y, FU H, AMZEL L M, LI M. C-terminal recognition by 14-3-3 proteins for surface expression of membrane receptors. Journal of Biological Chemistry, 2005,280(43):36263-36272. doi: 10.1074/jbc.M507559200
[3]	DENISON F C, PAUL A L, ZUPANSKA A K, FERL R J. 14-3-3 proteins in plant physiology. Seminars in Cell & Developmental Biology, 2011,22(7):720-727.
[4]	WIILSON R S, SWATEK K N, THELEN J J. Regulation of the Regulators: Post-translational modifications, subcellular, and spatiotemporal distribution of plant 14-3-3 proteins. Frontiers in Plant Science, 2016,7:611.
[5]	HIMMELBACH A, YANG Y, GRILL E. Relay and control of abscisic acid signaling. Current Opinion in Plant Biology, 2003,6(5):470-479. doi: 10.1016/S1369-5266(03)00090-6
[6]	YIN Y H, VAFEADOS D, TAO Y, YOSHIDA S, ASAMI T, CHORY J. A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis. Cell, 2005,120(2):249-259. doi: 10.1016/j.cell.2004.11.044
[7]	YAO Y, DU Y, JIANG L, LIU J Y. Interaction between ACC synthase 1 and 14-3-3 proteins in rice: a new insight. Biochemistry-Moscow, 2007,72(9):1003-1007. doi: 10.1134/S000629790709012X
[8]	DIAZ C, KUSANO M, SULPICE R, ARAKI M, REDESTIG H, SAITO K, STITT M, SHIN R. Determining novel functions of Arabidopsis 14-3-3 proteins in central metabolic processes. BMC Systems Biology, 2011,5(1):192. doi: 10.1186/1752-0509-5-192
[9]	SWATEK K N, GRAHAM K, AGRAWAL G K, THELEN J J. The 14-3-3 isoforms chi and epsilon differentially bind client proteins from developing Arabidopsis seed. Journal of Proteome Research, 2011,10(9):4076-4087. doi: 10.1021/pr200263m
[10]	DOU Y, LIU X, YIN Y, HAN S, LU Y, LIU Y, HAO D. Affinity chromatography revealed insights into unique functionality of two 14-3-3 protein species in developing maize kernels. Journal of Proteomics, 2015,114:274-286. doi: 10.1016/j.jprot.2014.10.019
[11]	宋健民, 戴双, 李豪圣, 刘爱峰, 程敦公, 楚秀生, TETLOW I J, Michael J E. 小麦胚乳 14-3-3 蛋白的表达及其淀粉体淀粉合成酶的互作. 作物学报, 2009,35(8):1445-1450.
	SONG J M, DAI S, LI H S, LIU A F, CHENG D G, CHU X S, TETLOW I J, Michael J E. Expression of a wheat endosperm 14-3-3 protein and its interactions with starch biosynthetic enzymes in amyloplasts. Acta Agronomica Sinica, 2009,35(8):1445-1450. (in Chinese)
[12]	YANG J C, ZHANG J H. Grain-filling problem in 'super' rice. Journal of Experimental Botany, 2010,61(1):1-4. doi: 10.1093/jxb/erp348
[13]	康国章, 王永华, 郭天财, 朱云集, 官春云. 植物淀粉合成的调控酶. 遗传, 2006,28(1):110-116.
	KANG G Z, WANG Y H, GUO T C, ZHU Y J, GUAN C Y. Key enzymes in strach synthesis in plants. Hereditas(Beijing), 2006,28(1):110-116. (in Chinese)
[14]	黄锦文, 梁康迳, 梁义元, 林文雄. 不同类型水稻籽粒灌浆过程内源激素含量变化的研究. 中国生态农业学报, 2003,11(2):11-13.
	HUANG J W, LIANG K J, LIANG Y Y, LIN W X. Changes of endogenous hormone contents during grain development in different types of rice. Chinese Journal of Eco-Agriculture, 2003,11(2):11-13. (in Chinese)
[15]	ZHANG H, LI H W, YUAN L M, WANG Z Q, YANG J C, ZHANG J H. Post-anthesis alternate wetting and moderate soil drying enhances activities of key enzymes in sucrose-to-starch conversion in inferior spikelets of rice. Journal of Experimental Botany, 2012,63(1):215-227. doi: 10.1093/jxb/err263
[16]	YANG J C, ZHANG J H, WANG Z Q, LIU K, WANG P. Post-anthesis development of inferior and superior spikelets in rice in relation to abscisic acid and ethylene. Journal of Experimental Botany, 2006,57(1):149-160. doi: 10.1093/jxb/erj018
[17]	杨建昌, 王志琴, 朱庆森, 苏宝林. ABA 与 GA 对水稻籽粒灌浆的调控. 作物学报, 1999,25(3):341-348.
	YANG J C, WANG Z Q, ZHU Q S, SU B L. Regulation of ABA and GA to the grain filling of rice. Acta Agronomica Sinica, 1999,25(3):341-348.(in Chinese)
[18]	ZHANG Z X, CHEN J, LIN S S, LI Z, CHENG R H, FANG C X, CHEN H F, LIN W X. Proteomic and phosphoproteomic determination of ABA's effects on grain-filling of Oryza sativa L. inferior spikelets. Plant Science, 2012,185(1):259-273.
[19]	ZHANG Z X, ZHAO H, HUANG F L, LONG J F, SONG G, LIN W X. The 14-3-3 protein GF14f negatively affects grain filling of inferior spikelets of rice (Oryza sativa L.). The Plant Journal, 2019,99:344-358.
[20]	YOU C, ZHU H, XU B, HUANG W, WANG S, DING Y, LIU Z, LI G, CHEN L, DING C, TANG S. Effect of removing superior spikelets on grain filling of inferior spikelets in rice. Frontiers in Plant Science, 2016,7:1161.
[21]	CHEN F, LI Q, SUN L, HE Z. The rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress. DNA Research, 2006,13:53-63. doi: 10.1093/dnares/dsl001
[22]	YAO Y, DU Y, JIANG L, LIU J. Molecular analysis and expression patterns of the 14-3-3 gene family from Oryza Sativa. Journal of Biochemistry and Molecular Biology, 2007,40(3):349-357.
[23]	YASHVARDHINI N, BHATTACHARYA S, CHAUDHURI S, SENGUPTA D N. Molecular characterization of the 14-3-3 gene family in rice and its expression studies under abiotic stress. Planta, 2018,247:229-253. doi: 10.1007/s00425-017-2779-4
[24]	YANG J C, ZHANG J H, WANG Z Q, ZHU Q S. Hormones in the grains in relation to sink strength and postanthesis development of spikelets in rice. Plant Growth Regulation, 2003,41:185-195. doi: 10.1023/B:GROW.0000007503.95391.38
[25]	ZHANG H, TAN G L, YANG L N, YANG J C, ZHANG J H, ZHAO B H. Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiology and Biochemistry, 2009,47(3):195-204. doi: 10.1016/j.plaphy.2008.11.012
[26]	李赞堂, 王士银, 姜雯宇, 张帅, 张少斌, 徐江. 穗分化期外施24-表油菜素内酯(EBR)促进水稻源、库及籽粒灌浆的生理机制. 作物学报, 2018,44(4):581-590.
	LI Z T, WANG S Y, JIANG W Y, ZHANG S, ZHANG S B, XU J. Physiological mechanisms of promoting source, sink, and grain filling by 24-epibrassinolide (EBR) applied at panicle initiation stage of rice. Acta Agronomica Sinica, 2018,44(4):581-590. (in Chinese)
[27]	ALEXANDER R D, MORRIS P C. A proteomic analysis of 14-3-3 binding proteins from developing barley grains. Proteomics, 2006,6:1886-1896. doi: 10.1002/(ISSN)1615-9861
[28]	LIU J, SUN X, LIAO W, ZHANG J, LIANG J, XU W. Involvement of OsGF14b adaptation in the drought resistance of rice plants. Rice, 2019,12:82. doi: 10.1186/s12284-019-0346-2
[29]	PURWESTRI Y A, OGAKI Y, TAMAKI S, TSUJI H, SHIMAMOTO K. The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a. Plant and Cell Physiology, 2009,50(3):429-438. doi: 10.1093/pcp/pcp012
[30]	MANOSALVA P M, BRUCE M, LEACH J E. Rice 14-3-3 protein (GF14e) negatively affects cell death and disease resistance. The Plant Journal, 2011,68(5):777-787. doi: 10.1111/j.1365-313X.2011.04728.x
[31]	YAFFE M B, RITTINGER K, VOLINIA S, CARON P R, AITKEN A, LEFFERS H, GAMBLIN S J, SMERDON S J, CANTLEY L C. The structural basis for 14-3-3: phosphopeptide binding specificity. Cell, 1997,91(7):961-971. doi: 10.1016/S0092-8674(00)80487-0
[32]	FU H, SUBRAMANIAN R R, MASTERS S C. 14-3-3 proteins: Structure, function, and regulation. Annual Review of Pharmacology and Toxicology, 2000,40(1):617-647. doi: 10.1146/annurev.pharmtox.40.1.617
[33]	DE BOER A H, VAN KLEEFF P J, GAO J. Plant 14-3-3 proteins as spiders in a web of phosphorylation. Protoplasma, 2013,250(2):425-440. doi: 10.1007/s00709-012-0437-z
[34]	ZHU G H, YE N H, YANG J C, PENG X X, ZHANG J H. Regulation of expression of starch synthesis genes by ethylene and ABA in relation to the development of rice inferior and superior spikelets. Journal of Experimental Botany , 2011,11(62):3907-3916.
[35]	SEHNKE P C, FERL R J. Plant metabolism: Enzyme regulation by 14-3-3 proteins. Current Biology, 1996,6(11), 1403-1405. doi: 10.1016/S0960-9822(96)00742-7
[36]	CHUANG H J, SEHNKE P C, FERL R J. The 14-3-3 proteins: Cellular regulators of plant metabolism. Trends in Plant Science, 1999,4(9):367-371. doi: 10.1016/S1360-1385(99)01462-4
[37]	SEHNKE P C, CHUNG H J, WU K, FERL R J. Regulation of starch accumulation by granule-associated plant 14-3-3 proteins. Proceedings of the National Academy of Sciences of the United States, 2001,98(2):765-770.
[38]	BUSTOS D M, IGLESIAS A A. Phosphorylated non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from heterotrophic cells of wheat interacts with 14-3-3 proteins. Plant Physiology, 2003,133(4):2081-2088. doi: 10.1104/pp.103.030981
[39]	ISHIDA S, FUKAZAWA J, YUASA T, TAKAHASHI Y. Involvement of 14-3-3 signaling protein binding in the functional regulation of the transcriptional activator repression of shoot growth by gibberellins. The Plant Cell, 2004,16(10):2641-2651. doi: 10.1105/tpc.104.024604
[40]	ISHIDA S, YUASA T, NAKATA M, TAKAHASHI Y. A tobacco calcium-dependent protein kinase, CDPK1, regulates the transcription factor repression of shoot growth in response to gibberellins. The Plant Cell, 2008,20(12):3273-3288. doi: 10.1105/tpc.107.057489
[41]	AITKEN A. Post-translational modification of 14-3-3 isoforms and regulation of cellular function. Seminars in Cell & Developmental Biology, 2011,22(7):673-680.
[42]	OBSIOVA V, SILHAN J, BOURA E, TEISINGER J, OBSIL T. 14-3-3 proteins: a family of versatile molecular regulators. Physiological Research, 2008,57(3):S11-S21

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

基因 Gene	Accession No.	正向引物序列 Forward primer sequence (5′-3′)	反向引物序列 Reverse primer sequence (5′-3′)
Primer for qRT-PCR
GF14a	LOC_Os08g37490	AGCCATGAAGGAGCTGTCGC	GCTCATCCTCAGGCTTGGTT
GF14b	LOC_Os04g38870	GCTTGAATCCCACCTTGTC	AATGTCCTGAGCAGCCTTG
GF14c	LOC_Os08g33370	CGTTTGACGAAGCCATCTCC	CTAGTAGAACAGGAGAAGAATC
GF14d	LOC_Os11g34450	TGCTCTCGCAGATTTGGCTC	ATCCCCAGGCTCTTTTGGAG
GF14e	LOC_Os02g36974	GATATTGCCCTGGCAGAGTTG	GAGATATCGGAAGTCCACAGC
GF14f	LOC_Os03g50290	AGCAGCTGAGAACACTCTTG	CAGCAATAGCATCGTCGAAC
GF14g	LOC_Os01g11110	AGCGACGACCTCGTCTACAT	TGACTCTCCTTGCCCTTTGT
GF14h	LOC_Os11g39540	TTATGGCCTATCAGGCTTGG	TTCTCCTTCAGGAGCTGCAT
AGPS	LOC_Os08g25734	TTACTGGGAAGACATTGGTACC	CTCCCATGAGTAATGAGTCCTC
AGPL	LOC_Os01g44220	GGAAAGATTGAATATTGGGGGC	TCAGAGGAAAGAGTTGAACTCC
SBE	LOC_Os02g32660	GATCAGTATGAAGGAGGACTGG	ACCTACTAATGCTGCAGAATGT
SUS2	LOC_Os06g09450	GGAGAAAACCAAATACCCCAAC	CAGTGTGATTCATGGCGATAAG
PPDK2	LOC_Os03g31750	CTAGCGGAATTCTTCTCGTTTG	CAAATGCCCACCTCTAAATCAG
β-actin	LOC_4333919	CTGCGGGTATCCATGAGACT	GCAATGCCAGGGAACATAGT
Primer for PCR
GF14b	LOC_Os04g38870	TTTTTTGGATTCATGTCGGCACAGGCGGAGCTTTCC	TATATATACTCGAGCTGCCCCTCGCTGGAGTCGCGCTT
GF14e	LOC_Os02g36974	TTTCGAATTCATGTCGCAGCCTGCTGAGCTTTCCC	TTTCTCGAGCTGTCCATCTCCTGATTCGCCCTTGT
SUS3	LOC_Os07g42490	TCCGAATTCATGGGGGAAACTACTGGAGAACGTGC	TTTTCTCGAGTTTGGTGGAGGCCTCTCCCTCAATG
PSA	LOC_Os02g12650	GGGGGGGAATTCATGCATGGCTTCTACAGAAGTGTG	GAGCTCGAGGTGGTCGTGAGAAATTTCCTTGAGGAC

功能位点 Functional site	功能位点位置 Functional site location
功能位点 Functional site	GF14b	GF14e
pf:IFT20	5-55
pf:DUF885	16-149	6-150
pf:14-3-3	9-245	9-245
pf:TPR_12	135-207	135-207
pf:FlaF		192-254

Identification of 14-3-3 Client Proteins in Rice Grains and Their Response to Exogenous Hormones During the Grain Filling Stage

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 9

References 42

Related Articles 15

Metrics

Comments

Recommended 0

[1]	XIAO DeShun, XU ChunMei, WANG DanYing, ZHANG XiuFu, CHEN Song, CHU Guang, LIU YuanHui. Effects of Rhizosphere Oxygen Environment on Phosphorus Uptake of Rice Seedlings and Its Physiological Mechanisms in Hydroponic Condition [J]. Scientia Agricultura Sinica, 2023, 56(2): 236-248.
[2]	ZHANG XiaoLi, TAO Wei, GAO GuoQing, CHEN Lei, GUO Hui, ZHANG Hua, TANG MaoYan, LIANG TianFeng. Effects of Direct Seeding Cultivation Method on Growth Stage, Lodging Resistance and Yield Benefit of Double-Cropping Early Rice [J]. Scientia Agricultura Sinica, 2023, 56(2): 249-263.
[3]	ZHANG Wei,YAN LingLing,FU ZhiQiang,XU Ying,GUO HuiJuan,ZHOU MengYao,LONG Pan. Effects of Sowing Date on Yield of Double Cropping Rice and Utilization Efficiency of Light and Heat Energy in Hunan Province [J]. Scientia Agricultura Sinica, 2023, 56(1): 31-45.
[4]	FENG XiangQian,YIN Min,WANG MengJia,MA HengYu,CHU Guang,LIU YuanHui,XU ChunMei,ZHANG XiuFu,ZHANG YunBo,WANG DanYing,CHEN Song. Effects of Meteorological Factors on Quality of Late Japonica Rice During Late Season Grain Filling Stage Under ‘Early Indica and Late Japonica’ Cultivation Pattern in Southern China [J]. Scientia Agricultura Sinica, 2023, 56(1): 46-63.
[5]	SANG ShiFei,CAO MengYu,WANG YaNan,WANG JunYi,SUN XiaoHan,ZHANG WenLing,JI ShengDong. Research Progress of Nitrogen Efficiency Related Genes in Rice [J]. Scientia Agricultura Sinica, 2022, 55(8): 1479-1491.
[6]	GUI RunFei,WANG ZaiMan,PAN ShengGang,ZHANG MingHua,TANG XiangRu,MO ZhaoWen. Effects of Nitrogen-Reducing Side Deep Application of Liquid Fertilizer at Tillering Stage on Yield and Nitrogen Utilization of Fragrant Rice [J]. Scientia Agricultura Sinica, 2022, 55(8): 1529-1545.
[7]	LIAO Ping,MENG Yi,WENG WenAn,HUANG Shan,ZENG YongJun,ZHANG HongCheng. Effects of Hybrid Rice on Grain Yield and Nitrogen Use Efficiency: A Meta-Analysis [J]. Scientia Agricultura Sinica, 2022, 55(8): 1546-1556.
[8]	HAN XiaoTong,YANG BaoJun,LI SuXuan,LIAO FuBing,LIU ShuHua,TANG Jian,YAO Qing. Intelligent Forecasting Method of Rice Sheath Blight Based on Images [J]. Scientia Agricultura Sinica, 2022, 55(8): 1557-1567.
[9]	GAO JiaRui,FANG ShengZhi,ZHANG YuLing,AN Jing,YU Na,ZOU HongTao. Characteristics of Organic Nitrogen Mineralization in Paddy Soil with Different Reclamation Years in Black Soil of Northeast China [J]. Scientia Agricultura Sinica, 2022, 55(8): 1579-1588.
[10]	ZHU DaWei,ZHANG LinPing,CHEN MingXue,FANG ChangYun,YU YongHong,ZHENG XiaoLong,SHAO YaFang. Characteristics of High-Quality Rice Varieties and Taste Sensory Evaluation Values in China [J]. Scientia Agricultura Sinica, 2022, 55(7): 1271-1283.
[11]	ZHAO Ling, ZHANG Yong, WEI XiaoDong, LIANG WenHua, ZHAO ChunFang, ZHOU LiHui, YAO Shu, WANG CaiLin, ZHANG YaDong. Mapping of QTLs for Chlorophyll Content in Flag Leaves of Rice on High-Density Bin Map [J]. Scientia Agricultura Sinica, 2022, 55(5): 825-836.
[12]	JIANG JingJing,ZHOU TianYang,WEI ChenHua,WU JiaNing,ZHANG Hao,LIU LiJun,WANG ZhiQin,GU JunFei,YANG JianChang. Effects of Crop Management Practices on Grain Quality of Superior and Inferior Spikelets of Super Rice [J]. Scientia Agricultura Sinica, 2022, 55(5): 874-889.
[13]	ZHANG YaLing, GAO Qing, ZHAO Yuhan, LIU Rui, FU Zhongju, LI Xue, SUN Yujia, JIN XueHui. Evaluation of Rice Blast Resistance and Genetic Structure Analysis of Rice Germplasm in Heilongjiang Province [J]. Scientia Agricultura Sinica, 2022, 55(4): 625-640.
[14]	WANG YaLiang,ZHU DeFeng,CHEN RuoXia,FANG WenYing,WANG JingQing,XIANG Jing,CHEN HuiZhe,ZHANG YuPing,CHEN JiangHua. Beneficial Effects of Precision Drill Sowing with Low Seeding Rates in Machine Transplanting for Hybrid Rice to Improve Population Uniformity and Yield [J]. Scientia Agricultura Sinica, 2022, 55(4): 666-679.
[15]	CHEN TingTing, FU WeiMeng, YU Jing, FENG BaoHua, LI GuangYan, FU GuanFu, TAO LongXing. The Photosynthesis Characteristics of Colored Rice Leaves and Its Relation with Antioxidant Capacity and Anthocyanin Content [J]. Scientia Agricultura Sinica, 2022, 55(3): 467-478.