Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (13): 2699-2709.doi: 10.3864/j.issn.0578-1752.2021.13.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

CRISPR/Cas9 Targeted Editing of OsIAA11 in Rice

LI ZhaoWei1(),LING DongLan1,SUN CongYing1,ZENG HuiLing1,LIU KaiJi1,LAN YingShan1,FAN Kai2,LIN WenXiong1()   

  1. 1College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002
    2College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002
  • Received:2020-12-03 Revised:2021-01-21 Online:2021-07-01 Published:2021-07-12
  • Contact: WenXiong LIN E-mail:lizw197@163.com;wenxiong181@163.com

RichHTML

66

PDF

700

Abstract:

【Objective】Auxin signaling pathway regulated by OsIAA11 plays an important role in the plant growth and response to various environment stresses, impacting on the final grain yield during the late stage. To explore the effect of OsIAA11 mutation on the yield, the OsIAA11 mutant plant was obtained using CRISPR/Cas9 system, and the agronomic characters of mutant plants were investigated in the field. 【Method】According to the principle of CRISPR/Cas9 technology, two 20 bp targeted sequences were designed in the first exon and second exon of the OsIAA11 genome sequence. The gene shuffling of non-specific target sites was eliminated using the blast analysis. The oligonucleotides of two target sites were inserted into the pYLgRNA-U6a and pYLgRNA-U6b plasmid vector, and then were amplified twice by PCR technology to construct U6a-IAA11-T1 and U6b-IAA11-T2 expression-boxes. The recombinant pYLCRISPR/Cas9-IAA11-T12 vector was obtained by linking two expression-boxes to pYLCRISPR/Cas9-MT vector. The pYLCRISPR/Cas9-IAA11-T12 vector was transformed into the callus ofjaponica rice ZH11 through Agrobacterium-mediated method. The T0 generation of transgenic plants was obtained by tissue culture, and the positive transgenic plants were selected through PCR method. The target sites of each T2 generation from T0 positive plants were determined via PCR and sequencing method, and finally the mutated genotypes were identified. Meanwhile, agronomic traits of the T2 generation were investigated in the field. 【Result】The pYLCRISPR/Cas9-IAA11-T12 expression vector was successfully transformed into the callus of ZH11. 25 transgenic lines were obtained, and 20 transgenic lines were identified as the positive plants. After analysis of T 2 transgenic plants via PCR and sequencing the target locus, 17 different homozygous mutations were identified in the two target sites of the OsIAA11 genomic sequence. Except for the single base insertion mutation of osiaa11-20-1, osiaa11-21-2, osiaa11-23, and osiaa11-25 in target 1 and osiaa11-22-2 in target 2, other genotypes mainly deleted small fragment base in target 1 and mutated a single base in target 2. Agronomic traits results showed that 17 osiaa11 mutants did not present a significant difference in plant height, panicle length, grain number per panicle, seed setting rate, 1000-grain weight, harvest index, and grain-straw ratio in comparison with the wild type. However, the osiaa11 displayed a significant decrease in effective spike rate, suggesting a more ineffective tiller phenotype. 【Conclusion】CRISPR/Cas9 technology successfully editedOsIAA11 and 17 different homozygous mutations genotypes were obtained. The mutant showed the reduced effective spike rate and increased ineffective tillers, indicating that OsIAA11 was related to the tiller bud appearance dominated by auxin.

Key words: rice, gene editing, OsIAA11, CRISPR/Cas9 technology, osiaa11 mutant

Table 1

The primer sequences used in this study"

引物名称Primer name 引物序列Primer sequence (5′-3′) 用途Usage
OsIAA11-T1-fwd gccgCGCGCGGCTTGTCGGGATC 靶序列1构建
Construction of target site 1
OsIAA11-T1-rev aaacGATCCCGACAAGCCGCGCG
OsIAA11-T2-fwd gttgAGCTACCCGGAGTTGTCCA 靶序列2构建
Construction of target site 2
OsIAA11-T2-rev aaacTGGACAACTCCGGGTAGCT
Hyp-F ACGGTGTCGTCCATCACAGTTTGCC T0代阳性转基因植株鉴定
Identification of the T0 positive transgenic plant
Hyp-R TTCCGGAAGTGCTTGACATTGGGGA
OsIAA11-T1-F AGACCATCGACCTCAAGC 靶点1测序检测
Sequencing for target 1
OsIAA11-T1-R GCTAACTAATGGCTCTTC
OsIAA11-T2-F GACGATGATGTCAACTTT 靶点2测序检测
Sequencing for target 2
OsIAA11-T2-R GATAATGGTAGAATAACTCA
Hyp-T2-F CTATTTCTTTGCCCTCGGAC T2代纯合突变植株体内潮霉素基因检测
Detection of the gene encoded for hygromycin in the T2 homozygous mutations
Hyp-T2-R GACGTCTGTCGAGAAGTTTCTG

Fig. 1

Target sites of the gRNA in the OsIAA11 gene locus, amino acid sequence structure of OsIAA11, and constructing style of the pYLCRISPR/Cas9-IAA11-T12 vector A: Position of two gRNA targets in the OsIAA11 gene locus; B: Amino acid sequence structure of OsIAA11; C: Cloning of two gRNA cassettes into the pYLCRISPR/Cas9-MT vector. LB: Left border; RB: Right border "

Fig. 2

Sequencing results for the two target sequences inserted in pYLCRISPR/Cas9-IAA11-T12 recombinant vector"

Fig. 3

Identification of the positive transgenic plants by PCR amplification -: Negative contro; +: Positive control; M: 2000 bp DNA marker"

Fig. 4

PCR identification and sequence alignment of osiaa11 mutants in comparison with the WT A: PCR amplification results of the DNA fragments near the targets edited of OsIAA11 for partial T2 generation ofosiaa11 mutants, the fragment length of target 1 and 2 is 443 bp and 469 bp, respectively, and WT is ZH11; B: sequence alignment of the edited targets for the osiaa11 mutants and WT line. The blue letters are the target genome sequence; The red capital letters denote PAM; Dashes strikethrough indicate the deleted bases; Insertion nucleotides are shown in red lowercase letters; -: Deletion; +: Insertion; WT: Wild type "

Fig. 5

PCR detection of the gene encoded for hygromycin in the T2 homozygous mutations -: Negative control; +: Positive control; M: 2000 bp DNA marker"

Table 2

Agronomic traits of different type osiaa11 mutants and its corresponding wild type grown in paddy field "

株系
Line 		株高
Plant height
(cm) 		有效穗数
Effective panicle
number per plant 		成穗率
Effective
spike rate (%) 		穗长
Length of main
panicle (cm) 		穗粒数
Grain number
per panicle 		结实率
Seed setting
rate (%) 		千粒重
1000-grain
weight (g) 		单株产量
Grain yield
per plant (g) 		收获指数
Harvest
index 		谷草比
Grain-straw
ratio
osiaa11-1-1 69.33±4.03abcd 6.00±0.82b 37.71±4.54b 19.93±0.93bc 128.17±24.12a 87.79±3.72ab 26.22±0.41a 11.21±2.04bcd 0.46±0.031ab 0.95±0.113ab
osiaa11-1-2 70.50±2.50abcd 7.00±0.00b 44.44±5.56b 20.35±0.62abc 120.50±9.84a 78.25±16.36ab 25.92±0.78a 12.26±1.24abcd 0.46±0.004ab 0.96±0.000ab
osiaa11-8-1 77.50±1.50a 10.50±2.50ab 44.07±4.07b 22.10±1.45abc 130.50±27.98a 86.08±6.46ab 25.98±0.98a 22.45±4.55a 0.44±0.020ab 0.88±0.073ab
osiaa11-8-2 74.00±0.82ab 9.67±1.70ab 48.73±4.37b 22.15±1.27abc 128.67±33.21a 88.57±6.58ab 25.68±0.53a 18.69±1.84abc 0.46±0.012ab 0.96±0.021ab
osiaa11-11-1 69.00±4.54bc 6.33±1.80b 43.27±7.73b 22.70±1.17a 113.67±23.50a 83.63±6.67ab 24.28±1.11a 10.51±2.67d 0.46±0.038ab 0.98±0.166ab
osiaa11-11-2 74.0±0.00abc 4.00±0.00b 26.67±0.00b 21.95±0.70abc 118.25±7.50a 88.65±2.29ab 23.32±0.78a 9.85±2.48d 0.41±0.000ab 0.76±0.000ab
osiaa11-17 67.17±1.34bcd 12.00±2.00a 40.53±4.64b 20.47±1.50bc 108.50±13.91a 90.27±8.36a 23.01±1.16a 18.30±3.14a 0.44±0.042ab 0.86±0.149ab
osiaa11-19-1 69.30±2.32bc 8.00±1.26b 43.87±3.32b 20.58±0.84abc 118.70±20.27a 83.94±5.15ab 23.21±0.84a 11.72±1.19bcd 0.43±0.051ab 0.84±0.182ab
osiaa11-19-2 66.33±1.89bcd 10.33±0.94ab 43.58±2.03b 21.15±0.80abc 109.50±11.38a 87.18±5.55ab 24.43±1.02a 12.20±0.27abcd 0.38±0.033ab 0.67±0.094ab
osiaa11-20-1 64.00±1.63cd 5.67±0.47b 50.56±7.49b 21.73±1.84abc 94.17±22.56a 80.43±13.87ab 24.42±1.49a 7.11±2.92d 0.43±0.067ab 0.90±0.269ab
osiaa11-20-2 65.50±1.50bcd 6.50±0.50b 54.89±8.74b 22.58±0.74abc 125.50±29.77a 84.28±7.52ab 24.68±0.02a 11.34±2.88bcd 0.52±0.059a 1.28±0.298a
osiaa11-21-1 60.50±1.50d 7.00±0.00b 46.88±3.12b 19.82±1.84bc 86.00±4.06a 80.67±15.64ab 21.85±3.35a 6.88±1.62d 0.44±0.017ab 0.85±0.054ab
osiaa11-21-2 61.00±0.00cd 4.00±0.00b 57.14±0.00b 18.50±0.40c 78.00±8.00a 64.70±0.41b 23.95±0.0a 3.16±0.00d 0.38±0.000ab 0.72±0.000ab
osiaa11-22-1 66.00±1.87cd 8.00±0.71ab 45.75±3.29b 21.09±1.27abc 101.12±14.10a 85.04±5.89ab 23.52±0.94a 11.05±1.67cd 0.44±0.017ab 0.85±0.054ab
osiaa11-22-2 65.25±1.09cd 6.50±0.50b 40.29±4.74b 20.50±0.79abc 103.25±19.27a 88.23±6.83ab 23.18±1.24a 9.10±0.43d 0.42±0.027ab 0.79±0.086ab
osiaa11-23 64.50±0.50cd 8.00±0.00ab 39.23±2.87b 21.08±0.89abc 124.50±10.62a 84.01±7.71ab 22.40±0.80a 12.29±0.19abcd 0.35±0.037ab 0.59±0.100b
osiaa11-25 72.50±0.50abc 11.00±1.00ab 38.52±1.48b 22.92±2.10ab 144.00±47.72a 85.26±7.35ab 24.52±1.08a 20.08±2.34ab 0.35±0.032b 0.58±0.081b
WT 69.60±0.80abc 8.00±2.00b 89.78±10.50a 21.16±1.29abc 113.70±16.80a 85.42±6.88ab 22.80±1.53a 10.65±2.75d 0.47±0.036ab 1.08±0.121ab
[1] HAGEN G, GUILFOYLE T. Auxin-responsive gene expression: genes, promoters and regulatory factors. Plant Molecular Biology, 2002, 49:373-385.
doi: 10.1023/A:1015207114117
[2] BELHAJ K, CHAPARRO-GARCIA A, KAMOUN S, PATRON N J, NEKRASOV V. Editing plant genomes with CRISPR/Cas9. Current Opinion in Biotechnology, 2015, 32:76-84.
doi: 10.1016/j.copbio.2014.11.007
[3] CARROLL D, MORTON J J, BEUMER K J, SEGAL D J. Design, construction and in vitro testing of Zinc finger nucleases. Nature Protocols, 2006, 1(3):1329-1341.
doi: 10.1038/nprot.2006.231
[4] LI T, LIU B, SPALDING M H, WEEKS D P, YANG B. High- efficiency TALEN-based gene editing produces disease-resistant rice. Nature Biotechnology, 2012, 30(5):390-392.
doi: 10.1038/nbt.2199
[5] 卢俊南, 褚鑫, 潘燕平, 陈映羲, 温栾, 戴俊彪. 基因编辑技术: 进展与挑战. 中国科学院院刊, 2018, 33(11):1184-1192.
LU J N, CHU X, PAN Y P, CHEN Y X, WEN L, DAI J B. Advances and challenges in gene editing technologies. Bulletin of the Chinese Academy of Sciences, 2018, 33(11):1184-1192. (in Chinese)
[6] WANG F J, WANG C L, LIU P Q, LEI C L, HAO W, GAO Y, LIU Y G, ZHAO K J. Enhanced rice blast resistance by CRISPR/Cas9- targeted mutagenesis of the ERF transcription factor gene OsERF922. PLoS ONE, 2016, 11(4):e0154027.
doi: 10.1371/journal.pone.0154027
[7] ZHOU H, HE M, LI J, CHEN L, HUANG Z F, ZHENG S Y, ZHU L Y, NI E, JIANG D G, ZHAO B R, ZHUANG C X. Development of commercial Thermo-sensitive genic male sterile rice accelerates hybrid rice breeding using the CRISPR/Cas9-mediated TMS5 editing system. Scientific Reports, 2016, 6:37395-37406.
doi: 10.1038/srep37395
[8] ZONG Y, WANG Y P, LI C, ZHANG R, CHEN K L, RAN Y D, QIU J L, WANG D W, GAO C X. Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nature Biotechnology, 2017, 35(5):438-441.
doi: 10.1038/nbt.3811
[9] ZENSER N, ELLSMORE A, LEASURE C, CALLIS J. Auxin modulates the degradation rate of Aux/IAA proteins. Proceedings of the National Academy of Sciences of the USA, 2001, 98(20):11795-11800.
[10] KEPINSKI S, LEYSER O. Auxin-induced SCFTIR1-Aux/IAAinteraction involves stable modification of the SCFTIR1 complex. Proceedings of the National Academy of Sciences of the USA, 2004, 101(33):12381-12386.
[11] TIWARI S B, HAGEN G, GUILFOYLE T. The roles of auxin response factor domains in auxin-responsive transcription. The Plant Cell, 2003, 15(2):533-543.
doi: 10.1105/tpc.008417
[12] OUELLET F, OVERVOORDE P J, THEOLOGIS A. IAA17/AXR3: Biochemical insight into an auxin mutant phenotype. The Plant Cell, 2001, 13:829-841.
doi: 10.1105/tpc.13.4.829
[13] JAIN M, KAUR N, GARG R, THAKUR J K, TYAGI A K, KHURANA J P. Structure and expression analysis of early auxin- responsive Aux/IAA gene family in rice (Oryza sativa). Functional & Integrative Genomics, 2006, 6(1):47-59.
[14] SONG Y, WANG L, XIONG L Z. Comprehensive expression profiling analysis of OsIAA gene family in developmental processes and in response to phytohormone and stress treatments. Planta, 2009, 229:577-591.
doi: 10.1007/s00425-008-0853-7
[15] SONG Y, YOU J, XIONG L. Characterization of OsIAA1 gene, a member of rice Aux/IAA family involved in auxin and brassinosteroid hormone responses and plant morphogenesis. Plant Molecular Biology, 2009, 70(3):297-309.
doi: 10.1007/s11103-009-9474-1
[16] NAKAMURA A, UMEMURA I, GOMI K, HASEGAWA Y, KITANO H, SAZUKA T, MATSUOKA M. Production and characterization of auxin-insensitive rice by overexpression of a mutagenized rice IAA protein. The Plant Journal, 2006, 46(2):297-306.
doi: 10.1111/tpj.2006.46.issue-2
[17] JUNG H, LEE D K, CHOI Y D, KIM J K. OsIAA6, a member of the rice Aux/IAA gene family, is involved in drought tolerance and tiller outgrowth. Plant Science, 2015, 236:304-312.
doi: 10.1016/j.plantsci.2015.04.018
[18] KITOMI Y, INAHASHI H, TAKEHISA H, SATO Y, INUKAI Y. OsIAA13-mediated auxin signaling is involved in lateral root initiation in rice. Plant Science, 2012, 190:116-122.
doi: 10.1016/j.plantsci.2012.04.005
[19] JIN L, QIN Q, WANG Y, PU Y, LIU L, WEN X, JI S, WU J, WEI C, DING B, LI Y. Rice dwarf virus P2 protein hijacks auxin signaling by directly targeting the rice OsIAA10 protein, enhancing viral infection and disease development. PLoS Pathogens, 2016, 12(9):e1005847.
doi: 10.1371/journal.ppat.1005847
[20] NI J, WANG G, ZHU Z, ZHANG H, WU Y, WU P. OsIAA23- mediated auxin signaling defines postembryonic maintenance of QC in rice. The Plant Journal, 2011, 68(3):433-442.
doi: 10.1111/tpj.2011.68.issue-3
[21] ZHU Z X, LIU Y, LIU S J, MAO C Z, WU Y R, WU P. A gain-of- function mutation in OsIAA11 affects lateral root development in rice. Molecular Plant, 2012, 5(1):154-161.
doi: 10.1093/mp/ssr074
[22] LI Z, PAN X, GUO X, FAN K, LIN W. Physiological and transcriptome analyses of early leaf senescence for ospls1 mutant rice (Oryza sativa L.) during the grain-filling stage. International Journal of Molecular Sciences, 2019, 20:1098.
doi: 10.3390/ijms20051098
[23] MA X, ZHANG Q, ZHU Q, LIU W, CHEN Y, QIU R, WANG B, YANG Z, LI H, LIU Y, XIE Y, SHEN R, CHEN S, WANG Z, CHEN Y, GUO J, CHEN L, ZHAO X, DONG Z, LIU Y G. A robust CRISPR/ Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Molecular Plant, 2015, 8(8):1274-1284.
doi: 10.1016/j.molp.2015.04.007
[24] 郭韬, 余泓, 邱杰, 李家洋, 韩斌, 林鸿宣. 中国水稻遗传学研究进展与分子设计育种. 中国科学: 生命科学, 2019, 49(10):1185-1212.
GUO T, YU H, QIE J, LI J Y, HAN B, LIN H X. Advances in rice genetics and breeding by molecular design in China. Scientia Sinica Vitae, 2019, 49(10):1185-1212. (in Chinese)
[25] 宫景文, 刘文超. 2030年非洲粮食问题预测及对中国的影响. 国土资源情报, 2017, 8:31-38.
GONG J W, LIU W C. Forecast of Africa’s food problems in 2030 and its impacts on China. Land and Resources Information, 2017, 8:31-38. (in Chinese)
[26] 凌启鸿, 张洪程, 丁艳锋, 张益彬. 水稻高产技术的新发展—精确定量栽培. 中国稻米, 2005, 1:3-7.
LING Q H, ZHANG H C, DING Y F, ZHANG Y B. New development of rice high-yield technology-precise and quantitative cultivation. China Rice, 2005, 1:3-7. (in Chinese)
[27] 黄忠明, 周延彪, 唐晓丹, 赵新辉, 周在为, 符星学, 王凯, 史江伟, 李艳锋, 符辰建, 杨远柱. 基于CRISPR/Cas9技术的水稻温敏不育基因tms5突变体的构建. 作物学报, 2018, 44(6):844-851.
HUANG Z M, ZHOU Y B, TANG X D, ZHAO X H, ZHOU Z W, FU X X, WANG K, SHI J W, LI Y F, FU C J, YANG Y Z. Construction of tms5 mutants in rice based on CRISPR/Cas9 technology. Acta Agronomica Sinica, 2018, 44(6):844-851. (in Chinese)
[28] 季新, 李飞, 晏云, 孙红正, 张静, 李俊周, 彭廷, 杜彦修, 赵全志. 基于CRISPR/Cas9系统的水稻光敏色素互作因子OsPIL15基因编辑. 中国农业科学, 2017, 50(15):2861-2871.
JI X, LI F, YAN Y, SUN H Z, ZHANG J, LI J Z, PENG T, DU Y X, ZHAO Q Z. CRISPR/Cas9 system-based editing of phytochrome- interacting factor OsPIL15 . Scientia Agricultura Sinica, 2017, 50(15):2861-2871. (in Chinese)
[29] 盛夏冰, 谭炎宁, 孙志忠, 余东, 汪雪峰, 袁贵龙, 袁定阳, 段美娟. 利用CRISPR/Cas9基因组编辑技术定向降低水稻落粒性. 中国农业科学, 2018, 51(14):2631-2641.
SHENG X B, TAN Y N, SUN Z Z, YU D, WANG X F, YUAN G L, YUAN D Y, DUAN M J. Using CRISPR/Cas9-mediated targeted mutagenesis of qSH1 reduces the seed shattering in rice . Scientia Agricultura Sinica, 2018, 51(14):2631-2641. (in Chinese)
[1] XIAO DeShun, XU ChunMei, WANG DanYing, ZHANG XiuFu, CHEN Song, CHU Guang, LIU YuanHui. Effects of Rhizosphere Oxygen Environment on Phosphorus Uptake of Rice Seedlings and Its Physiological Mechanisms in Hydroponic Condition [J]. Scientia Agricultura Sinica, 2023, 56(2): 236-248.
[2] ZHANG XiaoLi, TAO Wei, GAO GuoQing, CHEN Lei, GUO Hui, ZHANG Hua, TANG MaoYan, LIANG TianFeng. Effects of Direct Seeding Cultivation Method on Growth Stage, Lodging Resistance and Yield Benefit of Double-Cropping Early Rice [J]. Scientia Agricultura Sinica, 2023, 56(2): 249-263.
[3] ZHANG Wei,YAN LingLing,FU ZhiQiang,XU Ying,GUO HuiJuan,ZHOU MengYao,LONG Pan. Effects of Sowing Date on Yield of Double Cropping Rice and Utilization Efficiency of Light and Heat Energy in Hunan Province [J]. Scientia Agricultura Sinica, 2023, 56(1): 31-45.
[4] FENG XiangQian,YIN Min,WANG MengJia,MA HengYu,CHU Guang,LIU YuanHui,XU ChunMei,ZHANG XiuFu,ZHANG YunBo,WANG DanYing,CHEN Song. Effects of Meteorological Factors on Quality of Late Japonica Rice During Late Season Grain Filling Stage Under ‘Early Indica and Late Japonica’ Cultivation Pattern in Southern China [J]. Scientia Agricultura Sinica, 2023, 56(1): 46-63.
[5] SANG ShiFei,CAO MengYu,WANG YaNan,WANG JunYi,SUN XiaoHan,ZHANG WenLing,JI ShengDong. Research Progress of Nitrogen Efficiency Related Genes in Rice [J]. Scientia Agricultura Sinica, 2022, 55(8): 1479-1491.
[6] GUI RunFei,WANG ZaiMan,PAN ShengGang,ZHANG MingHua,TANG XiangRu,MO ZhaoWen. Effects of Nitrogen-Reducing Side Deep Application of Liquid Fertilizer at Tillering Stage on Yield and Nitrogen Utilization of Fragrant Rice [J]. Scientia Agricultura Sinica, 2022, 55(8): 1529-1545.
[7] LIAO Ping,MENG Yi,WENG WenAn,HUANG Shan,ZENG YongJun,ZHANG HongCheng. Effects of Hybrid Rice on Grain Yield and Nitrogen Use Efficiency: A Meta-Analysis [J]. Scientia Agricultura Sinica, 2022, 55(8): 1546-1556.
[8] HAN XiaoTong,YANG BaoJun,LI SuXuan,LIAO FuBing,LIU ShuHua,TANG Jian,YAO Qing. Intelligent Forecasting Method of Rice Sheath Blight Based on Images [J]. Scientia Agricultura Sinica, 2022, 55(8): 1557-1567.
[9] GAO JiaRui,FANG ShengZhi,ZHANG YuLing,AN Jing,YU Na,ZOU HongTao. Characteristics of Organic Nitrogen Mineralization in Paddy Soil with Different Reclamation Years in Black Soil of Northeast China [J]. Scientia Agricultura Sinica, 2022, 55(8): 1579-1588.
[10] ZHU DaWei,ZHANG LinPing,CHEN MingXue,FANG ChangYun,YU YongHong,ZHENG XiaoLong,SHAO YaFang. Characteristics of High-Quality Rice Varieties and Taste Sensory Evaluation Values in China [J]. Scientia Agricultura Sinica, 2022, 55(7): 1271-1283.
[11] ZHAO Ling, ZHANG Yong, WEI XiaoDong, LIANG WenHua, ZHAO ChunFang, ZHOU LiHui, YAO Shu, WANG CaiLin, ZHANG YaDong. Mapping of QTLs for Chlorophyll Content in Flag Leaves of Rice on High-Density Bin Map [J]. Scientia Agricultura Sinica, 2022, 55(5): 825-836.
[12] JIANG JingJing,ZHOU TianYang,WEI ChenHua,WU JiaNing,ZHANG Hao,LIU LiJun,WANG ZhiQin,GU JunFei,YANG JianChang. Effects of Crop Management Practices on Grain Quality of Superior and Inferior Spikelets of Super Rice [J]. Scientia Agricultura Sinica, 2022, 55(5): 874-889.
[13] ZHANG YaLing, GAO Qing, ZHAO Yuhan, LIU Rui, FU Zhongju, LI Xue, SUN Yujia, JIN XueHui. Evaluation of Rice Blast Resistance and Genetic Structure Analysis of Rice Germplasm in Heilongjiang Province [J]. Scientia Agricultura Sinica, 2022, 55(4): 625-640.
[14] WANG YaLiang,ZHU DeFeng,CHEN RuoXia,FANG WenYing,WANG JingQing,XIANG Jing,CHEN HuiZhe,ZHANG YuPing,CHEN JiangHua. Beneficial Effects of Precision Drill Sowing with Low Seeding Rates in Machine Transplanting for Hybrid Rice to Improve Population Uniformity and Yield [J]. Scientia Agricultura Sinica, 2022, 55(4): 666-679.
[15] CHEN TingTing, FU WeiMeng, YU Jing, FENG BaoHua, LI GuangYan, FU GuanFu, TAO LongXing. The Photosynthesis Characteristics of Colored Rice Leaves and Its Relation with Antioxidant Capacity and Anthocyanin Content [J]. Scientia Agricultura Sinica, 2022, 55(3): 467-478.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd