柑橘溃疡病抗性相关转录因子CitMYB20的功能

doi:10.3864/j.issn.0578-1752.2020.10.007

Abstract

Abstract:

【Objective】The objective of this study is to reveal the response of CitMYB20 to Xanthomonas citri subsp. citri (Xcc) and exogenous phytohormones in different citrus varieties, evaluate the resistance of transgenic plants to citrus bacterial canker (CBC), and to verify the biological function of CitMYB20.【Method】The primer pairs were designed according to the Citrus genome, the open reading frame sequences and promoter sequences of CitMYB20 were cloned from CBC resistant variety Fortunella japonica and CBC sensitive variety Poncirus trifoliata by PCR. The cis-acting elements of promoter sequences were predicted using online software PlantCARE. The detached mature leaves from F. japonica and P. trifoliata were inoculated with Xcc by acupuncture method, and collected at 0, 1, 3, and 5 d after inoculation. The citrus leaves were collected after being treated with exogenous salicylic acid, methyl jasmonate, and ethephon respectively for 0, 12, 24, 36, and 48 h. The expression of CitMYB20 was detected by qRT-PCR using the above treated leaves. The over-expressed vector and RNA interference vector of CitMYB20 were constructed through inserting the target sequences into the pLGNe plasmid. The transgenic plants were obtained through Agrobacterium tumefaciens, and the mature leaves of transgenic lines were randomly selected to conduct resistance evaluation against CBC.【Result】The open reading frames of CitMYB20 in F. japonica (GenBank number MN689609) and P. trifoliata (GenBank number MN689608) were highly conservative, with a similarity of 99%. There were abscisic acid-responsive and methyl jasmonate-responsive cis-acting elements in the promoter sequence of CitMYB20 from two varieties of citrus. However, there was a salicylic acid-responsive element in the promoter sequence from F. japonica (GenBank number MN689611), but not in the sequence from P. trifoliata (GenBank number MN689610). At the 5th day of Xcc in vitro inoculation, the expression of CitMYB20 in mature leaves of F. japonica was increased significantly, reaching 2.5 times of the healthy control, while the expression of CitMYB20 in P. trifoliata did not show significantly change during the whole infection period. During exogenous hormone treatment, the response of CitMYB20 to salicylic acid and methyl jasmonate was similar, that is, it showed the trend of first increasing and then decreasing in F. japonica, while the expression of CitMYB20 in P. trifoliata was decreased firstly and then returned to normal. When the leaves were treated with exogenous ethephon, the expression of CitMYB20 in F. japonica was decreased slightly, while in P. trifoliata, it showed the trend of first increasing and then decreasing. A total of 7 over-expressed plants and 5 RNA-interfered ones were obtained through genetic transformation. There was no significant difference in morphological development between transgenic plants and control plants. The results of disease resistance evaluation showed that the CitMYB20 over-expressed plants could reduce the symptom of CBC, while the RNA-interfered plants were more sensitive to Xcc.【Conclusion】The expression of CitMYB20 was induced by Xcc in CBC resistant variety. CitMYB20 is a positive regulatory transcription factor against Xcc, its function against CBC may require salicylic acid and jasmonic acid signaling pathway. CitMYB20 can be used as a candidate gene for genetically modified breeding against CBC.

Key words: Xanthomonas citri subsp. citri (Xcc), citrus bacterial canker (CBC), R2R3-MYB transcription factor, overexpression, RNA interference (RNAi), disease resistance

YAO LiXiao,FAN HaiFang,ZHANG QingWen,HE YongRui,XU LanZhen,LEI TianGang,PENG AiHong,LI Qiang,ZOU XiuPing,CHEN ShanChun. Function of Citrus Bacterial Canker Resistance-Related Transcription Factor CitMYB20[J].Scientia Agricultura Sinica, 2020, 53(10): 1997-2008.

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References 39

[1]	FERENCE C M, GOCHEZ A M, BEHLAU F, WANG N, GRAHAM J H, JONES J B . Recent advances in the understanding of Xanthomonas citri ssp. citri pathogenesis and citrus canker disease management. Molecular Plant Pathology, 2018,19(6):1302-1318.
[2]	姚利晓, 何永睿, 邹修平, 雷天刚, 许兰珍, 彭爱红, 陈善春 . 柑橘基因工程育种研究策略及其进展. 果树学报, 2013,30(6):1056-1064.
	YAO L X, HE Y R, ZOU X P, LEI T G, XU L Z, PENG A H, CHEN S C . Advances and strategies in citrus genetic engineering and breeding. Journal of Fruit Science, 2013,30(6):1056-1064. (in Chinese)
[3]	PENG A, CHEN S, LEI T, XU L, HE Y, WU L, YAO L, ZOU X . Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus. Plant Biotechnology Journal, 2017,15:1509-1519.
[4]	KOBAYASHI A K, VIEIRA L G E, BESPALHOK FILHO J C, LEITE JR. R P, PEREIRA L F P, MOLINARI H B C, MARQUES V V . Enhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene. European Journal of Plant Pathology, 2017,149(4):865-873.
[5]	OMAR A A, MURATA M M, EL-SHAMY H A, GRAHAM J H, GROSSER J W . Enhanced resistance to citrus canker in transgenic mandarin expressing Xa21 from rice. Transgenic Research, 2018,27(2):179-191.
[6]	胡安华, 祁静静, 张庆雯, 陈善春, 邹修平, 许兰珍, 彭爱红, 雷天刚, 姚利晓, 龙琴, 何永睿, 李强 . 柑橘溃疡病相关基因CsPGIP的克隆与表达. 中国农业科学, 2019,52(4):639-650.
	HU A H, QI J J, ZHANG Q W, CHEN S C, ZOU X P, XU L Z, PENG A H, LEI T G, YAO L X, LONG Q, HE Y R, LI Q . Cloning and expression analysis of the citrus bacterial canker-related gene CsPGIP in citrus. Scientia Agricultura Sinica, 2019,52(4):639-650. (in Chinese)
[7]	邹修平, 龙俊宏, 彭爱红, 陈敏, 龙琴, 陈善春 . 超量表达CsGH3.6通过抑制生长素信号转导增强柑橘溃疡病抗性. 中国农业科学, 2019,52(21):3806-3818.
	ZOU X P, LONG J H, PENG A H, CHEN M, LONG Q, CHEN S C . Overexpression of CsGH3.6 enhanced resistance to citrus canker disease by inhibiting auxin signaling transduction. Scientia Agricultura Sinica, 2019,52(21):3806-3818. (in Chinese)
[8]	HE Y, JIA R, QI J, CHEN S, LEI T, XU L, PENG A, YAO L, LONG Q, LI Z, LI Q . Functional analysis of citrus AP2 transcription factors identified CsAP2-09 involved in citrus canker disease response and tolerance. Gene, 2019,707:178-188.
[9]	LI Q, JIA R R, DOU W, QI J, QIN X, FU Y, HE Y, CHEN S . CsBZIP40, a BZIP transcription factor in sweet orange, plays a positive regulatory role in citrus bacterial canker response and tolerance. PLoS ONE, 2019,14(10):e0223498.
[10]	窦万福, 祁静静, 胡安华, 陈善春, 彭爱红, 许兰珍, 雷天刚, 姚利晓, 何永睿, 李强 . GST pull-down联合LC-MS/MS筛选柑橘抗溃疡病转录因子CsBZIP40的互作蛋白. 中国农业科学, 2019,52(13):2243-2255.
	DOU W F, QI J J, HU A H, CHEN S C, PENG A H, XU L Z, LEI T G, YAO L X, HE Y R, LI Q . Screening of interacting proteins of anti-canker transcription factor CsBZIP40 in citrus by GST pull-down combined with LC-MS/MS. Scientia Agricultura Sinica, 2019,52(13):2243-2255. (in Chinese)
[11]	CAO Z H, ZHANG S Z, WANG R K, ZHANG R F, HAO Y J . Genome wide analysis of the apple MYB transcription factor family allows the identification of MdoMYB121 gene confering abiotic stress tolerance in plants. PLoS ONE, 2013,8(7):e69955.
[12]	WILKINS O, NAHAL H, FOONG J, PROVART N J, CAMPBELL M M . Expansion and diversification of the Populus R2R3-MYB family of transcription factors. Plant Physiology, 2009,149(2):981-993.
[13]	STRACKE R, WERBER M, WEISSHAAR B . The R2R3-MYB gene family in Arabidopsis thaliana. Current Opinion in Plant Biology, 2001,4(5):447-456.
[14]	ALBERT N W, THRIMAWITHANA A H, MCGHIE T K, CLAYTON W A, DEROLES S C, SCHWINN K E, BOWMAN J L, JORDAN B R, DAVIES K M . Genetic analysis of the liverwort Marchantia polymorpha reveals that R2R3MYB activation of flavonoid production in response to abiotic stress is an ancient character in land plants. New Phytologist, 2018,218(2):554-566.
[15]	ZHAI Y, LI P, MEI Y, CHEN M, CHEN X, XU H, ZHOU X, DONG H, ZHANG C, JIANG W . Three MYB genes co-regulate the phloem-based defence against English grain aphid in wheat. Journal of Experimental Botany, 2017,68(15):4153-4169.
[16]	WEI X, SHAN T, HONG Y, XU H, LIU X, ZHANG Z . TaPIMP2, a pathogen-induced MYB protein in wheat, contributes to host resistance to common root rot caused by Bipolaris sorokiniana. Scientific Reports, 2017,7:1754.
[17]	WANG L, RAN L, HOU Y, TIAN Q, LI C, LIU R, FAN D, LUO K . The transcription factor MYB115 contributes to the regulation of proanthocyanidin biosynthesis and enhances fungal resistance in poplar. New Phytologist, 2017,215(1):351-367.
[18]	SHEN X, GUO X, GUO X, ZHAO D, ZHAO W, CHEN J, LI T . PacMYBA, a sweet cherry R2R3-MYB transcription factor, is a positive regulator of salt stress tolerance and pathogen resistance. Plant Physiology and Biochemistry, 2017,112:302-311. doi: 10.1016/j.plaphy.2017.01.015
[19]	LI T, ZHANG X Y, HUANG Y, XU Z S, WANG F, XIONG A S . An R2R3-MYB transcription factor, S1MYB28, involved in the regulation of TYLCV infection in tomato. Scientia Horticulturae, 2018,237:192-200.
[20]	XIE R, LI Y, HE S, ZHENG Y, YI S, LV Q, DENG L . Genome-wide analysis of citrus R2R3MYB genes and their spatiotemporal expression under stresses and hormone treatments. PLoS ONE, 2014,9(12):e113971.
[21]	LIU C, WANG X, XU Y, DENG X, XU Q . Genome-wide analysis of the R2R3-MYB transcription factor gene family in sweet orange ( Citrus sinensis). Molecular Biology Reports, 2014,41(10):6769-6785.
[22]	JIA N, LIU J, SUN Y, TAN P, CAO H, XIE Y, WEN B, GU T, LIU J, LI M, HUANG Y, LU J, JIN N, SUN L, XIN F, FAN B . Citrus sinensis MYB transcription factors CsMYB330 and CsMYB308 regulate fruit juice sac lignification through fine-tuning expression of the Cs4CL1 gene. Plant Science, 2018,277:334-343.
[23]	HUANG D, WANG X, TANG Z, YUAN Y, XU Y, HE J, JIANG X, PENG S A, LI L, BUTELLI E, DENG X, XU Q . Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus. Nature Plants, 2018,4(11):930-941.
[24]	JIA N, LIU J, TAN P, SUN Y, LV Y, LIU J, SUN J, HUANG Y, LU J, JIN N, LI M, IMAM K M S U, XIN F, FAN B . Citrus sinensis MYB transcription factor CsMYB85 induce fruit juice sac lignification through interaction with other CsMYB transcriptionfactors. Frontiers in Plant Science, 2019, 10: Article 213.
[25]	HUANG D, YUAN Y, TANG Z, HUANG Y, KANG C, DENG X, XU Q . Retrotransposon promoter of Ruby1 controls both light- and cold-induced accumulation of anthocyanins in blood orange. Plant, Cell and Environment, 2019,42(11):3092-3104.
[26]	ROMERO-ROMERO J L, INOSTROZA-BLANCHETEAU C, ORELLANA D, AQUEA F, REYES-DIAZ M, GIL P M, PABLO MATTE J, ARCE-JOHNSON P . Stomata regulation by tissue-specific expression of the Citrus sinensis MYB61 transcription factor improves water-use efficiency in Arabidopsis. Plant Physiology and Biochemistry, 2018,130:54-60. doi: 10.1016/j.plaphy.2018.06.034
[27]	贾瑞瑞, 周鹏飞, 白晓晶, 陈善春, 许兰珍, 彭爱红, 雷天刚, 姚利晓, 陈敏, 何永睿, 李强 . 柑橘响应溃疡病菌转录因子CsBZIP40的克隆及功能分析. 中国农业科学, 2017,50(13):2488-2497. doi: 10.3864/j.issn.0578-1752.2017.13.008
	JIA R R, ZHOU P F, BAI X J, CHEN S C, XU L Z, PENG A H, LEI T G, YAO L X, CHEN M, HE Y R, LI Q . Gene cloning and expression analysis of canker-related transcription factor CsBZIP40 in citrus. Scientia Agricultura Sinica, 2017,50(13):2488-2497. (in Chinese) doi: 10.3864/j.issn.0578-1752.2017.13.008
[28]	范海芳, 彭蕴, 张庆雯, 何永睿, 邹修平, 李强, 彭爱红, 许兰珍, 雷天刚, 陈善春, 姚利晓 . 柑橘R2R3-MYB类转录因子CitMYB20的克隆与表达分析. 分子植物育种, 2019,17(10):3199-3207.
	FAN H F, PENG Y, ZHANG Q W, HE Y R, ZOU X P, LI Q, PENG A H, XU L Z, LEI T G, CHEN S C, YAO L X . Cloning and expression of citrus R2R3-MYB transcription factor CitMYB20. Molecular Plant Breeding, 2019,17(10):3199-3207. (in Chinese)
[29]	ZOU X, JIANG X, XU L, LEI T, PENG A, HE Y, YAO L, CHEN S . Transgenic citrus expressing synthesized cecropin B genes in the phloem exhibits decreased susceptibility to Huanglongbing. Plant Molecular Biology, 2017,93(4/5):341-353.
[30]	HAO G X, STOVER E, GUPTA G . Overexpression of a modified plant thionin enhances disease resistance to citrus canker and Huanglongbing (HLB). Frontiers in Plant Science, 2016, 7: Article 1078.
[31]	VAILLEAU F, DANIEL X, TRONCHET M, MONTILLET J L, TRIANTAPHYLID S C, ROBY D . A R2R3-MYB gene, AtMYB30, acts as a positive regulator of the hypersensitive cell death program in plants in response to pathogen attack. Proceedings of the National Academy of Sciences of the United States of America, 2002,99(15):10179-10184.
[32]	LIU X, YANG L, ZHOU X, ZHOU M, LU Y, MA L, MA H, ZHANG Z . Transgenic wheat expressing Thinopyrum intermedium MYB transcription factor TiMYB2R-1 shows enhanced resistance to the take-all disease. Journal of Experimental Botany, 2013,64(8):2243-2253.
[33]	LIU H, ZHOU X, DONG N, LIU X, ZHANG H, ZHANG Z . Expression of a wheat MYB gene in transgenic tobacco enhances resistance to Ralstonia solanacearum, and to drought and salt stresses. Functional and Integrative Genomics, 2011,11(3):431-443.
[34]	CHEZEM W R, MEMON A, LI F S, WENG J K, CLAY N K . SG2-type R2R3-MYB transcription factor MYB15 controls defense- induced lignification and basal immunity in Arabidopsis. The Plant Cell, 2017,29(8):1907-1926.
[35]	VLOT A C, DEMPSEY D M A, KLESSIG D F . Salicylic acid, a multifaceted hormone to combat disease. Annual Review of Phytopathology, 2009,47:177-206.
[36]	BROWSE J . Jasmonate passes muster: A receptor and targets for the defense hormone. Annual Review of Plant Biology, 2009,60:183-205.
[37]	ZOU B, JIA Z, TIAN S, WANG X, GOU Z, LÜ B, DONG H . AtMYB44 positively modulates disease resistance to Pseudomonas syringae through the salicylic acid signalling pathway in Arabidopsis. Functional Plant Biology, 2013,40(3):304-313.
[38]	MENGISTE T, CHEN X, SALMERON J, DIETRICH R . The BOTRYTIS SUSCEPTIBLE1 gene encodes an R2R3MYB transcription factor protein thatis required for biotic and abiotic stress responses in Arabidopsis. The Plant Cell, 2003,15(11):2551-2565. doi: 10.1105/tpc.014167
[39]	YU Q B, HUANG M, JIA H G, YU Y, PLOTTO A, BALDWIN E A, BAI J H, WANG N, GMITTER JR. F G . Deficiency of valencene in mandarin hybrids is associated with a deletion in the promoter region of the valencene synthase gene. BMC Plant Biology, 2019,19(1):101.

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引物名称 Primer name	用途 Amplification	引物序列 Primer sequence (5′-3′)	备注 Remark
CitMYB20-F	克隆基因开放阅读框 To clone ORF sequence	ATAGGATCCATGGGGAGGGCTCCCTG	BamH I酶切位点 BamH I restriction site
CitMYB20-R	克隆基因开放阅读框 To clone ORF sequence	CCGGAATTCCTAAAATGGTAATGTTAATGAGTCTGC	EcoR I酶切位点 EcoR I restriction site
CitMYB20-F (g)	克隆基因抑制表达片段 To clone RNAi fragment sequence	GCTCTAGAGGCGCGCCAAGCAAAACCAGAAGGCC	Xba I和Asc I酶切位点 Xba I and Asc I restriction site
CitMYB20-R (g)	克隆基因抑制表达片段 To clone RNAi fragment sequence	CGCGGATCCATTTAAATACCGATCGCAAATTCATTAAG	BamH I和Sma I酶切位点 BamH I and Sma I restriction site
CitMYB20-F (P)	克隆启动子 To clone promoter sequence	CCAGAACCTTTACTTTAATTTCTATTTTTA
CitMYB20-R (P)	克隆启动子 To clone promoter sequence	CTTGTTAATTTCTTTCAAAGTAGTGGAG
CitMYB20-F (q)	检测CitMYB20的表达量 To detect CitMYB20 expression	CTCCTCGGTCACTACTGGAGA
CitMYB20-R (q)	检测CitMYB20的表达量 To detect CitMYB20 expression	CATTAAGGCCGCCTCCGAAA
actin-f	检测actin的表达量 To detect actin expression	CATCCCTCAGCACCTTCC
actin-r	检测actin的表达量 To detect actin expression	CCAACCTTAGCACTTCTCC
35S-F	鉴定过表达植株 To identify over-expressed plant	GGAGTCAAAGATTCAAATAGAGGACCTAAC
CitMYB20-R (OE)	鉴定过表达植株 To identify over-expressed plant	TGACCACCTATTCCCCAACATGT
CitMYB20-F (RNAi)	鉴定RNAi抑制表达植株 To identify RNAi plant	ATTTGCGATCGGTATTTAAATGTGTAA
CitMYB20-R (RNAi)	鉴定RNAi抑制表达植株 To identify RNAi plant	GCTAGCCAGGATCCAAATACCTGCAAA

元件名称 Motif name	序列 Sequence	位置Position		方向Strand		功能 Function
元件名称 Motif name	序列 Sequence	枣阳小叶枳 P. trifoliata	金弹金柑 F. japonica	枣阳小叶枳 P. trifoliata	金弹金柑 F. japonica	功能 Function
ABRE	ACGTG	-1198	-1190	+	+	脱落酸响应 Abscisic acid responsiveness
CGTCA-motif	CGTCA	-321	-321	-	-	茉莉酸甲酯响应 Methyl jasmonate responsiveness
		-316		+
TGACG-motif	TGACG	-321	-321	+	+
		-316		-
TCA-element	CCATCTTTTT		-1452		-	水杨酸响应 Salicylic acid responsiveness
TCA-element	CCATCTTTTT		-1390		+	水杨酸响应 Salicylic acid responsiveness

Function of Citrus Bacterial Canker Resistance-Related Transcription Factor CitMYB20

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