苹果NLP（Nin-Like Protein）转录因子基因家族全基因组鉴定及表达模式分析

doi:10.3864/j.issn.0578-1752.2019.23.014

Abstract

Abstract:

【Objective】The study was carried out to explore the whole genome characteristics and expression patterns of NLP transcription factors in apple, and further understand its structural characteristics and mechanism.【Method】Based on the local BLAST database and Pfam database, the members of NLP transcription factor family in the whole genome of apple were identified by using two query strategies with blastp and hmmsearch. Through strict filtration and confirmation, the results were used for further analysis, and analysis mainly divides into three parts, including NLP proteins analysis, analysis of NLP genes and NLP expression analysis in apple. Programs or softwares, such as ProtParam, Clustal Omega, MEGA7, MEME 5.0.2, SOPMA, Phyre ², WoLF PSORT and STRING, were used for protein analysis, online tools included MG2C, GSDS2.0, PlantCARE, psRNATarget, were used for gene analysis, and the expression of MdNLP gene was quantitatively detected by qRT-PCR.【Result】6 NLP members were identified from apple protein databases, which were classified into three categories by phylogenetic analysis: I, II and III. The protein secondary structure was dominated by random coil, followed by alpha-helix, and the smallest proportion was beta-turn. The prediction of subcellular localization was located in the nucleus, which was consistent with the characteristics of transcription factors. Chromosome localization showed that five genes (except MDP0000584547) were located on four chromosomes. Promoter analysis revealed a large number of cis-acting elements related to hormone and stress response, suggesting that MdNLP genes might be involved in the regulation of hormone and stress signals. In addition, a nitrogen-responsive GCN4 element was also identified, further indicating that such transcription factors were closely related to nitrogen. By quantitative detection, the pattern which NLP family had high expression in stem and leaf of apple was revealed. And the expression analysis results also confirmed that MdNLP genes responded to nitrogen starvation and drought stress, and so on.【Conclusion】Through the apple genome analysis, 6 NLP transcription factors were found; the analysis of structure and conserved domain of protein for 6 gene suggested that there had a very high similarity and conservation between them, at the same time, they were different in a way; the associated protein network of NLP family in Arabidopsis thaliana were used for MdNLPs, MDP0000132856, which had the highest homology with AtNLP7, might also have complicated features and functions.

Key words: apple, NLP transcription factor, nitrogen signal, bioinformatics

WANG Xun, CHEN XiXia, LI HongLiang, ZHANG FuJun, ZHAO XianYan, HAN YuePeng, WANG XiaoFei, HAO YuJin. Genome-Wide Identification and Expression Pattern Analysis of NLP (Nin-Like Protein) Transcription Factor Gene Family in Apple[J].Scientia Agricultura Sinica, 2019, 52(23): 4333-4349.

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References 40

[1]	KONISHI M, YANAGISAWA S . Arabidopsis NIN-like transcription factors have a central role in nitrate signalling. Nature Communications, 2013,4:1617. doi: 10.1038/ncomms2621 pmid: 23511481
[2]	SCHAUSER L, ROUSSIS A, STILLER J, STOUGAARD J . A plant regulator controlling development of symbiotic root nodules. Nature, 1999,402(6758):191-195. doi: 10.1038/46058 pmid: 10647012
[3]	SCHAUSER L, WIELOCH W, STOUGAARD J . Evolution of NIN-like proteins in Arabidopsis, rice, and Lotus japonicus. Journal of Molecular Evolution, 2005,60(2):229-237. doi: 10.1007/s00239-004-0144-2
[4]	KUMAR A, BATRA R, GAHLAUT V, GAUTAM T, KUMAR S, SHARMA M, TYAGI S, SINGH K P, BALYAN H S, PANDEY R, GUPTA P K . Genome-wide identification and characterization of gene family for RWP-RK transcription factors in wheat ( Triticum aestivum L.). PLoS ONE, 2018,13(12):e0208409. doi: 10.1371/journal.pone.0208409 pmid: 30540790
[5]	GE M, LIU Y H, JIANG L, WANG Y C, LV Y D, ZHOU L, LIANG S Q, BAO H B, ZHAO H . Genome-wide analysis of maize NLP transcription factor family revealed the roles in nitrogen response. Plant Growth Regulation, 2018,84(1):95-105. doi: 10.1007/s10725-017-0324-x
[6]	吴翔宇, 许志茹, 曲春浦, 李蔚, 孙琦, 刘关君 . 毛果杨NLP基因家族生物信息学分析与鉴定. 植物研究, 2014,34(1):37-43.
	WU X Y, XU Z R, QU C P, LI W, SUN Q, LIU G J . Genome-wide identification and characterization of NLP gene family in Populus trichocarpa. Bulletin of Botanical Research, 2014,34(1):37-43. (in Chinese)
[7]	曹雄军, 卢晓鹏, 熊江, 李静, 吴倩, 周芳芳, 谢深喜 . 枳NLP转录因子克隆及其在不同水分条件下的表达. 中国农业科学, 2016,49(2):381-390. doi: 10.3864/j.issn.0578-1752.2016.02.018
	CAO X J, LU X P, XIONG J, LI J, WU Q, ZHOU F F, XIE X S . Cloning and expression of Poncirus Trifoliata (L.) Raf. NIN-Like transcription factors under different water conditions. Scientia Agricultura Sinica, 2016,49(2):381-390. (in Chinese) doi: 10.3864/j.issn.0578-1752.2016.02.018
[8]	LIU M, CHANG W, FAN Y H, SUN W, QU C M, ZHANG K, LIU L Z, XU X F, TANG Z L, LI J N, LU K . Genome-wide identification and characterization of NODULE-INCEPTION-LIKE Protein (NLP) family genes in Brassica napus. International Journal of Molecular Sciences, 2018,19(8):2270. doi: 10.1016/j.gene.2019.144275 pmid: 31809843
[9]	MARCHIVE C, ROUDIER F, CASTAINGS L, BRÉHAUT V, BLONDET E, COLOT V, MEYER C, KRAPP A, . Nuclear retention of the transcription factor NLP7 orchestrates the early response to nitrate in plants. Nature Communications, 2013,4:1713. doi: 10.1038/ncomms2650 pmid: 23591880
[10]	YAN D W, EASWARAN V, CHAU V, OKAMOTO M, IERULLO M, KIMURA M, ENDO A, YANO R, PASHA A, GONG Y C, BI Y M, PROVART N, GUTTMAN D, KRAPP A, ROTHSTEIN S J, NAMBARA E . NIN-like protein 8 is a master regulator of nitrate- promoted seed germination in Arabidopsis. Nature Communications, 2016,7:13179. doi: 10.1038/ncomms13179 pmid: 27731416
[11]	LIU K H, NIU Y J, KONISHI M, WU Y, DU H, CHUNG H S, LI L, BOUDSOCQ M, MCCORMACK M, MAEKAWA S, ISHIDA T, ZHANG C, SHOKAT K, YANAGISAWA S, SHEEN J . Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks. Nature, 2017,545(7654):311. doi: 10.1038/nature22077 pmid: 28489820
[12]	YU L H, WU J, TANG H, YUAN Y, WANG S M, WANG Y P, ZHU Q S, LI S G, XIANG C B . Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation. Scientific Reports, 2016,6:27795. doi: 10.1038/srep27795 pmid: 27293103
[13]	VELASCO R, ZHARKIKH A, AFFOURTIT J, DHINGRA A, CESTARO A, KALYANARAMAN A, FONTANA P, BHATNAGAR S K, TROGGIO M, PRUSS D, SALVI S, PINDO M, BALDI P, CASTELLETTI S, CAVAIUOLO M, COPPOLA G, COSTA F, COVA V, RI A D, GOREMYKIN V , et al. The genome of the domesticated apple (Malus×domestica Borkh.). Nature Genetics, 2010,42(10):833-839. doi: 10.1038/ng.654 pmid: 20802477
[14]	JUNG S, LEE T, CHENG C H, BUBLE K, ZHENG P, YU J, HUMANN J, FICKLIN S P, GASIC K, SCOTT K, FRANK M, RU S, HOUGH H, EVANS K, PEACE C, OLMSTEAD M, DEVETTER L W, MCFERSON J, COE M, WEGRZYN J L, STATON M E, ABBOTT A G, MAIN D . 15 years of GDR: New data and functionality in the Genome Database for Rosaceae. Nucleic Acids Research, 2018,47(D1):D1137-D1145. doi: 10.1093/nar/gky1000 pmid: 30357347
[15]	FINN R D, BATEMAN A, CLEMENTS J, COGGILL P, EBERHARDT R Y, EDDY S R, HEGER A, HETHERINGTON K, HOLM L, MISTRY J, SONNHAMMER E L L, TATE J, PUNTA M . Pfam: The protein families database. Nucleic Acids Research, 2013,42(D1):D222-D230. doi: 10.1093/nar/gkt1223 pmid: 24288371
[16]	KUMAR S, STECHER G, TAMURA K . MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 2016,33(7):1870-1874. doi: 10.1093/molbev/msw054 pmid: 27004904
[17]	BAILEY T L, WILLIAMS N, MISLEH C, LI W W . MEME: discovering and analyzing DNA and protein sequence motifs. Nucleic Acids Research, 2006,34(suppl_2):W369-W373. doi: 10.1093/nar/gkl198 pmid: 16845028
[18]	KELLEY L A, MEZULIS S, YATES C M, WASS M N, STERNBERG M J E . The Phyre2 web portal for protein modeling, prediction and analysis. Nature Protocols, 2015,10(6):845-858. doi: 10.1038/nprot.2015.053 pmid: 25950237
[19]	HORTON P, PARK K J, OBAYASHI T, FUJITA N, HARADA H, ADAMS-COLLIER C J, NAKAI K . WoLF PSORT: Protein localization predictor. Nucleic Acids Research, 2007,35(suppl_2):W585-W587. doi: 10.1104/pp.110.156851 pmid: 20647376
[20]	SZKLARCZYK D, GABLE A L, LYON D, JUNGE A, WYDER S, HUERTA-CEPAS J, SIMONOVIC M, DONCHEVA N T, MORRIS J H, BORK P, JENSEN L J, VON MERING C . STRING v11: Protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets. Nucleic Acids Research, 2018,47(D1):D607-D613. doi: 10.1093/nar/gky1131 pmid: 30476243
[21]	HU B, JIN J P, GUO A Y, ZHANG H, LUO J C, GAO G . GSDS 2.0: An upgraded gene feature visualization server. Bioinformatics, 2014,31(8):1296-1297. doi: 10.1093/bioinformatics/btu817 pmid: 25504850
[22]	LESCOT M DÉHAIS P, THIJS G, MARCHAL K, MOREAU Y, VAN DE PEER Y, ROUZÉ P, ROMBAUTS S . PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Research, 2002,30(1):325-327. doi: 10.1093/nar/30.1.325 pmid: 11752327
[23]	DAI X B, ZHUANG Z H, ZHAO P X C . psRNATarget: A plant small RNA target analysis server (2017 release). Nucleic Acids Research, 2018,46(W1):W49-W54. doi: 10.1093/nar/gky316 pmid: 29718424
[24]	LIVAK K J, SCHMITTGEN T D . Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method. Methods, 2001,25(4):402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[25]	CHARDIN C, GIRIN T, ROUDIER F, MEYER C, KRAPP A . The plant RWP-RK transcription factors: Key regulators of nitrogen responses and of gametophyte development. Journal of Experimental Botany, 2014,65(19):5577-5587. doi: 10.1093/jxb/eru261
[26]	MÜLLER M, KNUDSEN S . The nitrogen response of a barley C-hordein promoter is controlled by positive and negative regulation of the GCN4 and endosperm box. The Plant Journal, 1993,4(2):343-355. doi: 10.1046/j.1365-313x.1993.04020343.x pmid: 8220485
[27]	赵勐 . 玉米氮素营养相关小分子非编码RNA的克隆及miRNA169的功能鉴定[D]. 北京: 中国农业大学, 2014.
	ZHAO M . Cloning of small RNAs related to nitrogen nutrition in maize and functional analysis of miRNA169[D]. Beijing: China Agricultural University, 2014. (in Chinese)
[28]	许振华 . 玉米低硝酸盐响应microRNA及靶基因鉴定与验证[D]. 北京: 中国农业科学院, 2011. doi: 10.1093/aob/mct133 pmid: 23788746
	XU Z H . Identification and verification of microRNAs and their targets on response low nitrate in maize[D]. Beijing: Chinese Academy of Agricultural Sciences, 2011. (in Chinese) doi: 10.1093/aob/mct133 pmid: 23788746
[29]	严莉, 王翠平, 陈建伟, 乔改霞, 李健 . 基于转录组信息的黑果枸杞MYB转录因子家族分析. 中国农业科学, 2017,50(20):3991-4002. doi: 10.3864/j.issn.0578-1752.2017.20.013
	YAN L, WANG C P, CHEN J W, QIAO G X, LI J . Analysis of MYB transcription factor family based on transcriptome sequencing in Lycium ruthenicum Murr. Scientia Agricultura Sinica, 2017,50(20):3991-4002. (in Chinese) doi: 10.3864/j.issn.0578-1752.2017.20.013
[30]	姜秀明, 牛义岭, 许向阳 . 番茄NAC基因家族的系统进化及表达分析. 分子植物育种, 2016,14(8):1948-1964.
	JIANG X M, NIU Y L, XU X Y . Phylogenetic evolution and expression analysis of NAC gene family in tomato ( Solanum lycopersicum). Molecular Plant Breeding, 2016,14(8):1948-1964. (in Chinese)
[31]	MAO K, DONG Q L, LI C, LIU C H, MA F W . Genome wide identification and characterization of apple bHLH transcription factors and expression analysis in response to drought and salt stress. Frontiers in Plant Science, 2017,8:480. doi: 10.3389/fpls.2017.00480 pmid: 28443104
[32]	孙明岳, 周君, 谭秋平, 付喜玲, 陈修德, 李玲, 高东升 . 苹果bZIP转录因子家族生物信息学分析及其在休眠芽中的表达. 中国农业科学, 2016,49(7):1325-1345. doi: 10.3864/j.issn.0578-1752.2016.07.010
	SUN M Y, ZHOU J, TAN Q P, FU X L, CHEN X D, LI L, GAO D S . Analysis of basic leucine zipper genes and their expression during bud dormancy in apple ( Malus×domestica). Scientia Agricultura Sinica, 2016,49(7):1325-1345. (in Chinese) doi: 10.3864/j.issn.0578-1752.2016.07.010
[33]	董蔚, 邬培祥, 杨宁, 刘锡江, 宋玉光 . 紫花苜蓿盐胁迫响应WRKY转录因子的克隆及表达特征分析. 植物生理学报, 2018,54(9):1481-1489.
	DONG W, WU P X, YANG N, LIU X J, SONG Y G . Cloning and expression analysis of WRKY transcription factor involved in salinity stress in alfalfa. Plant Physiology Journal, 2018,54(9):1481-1489. (in Chinese)
[34]	王小非, 刘鑫, 苏玲, 孙永江, 张世忠, 郝玉金, 由春香 . 番茄LBD基因家族的全基因组序列鉴定及其进化和表达分析. 中国农业科学, 2013,46(12):2501-2513. doi: 10.3864/j.issn.0578-1752.2013.12.011
	WANG X F, LIU X, SU L, SUN Y J, ZHANG S Z, HAO Y J, YOU C X . Identification, evolution and expression analysis of the LBD gene family in tomato. Scientia Agricultura Sinica, 2013,46(12):2501-2513. (in Chinese) doi: 10.3864/j.issn.0578-1752.2013.12.011
[35]	CASTAINGS L, CAMARGO A, POCHOLLE D, GAUDON V, TEXIER Y, BOUTET-MERCEY S, TACONNAT L, RENOU J P, DANIEL-VEDELE F, FERNANDEZ E, MEYER C, KRAPP A . The nodule inception-like protein 7 modulates nitrate sensing and metabolism in Arabidopsis. The Plant Journal, 2009,57(3):426-435. doi: 10.1111/j.1365-313X.2008.03695.x pmid: 18826430
[36]	LIN J S, LI X L, LUO Z P, MYSORE K S, WEN J Q, XIE F . NIN interacts with NLPs to mediate nitrate inhibition of nodulation in Medicago truncatula. Nature Plants, 2018,4(11):942-952. doi: 10.1038/s41477-018-0261-3 pmid: 30297831
[37]	GUAN P Z, RIPOLL J J, WANG R H, VUONG L, BAILEY-STEINITZ L J, YE D N, CRAWFORD N M . Interacting TCP and NLP transcription factors control plant responses to nitrate availability. Proceedings of the National Academy of Sciences of the USA, 2017,114(9):2419-2424. doi: 10.1073/pnas.1615676114 pmid: 28202720
[38]	朱新宇, 吕万胜, 余春梅, 汪保华 . 根瘤感受样基因的进化: 结构歧异与功能分化. 植物学报, 2013,48(5):519-530. doi: 10.3724/SP.J.1259.2013.00519
	ZHU X Y, LÜ W S, YU C M, WANG B H . Evolution of nodular inception-like genes: Structural divergence and functional differentiation. Chinese Bulletin of Botany, 2013,48(5):519-530. (in Chinese) doi: 10.3724/SP.J.1259.2013.00519
[39]	YANAGISAWA S . Transcription factors involved in controlling the expression of nitrate reductase genes in higher plants. Plant Science, 2014,229:167-171. doi: 10.1016/j.plantsci.2014.09.006
[40]	KONISHI M, YANAGISAWA S . Emergence of a new step towards understanding the molecular mechanisms underlying nitrate-regulated gene expression. Journal of Experimental Botany, 2014,65(19):5589-5600. doi: 10.1093/jxb/eru267

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基因Gene	上游引物Forward primer (5′-3′)	下游引物Reverse primer (5′-3′)
MdNLP2	CTATGCATCGAGGAAACAGCTTG	CAATTCCCTCACCTTCCTCAAGA
MdNLP3	GAAATGGAGAAAGAGGGCTCTGA	TTCAGAAGGGCTTGGATACTTCC
MdNLP4	GTCAGTATGCTCTCGATCCTGATA	CAGTAAGATGTGTAGGATGTTGGC
MdNLP5	GCTTGCTTCTGTGGAGACATTAC	TCCAGTATGAGTGCTCTGTAAGC
MdActin	GGACAGCGAGGACATTCAGC	CTGACCCATTCCAACCATAACA

蛋白质Protein	α-螺旋α-helix	β-转角β-turn	无规则卷曲Random coil	延长链Extended strand
MdNLP1	28.69%	3.79%	53.11%	14.41%
MdNLP2	33.94%	5.57%	43.14%	17.35%
MdNLP3	23.40%	6.40%	55.89%	14.32%
MdNLP4	27.46%	5.90%	50.23%	16.41%
MdNLP5	26.52%	4.01%	55.50%	13.98%
MdNLP6	28.33%	6.27%	49.90%	15.49%

基因 Gene	登录号 Accession number	基因长度 Gene length	编码序列长度 CDS length	氨基酸数目 Size of aa	分子量 MW (D)	等电点 pI	亚细胞定位 Subcellular localization
MdNLP1	MDP0000788505	3856	2460	819	90889.49	6.25	细胞核 Nucleus
MdNLP2	MDP0000265619	9510	5121	1706	189398.44	7.9	细胞核 Nucleus
MdNLP3	MDP0000246881	9366	4152	1383	153424.77	7.88	细胞核 Nucleus
MdNLP4	MDP0000239938	8796	3990	1329	147220.18	5.5	细胞核 Nucleus
MdNLP5	MDP0000132856	4454	2949	982	107556.64	5.45	细胞核 Nucleus
MdNLP6	MDP0000584547	10212	3159	1052	117304.32	5.96	细胞核 Nucleus

GO分类GO classification	GO条目GO term	基因数目Gene number	描述Description
分子功能 Molecular Function	GO:0005524 GO:0017111 GO:0016887 GO:0000166 GO:0008270 GO:0003676	1 1 1 3 2 2	ATP结合 ATP binding 核苷三磷酸酶活性 Nucleoside-triphosphatase activity ATP酶活性 ATPase activity 核苷酸结合 Nucleotide binding 锌离子结合 Zinc ion binding 核酸结合 Nucleic acid binding
生物途径 Biological Process	GO:0006810	1	转运 Transport
细胞组分 Cellular Component	GO:0016020 GO:0016021	1 1	膜 Membrane 膜的整体成分 Integral component of membrane

基因 Gene	脱落酸 ABRE	低氧 ARE	茉莉酸甲酯 CGTCA	乙烯 ERE	赤霉素 GARE	氮素 GCN4	干旱 MBS	防御和胁迫 TC-rich repeats	水杨酸 TCA	生长素 TGA	病原菌 W-box
MdNLP1	0/1	2/0	1/1	1/1	0/1				1/0	1/0	0/1
MdNLP2	2/3	1/0	3/0				0/1	0/1	0/1
MdNLP3	0/2	0/3	0/1		0/1			0/1	0/1	1/0	0/1
MdNLP4	0/2	0/3	1/0					1/1	1/1	1/1	0/1
MdNLP5		1/1	2/0	1/2	0/1	0/1	1/0			0/1	0/1
MdNLP6	4/3	1/0	3/3	1/0							0/1

Genome-Wide Identification and Expression Pattern Analysis of NLP (Nin-Like Protein) Transcription Factor Gene Family in Apple

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靶基因 Target gene	miRNA	抑制类型 Inhibition	靶基因 Target gene	miRNA	抑制类型 Inhibition	靶基因 Target gene	miRNA	抑制类型 Inhibition
MdNLP2	mdm-miR171a	直接降解Cleavage	MdNLP5	mdm-miR169e	直接降解Cleavage	MdNLP5	mdm-miR395e	直接降解Cleavage
	mdm-miR171b	直接降解Cleavage		mdm-miR169f	直接降解Cleavage		mdm-miR395f	直接降解Cleavage
	mdm-miR395a	直接降解Cleavage		mdm-miR171c	抑制翻译Translation		mdm-miR395g	直接降解Cleavage
	mdm-miR395b	直接降解Cleavage		mdm-miR171d	抑制翻译Translation		mdm-miR395h	直接降解Cleavage
	mdm-miR395c	直接降解Cleavage		mdm-miR171e	抑制翻译Translation		mdm-miR395i	直接降解Cleavage
	mdm-miR395d	直接降解Cleavage		mdm-miR171g	抑制翻译Translation
	mdm-miR395e	直接降解Cleavage		mdm-miR171h	抑制翻译Translation
	mdm-miR395f	直接降解Cleavage		mdm-miR395a	直接降解Cleavage
	mdm-miR395g	直接降解Cleavage		mdm-miR395b	直接降解Cleavage
	mdm-miR395h	直接降解Cleavage		mdm-miR395c	直接降解Cleavage
	mdm-miR395i	直接降解Cleavage		mdm-miR395d	直接降解Cleavage

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