天然蜂粮对高脂血症大鼠血脂、抗氧化及免疫功能的影响

doi:10.3864/j.issn.0578-1752.2019.16.016

Abstract

Abstract:

【Objective】Bee bread is that the worker bee stores the collected pollen mass in the nest room, then bites the pollen mass, spits honey moist, and ferments it for 2 to 3 weeks. Bee bread provides essential nutritional supply for the development of old larvae and young bees. Although the traditional view is that the nutritional value of natural bee bread is higher than that of bee pollen, but there is no scientific basis. In order to evaluate the nutritional effect of bee bread scientifically, the effects of natural bee bread on blood lipid, antioxidation and immune function in rats were systematically studied with bee pollen as control.【Method】The natural bee bread producer designed by the Honeybee Research Institute of Jiangxi Agricultural University was used to produce natural bee bread and pollen by honey bee workers (Apis mellifera) at the flowering stage of white lotus. The hyperlipidemia rats used in this study were established by standard method. A blank control group and a high-fat model control group were prepared. Three doses of bee bread and pollen (low-dose group of 80 mg?kg ^-1, middle-dose group of 400 mg?kg ^-1, and high-dose group of 800 mg?kg ^-1) were fed to hyperlipidemia rats, respectively. After intragastric administration natural bee bread and bee pollen for 60 days, the levels of blood lipids including triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL) and high density lipoprotein (HDL-C) were measured. Additionally, antioxidant indexes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), total antioxidant capacity (T-AOC), immune factors interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) were detected in blood.【Result】Compared with the high-fat model group, low-dose bee bread treatment group significantly reduced the levels of TG, TC and LDL in the blood of hyperlipidemia rats. Middle-dose bee bread treatment group significantly increased the level of HDL-C. Low-dose bee pollen treatment group significantly reduced the levels of TG and TC, which indicated that both bee bread and bee pollen had a good effect on reducing hyperlipidemia. Additionally, low-, middle-, high-dose bee bread, as well as low- and middle-dose bee pollen significantly increased the content of TNF-α, which indicated that both bee bread and bee pollen enhanced immune responses compared with the high-fat model group. The SOD content in the blood of rats with high-dose bee pollen group increased significantly, but the differences among the three doses of bee bread groups, the low- and the middle-dose groups of bee pollen were not statistically significant compared with the high-fat model group. It suggested that the high-dose bee pollen enhanced antioxidant capacity. Compared with the high-fat model group, the contents of GSH-Px, T-AOC and MDA among the six experimental groups were not significantly different. Compared with the low- and middle-dose bee pollen groups, the middle-dose bee bread group significantly increased the content of HDL-C. Compared with the three groups of bee pollen, middle-dose bee bread group significantly increased the TNF-α content.【Conclusion】Combined with TG, TC, LDL, HDL-C and TNF-α, the effects of natural bee bread on reducing blood lipid and enhancing immunity were stronger than those of bee pollen. Natural bee bread is worthy of further development and utilization.

Key words: honeybee bread, rat, blood lipid, antioxidation, immunity

LI Zhen,LIU ZhiYong,JIANG WuJun,HE XuJiang,YAN WeiYu,ZHANG LiZhen,ZENG ZhiJiang. Effects of Natural Bee Bread on Blood Lipid, Antioxidation and Immune Function in Rats with Hyperlipidemia[J].Scientia Agricultura Sinica, 2019, 52(16): 2912-2920.

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References 47

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试验分组 Experimental grouping	甘油三酯 Triglyceride (mmol?L^-1)
C	0.839±0.086b
HM	2.899±0.199a
HB-L	2.991±0.150a
HB-M	2.987±0.165a
HB-H	2.964±0.134a
HP-L	2.981±0.197a
HP-M	2.942±0.140a
HP-H	2.951±0.106a

试验分组 Experimental grouping	甘油三酯 Triglyceride (mmol?L^-1)	总胆固醇 Total cholesterol (mmol?L^-1)	低密度脂蛋白 Low density lipoprotein (mmol?L^-1)	高密度脂蛋白 High density lipoprotein (mmol?L^-1)
C	0.98±0.11b	2.16±0.28b	0.43±0.04b	0.32±0.04a
HM	1.65±0.24a	3.24±0.44a	0.77±0.09a	0.21±0.03c
HB-L	1.01±0.16b	2.09±0.22b	0.49±0.07b	0.23±0.03bc
HB-M	1.11±0.19b	2.51±1.20ab	0.61±0.09ab	0.30±0.04ab
HB-H	1.28±0.18ab	2.38±0.32b	0.61±0.08ab	0.26±0.03abc
HP-L	0.97±0.10b	2.34±0.12b	0.64±0.05ab	0.21±0.02c
HP-M	1.28±0.17ab	2.20±0.20b	0.60±0.09ab	0.21±0.02c
HP-H	1.23±0.12ab	2.12±0.20b	0.58±0.16ab	0.28±0.03abc

试验分组Experimental grouping	超氧化物歧化酶 Superoxide dismutase (U?mL^-1)	谷胱甘肽过氧化物酶 Glutathione peroxidase (U?mL^-1)	总抗氧化活力 Total antioxidation capacity (U?mL^-1)	丙二醛 Malondialdehyde (nmol?L^-1)
C	301.26±31.10a	177.78±30.52a	143.43±4.32a	7.60±0.35a
HM	279.94±23.74a	155.20±18.97a	117.58±6.01bcd	8.05±0.38ab
HB-L	250.19±29.09a	157.41±16.23a	122.43±2.23b	9.15±0.28b
HB-M	257.71±13.85a	161.73±27.77a	108.76±3.20d	8.93±0.58b
HB-H	274.34±17.28a	156.79±25.79a	121.99±2.85bd	8.75±0.42b
HP-L	316.89±31.89ab	128.40±16.61a	113.92±2.49cd	8.47±0.22ab
HP-M	255.57±25.38a	151.23±26.13a	125.02±2.53b	8.37±0.55ab
HP-H	390.74±16.40b	183.75±10.05a	122.92±3.24bc	8.71±0.22ab

试验分组 Experimental grouping	白细胞介素-6 Interleukin 6 (pg?mL^-1)	肿瘤坏死因子-α Tumor necrosis factor α (pg?mL^-1)
C	301.24±12.13a	252.07±9.128a
HM	285.71±9.49ab	129.34±5.87d
HB-L	287.07±11.53ab	207.05±11.28b
HB-M	312.75±8.58a	248.03±17.07a
HB-H	258.51±13.25b	188.43±21.00bc
HP-L	279.81±5.12bc	193.34±13.92bc
HP-M	255.16±16.20c	187.26±15.16bc
HP-H	257.69±8.62bc	150.66±16.56cd

Effects of Natural Bee Bread on Blood Lipid, Antioxidation and Immune Function in Rats with Hyperlipidemia

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