Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (8): 1334-1340.doi: 10.3864/j.issn.0578-1752.2019.08.004

Special Issue: MALE STERILITY OF CROP

• MALE STERILITY OF CROP • Previous Articles     Next Articles

A Simplified Production Method of Hybrid F1 Seeds in Rapeseed

YANG GuangSheng,XIN Qiang,DONG FaMing,HONG DengFeng   

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070
  • Received:2018-11-27 Accepted:2019-01-20 Online:2019-04-16 Published:2019-04-26

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Abstract:

Safe and effective F1 seed production method is the key to hybrid production in rapeseed. Father parent lines with tribenuron-methyl resistance (or tolerance) and self-incompatiblity can be developed by means of backcross breeding. In the F1 hybrid seed production by using tribenuron-methyl as the male sterility induction agent, the father parent lines have no seedset by self-pollination because of their self-incompatibility, and are not affected by the chemical agent because of their tribenuron-methyl resistance (or tolerance). Therefore, the parental lines can be mixed sowing and the seeds can be mixed harvesting. Using the method we proposed, if the lines with tribenuron-methyl resistance (tolerance) and self-incompatiblity are already developed, hybrid breeding can be carried out immediately. It saves labor costs, improves seed production efficiency and increases seed purity because of the mixed sowing and mixed harvesting.

Key words: Brassica napus, hybrid, F1 seed production, chemical inducing male sterility, self-incompatibility

Fig. 1

A simplified production method of hybrid F1 seed in rapeseed: breeding and production procedure of father parent lines"

Fig.2

A simplified production method of hybrid F1 seed in rapeseed: Production procedure of parental lines A: Type1 female lines, widely used full male sterile lines in production. Tribenuron-methyl can be used as the male sterility induction agent to make male-fertile plants sterility and increase seed purity; B: Type2 female lines, Fertility segregated or instable male sterile lines; C: Type3 female lines, Conventional inbred lines and varieties"

Fig. 3

The development process of the pol CMS restorer 7-5STR with self-incompatibility and tribenuron-methyl resistance 7-5: A restorer of pol cytoplasmic male sterility; 12eRS7-4: A mutant of tribenuron-methyl resistance; SI Huaza 95F2: A self-incompatibility plant in the F2 population of Huayouza 95"

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