玉米SRO基因家族的鉴定及表达分析

doi:10.3864/j.issn.0578-1752.2018.15.016

Abstract

Abstract: 【Objective】The SRO (similar to rcd one) is a group of plant-specific proteins which have important functions in plant growth and development, and response to abiotic stress. Based on maize genome information, the SRO family genes were identified, and the sequences, gene localization, protein structure and their evolutionary relationships were analyzed. Meanwhile, the SRO family genes tissue-specific expression profiles and expression pattern under salt and drought stresses of ZmSROs were analyzed. This research can lay a foundation for researching the function of SRO family genes in the growth and response mechanisms of stress in maize. 【Method】The Arabidopsis SRO protein sequences were used as queries to identify SRO in maize genome and download all the SRO gene sequences, which information regarding the maize SRO genes, including CDS sequences , amino acid sequences and chromosome location were obtained from MaizeGDB. The gene structure, protein molecular weight, isoelectric point, secondary structure, subcellular localization, conserved domain, and conserved element were also prospected and analyzed by GSDS2.0, Expasy-protparam, SOPMA, Plant-mPLoc, EMBL-EBI, MEME bioinformatics tools. Meanwhile the homologous sequence alignment and phylogeny were analyzed by Clustalx (1.83) and MEGA6.0 software. The expression levels of ZmSROs genes in different maize tissues and under high salt and drought stresses were analyzed by real-time fluorescence quantitative PCR. 【Result】Six SRO family genes were identified from Maize genome, and designated ZmSRO1a to ZmSRO1f. ZmSROs were mapped on 1, 4, 5 and 9 chromosome, and contained two to five introns. The CDS of 6 ZmSROs varied in length from 1,215 to 1,791 bp, and their predicted protein products ranged from 404 to 596 aa, with corresponding molecular masses of 45.23 kD and 66.78 kD, and with predicted isoelectric points in the range of 7.01 to 9.17. Subcellular localization analysis showed that ZmSRO1a/ZmSRO1b/ZmSRO1c/ZmSRO1d were located in the chloroplast, ZmSRO1e was located in the peroxisome, and ZmSRO1f was located in the nucleus. Phylogentic analysis revealed that ZmSROs can be divided into three subgroups. Subgroup Ⅰ a contains ZmSRO1a/b/c, subgroups Ⅰ b contains ZmSRO1f, and subgroup Ⅰ c contains ZmSRO1d and ZmSRO1e. Conservative structure domain analysis showed that ZmSRO1a/ ZmSRO1b/ ZmSRO1c/ ZmSRO1d/ ZmSRO1e contain the PARP and RST domain, lack the WWE domain. ZmSRO1f contains the PARP and WWE domain, lacks the RST domains. A total 5 motif were identified from ZmSROs proteins，named motif 1—motif 5. ZmSRO1a/ZmSRO1b/ZmSRO1c contain all of motif, ZmSRO1d/ ZmSRO1e lack the motif 3，ZmSRO1f lacks the motif 5. Different tissues expression assay showed that all ZmSROs had specific expressions in root. The expression of ZmSRO1a/ZmSRO1b/ZmSRO1c/ZmSRO1d/ZmSRO1e in root were significantly up-regulated under high salinity treatment for 1 h. ZmSRO1a/ZmSRO1b/ZmSRO1c/ZmSRO1d/ZmSRO1e were down-regulated in shoots, However, the expression of ZmSRO1f in shoot was significantly up-regulated under high salinity treatment for 6 h. ZmSRO1e in root was up-regulated under drought stress for 1 h and that of ZmSRO1f was up-regulated for 24 h. Meanwhile, the expressions of ZmSRO1a/ ZmSRO1b/ZmSRO1d/ZmSRO1e in shoot were down-regulated under drought stress and that of ZmSRO1c and ZmSRO1f had no significant changes in shoot. 【Conclusion】Maize SRO family contains six genes, and ZmSROs genes could be classified into threes subgroups. Six of ZmSROs genes had specific expressions in root and could respond to high salt and drought stresses in varying degrees.

Key words: Zea mays, SRO gene, abiotic stress, gene expression

ZHAO QiuFang, MA HaiYang, JIA LiQiang, CHEN Shu, CHEN HongLiang. Genome-Wide Identification and Expression Analysis of SRO Genes Family in Maize[J].Scientia Agricultura Sinica, 2018, 51(15): 3009-3019.

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