Scientia Agricultura Sinica ›› 2018, Vol. 51 ›› Issue (15): 3000-3008.doi: 10.3864/j.issn.0578-1752.2018.15.015

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Follicular Development Related Genes Screening and Differential Expressed Analysis by Transcriptome Sequencing in Bovine Ovary

LI PengFei1, MENG JinZhu2, JING JiongJie3, BI XiLin3, WANG Kai3, ZHU ZhiWei1, LÜ LiHua3   

  1. 1College of Life Science, Shanxi Agricultural University, Taigu 030801, Shanxi; 2Wujiang College, Tongren University, Tongren 554300, Guizhou; 3College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, Shanxi
  • Received:2017-03-28 Online:2018-08-01 Published:2018-08-01

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Abstract: 【Objective】 The study focused on screening some genes involved in follicular development through high- throughput sequencing in bovine follicles at different physiological states. 【Method】Cows were selected for estrus synchronization, and the largest follicle and second largest follicle at predeviation during the first follicle wave were gathered by B-type ultrasonography. Thereafter, the RNA libraries were constructed and RNA sequencing was performed by Illumina platform. Compared with the database, parameters were set to screen high-expressed genes and differentially expressed genes, and then GO analysis was conducted. Further screened regulatory genes directly related to follicular development by Genecards, and qRT-PCR was performed to validate expression of screened genes associated with follicular development. 【Result】 Results showed that 263 differentially expressed genes were obtained from the two transcripts, which could be assigned into 90 groups under three categories by GO clustering analysis (biological processes, 64.4%; cell component, 17.8%; molecular function, 17.8%). Some important functional enrichment pathways were obtained, such as regulating signal transduction and cytokine response biological pathways; 10 high-expressed up-regulated and down-regulated genes were selected by gene expression analysis, CYP17A1 involved in estrogen synthesis and fetal gender development, LOC785462 participated in steroid hormone synthesis, DACH1 regulated cell apoptosis in process of cell development, and MAP3K3 regulated ERK and MEK1/2 signaling pathways. Six genes were found closely associate with follicular development by Genecards: up-regulated genes included PRSS35, PTGRF, ARID4B and GPR116, down-regulated genes included APOA1 and CPXM1. qRT-PCR results showed that expression of PRSS35 and ARID4B were significantly higher in DF than in SF (P＜0.05), and CPXM1 expression was significantly higher in SF than in DF (P＜0.01). There was no significant difference in the expression of PTGRF, GPR116 and APOA1 between DF and SF (P＞0.05), however, the expression variation trend was consistent with high-throughput sequencing results. 【Conclusion】The transcriptome sequencing results were accurate, PRSS35 and ARID4B played positive roles and CPXM1 played a negative role in regulating follicular development, genes related to bovine follicular development and important regulated pathways, which were of great significance to further study the regulation mechanism of follicular development.

Key words: bovine, follicle, transcript, PDF1, PDF2, development

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