Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (9): 1772-1780.doi: 10.3864/j.issn.0578-1752.2013.09.004

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Impact of Shading on Growth, Development and Physiological Characteristics of Trollius chinensis Bunge

 吕Jin-Hui , LI  Yan-Feng, WANG  Xuan, REN  Lei, FENG  Yan-Meng, ZHAO  Xia-Lu, ZHANG  Chun-Lai   

  1. 1.College of Forestry, Shanxi Agricultural University, Taigu 030801, Shanxi
    2.Institute for Sorghum Research, Shanxi Academy of Agricultural Sciences, Yuci 030600, Shanxi
  • Received:2012-12-12 Online:2013-05-01 Published:2013-03-12

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Abstract: 【Objective】This study investigated the effects of shading treatment on growth, development and physiological response of Trollius chinensis Bunge in order to determine the optimum light intensity and best growth period for shading treatment suitable for T. chinensis growth. 【Method】Two-year-old T. chinensis plants were subjected to shading treatments of 0, 40%, 60% and 80% of light intensity of sunshine, respectively, from 5th May to defoliation. Morphological indicators were recorded for plant height, the number of leaves, leaf area, petiole length, crown width, diameter of corolla and the number of flowers per plant. Physiological parameters were measured including the malondialdehyde (MDA) content, relative membrane conductivity, superoxide radical content, superoxide dismutase (SOD) activity, peroxidase (POD) activity, soluble protein content and chlorophyll content. The ultrastructure of leaf chloroplast derived from various shading treatments was observed by transmission electron microscopy. 【Result】 The activities of growth and development of T. chinensis were promoted by shading treatments, in which, 40% and 60% shading treatments prolonged the flowering periods, the number of flowers per plant and the diameter of corolla were also increased. The number of grana, grana thickness and the number of grana lamellae were increased significantly by 60% and 80% shading treatments, but the number of starch grains in chloroplast was significantly reduced by shading treatments. Physiological characteristics of T. chinensis varied between the duration/growth periods when shading treatments were applied. The plants showed a weak light stress response after shading treatment for 40 days. Following the increase on shading durations the chlorophyll content and SOD activity were decreased while MDA content and relative membrane conductivity were increased. Shading treatment for 80 days boosted the growth of T. chinensis. The relative conductivity and the superoxide radical were significantly lower than the control while the chlorophyll content was significantly higher than the control. The soluble protein content and the SOD activity were enhanced under 60% and 80% shading treatments. Shading treatment for 120 days inhibited plant growth, increased the relative conductivity significantly and decreased the POD activity .【Conclusion】 Full light and 40% shading treatment were beneficial to T. chinensis from May to the middle of June, but 40% and 60% shading treatments were good choice in summer. It would be advisable that there were no shading in late summer and early autumn.

Key words: Trollius chinensis Bunge , shading treatment , the growth and development , physiological response

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