common buckwheat,culm,lodging resistance,lignin,lignin monomer,"/> Lignin and Lignin Monomer Synthetic Characteristics of Culm in Common Buckwheat with Different Lodging Resistance Capabilities

Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (7): 1202-1209.doi: 10.3864/j.issn.0578-1752.2017.07.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Lignin and Lignin Monomer Synthetic Characteristics of Culm in Common Buckwheat with Different Lodging Resistance Capabilities

SHE HengZhi1, NIE Jiao1, LI YingShuang1, LIU XingBei1, HU Dan1, MA Shan3, Ciren zhuoga4, WANG Can1, WU DongQian1, RUAN RenWu1,2, YI ZeLin1,2   

  1. 1College of Agronomy and Biotechnology, Southwest University, Chongqing 400715; 2Chongqing Buckwheat Industry System Innovation Team, Chongqing 400715; 3Agriculture and Animal Husbandry Bureau of Chaya, Changdu 854000, Xizang; 4Agrotechnical station of Bomi, Linzhi 860000, Xizang
  • Received:2016-10-14 Online:2017-04-01 Published:2017-04-01

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Abstract: 【Objective】 This research aims to study the content change rule of lignin, total lignin monomer, G, S and H type lignin monomer of culm with different lodging resistance capabilities of buckwheat cultivars at different stages, so as to determine the lignin and lignin monomer synthetic characteristics.【Method】The varieties of Youqiao2 (YQ2, high lodging resistance), Xinnong1 (XN1, medium lodging resistance) and Ukraine Daliqiao (UD, sensitive to lodging) were used as test materials. The field experiment arranged in a factorial randomized complete design, with three replicates. Acetyl bromide method was used to determine the lignin content of different lodging resistance varieties at different growth stages. GC-MS method was conducted to determine the content of total lignin monomer which includes G, S and H type lignin monomer. Stems in common buckwheat were used to determine stem bending force and lodging index. 【Result】 At same growth stages, YQ2 was superior in culm breaking resistance and lodging index compared with XN1 and UD. The culm breaking resistance increased firstly and then decreased from the stage of branching to milk ripe, the maximum values arrived at full bloom stage. The lodging index among different varieties changed differently, the lodging index of YQ2 and UD increased and then decreased from branching to milk ripe stage, and the maximum values arrived at full bloom stage. The lodging index of XN1 increased gradually from branching to milk ripe stage, and the maximum values arrived at milk ripe stage. The content of culm lignin and lignin monomer increased from branching to milk ripe stage. The YQ2 contained higher lignin and lignin monomer than XN1 and UD. At the same growth stage, the content of S type monomer was higher than G type monomer, and the H type monomer was the lowest. From branching to milk ripe stage, S and G type monomer increased gradually, but H type monomer decreased gradually. Furthermore, from branching to milk ripe stage, the ratio of S/G decreased, and the ratio of G type monomer to total lignin monomer increased gradually. 【Conclusion】The main type of lignin monomer of culm in common buckwheat is S-G type. At different growth stages, the content of lignin, total lignin monomer, G, S and H type lignin monomer of high resistance varieties are higher than moderate resistance and lodging susceptible varieties. The S type lignin monomer increased quickly at branching stage, and G type lignin monomer increased in a great deal from branching to maturity stage.

Key words: common buckwheat')">common buckwheat, culm, lodging resistance, lignin, lignin monomer

[1] 陈庆富. 五个中国荞麦(Fagopyrum)种的核型分析. 广西植物, 2001, 21(2): 107-110. CHEN Q F. Karyotype analysis of five Fagopyrum species native to China. Guihaia, 2001, 21(2): 107-110. (in Chinese) 
[2] LIN R F, CHAI Y. Production, research and academic exchanges of China on buckwheat//CHAI Y, ZHANG Z W. Advances in Buckwheat Research. Yangling: Northwest A&F University Press, 2007: 7-12. (in Chinese) 
[3] 张以忠, 陈庆富. 荞麦研究的现状与展望. 种子, 2004, 23(3): 39-42. ZHANG Y Z, CHEN Q F. Status and perspective on research in buckwheat. Seed, 2004, 23(3): 39-42. (in Chinese) 
[4] 闫超. 内蒙古荞麦黄酮类化合物测定及特征研究[D]. 呼和浩特: 内蒙古农业大学, 2015. YAN C. Determination and profiling of flavonoids of Inner Mongolian buckwheat[D]. Hohhot: Inner Mongolia Agricultural University, 2015. (in Chinese) 
[5] 王鹏姬. 荞麦愈伤组织培养及其黄酮合成研究[D]. 杨凌: 西北农林科技大学, 2013. WANG P J. Research on callus culture and flavonoids biosynthesis of buckwheat [D]. Yangling: Northwest A&F University, 2013. (in Chinese) 
[6] 穆志新, 田翔, 周建萍, 师颖. 高效液相色谱法测定荞麦中芦丁含量. 山西农业科学, 2015, 43(11): 1408-1410. MU Z X, TIAN X, ZHOU J P, SHI Y. The determination of rutin content in buckwheat by HPLC. Journal of Shanxi Agricultural Sciences, 2015, 43(11): 1408-1410. (in Chinese) 
[7] 胡丹, 刘星贝, 汪灿, 杨浩, 李鹤鑫, 阮仁武, 袁晓辉, 易泽林. 不同抗倒性甜荞茎秆木质素合成关键酶基因的表达分析. 中国农业科学, 2015, 48(9): 1864-1872. HU D, LIU X B, WANG C, YANG H, LI H X, RUAN R W, YUAN X H, YI Z L. Expression analysis of key enzyme genes in lignin synthesis of culm among different lodging resistances of common buckwheat (Fagopyrum esculentum Moench). Scientia Agricultura Sinica, 2015, 48(9): 1864-1872. (in Chinese) 
[8] 李雪. 荞麦属植物落粒性相关基因研究[D]. 贵阳: 贵州师范大学, 2015. LI X. A study of genes related to seed shattering in buckwheat [D]. Guiyang: Guizhou Normal University, 2015. (in Chinese) 
[9] 汪灿, 阮仁武, 袁晓辉, 胡丹, 杨浩, 林婷婷, 何沛龙, 李燕, 易泽林. 荞麦茎秆解剖结构和木质素代谢及其与抗倒性的关系. 作物学报, 2014, 40(10): 1846-1856. WANG C, RUAN R W, YUAN X H, HU D, YANG H, LIN T T, HE P L, LI Y, YI Z L. Relationship of anatomical structure and lignin metabolism with lodging resistance of culm in buckwheat. Acta Agronomica Sinica, 2014, 40(10): 1846-1856. (in Chinese) 
[10] 赵涛, 高小丽, 高扬, 张东旗, 高金锋, 王鹏科, 杨璞. 轮作及连作条件下荞麦功能叶片衰老特性的比较. 西北农业学报, 2015, 24(11): 87-94. 
ZHAO T, GAO X L, GAO Y, ZHANG D Q, GAO J F, WANG P K, YANG P. The characteristic function of buckwheat leaf senescence under the conditions of rotation and continuous cropping. Acta Agriculturae Boreali-occidentalis Sinica, 2015, 24(11): 87-94. (in Chinese) 
[11] 高扬, 高小丽, 张东旗, 赵涛, 高金锋, 杨璞, 冯佰利. 连作对荞麦产量、土壤养分及酶活性的影响. 土壤, 2014, 46(6): 1091-1096. 
GAO Y, GAO X L, ZHANG D Q, ZHAO T, GAO J F, YANG P, FENG B L. Continuous cropping of buckwheat production, the influence of soil nutrient and enzyme activity. Soils, 2014, 46(6): 1091-1096. (in Chinese) 
[12] FAO (Food and Agriculture Organization of the United Nations ).http://www.fao.org/statistics/zh, 2014. 
FAO (Food and Agriculture Organization of the United Nations ).http://www.fao.org/statistics/zh, 2014. (in Chinese) 
[13] 邹俊林, 刘卫国, 袁晋, 蒋涛, 叶素琴, 邓榆川, 杨晨雨, 罗玲, 杨文钰. 套作大豆苗期茎秆木质素合成与抗倒性的关系. 作物学报, 2015, 41(7): 1098-1104. 
ZOU J L, LIU W G, YUAN J, JIANG T, YE S Q, DENG Y C, YANG C Y, LUO L, YANG W Y. Relationship between lignin synthesis and lodging resistance at seedlings stage in soybean intercropping system. Acta Agronomica Sinica, 2015, 41(7): 1098-1104. (in Chinese) 
[14] 高珍妮, 郭丽琢, 李丽, 郭芳, 牛俊义. 氮肥对胡麻茎秆木质素合成酶活性及其抗倒性的影响. 中国油料作物学报, 2014, 36(5): 610-615. 
GAO Z N, GUO L Z, LI L, GUO F, NIU J Y. Effects of nitrogen on oilseed flax stem lignin and relative enzyme and lodging resistance. Chinese Journal of Oil Crop Sciences, 2014, 36(5): 610-615. (in Chinese)
[15] 黄杰恒, 李威, 曲存民, 刘列钊, 徐新福, 王瑞, 李加纳. 甘蓝型油菜不同抗倒性材料中木质素代谢途径关键基因表达特点. 作物学报, 2013, 39(8): 1339-1344. 
HUANG J H, LI W, QU C M, LIU L Z, XU X F, WANG R, LI J N. Expression characteristics of key genes in lignin pathway among different lodging resistance lines of Brassica napus L.. Acta Agronomica Sinica, 2013, 39(8): 1339-1344. 
[16] 杨世民, 谢力, 郑顺林, 李静, 袁继超. 氮肥水平和栽插密度对杂交稻茎秆理化特性与抗倒伏性的影响. 作物学报, 2009, 35(1): 93-103. 
YANG S M, XIE L, ZHENG S L, LI J, YUAN J C. Effects of nitrogen rate and transplanting density on physical and chemical characteristics and lodging resistance of culms in hybrid rice. Acta Agronomica Sinica, 2009, 35(1): 93-103. (in Chinese) 
[17] BAUCHER M, MONTIES B, MONTAGU M V, BOERJAN W. Biosynthesis and genetic engineering of lignin. Critical Reviews in Plant Sciences, 2010, 17(2): 125-197. 
[18] BOERJAN W, RALPH J, BAUCHER M. Lignin biosynthesis. Annual Review of Plant Biology, 2003, 54(1): 519-546. 
[19] WHETTEN R, SEDEROFF R. Lignin biosynthesis. The Plant Cell, 1995, 7(7): 1001-1013. 
[20] 卢昆丽, 尹燕枰, 王振林, 李勇, 彭佃亮, 杨卫兵, 崔正勇, 杨东 清, 江文文. 施氮期对小麦茎秆木质素合成的影响及其抗倒伏生理机制. 作物学报, 2014, 40(9): 1686-1694. 
LU K L, YIN Y P, WANG Z P, Li Y, PENG D L, YANG W B, CUI Z Y, YANG D Q, JIANG W W. Effect of nitrogen fertilization timing on lignin synthesis of stem and physiological mechanism of lodging resistance in wheat. Acta Agronomica Sinica, 2014, 40(9): 1686-1694. (in Chinese) 
[21] 陈晓光, 石玉华, 王成雨, 尹燕枰, 宁堂原, 史春余, 李勇, 王振 林. 氮肥和多效唑对小麦茎秆木质素合成的影响及其与抗倒伏性的关系. 中国农业科学, 2011, 44(17): 3529-3536. 
CHEN X G, SHI Y H, WANG C Y, YIN Y P, NING T Y, SHI C Y, LI Y, WANG Z L. Effects of nitrogen and pp333 application on the lignin synthesis of stem in relation to lodging resistance of wheat. Scientia Agricultura Sinica, 2011, 44(17): 3529-3536. (in Chinese) 
[22] 李扬. 甘蓝型油菜倒伏相关性状QTL定位和木质素合成关键基因表达研究[D]. 南京: 南京农业大学, 2013. 
LI Y. QTL mapping of traits related to lodging resistance and expression of key gene in lignin synthesis in Brassica napus L. [D]. Nanjing: Nanjing Agricultural University, 2013. (in Chinese) 
[23] 殷静. 大豆茎倒伏突变体基因定位、转录组测序及2个木质素合成基因的克隆研究[D]. 南京: 南京农业大学, 2013. YIN J. Gene mapping, transcriptome sequencing and cloning of two lignin biosynthetic genes of a stem lodging mutant in soybean [D]. Nanjing: Nanjing Agricultural University, 2013. (in Chinese) 
[24] 陈晓光. 小麦茎秆木质素代谢及其与抗倒性的关系[D]. 泰安: 山东农业大学, 2011. 
CHEN X G. Relationship between lignin metabolism and lodging resistance in wheat [D]. Taian: Shandong Agricultural University, 2011. (in Chinese) 
[25] 杨理慧. 小麦木质素单体合成关键酶基因的克隆与生物信息学分析[D]. 郑州: 郑州大学, 2013. 
YANG L H. Cloning and bioinformatics analysis of the key enzyme genes related to lignin monomer synthesize in wheat [D]. Zhengzhou: Zhengzhou University, 2013. (in Chinese) 
[26] 师竹娟. 甘蓝型油菜木质素单体合成基因COMT和F5H的克隆及表达调控[D]. 北京: 中国农业科学院, 2006. 
SHI Z J. Monolignol biosynthesis gene COMT and F5H cloning and expression regulation in Brassica napus L.[D]. Beijing: Chinese Academy of Agricultural Sciences, 2006. (in Chinese) 
[27] 林葵, 黄祥辉, 王隆华, 李人圭, 颜季琼. 甜菜子叶不定芽分化过程中PAL活性和木质素含量变化研究. 华东师范大学学报(自然科学版), 1996(2): 92-97. 
LIN K, HUANG X H, WANG L H, LI R G, YAN J Q. The change of PAL activity and lignin content during adventitious buds formation of cotyledon in Cucumis melon L.. Journal of East China Normal University(Natural Science), 1996(2): 92-97. (in Chinese) 
[28] ROLANDO C, MONTIES B, LAPIERRE C. Thioacidolysis, Methods in Lignin Chemistry. Berlin Heidelberg: Springer Berlin Heidelberg, 1992: 334-349. 
[29] 彭佃亮. 小麦倒伏与茎杆木质素代谢的关系及其对籽粒产量和淀粉特征的影响[D]. 泰安: 山东农业大学, 2014. 
PENG D L. Effects of lodging on grain yield and starch characteristics and its relationship to stem lignin metabolism in wheat [D]. Taian: Shandong Agricultural University, 2014. (in Chinese) 
[30] 黄杰恒. 干旱胁迫下油菜抗倒伏相关性状动态变化及木质素关键基因表达特性分析[D]. 北碚: 西南大学, 2013. 
HUANG J H. Lodging resistant traits and lignin related gene analysis in B. napus under drought stress [D]. Beibei: Southwest University, 2013. (in Chinese) 
[31] JORGE R, ANA G, LIDIA N. Lignin composition and structure in young versus adult Eucalyptus globulus plants. Plant Physiology, 2011, 155(2): 667-682. 
[32] MAZUMDER B B, NAKGAWA A, KURODA K I. Evaluation of harvesting time effects on kenaf bast lignin by pyrolysis-gas chromatography. Industrial Crops & Products, 2005, 21(1): 17-24. 
[33] ROBINSON A R, MANSFIELD S D. Rapid analysis of poplar lignin monomer composition by a streamlined thioacidolysis procedure and near-infrared reflectance-based prediction modeling. The Plant Journal, 2009, 58(58): 706-714.
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