碱性双氧水预处理对苹果渣化学组分和酶解得率的影响

doi:10.3864/j.issn.0578-1752.2016.08.013

Abstract

Abstract: 【Objective】 The aim of this study was to research the impact of a 4.5% alkaline hydrogen peroxide (AHP) pretreatment at pH 11.5 on the chemical composition of apple pomace, the lignin removal rate, and the enzymatic hydrolyzability of cellulase. 【Method】 The temperature and time of pretreatment were optimized in accordance with the index of the lignin removal rate in this study. After filtering to collect solid components and drying, the AHP pretreated apple pomace was obtained. According to the content of cellulose and hemicellulose, the content of lignin, and total sugar content of enzymatic hydrolysis of apple pomace before AHP, the effects of different pretreated time on the content and recovery of cellulose and hemicellulose, the content and removal rate of lignin, and the enzymatic hydrolyzability were analyzed under the optimal pretreatment temperature. Utilizing a scanning electron microscope (SEM), thermogravimetry/differential thermogravimetry (TG/DTG), and fourier transform infrared (FTIR) characterized the physical and chemical composition change of apple pomace before and after AHP. 【Result】 The temperature and time of pretreatment had significant effects on the lignin removal rate. Considering various factors, especially the economic benefits, when the time was 2 hours and the temperature was 50℃, the lignin removal rate was optimal with 56.68%. The composition variation and enzymatic hydrolyzability of apple pomace after different pretreated times was analyzed under optimal pretreated temperature. When pretreated time was 2 hours, the recovery of cellulose and hemicellulose was 99.86%, which was very close to the content of untreated apple pomace; the enzymatic hydrolyzability was 0.54 g·g^-1, which was 200% of untreated apple pomace. The SEM images showed that the physical structure was multi-hole and loose, the fiber bundle became loose and rough, and the internal surface area was indefinitely increased after apple pomace treated with AHP. TG/DTG demonstrated that AHP could apparently increase the purity of cellulose monomer and reduce the content of lignin and residue. The FTIR study revealed that AHP could substantially disrupt the chemical structure of lignin, and the structure of guaiacyl, syringyl, and p-hydroxylphengl of lignin was destroyed. Thus, the lignin content of apple pomace was reduced after AHP. 【Conclusion】 This paper illustrated that AHP was an efficient pretreatment agent of apple pomace, and that the effect of pretreatment was closely associated with temperature and time.

Key words: apple pomace, alkaline hydrogen peroxide, enzymatic hydrolyzability, lignin, characteristic structure analysis

YOU Yi-na, DENG Hong, MENG Yong-hong, FANG Jie, GUO Yu-rong, ZHANG Ying . The Impact of Alkaline Hydrogen Peroxide Pretreatment on the Chemical Composition and Enzymatic Hydrolyzability of Apple Pomace[J].Scientia Agricultura Sinica, 2016, 49(8): 1559-1566.

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References

[1] 于滨, 吴茂玉, 朱凤涛, 和法涛. 苹果渣综合利用研究进展. 中国果蔬, 2012(12): 31-34.

Yu B,Wu M Y, Zhu F T, He F T. Comprehensive utilization of apple pomace: A review. China Fruit and Vegetable, 2012(12): 31-34. (in Chinese)

[2] Pauly M, Keegstra K. Plant cell wall polymers as precursors for biofuels. Current Opinion in Plant Biology, 2010, 13(3): 304-311.

[3] Da Costa Correia J A, Júnior J E M, Gonçalves L R B, Rocha M V P. Alkaline hydrogen peroxide pretreatment of cashew apple bagasse for ethanol production: study of parameters. Bioresource Technology, 2013, 139: 249-256.

[4] Lynd L R, Laser M S, Bransby D, Dale B E, Davison B, Hamilton R, Himmel M, Keller M, McMillan J D, Sheehan J, Wyman C E. How biotech can transform biofuels. Nature Biotechnology, 2008, 26(2): 169-172.

[5] Chundawat S P S, Donohoe B S, da Costa Sousa L, Elder T, Agarwal U P, Lu F, Ralph J, Himmel M E, Balan V, Dale B E. Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment. Energy & Environmental Science, 2011, 4(3): 973-984.

[6] Gould J M. Studies on the mechanism of alkaline peroxide delignification of agricultural residues. Biotechnology and Bioengineering, 1985, 27(3): 225-231.

[7] Rabelo S C, Maciel Filho R, Costa A C. A comparison between lime and alkaline hydrogen peroxide pretreatments of sugarcane bagasse for ethanol production. Applied Biochemistry and Biotechnology, 2008, 148: 45-58.

[8] Rabelo S C, Maciel Filho R, Costa A C. Lime pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse. Applied Biochemistry and Biotechnology, 2013, 169(5): 1696-1712.

[9] Patel M M, Bhatt R M. Optimisation of the alkaline peroxide pretreatment for the delignification of rice straw and its applications. Journal of Chemical Technology and Biotechnology, 1992, 53(3): 253-263.

[10] Banerjee G, Car S, Scott-Craig J S, Hodge D B, Walton J D. Alkaline peroxide pretreatment of corn stover: effects of biomass, peroxide, and enzyme loading and composition on yields of glucose and xylose. Biotechnol Biofuels, 2011, 4(1): 1-16.

[11] Balat M. Production of bioethanol from lignocellulosic materials via the biochemical pathway: A review. Energy Conversion and Management, 2011, 52(2): 858-875.

[12] Cao W, Sun C, Liu R, Yin R, Wu X. Comparison of the effects of five pretreatment methods on enhancing the enzymatic digestibility and ethanol production from sweet sorghum bagasse. Bioresource Technology, 2012, 111: 215-221.

[13] Alvarez-Vasco C, Zhang X. Alkaline hydrogen peroxide pretreatment of softwood: Hemicellulose degradation pathways. Bioresource Technology, 2013, 150: 321-327.

[14] Da Costa J A, Marques J J E, Gonçalves L R B, Rocha M V P. Enhanced enzymatic hydrolysis and ethanol production from cashew apple bagasse pretreated with alkaline hydrogen peroxide. Bioresource Technology, 2015, 179: 249-259.

[15] Shao Q, Cheng C, Ong R G, Zhu L, Zhao C. Hydrogen peroxide presoaking of bamboo prior to AFEX pretreatment and impact on enzymatic conversion to fermentable sugars. Bioresource Technology, 2013, 142: 26-31.

[16] Diaz A, Le Toullec J, Blandino A, De Ory I, Caro I. Pretreatment of rice hulls with alkaline peroxide to enhance enzyme hydrolysis for ethanol production. Chemical Engineering Transactions, 2013, 32: 949-954.

[17] Michalska K, Ledakowicz S. Alkali pre-treatment of Sorghum Moench for biogas production. Chemical Papers, 2013, 67(9): 1130-1137.

[18] Ayeni A O, Hymore F K, Mudliar S N, Deshmukh S C, Satpute D B, Omoleye J A, Pandey R A. Hydrogen peroxide and lime based oxidative pretreatment of wood waste to enhance enzymatic hydrolysis for a biorefinery: Process parameters optimization using response surface methodology. Fuel, 2013, 106: 187-194.

[19] Miller G L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 1959, 31(3): 426-428.

[20] Harrison M D, Zhang Z, Shand K, O’Hara I M, Doherty W O, Dale J L. Effect of pretreatment on saccharification of sugarcane bagasse by complex and simple enzyme mixtures. Bioresource Technology, 2013, 148: 105-113.

[21] Zhang Z, O’Hara I M, Doherty W O S. Pretreatment of sugarcane bagasse by acidified aqueous polyol solutions. Cellulose, 2013, 20(6): 3179-3190.

[22] Zhang J, Feng L, Wang D, Zhang R, Liu G, Cheng G. Thermogravimetric analysis of lignocellulosic biomass with ionic liquid pretreatment. Bioresource Technology, 2014, 153: 379-382.

[23] Singh S, Varanasi P, Singh P, Adams P D, Auer M, Simmons B A. Understanding the impact of ionic liquid pretreatment on cellulose and lignin via thermochemical analysis. Biomass and Bioenergy, 2013, 54: 276-283.

[24] Gao A H, Bule M V, Laskar D D, Chen S. Structural and thermal characterization of wheat straw pretreated with aqueous ammonia soaking. Journal of Agricultural and Food Chemistry, 2012, 60(35): 8632-8639.

[25] Pandey K K, Pitman A J. FTIR studies of the changes in wood chemistry following decay by brown-rot and white-rot fungi. International Biodeterioration & Biodegradation, 2003, 52(3): 151-160.

[26] Yu G, Afzal W, Yang F, Padmanabhan S, Liu Z, Xie H, Shafy M A, Bell A T, Prausnitz J M. Pretreatment of Miscanthus×giganteus using aqueous ammonia with hydrogen peroxide to increase enzymatic hydrolysis to sugars. Journal of Chemical Technology and Biotechnology, 2014, 89(5): 698-706.

[27] Singh D, Zeng J, Laskar D D, Deobald L, Hiscox W C, Chen S. Investigation of wheat straw biodegradation by phanerochaete chrysosporium. Biomass and Bioenergy, 2011, 35(3): 1030-1040.

[28] Lawther J M, Sun R, Banks W B. Fractional characterization of wheat straw lignin components by alkaline nitrobenzene oxidation and FT-IR spectroscopy. Journal of Agricultural and Food Chemistry, 1996, 44(5): 1241-1247.

[29] Singh J, Suhag M, Dhaka A. Augmented digestion of lignocellulose by steam explosion, acid and alkaline pretreatment methods: A review. Carbohydrate Polymers, 2015, 117: 624-631.

[30] Prasad S, Singh A, Jain N, Joshi H C. Ethanol production from sweet sorghum syrup for utilization as automotive fuel in India. Energy & Fuels, 2007, 21(4): 2415-2420.

[31] Kumar A, Singh L K, Ghosh S. Bioconversion of lignocellulosic fraction of water-hyacinth (Eichhornia crassipes) hemicellulose acid hydrolysate to ethanol by Pichia stipitis. Bioresource Technology, 2009, 100(13): 3293-3297.

[32] Rabelo S C, Filho R M, Costa A C. A comparison between lime and alkaline hydrogen peroxide pretreatments of sugarcane bagasse for ethanol production. Applied Biochemistry and Biotechnology, 2008, 148: 563-576.

[33] 耿乙文, 哈益明, 靳婧, 李庆鹏. 过氧化氢改性苹果渣膳食纤维的研究. 中国农业科学, 2015, 48(19): 3979-3988.

Geng Y W, Ha Y M, Jin J, Li Q P. Apple pomace dietary fibre modification by hydrogen peroxide. Scientia Agricultura Sinica, 2015, 48(19): 3979-3988. (in Chinese)

[34] Sun C, Liu R, Cao W, Yin R, Mei Y, Zhang L. Impacts of alkaline hydrogen peroxide pretreatment on chemical composition and biochemical methane potential of agricultural crop stalks. Energy & Fuels, 2015, 29(8): 4966-4975.

[35] Silverstein R A, Chen Y, Sharma-Shivappa R R, Boyette M D, Osborne J. A comparison of chemical pretreatment methods for improving saccharification of cotton stalks. Bioresource Technology, 2007, 98(16): 3000-3011.

[36] Agbor V B, Cicek N, Sparling R, Berlin A, Levin D B. Biomass pretreatment: fundamentals toward application. Biotechnology Advances, 2011, 29(6): 675-685.

[37] Pan X, Xie D, Yu R W, Lam D, Saddler J N. Pretreatment of lodgepole pine killed by mountain pine beetle using the ethanol organosolv process: Fractionation and process optimization. Industrial & Engineering Chemistry Research, 2007, 46(8): 2609-2617.

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