24-表油菜素内酯对葡萄叶片抵御霜霉菌侵染的影响

doi:10.3864/j.issn.0578-1752.2016.15.015

Abstract

Abstract: 【Objective】 The study researched the effect of24-epibrassinolide (EBR) treatment on the resistance in grapevine leaf against Plasmopara viticolaand the mechanism of the induced resistance. 【Method】 Cabernet Sauvignon (Vitis vinifera L.) grapevine leaves were used for experimental materials. At the early development of P. viticola invading grapevine leaf, the effect of different concentration of exogenous EBR (0.1, 0.5 and 1.0 mg·L^-1 EBR) on the disease incidence and severity of downy mildew of grapevine leaves, linear length of colonies per infection, the development of P. viticola sporangiophores while this pathogen invading the grapevine leaf, the number of spores per stoma, the stomatal aperture and the content of endogenous hormone and the relation between hormone content and stomatal aperture were investigated. 【Result】 EBR-treated leaves had a significantly lower stomatal aperture at 0.5 h after inoculation. Both on 1 d and 2 d, a significantly lower linear length of P. viticola colonies per infection was observed in all EBR treatments. The spread of mycelium almost covered most of the infection area in control while 0.5 and 1.0 mg·L^-1 EBR treated leaf resulted in a markedly restricted number of spores per stoma and growth of P. viticola after 3 days of inoculation. After 4 days of inoculation, EBR treatment significantly controlled the development ofP. viticola sporangiophores, disease incidence and disease severity, and 0.5 mg·L^-1 and 1.0 mg·L^-1 EBR treatment resulted in higher resistance. The leaves treated with 0.5 mg·L^-1 and 1.0 mg·L^-1 had a lower disease incidence and severity of downy mildew, while disease incidence was decreased by 51.4% and 45.0%，and disease severity drop by 71.2% and 62.9%, and there is no significance between the two treatment. There was a significant difference in ABA, JA and SA contents in grape leaves between CK and 0.5 mg·L^-1 EBR treatment. Stomatal aperture has significantly positive correlation with SA content while ABA content does with JA content. 【Conclusion】 The increased resistance against P. viticola invasion was possibly related with the suppression of pathogen development and the stomata closure which plant hormone crosstalk involved in.

Key words: grapevine, Plasmopara viticola, 24-epibrassinolide, induced resistance, stomata aperture

LIU Qing, LUAN Xue-tao, XU Shi-yan,MENG Ying, GAO Jiang-man, XI Zhu-mei. Effect of 24-epibrassinolide Treatment on Grapevine Leaf Against Plasmopara viticola[J].Scientia Agricultura Sinica, 2016, 49(15): 3010-3018.

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