寡糖·链蛋白对小麦抗黄花叶病毒的免疫诱抗作用

doi:10.3864/j.issn.0578-1752.2016.18.010

Abstract

Abstract: 【Objective】Plant activator could trigger effective, persistent and broad-spectrum resistance in plants. The objective of this experiment is to study the resistance induced by oligosaccharins·plant activator protein against wheat yellow mosaic virus (WYMV), thus providing a technical basis for the research and promotion of the use of activator proteins on a large scale.【Method】In laboratory, WYMV susceptible wheat cultivar ‘Aikang 58’ was sowed in the soil that was collected from the WYMV infested field and grown in the incubator under (27±2)℃. When the wheat grown up to five-leaf stage, the 6% oligosaccharins·plant activator protein that was diluted 1 000 times was sprayed. After 7 days, the wheat was put in incubator for 30 days under (12±1)℃, so as to get WYMV inoculated plants. Then the height of wheat plant, chlorophyll content, disease index, and control efficiency were measured or calculated. In filed, wheat was sowed in the plot that infested with WYMV for many years. The oligosaccharins·plant activator protein was sprayed once a week for successive three times of spray when wheat plant was at returning green stage. At the same time, the height of wheat plant, chlorophyll content, disease index, and control efficiency were measured or calculated. Moreover, 20 upper first fully expanded leaves in each plot were taken every week to detect the copy number of WYMV-CP by qPCR during the field investigation. The thousand grain weight, the seeds per ear and the yields were measured and calculated at harvest.【Result】The laboratory experiment showed that after 30 days of the activator spray, the chlorophyll content increased dramatically (P＜0.05), the disease index decreased as the result of the control efficiency reached up to 63.32%, but no difference was found in plant height compared to the control. The field experiment showed that no significant control efficiency after one time spray, but the control efficiency reached up to 46.67% after two times of spray. The suppression rate of the copy number of WYMV-CP reached up to 69.30% in the first week, 85.50% in the second week, and the highest suppression reached up to 99.20% in the third week. However, the chlorophyll content and the height of wheat plants showed no significant differences. Although the seed numbers per ear significantly increased, but no difference was observed in thousand grain weight. As a result, the yields were significantly increased.【Conclusion】Oligosaccharins·plant activator protein could induce wheat plant resistance to WYMV. The copy number of WYMV-CP dramatically decreased in activator protein treated plants. It was showed that the activator could relieve WYMV damage and reduce yield loses in wheat fields.

Key words: Wheat yellow mosaic virus (WYMV), oligosaccharins·plant activator protein, induced resistance, activator, WYMV-CP

XU Run-dong, SHENG Shi-ying, YANG Xiu-fen, LIU Yong. Effect of Induced Resistance of Oligosaccharins·Plant Activator Protein on Wheat to WYMV[J].Scientia Agricultura Sinica, 2016, 49(18): 3561-3568.

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